Miyakogusa Predicted Gene

Lj0g3v0072889.2
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0072889.2 Non Characterized Hit- tr|I1M329|I1M329_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.36912
PE,84.02,0,EF-hand, calcium binding motif,Calcium-binding EF-hand;
seg,NULL; EF-hand,NULL; EF_HAND_2,EF-HAND 2;,CUFF.3648.2
         (572 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr2g078240.1 | calcium-binding EF-hand protein | HC | chr2:32...   694   0.0  
Medtr2g078350.1 | calcium-binding EF-hand protein | HC | chr2:32...   182   6e-46
Medtr2g078250.1 | calcium ion-binding protein, putative | HC | c...   171   1e-42
Medtr2g078340.1 | calcium-binding EF-hand protein | HC | chr2:32...    70   5e-12

>Medtr2g078240.1 | calcium-binding EF-hand protein | HC |
           chr2:32541026-32538200 | 20130731
          Length = 443

 Score =  694 bits (1792), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 342/425 (80%), Positives = 366/425 (86%), Gaps = 7/425 (1%)

Query: 153 IIWG-----TCVIVGKCDIEGTIAIDSQDTRGFSLTGSGVSTDIWTSYAARIMVLSIVPF 207
           I+W      +C   GKCDIE +IA+DS DTRGF LTGSGVSTDIWTSYAARIM++S++PF
Sbjct: 21  IVWAFRKYRSCSKSGKCDIEDSIALDSTDTRGFHLTGSGVSTDIWTSYAARIMIISVIPF 80

Query: 208 LIVQLPQALDSTSGRHLAVLIALIVSVCLLISYCLYQIFQPWIQRRKLEYIEHKHVILGF 267
           LIVQLPQ L S SGRHLAVLI  +VSVCLLISYCLYQIFQPWIQRRKLEYI+HKHVI+G 
Sbjct: 81  LIVQLPQILHSNSGRHLAVLIGFVVSVCLLISYCLYQIFQPWIQRRKLEYIKHKHVIVGL 140

Query: 268 LKHLKMRSLGRLLREDGQPDTEVIKKLFTTIDENKDGSLTHGELRALVVGIQFEEIDLDH 327
           L+HLKMRSLGRLL++DGQPD EVIKKLFTTIDENKDG LTHGE+RALVVGIQFEEIDLDH
Sbjct: 141 LRHLKMRSLGRLLKDDGQPDKEVIKKLFTTIDENKDGHLTHGEMRALVVGIQFEEIDLDH 200

Query: 328 DDAVKRIMVDFDTSNNQLIDEEEFVSGVCRWLQRARGARVASGDAGPHTMKFLSDYHTET 387
           DDAV +IM DFDTS NQLIDE EFV+GVCRWL   + +RVASGDAGPHT+KFLSD+HTET
Sbjct: 201 DDAVTKIMADFDTSRNQLIDEGEFVNGVCRWLLATQRSRVASGDAGPHTVKFLSDFHTET 260

Query: 388 KMEHDLLDVGVGGQSEEVAEGVENARWTSTKXXXXXXXXXXXXXXXXXXXVDAVDNFSEA 447
           K EHDLLD  VGGQS+E AEGVENARW S K                   VD VDNFS+A
Sbjct: 261 KREHDLLD--VGGQSDEGAEGVENARWISIKAGLFLLLGSLIAAAFADPLVDVVDNFSDA 318

Query: 448 TTIPAFFISFIFLPLATNSSEAVSAIIFASRDKRQTASLTFSEIYGAVTMNNVLCLSVFL 507
           T+IPAFFISFIFLPLATNSSEAVSAIIFASRDKRQTASLTFSEIYGAVTMNNVLCLSVFL
Sbjct: 319 TSIPAFFISFIFLPLATNSSEAVSAIIFASRDKRQTASLTFSEIYGAVTMNNVLCLSVFL 378

