Miyakogusa Predicted Gene

Lj0g3v0067399.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0067399.1 Non Characterized Hit- tr|F0ZAP4|F0ZAP4_DICPU
Putative uncharacterized protein OS=Dictyostelium
purp,36.31,1e-18,S5A_REDUCTASE,3-oxo-5-alpha-steroid 4-dehydrogenase,
C-terminal; 3-OXO-5-ALPHA-STEROID 4-DEHYDROGENA,CUFF.3192.1
         (270 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr6g037340.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family p...   414   e-116
Medtr6g043740.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family p...   380   e-106
Medtr6g037330.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family p...   144   6e-35
Medtr6g037370.1 | steroid 5-alpha-reductase DET2-like protein | ...   129   2e-30
Medtr7g066760.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC | c...    84   1e-16
Medtr4g064025.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC | c...    72   5e-13
Medtr5g087590.1 | trans-2-enoyl-CoA reductase | HC | chr5:379718...    64   1e-10
Medtr4g072070.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family p...    60   3e-09

>Medtr6g037340.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family
           protein | HC | chr6:13161597-13166397 | 20130731
          Length = 268

 Score =  414 bits (1064), Expect = e-116,   Method: Compositional matrix adjust.
 Identities = 195/252 (77%), Positives = 223/252 (88%), Gaps = 1/252 (0%)

Query: 19  FVTAMSVLSFVALGNSGFSEIRGNHLNYSKFWNANTGGAAAAQRQVKLSSRTGMLWLYTP 78
           FVTAMS++SFVAL N G SEIRG HLNYSKFWNAN     +  +Q+KLSS+ GML LYTP
Sbjct: 18  FVTAMSIISFVALANYGISEIRGKHLNYSKFWNANNNKTNSV-KQIKLSSKVGMLLLYTP 76

Query: 79  AFLAGAASFWVFPDEGLRSTLVQSAVTLHFLKRVLEVLFVHKYSGSMLLDSAIPITTSYF 138
           AFLAGA SFW+FP+EGLRST++Q A+TLH+ KRV E LFVHKYSGSM+LDSAIPIT SY 
Sbjct: 77  AFLAGAVSFWIFPNEGLRSTILQGALTLHYFKRVFESLFVHKYSGSMILDSAIPITLSYL 136

Query: 139 MSAVTMIYAQHLTRELPEPPVNLMYPGIVLFLVGIIGNFYHHYLLSKLRGKGEKEYKIPK 198
           MS +TMIY QHLT++LPEP +NL+YPGIVLFLVGIIGNFYHH+LLSKLRGKGEKEYKIPK
Sbjct: 137 MSTITMIYGQHLTKDLPEPQINLIYPGIVLFLVGIIGNFYHHFLLSKLRGKGEKEYKIPK 196

Query: 199 GGLFDLVVCPHYLFEIIVFYGVSFMSQTLYSVSFAVGTTFYLLGRSYATRKWYLSKFEDF 258
           GGLF +V+CPHY+FEII FYGV F+SQTLYS+ FA+GTTFYLLGRS+ATR WYLSKFEDF
Sbjct: 197 GGLFGIVICPHYMFEIIDFYGVFFISQTLYSLIFAIGTTFYLLGRSHATRNWYLSKFEDF 256

Query: 259 PNHVKAVIPFLF 270
           P +VKAVIPF+F
Sbjct: 257 PKNVKAVIPFVF 268


>Medtr6g043740.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family
           protein | HC | chr6:13210802-13213205 | 20130731
          Length = 270

 Score =  380 bits (977), Expect = e-106,   Method: Compositional matrix adjust.
 Identities = 185/253 (73%), Positives = 217/253 (85%), Gaps = 1/253 (0%)

Query: 19  FVTAMSVLSFVALGNSGFSEIRGNHLNYSKFWNA-NTGGAAAAQRQVKLSSRTGMLWLYT 77
           F+T MSV++ V+L N+G +E+RG HLNYSKFWN  N  G    ++ +KLSS++GML LYT
Sbjct: 18  FITTMSVITCVSLANAGINEVRGKHLNYSKFWNVENNNGDKKKKKMIKLSSKSGMLLLYT 77

