Miyakogusa Predicted Gene

Lj0g3v0129059.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0129059.1 tr|Q40360|Q40360_MEDSA NADH-dependent glutamate
synthase OS=Medicago sativa PE=4 SV=1,91.8,0,FMN-linked
oxidoreductases,NULL; N-terminal nucleophile aminohydrolases (Ntn
hydrolases),NULL; Alpha,CUFF.7971.1
         (1514 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr1g027020.1 | NADH glutamate synthase | HC | chr1:8906141-89...  2742   0.0  
Medtr7g089970.1 | ferredoxin-dependent glutamate synthase | HC |...  1075   0.0  

>Medtr1g027020.1 | NADH glutamate synthase | HC | chr1:8906141-8918572
            | 20130731
          Length = 2194

 Score = 2742 bits (7108), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 1329/1514 (87%), Positives = 1383/1514 (91%), Gaps = 2/1514 (0%)

Query: 1    MFFLPKSDNRRKESKNIFRKVAESMGLSILGWRSVRTDNTGLGKSALQTEPVIEQVFLTA 60
            MFFLPKS +RRKESKNIF KVAES+G  +LGWRSV TDNTGLGKSA  TEPVIEQVFLT 
Sbjct: 174  MFFLPKSGSRRKESKNIFTKVAESLGHKVLGWRSVPTDNTGLGKSAQLTEPVIEQVFLTP 233

Query: 61   SGKSKVDLERQMYILRKLSMAAITSALNLQNDGIADFYICSLSSRTVVYKGQLTPAQLRE 120
            S  SKVDLE+QMYILRKLSM +ITSALNLQ+DGI DFYICSLSSRTV+YKGQLTPAQL E
Sbjct: 234  SSDSKVDLEKQMYILRKLSMVSITSALNLQSDGITDFYICSLSSRTVIYKGQLTPAQLGE 293

Query: 121  YYYADLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMKAREG 180
            YYYADLGNERFTSYMALIHSRFSTNTFPSWDRAQP RVLGHNGEINTLRGNVNW+KAREG
Sbjct: 294  YYYADLGNERFTSYMALIHSRFSTNTFPSWDRAQPFRVLGHNGEINTLRGNVNWIKAREG 353

Query: 181  LLKCKELGLSENELKKLLPIVDANSSDSGAFDGVLEFLVHSGKSLPEAVMMMIPEAWQKD 240
            LLKCKELGLSEN+LKK LPIVDANSSDSG FDGVLEFL+HSGKSLPEAVMMMIPEAWQ D
Sbjct: 354  LLKCKELGLSENDLKKFLPIVDANSSDSGCFDGVLEFLLHSGKSLPEAVMMMIPEAWQND 413

Query: 241  KNMDPQRKAFYEYYSALMEPWDGPALISFTDGHYLGATLDRNGLRPGRFYVTHSGRVIMA 300
            KNMDPQRKAFYEYYSALMEPWDGPALISFTDGHYLGATLDRNGLRPGRFYVTHSGRVIMA
Sbjct: 414  KNMDPQRKAFYEYYSALMEPWDGPALISFTDGHYLGATLDRNGLRPGRFYVTHSGRVIMA 473

Query: 301  SEVGVVDIPPEDVRRKGRLNPGMMLLVDFEKHIVVNDDALKEQYSLERPYGDWLKKQKID 360
            SEVGVVDIPPEDV RKGRLNPGMMLLVDFEK IVVNDDALKEQYSL RPYGDWL+KQKI+
Sbjct: 474  SEVGVVDIPPEDVCRKGRLNPGMMLLVDFEKQIVVNDDALKEQYSLARPYGDWLEKQKIE 533

Query: 361  LKDIVDSVHESERVPPTITGVAPLSCDDVDMENMGIHGLLAPLKVFGYTVESLEMLLLPM 420
            LKDIVDSVHES+ VPPTI+GV PLS DDVDMENMGI GLLAPLK FGY+VESLE+LLLPM
Sbjct: 534  LKDIVDSVHESDIVPPTISGVPPLSNDDVDMENMGIQGLLAPLKAFGYSVESLEILLLPM 593

Query: 421  AKDGVEALGSMGNDTPLAVMSNREKLSFEYFKQMFAQVTNPPIDPIREKIVTSMECMVGP 480
            AKDGVEALGSMGNDTPLAVMSNREKL+FEYFKQMFAQVTNPPIDPIREKIVTSM CMVGP
Sbjct: 594  AKDGVEALGSMGNDTPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMRCMVGP 653