Query: 508 ALVYARGLTWDFSSEVLVIFIVCIVMGVFASFRTVFPLWTSILAILLYPFSLALVYVLDY 567
           ALVY RGLTWDFSSEVLVI IVCIVMG FASFRTVFPLWTSI AILLYPFSLALVYVLDY
Sbjct: 379 ALVYVRGLTWDFSSEVLVILIVCIVMGAFASFRTVFPLWTSITAILLYPFSLALVYVLDY 438

Query: 568 VFGWS 572
            FGWS
Sbjct: 439 QFGWS 443


>Medtr2g078350.1 | calcium-binding EF-hand protein | HC |
           chr2:32631917-32635869 | 20130731
          Length = 509

 Score =  182 bits (463), Expect = 6e-46,   Method: Compositional matrix adjust.
 Identities = 93/252 (36%), Positives = 156/252 (61%), Gaps = 7/252 (2%)

Query: 120 ILVSGLSGSKETAQSQVSVGMGLLAGSTVLLLTIIWGTCVIVGKCDIEGTIAIDSQDTRG 179
           +LV+GLS  KE+AQ   S G+GLLAGS++LLLT++WGTCVIVGK  +             
Sbjct: 1   MLVTGLSSDKESAQEYASTGVGLLAGSSILLLTVVWGTCVIVGKQSLRNDSHSSDTSNSS 60

Query: 180 F-----SLTGSGVSTDIWTSYAARIMVLSIVPFLIVQLPQALD-STSGRHLAVLIALIVS 233
                 +LTG G++ D+ T   ARIM+ S++P LI+Q+P     ST+  ++ ++I+LI++
Sbjct: 61  TGRIKQALTGYGITMDVDTRKMARIMLFSVIPLLIMQIPTLFQFSTTLHNVTLMISLIIA 120

Query: 234 VCLLISYCLYQIFQPWIQRRKLEYIEHKHVILGFLKHLKMRSLGRLLREDGQPDTEVIKK 293
           V  L+SY +YQIF+P I++ +LEYI+H  +IL   + ++ ++L  +L EDG PD + I +
Sbjct: 121 VTFLVSYFIYQIFKPQIEKTRLEYIKHDELILRIFERVEKQTLQTILTEDGTPDVDAINR 180

Query: 294 LFTTIDENKDGSLTHGELRALVVGIQFEEIDLDHDDAVKRIMVDFDTSNNQLIDEEEFVS 353
           L+   +++    L+  ++R L+ G      ++  D+ +  ++  FD   +Q+I +EEFV+
Sbjct: 181 LYHEFNQHGREHLSPSDVRELLFGNNLTSTEI-RDEQITDMLKVFDKDGDQIITKEEFVN 239

Query: 354 GVCRWLQRARGA 365
           GV  +  + + A
Sbjct: 240 GVTEYFNQTKHA 251



 Score =  109 bits (272), Expect = 9e-24,   Method: Compositional matrix adjust.
 Identities = 81/252 (32%), Positives = 116/252 (46%), Gaps = 23/252 (9%)

Query: 242 LYQIF-QPWIQRRKLEYIEHKHVILGFLKHLKMRSLGRLLREDGQPDTEVIKKLFTTIDE 300
           +YQ F +PWI+  + E    +H+I G L H +   +GRLL++DG PD + IK+ F  ID 
Sbjct: 265 MYQSFIKPWIEHTRKERELKEHLISGVLSHAQSDRVGRLLKDDGTPDKDAIKRFFEEIDS 324

Query: 301 NKDGSLTHGELRALVVGIQFEEIDLDHDDAVKRIMVDFDTSNNQLIDEEEFVSGVCRWLQ 360
           N D S++  EL   V  I+F +     ++AV + +   D + +  I E EFV G+   + 
Sbjct: 325 NDDKSVSRSELENAVKKIKFGKT---AEEAVTKFLQVLDVNGDNEISETEFVDGITELIN 381

Query: 361 RARGARVASGDAGPHTMKFLSDYHTETKME-HDLLDVGVGGQSEEVAEGVENARWTSTKX 419
              G   +S           S  H ET     D+  V    Q++ V   +E   +     
Sbjct: 382 SYFGQDASSK----------SPSHHETHQTWEDVEKVMEENQTKGVTAWLEAIAYV---- 427