Query: 78  PAFLAGAASFWVFPDEGLRSTLVQSAVTLHFLKRVLEVLFVHKYSGSMLLDSAIPITTSY 137
           PAF+AGAASF VFPD+G RS ++Q AVT HF KRV EVLFVHKYSGSM L++AIPIT SY
Sbjct: 78  PAFVAGAASFLVFPDDGFRSLVLQGAVTFHFFKRVFEVLFVHKYSGSMALETAIPITLSY 137

Query: 138 FMSAVTMIYAQHLTRELPEPPVNLMYPGIVLFLVGIIGNFYHHYLLSKLRGKGEKEYKIP 197
           F+S+ T+IYAQHLT  LPEP ++L+YPGI LFLVG+ GNFYHHYLLSKLRGKGEKEYKIP
Sbjct: 138 FLSSATLIYAQHLTSNLPEPSIDLLYPGIALFLVGVTGNFYHHYLLSKLRGKGEKEYKIP 197

Query: 198 KGGLFDLVVCPHYLFEIIVFYGVSFMSQTLYSVSFAVGTTFYLLGRSYATRKWYLSKFED 257
           KGGLFD V+CPHYLFEII FYG SF+SQTLYS SFA+GTTFYLLGRSYATR+WYLSKF+D
Sbjct: 198 KGGLFDFVICPHYLFEIIGFYGFSFISQTLYSFSFAIGTTFYLLGRSYATREWYLSKFDD 257

Query: 258 FPNHVKAVIPFLF 270
           FP +VKA+IPF F
Sbjct: 258 FPKNVKAIIPFWF 270


>Medtr6g037330.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family
           protein | HC | chr6:13156844-13158910 | 20130731
          Length = 128

 Score =  144 bits (364), Expect = 6e-35,   Method: Compositional matrix adjust.
 Identities = 68/98 (69%), Positives = 81/98 (82%), Gaps = 1/98 (1%)

Query: 19  FVTAMSVLSFVALGNSGFSEIRGNHLNYSKFWNANTGGAAAAQRQVKLSSRTGMLWLYTP 78
           FVTAMS++SFVAL N G SEIRG HLNYSKFWNAN     +  +Q+KLSS+ GML LYTP
Sbjct: 18  FVTAMSIISFVALANYGISEIRGKHLNYSKFWNANNNKTNSV-KQIKLSSKVGMLLLYTP 76

Query: 79  AFLAGAASFWVFPDEGLRSTLVQSAVTLHFLKRVLEVL 116
           AFLAGA SFW+FP+EGLRST++Q A+TLH+ KRV EV+
Sbjct: 77  AFLAGAVSFWIFPNEGLRSTILQGALTLHYFKRVFEVI 114


>Medtr6g037370.1 | steroid 5-alpha-reductase DET2-like protein | HC
           | chr6:13184734-13185516 | 20130731
          Length = 116

 Score =  129 bits (325), Expect = 2e-30,   Method: Compositional matrix adjust.
 Identities = 62/97 (63%), Positives = 75/97 (77%), Gaps = 1/97 (1%)

Query: 23  MSVLSFVALGNSGFSEIRGNHLNYSKFWNANTGGAAAAQRQVKLSSRTGMLWLYTPAFLA 82
           MS++SFVA  + G SEIRG HLNYSKFWNAN     +  +Q+KLSS+ GML LYTPAFLA
Sbjct: 1   MSIISFVASSSYGISEIRGKHLNYSKFWNANNNKTNSG-KQIKLSSKVGMLLLYTPAFLA 59

Query: 83  GAASFWVFPDEGLRSTLVQSAVTLHFLKRVLEVLFVH 119
           GA SFW+FP+EGLRST++Q A+TLH+ KRV E    H
Sbjct: 60  GAVSFWIFPNEGLRSTILQGALTLHYFKRVFESHLDH 96


>Medtr7g066760.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC |
           chr7:24346408-24345279 | 20130731
          Length = 265

 Score = 84.3 bits (207), Expect = 1e-16,   Method: Compositional matrix adjust.
 Identities = 46/107 (42%), Positives = 68/107 (63%), Gaps = 2/107 (1%)