Query: 481  EGDLTETTEEQCHRLSLKGPLLSTEQMEAIKKMDYRGWRSKVIDITYSKERGKRGLEEAL 540
            EGDLTETTEEQCHRLSLKGPLLST++MEAIKKM+YRGWRSKVIDITYSKERGK+GLEEAL
Sbjct: 654  EGDLTETTEEQCHRLSLKGPLLSTKEMEAIKKMNYRGWRSKVIDITYSKERGKKGLEEAL 713

Query: 541  DRICAEAHGAIKEGYTTLVLSDRAFSRKRXXXXXXXXXXXXHQHLVKTLERTRVALMIES 600
            DRIC EAH AI EGYTTLVLSDRAFS+KR            HQHLVKTLERTRVALM+ES
Sbjct: 714  DRICTEAHNAISEGYTTLVLSDRAFSKKRVAVSSLLAVGAVHQHLVKTLERTRVALMVES 773

Query: 601  AEPREVHHFCTLVGFGADAICPYLSIEAIWRLQVDGKIPPKANGVFHSKDELVKKYFKAS 660
            AEPREVHHFCTLVGFGADAICPYL+IEAIWRLQVDGKIPPKA+G F+SKDELVKKYFKAS
Sbjct: 774  AEPREVHHFCTLVGFGADAICPYLAIEAIWRLQVDGKIPPKASGDFNSKDELVKKYFKAS 833

Query: 661  HYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIDKCFAGTPSRVEGATFEMLSSDALQ 720
             YGMMKVLAKMGISTLASYKGAQIFEALGLSSEVI+KCFAGTPSRVEGATFEML+ DAL 
Sbjct: 834  TYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIEKCFAGTPSRVEGATFEMLAQDALD 893

Query: 721  LHELAFPSRTFSPGSAEAVALPNPGDYHWRKGGEVHLNDPLAIAKLQEAARTNSVDAYKQ 780
            LHELAFPSR FSPGSAEAVALPNPGDYHWRKGGEVHLNDPLAIAKLQEAARTNSV+AYKQ
Sbjct: 894  LHELAFPSRNFSPGSAEAVALPNPGDYHWRKGGEVHLNDPLAIAKLQEAARTNSVEAYKQ 953

Query: 781  YSKIIHELNKACNLRGLLKFKETSAKISIDEVEPASEIVKRFCTGAMSYGSISLEAHSTL 840
            YSKIIHELNKACNLRGLLKFK+ ++K+ I EVEPASEIVKRFCTGAMSYGSISLEAH+ L
Sbjct: 954  YSKIIHELNKACNLRGLLKFKDAASKVPISEVEPASEIVKRFCTGAMSYGSISLEAHTAL 1013

Query: 841  ALAMNKIGGKSNTGEGGEQPSRMEPLADGSRNPKRSAIKQVASGRFGVSSYYLTNADELQ 900
            A AMNKIGGKSNTGEGGEQPSRMEPLADGSRNPKRSAIKQVASGRFGVSSYYLTNADELQ
Sbjct: 1014 ATAMNKIGGKSNTGEGGEQPSRMEPLADGSRNPKRSAIKQVASGRFGVSSYYLTNADELQ 1073

Query: 901  IKMAQGAKPGEGGELPGHKVVGDIAVTRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKN 960
            IKMAQGAKPGEGGELPGHKV+GDIA+TRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKN
Sbjct: 1074 IKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKN 1133

Query: 961  ANPAARISVKLVSEAGVGIIASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGL 1020
            ANPAARISVKLVSEAGVG+IASGVVKGHA+HVLISGHDGGTGASRWTGIK+AGLPWELGL
Sbjct: 1134 ANPAARISVKLVSEAGVGVIASGVVKGHAEHVLISGHDGGTGASRWTGIKSAGLPWELGL 1193

Query: 1021 AETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAPLITLGCIMMRKCH 1080
            AETHQTLVANDLRGRT LQTDGQLKTGRDVAIAALLGAEE+GFSTAPLITLGCIMMRKCH
Sbjct: 1194 AETHQTLVANDLRGRTTLQTDGQLKTGRDVAIAALLGAEEYGFSTAPLITLGCIMMRKCH 1253

Query: 1081 KNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMAQLGFRTVNEMVGHSDMLE 1140
            KNTCPVGIATQDPVLREKFAGEPEHVINF FMVAEEMREIM+QLGFRTVNEMVG SDMLE
Sbjct: 1254 KNTCPVGIATQDPVLREKFAGEPEHVINFLFMVAEEMREIMSQLGFRTVNEMVGRSDMLE 1313