Query: 420 XXXXXXXXXXXXXXXXXXVDAVDNFSEATTIPAFFISFIFLPLATNSSEAVSAIIFASRD 479
                             + +V  FSEA  I +FFISFI +PLATN  EA SAI  AS  
Sbjct: 428 ----VLGITMLSILAEPLIASVQKFSEAAGISSFFISFILVPLATNFREATSAIKEASHK 483

Query: 480 KRQTASLTFSEI 491
           K    S T  E+
Sbjct: 484 KSSNTSHTMYEV 495


>Medtr2g078250.1 | calcium ion-binding protein, putative | HC |
           chr2:32542664-32542263 | 20130731
          Length = 133

 Score =  171 bits (434), Expect = 1e-42,   Method: Compositional matrix adjust.
 Identities = 90/117 (76%), Positives = 100/117 (85%), Gaps = 3/117 (2%)

Query: 5   SPSHRTLFIFFLLILCGHTHARFFTPLDPVSDGGWSSIIRLPTAEESTCEQTYGFLPCTT 64
           +P    LF+  + ILC HTHARF T  D +SDG  S+++RLPT  +STCEQTYGFLPCTT
Sbjct: 18  TPPFFFLFLGVVAILCSHTHARFST-RDLISDG--SNVLRLPTEADSTCEQTYGFLPCTT 74

Query: 65  TVLGNLFLILIYGFLMFKAATFLSNGSELLLEILGPGIVGGLFLPILGALPDAMLIL 121
           TVLGNLFLIL+YGFLM+ AATFLSNGSELLLEILGPGIVGGLFLPILGALPDAMLIL
Sbjct: 75  TVLGNLFLILVYGFLMYTAATFLSNGSELLLEILGPGIVGGLFLPILGALPDAMLIL 131


>Medtr2g078340.1 | calcium-binding EF-hand protein | HC |
           chr2:32614673-32617960 | 20130731
          Length = 413

 Score = 70.5 bits (171), Expect = 5e-12,   Method: Compositional matrix adjust.
 Identities = 35/122 (28%), Positives = 74/122 (60%), Gaps = 2/122 (1%)

Query: 244 QIFQPWIQRRKLEYIEHKHVILGFLKHLKMRSLGRLLREDGQPDTEVIKKLFTTIDENKD 303
           Q+F+P I++ +LEYI+H  +IL   + ++ ++L ++L EDG P+   I  L+    ++  
Sbjct: 2   QLFKPQIEKTRLEYIKHDDLILRIFQRVEKQTLQKILTEDGTPNVAAINGLYHEFSQH-G 60

Query: 304 GSLTHGELRALVVGIQFEEIDLDHDDAVKRIMVDFDTSNNQLIDEEEFVSGVCRWLQRAR 363
             L+  E++ L++G    + ++  +D +  ++  FD + +Q+I +EEFVSG+  ++ + +
Sbjct: 61  KHLSTSEVKELLLGNNLADTNI-REDQIADMLKAFDKNGDQIITKEEFVSGLTEYINQTK 119

Query: 364 GA 365
            A
Sbjct: 120 HA 121



 Score = 55.5 bits (132), Expect = 1e-07,   Method: Compositional matrix adjust.
 Identities = 33/69 (47%), Positives = 36/69 (52%)

Query: 440 AVDNFSEATTIPAFFISFIFLPLATNSSEAVSAIIFASRDKRQTASLTFSEIYGAVTMNN 499
           +V  FSEA  I +FFISFI +PLATN  EA SAI  AS  K    S T  E Y     N 
Sbjct: 315 SVQKFSEAAGISSFFISFILVPLATNFREATSAIKEASHKKSSNTSHTMYEEYSDGLHNK 374

Query: 500 VLCLSVFLA 508
            L  S   A
Sbjct: 375 RLSKSEHAA 383