Query: 165 GIVLFLVGIIGNFYHHYLLSKLRGKGEKEYKIPKGGLFDLVVCPHYLFEIIVFYGVSFMS 224
           G+ +F VG++ N +    L +L+G+G K Y IPKGGLF+ V CP+Y  EI+ ++G + M+
Sbjct: 160 GVFVFFVGMMINVWSDTELLRLKGEG-KGYVIPKGGLFEFVSCPNYFGEIVEWFGWALMT 218

Query: 225 QTLYSVSFAVGTTFYLLGRSYATRKWYLSKF-EDFPNHVKAVIPFLF 270
            +   + F + T   L  R+ A  +WYL KF ED+P   KAVIPFL+
Sbjct: 219 WSWAGLGFFIYTFANLGPRARANHQWYLEKFGEDYPKKRKAVIPFLY 265


>Medtr4g064025.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC |
           chr4:23886573-23887358 | 20130731
          Length = 261

 Score = 72.0 bits (175), Expect = 5e-13,   Method: Compositional matrix adjust.
 Identities = 39/89 (43%), Positives = 58/89 (65%), Gaps = 2/89 (2%)

Query: 183 LSKLRGKGEKEYKIPKGGLFDLVVCPHYLFEIIVFYGVSFMSQTLYSVSFAVGTTFYLLG 242
           L +L+ +G K Y IPKGGLF++V CP+Y  EI+ ++G + M+ +   + F + T   L  
Sbjct: 174 LLRLKSEG-KGYLIPKGGLFEVVSCPNYFGEIVEWFGWALMTWSWAGLGFFLYTFANLGP 232

Query: 243 RSYATRKWYLSKF-EDFPNHVKAVIPFLF 270
           R+ A  +WYL KF ED+P   KAVIP+L+
Sbjct: 233 RARANHQWYLEKFGEDYPKKRKAVIPYLY 261


>Medtr5g087590.1 | trans-2-enoyl-CoA reductase | HC |
           chr5:37971894-37967988 | 20130731
          Length = 310

 Score = 64.3 bits (155), Expect = 1e-10,   Method: Compositional matrix adjust.
 Identities = 42/125 (33%), Positives = 67/125 (53%), Gaps = 3/125 (2%)

Query: 105 TLHFLKRVLEVLFVHKYSGSMLLDSAIPITTSYFMSAVTMIYAQHLTRELPEPPVNL-MY 163
             H+ KR+LE  FVH++S +    S +    +Y+ +    I A ++   L  P  +L M 
Sbjct: 139 CFHYAKRILETFFVHRFSHATSPLSNVFRNCAYYWTFGCYI-AYYVNHPLYTPVSDLQMK 197

Query: 164 PGIVLFLVGIIGNFYHHYLLSKLRGK-GEKEYKIPKGGLFDLVVCPHYLFEIIVFYGVSF 222
            G    ++  + NFY H +L  LRG  GE  Y+IP+G LF++V C +Y  EI  + G + 
Sbjct: 198 IGFGFGILCQVSNFYCHIILRNLRGPAGEGGYQIPRGFLFNIVTCANYTTEIYQWLGFNI 257

Query: 223 MSQTL 227
            +QT+
Sbjct: 258 ATQTI 262


>Medtr4g072070.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family
           protein | HC | chr4:27340126-27335902 | 20130731
          Length = 484

 Score = 59.7 bits (143), Expect = 3e-09,   Method: Compositional matrix adjust.
 Identities = 39/111 (35%), Positives = 60/111 (54%), Gaps = 7/111 (6%)

Query: 165 GIVLFLVGIIGNFYHHYLLSKLR-GKGEKEYKIPKGGLFDLVVCPHYLFEIIVFYGVSFM 223
           G  +F  G I  +  H +L  LR  +   EY IP+G  F++V  PHYL EI+++   SF+
Sbjct: 376 GAAIFSWGWIHQYRCHKILGSLRESRQADEYVIPRGDWFEIVSSPHYLSEIVIY--ASFV 433

Query: 224 SQTLYS-VSFAVGTTFYLLGRSYA---TRKWYLSKFEDFPNHVKAVIPFLF 270
             T  S ++  +   F +   ++A   T  WY  KFED+P+   A+IPF+ 
Sbjct: 434 VATGGSNLTIWLLFAFVVANLAFAAVETHSWYRRKFEDYPSSRFAIIPFIL 484