Query: 1141 VDKEVVKSNEKLENIDLSLLLRPAAELRPEAAQYCVQKQDHSLDMALDNQLISLSSAALE 1200
            VDKEVVK N KLENIDLSLLLRPAAELRPEAAQYCVQKQDH LDMALDN+LISLS+AALE
Sbjct: 1314 VDKEVVKGNAKLENIDLSLLLRPAAELRPEAAQYCVQKQDHGLDMALDNKLISLSNAALE 1373

Query: 1201 KGLPVYIETPIYNVNRAVGTMLSHEVTKRYHLAGLPTDTIHIKFNGSAGQSFGAFLCPGI 1260
            KGLPVYIETPI N NRAVGTMLSHEVTKRY+LAGLP DTIHI+F GSAGQSFGAFLCPGI
Sbjct: 1374 KGLPVYIETPICNTNRAVGTMLSHEVTKRYNLAGLPADTIHIQFTGSAGQSFGAFLCPGI 1433

Query: 1261 TLELEGDSNDYXXXXXXXXXXXXYPPKGSNFDPKENIVIGNVALYGATRGEAYFNGMAAE 1320
            TLELEGDSNDY            YPPKGSNFDPK+NI+IGNVALYGATRGEAYFNGMAAE
Sbjct: 1434 TLELEGDSNDYIGKGLSGGKVVVYPPKGSNFDPKDNILIGNVALYGATRGEAYFNGMAAE 1493

Query: 1321 RFCVRNSGAKAVVEGVGDHGCEYMXXXXXXXXXXXXRNFAAGMSGGIAYVLNADGKFQSR 1380
            RFCVRNSGA+AVVEGVGDHGCEYM            RNFAAGMSGGIAYVL+ DG FQSR
Sbjct: 1494 RFCVRNSGAQAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGTFQSR 1553

Query: 1381 CNXXXXXXXXXXXXXXXXTLKMLIQQHQRHTNSLLAKEVLDNFGNLLPKFVKVIPREYKR 1440
            CN                TL+MLIQQHQRHTNSLLAKEVL +F NLLPKFVKV PREYKR
Sbjct: 1554 CNLELVDLDKVEEEEDIITLRMLIQQHQRHTNSLLAKEVLVDFDNLLPKFVKVFPREYKR 1613

Query: 1441 VLASMKSEEASKDAVERTXXXXXXXXXXXXXXXXXXFEELKKLATASLKEKQSEAESPKR 1500
            VLASMKS+ ASKDAVE                    FEELKKLATASL EK SEA  PKR
Sbjct: 1614 VLASMKSDAASKDAVESAAEDVDEQDDEAQAVEKDAFEELKKLATASLNEKPSEA--PKR 1671

Query: 1501 PSQVTEAIKHRGFV 1514
            PSQVT+A+KHRGFV
Sbjct: 1672 PSQVTDAVKHRGFV 1685


>Medtr7g089970.1 | ferredoxin-dependent glutamate synthase | HC |
            chr7:35266109-35288293 | 20130731
          Length = 1612

 Score = 1075 bits (2779), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 610/1474 (41%), Positives = 864/1474 (58%), Gaps = 97/1474 (6%)

Query: 1    MFFLPKSDNRRKESKNIFRKVAESMGLSILGWRSVRTDNTGLGKSALQTEPVIEQVFLTA 60
            M FLPK      ++K +     +  GL +LGWR V  + + +G  A +T P I+QVF+  
Sbjct: 171  MVFLPKDVELMNKAKKVIVNTFQQEGLEVLGWRPVPVNTSVVGYYAKETMPNIQQVFVKI 230

Query: 61   SGKSKV-DLERQMYILRKLSMAAITSALNLQNDGIADFYICSLSSRTVVYKGQLTPAQLR 119
              +    D+ER++YI RKL    ++S    ++ G  + Y CSLS+RT+VYKG L  +++ 
Sbjct: 231  GKEENTEDIERELYICRKLIEKEVSS----ESWG-NELYFCSLSNRTIVYKGMLR-SEVL 284

Query: 120  EYYYADLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMKARE 179
              +Y+DL N+ + S  A+ H R+STNT P W  AQPMR+LGHNGEINT++GN+NWM++RE
Sbjct: 285  GLFYSDLQNDLYKSSFAIYHRRYSTNTSPRWPLAQPMRLLGHNGEINTIQGNLNWMQSRE 344

Query: 180  GLLKCKELGLSENELKKLLPIVDANSSDSGAFDGVLEFLVHSGKSLPEAVMMMIPEAWQK 239
              LK       ENE++   P  +  +SDS   D   E L+ SG++  E++M+++PEA++ 
Sbjct: 345  PSLKSPVWRGRENEIR---PFGNPKASDSANLDSAAELLIRSGRTPEESMMILVPEAYKN 401

Query: 240  DKNMD---PQRKAFYEYYSALMEPWDGPALISFTDGHYLGATLDRNGLRPGRFYVTHSGR 296
               +    P+   FY+YY   ME WDGPAL+ F+DG  +GA LDRNGLRP R++ T    
Sbjct: 402  HPTLTIKYPEAIDFYDYYKGQMEAWDGPALLLFSDGKTVGACLDRNGLRPARYWRTSDNM 461

Query: 297  VIMASEVGVVDIPPEDVRRKGRLNPGMMLLVDFEKHIVVNDDALKEQYSLERPYGDWLKK 356
            V +ASEVGVV +    V  KGRL PGMM+ VD     V  +  +K++ +L  PYG+W+K+
Sbjct: 462  VYVASEVGVVPVDESKVISKGRLGPGMMITVDLLGGQVYENMEVKKRVALSNPYGNWIKE 521

Query: 357  QKIDLKD---IVDSVHESERVPPTITGVAPLSCDDVDMENMGIHGLLAPLKVFGYTVESL 413
                LK    +  SV E++ V                         L   + FGY+ E +
Sbjct: 522  NLRSLKSENFLSSSVMENDAV-------------------------LRHQQAFGYSSEDV 556

Query: 414  EMLLLPMAKDGVEALGSMGNDTPLAVMSNREKLSFEYFKQMFAQVTNPPIDPIREKIVTS 473
            +M++  MA  G E    MG+D PLA +S +  + F+YFKQ FAQVTNP IDP+RE +V S
Sbjct: 557  QMVIESMASQGKEPTFCMGDDIPLAALSQKPHMLFDYFKQRFAQVTNPAIDPLREGLVMS 616

Query: 474  MECMVGPEGDLTETTEEQCHRLSLKGPLLSTEQMEAIKKMDYRGWRSKVIDITYSKERGK 533
            +E  +G   ++ E   E   ++ L  P+L+  ++E++ K  +   + +V+   +   +G 
Sbjct: 617  LEVNIGKRRNILEIGPENASQVILSSPVLNEGELESLLKDSH--LKPQVLHTFFDITKGI 674

Query: 534  RG-LEEALDRICAEAHGAIKEGYTTLVLSDR--AFSRKRXXXXXXXXXXXXHQHLVKTLE 590
             G LE+AL+++C  A  A++ G   LVLSDR  A                 HQHL++   
Sbjct: 675  DGSLEKALNKLCDAADEAVRNGSQLLVLSDRSEALEPTHPAIPILLAVGTVHQHLIQNGL 734

Query: 591  RTRVALMIESAEPREVHHFCTLVGFGADAICPYLSIEAI--WRLQ-------VDGKIPPK 641
            R   +++ ++++    H F  L+G+GA A+CPYL++E    WRL         +GK+P  
Sbjct: 735  RMSASIVADTSQCFSTHQFACLIGYGASAVCPYLALETCRQWRLSNKTVNLMKNGKMPTV 794

Query: 642  ANGVFHSKDELVKKYFKASHYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIDKCFAG 701
                  S ++  K Y KA   G++K+L+KMGIS L+SY GAQIFE  GL  EV+D  F+G
Sbjct: 795  ------SIEQAQKNYCKAVKAGLLKILSKMGISLLSSYCGAQIFEIYGLGKEVVDLAFSG 848

Query: 702  TPSRVEGATFEMLSSDALQLHELAFPSRTFSPGSAEAVALPNPGDYHWRKGGEVHLNDPL 761
            + S++ G TF     D L    L+F  + FS  +A+   L N G   +R GGE H N+P 
Sbjct: 849  SVSKIGGLTF-----DELARETLSFWVKAFSEDTAKR--LENFGFIVFRPGGEYHANNPE 901

Query: 762  AIAKLQEAARTNSVDAYKQYSKIIHELNKACN-LRGLLKFKETSAKISIDEVEPASEIVK 820
                L +A R  S +A+  Y + +   N+  N +R LL+FK   A I + +VEPA  IVK
Sbjct: 902  MSKLLHKAVRQKSQNAFSVYQQYLA--NRPVNVIRDLLEFKSDRAPIPVGKVEPALSIVK 959

Query: 821  RFCTGAMSYGSISLEAHSTLALAMNKIGGKSNTGEGGEQPSRMEPLAD------------ 868
            RFCTG MS G+IS E H  +A+AMN+IGGKSN+GEGGE P R +PL D            
Sbjct: 960  RFCTGGMSLGAISRETHEAIAIAMNRIGGKSNSGEGGEDPIRWKPLTDVVDGYSPTLPHL 1019

Query: 869  -GSRN--PKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGDIA 925
             G +N     SAIKQVASGRFGV+  +L NAD+L+IK+AQGAKPGEGG+LPG KV   IA
Sbjct: 1020 KGLQNGDTATSAIKQVASGRFGVTPTFLANADQLEIKIAQGAKPGEGGQLPGKKVSMYIA 1079

Query: 926  VTRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPAARISVKLVSEAGVGIIASGVV 985
              RNS  GV LISPPPHHDIYSIEDLAQLI DL   NP A++SVKLV+EAG+G +ASGV 
Sbjct: 1080 RLRNSKPGVPLISPPPHHDIYSIEDLAQLIFDLHQVNPKAKVSVKLVAEAGIGTVASGVA 1139

Query: 986  KGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLK 1045
            KG+AD + ISGHDGGTGAS  + IK+AG PWELGL E+HQTLV N LR R +L+ DG  +
Sbjct: 1140 KGNADIIQISGHDGGTGASPISSIKHAGGPWELGLTESHQTLVENGLRERVILRVDGGFR 1199

Query: 1046 TGRDVAIAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEH 1105
            +G DV +AA++GA+E+GF +  +I  GC+M R CH N CPVG+A+Q   LR +F G P  
Sbjct: 1200 SGVDVMMAAIMGADEYGFGSVAMIATGCVMARICHTNNCPVGVASQREELRARFPGVPGD 1259

Query: 1106 VINFFFMVAEEMREIMAQLGFRTVNEMVGHSDMLEV-DKEVVKSNEKLENIDLSLLLRPA 1164
            ++N F  VAEE+R  +AQLG+  +++++G +++L   D  +VK+    +++DLS +L   
Sbjct: 1260 LVNLFLYVAEEVRGTLAQLGYEKLDDIIGRTELLRPRDVSLVKT----QHLDLSYILSNV 1315

Query: 1165 AELRPEAAQYCVQKQD-HSLDMALDNQLISLS--SAALEKGLPVYIETPIYNVNRAVGTM 1221
                P+ +   ++ Q+ H+    LD+ L++    + A+E    V     IYNV+R+    
Sbjct: 1316 G--LPKLSSTEIRNQEPHTNGPVLDDVLLADPKIADAIENEKAVSKTIKIYNVDRSACGR 1373

Query: 1222 LSHEVTKRYHLAGLPTDTIHIKFNGSAGQSFGAFLCPGITLELEGDSNDYXXXXXXXXXX 1281
            ++  + K+Y   G     ++I F GSAGQSFG FL PG+ + L G++NDY          
Sbjct: 1374 IAGVIAKKYGDTGF-AGQLNITFTGSAGQSFGCFLTPGMNIRLVGEANDYVGKGIAGGEL 1432

Query: 1282 XXYPPKGSNFDPKENIVIGNVALYGATRGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGC 1341
               P     F P++  ++GN  LYGAT G+ +  G A ERF VRNS A+AVVEG GDH C
Sbjct: 1433 VVTPVDKIGFQPEDAAIVGNTCLYGATGGQVFVRGKAGERFAVRNSLAEAVVEGAGDHCC 1492

Query: 1342 EYMXXXXXXXXXXXXRNFAAGMSGGIAYVLNADGKFQSRCNXXXXXXXXXXXXXXXXTLK 1401
            EYM            RN AAGM+GG+AY+L+ D     + N                 LK
Sbjct: 1493 EYMTGGCVVILGNVGRNVAAGMTGGLAYILDEDNTLIPKINREIVKIQRVTAPVGQIQLK 1552

Query: 1402 MLIQQHQRHTNSLLAKEVLDNFGNLLPKFVKVIP 1435
             LI+ H   T S   + +L ++   L  F +++P
Sbjct: 1553 KLIEAHVEKTGSNKGEAILKDWDKYLSLFWQLVP 1586