Miyakogusa Predicted Gene

Lj0g3v0063869.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0063869.1 Non Characterized Hit- tr|D7TF60|D7TF60_VITVI
Putative uncharacterized protein OS=Vitis vinifera
GN=,83.41,0,AA_TRNA_LIGASE_I,Aminoacyl-tRNA synthetase, class I,
conserved site; TRNASYNTHVAL,Valine-tRNA ligase,CUFF.2934.1
         (426 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr6g027620.1 | valyl-tRNA synthetase/valine-tRNA ligase | HC ...   720   0.0  
Medtr1g101620.2 | valyl-tRNA synthetase/valine-tRNA ligase | HC ...   310   2e-84
Medtr1g101620.1 | valyl-tRNA synthetase/valine-tRNA ligase | HC ...   310   2e-84
Medtr2g436760.1 | isoleucine-tRNA ligase | HC | chr2:14336824-14...   129   7e-30
Medtr4g076850.1 | isoleucine-tRNA ligase | HC | chr4:29387451-29...    80   4e-15
Medtr4g132500.2 | leucyl-tRNA synthetase | HC | chr4:55397369-55...    67   5e-11
Medtr4g132500.1 | leucyl-tRNA synthetase | HC | chr4:55397313-55...    67   5e-11

>Medtr6g027620.1 | valyl-tRNA synthetase/valine-tRNA ligase | HC |
           chr6:9605327-9589286 | 20130731
          Length = 980

 Score =  720 bits (1859), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 353/411 (85%), Positives = 365/411 (88%), Gaps = 11/411 (2%)

Query: 27  LNPLLFYTRRRAIPCC---------RRFTTTRLLAVAAS--ENGVFTSPEIAKSFDFSAE 75
            NPLLFYTRR                RF T  L  VAA+   NGVFTSPEIAK+FDF+AE
Sbjct: 22  FNPLLFYTRRSLSSHSHSHSHSRSRNRFPTRLLTTVAAASENNGVFTSPEIAKTFDFAAE 81

Query: 76  ERIYNWWESQGYFKPTFDRGSDPFVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMKGR 135
           ERIYNWWESQGYFKP FDRGSDPFVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMKGR
Sbjct: 82  ERIYNWWESQGYFKPNFDRGSDPFVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMKGR 141

Query: 136 PTLWLPGTDHAGIATQLVVERMLASEGIKRVEMTRDEFTTRVWQWKEKYGGTITNQIKRL 195
           PTLWLPGTDHAGIATQLVVERMLASEGIKRVE+ RDEFT +VWQWKEKYGGTITNQIKRL
Sbjct: 142 PTLWLPGTDHAGIATQLVVERMLASEGIKRVEIGRDEFTRKVWQWKEKYGGTITNQIKRL 201

Query: 196 GASCDWSREHFTLDEQLSHAVVEAFVRLHEKGLIYQGSYMVNWSPTLQTAVSDLEVEYSE 255
           GASCDWSREHFTLDEQLS AVVEAFVRLHEKGLIYQGSYMVNWSPTLQTAVSDLEVEYSE
Sbjct: 202 GASCDWSREHFTLDEQLSQAVVEAFVRLHEKGLIYQGSYMVNWSPTLQTAVSDLEVEYSE 261

Query: 256 ESGYLYYIRYRIAGGSRSDYLTVATTRPETLFGDVALAVNPHDDRYSKYIGQMAIVPQTF 315
           ESGY+Y+IRYR+AGGSR D+LTVATTRPETLFGDVALAVNP D+RYSKYIGQMAIVP TF
Sbjct: 262 ESGYMYHIRYRVAGGSRDDWLTVATTRPETLFGDVALAVNPKDERYSKYIGQMAIVPLTF 321

Query: 316 GRHVPIIYDKHVDKEFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGTLNDVAGLYS 375
           GRHVPII DK+VDKEFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGTLN+VAGLYS
Sbjct: 322 GRHVPIIADKYVDKEFGTGVLKISPGHDHNDYLLARKLGLPILNVMNKDGTLNEVAGLYS 381

Query: 376 GLDRFXXXXXXXXXXXXTGLAVKKEPHTLRVPRSQRGGEVIEPLVSKQWFV 426
           GLDRF            TGL VKKEPHTLRVPRSQRGGEVIEPLVSKQWFV
Sbjct: 382 GLDRFEVRKKMWAELEETGLGVKKEPHTLRVPRSQRGGEVIEPLVSKQWFV 432


>Medtr1g101620.2 | valyl-tRNA synthetase/valine-tRNA ligase | HC |
           chr1:45900200-45890846 | 20130731
          Length = 1053

 Score =  310 bits (793), Expect = 2e-84,   Method: Compositional matrix adjust.
 Identities = 166/384 (43%), Positives = 231/384 (60%), Gaps = 23/384 (5%)

Query: 63  SPEIAKSFDFSAEERI-YNWWESQGYFKPTFDRGSDPFVISMPPPNVTGSLHMGHAMFVT 121
           S ++A+ ++ +A E+  Y WWE   YF         PFVI +PPPNVTG+LH+GHA+   
Sbjct: 95  SRQLAQKYNPTAVEKSWYEWWEKSKYFVADASSKKPPFVIVLPPPNVTGALHIGHALTAA 154

Query: 122 LEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASE-GIKRVEMTRDEFTTRVWQW 180
           +ED M+R+ RM G  TLW+PG DHAGIATQ+VVE+ +  E  + R ++ R++F + VW+W
Sbjct: 155 IEDTMIRWRRMSGYNTLWVPGMDHAGIATQVVVEKKIMRERKLTRHDLGREKFISEVWEW 214

Query: 181 KEKYGGTITNQIKRLGASCDWSREHFTLDEQLSHAVVEAFVRLHEKGLIYQGSYMVNWSP 240
           K KYGGTI  Q++RLGAS DWSRE FT+D++ S  V EAFVRL++   IY+   +VNW  
Sbjct: 215 KNKYGGTILQQLRRLGASLDWSRECFTMDDKRSKGVTEAFVRLYKLDRIYRDVRLVNWDC 274

Query: 241 TLQTAVSDLEVEYSE----------------ESGYLYYIRYRIAGGSRSDYLTVATTRPE 284
            L+TAVSD+EV+Y E                E G L    Y + G      + VATTR E
Sbjct: 275 VLRTAVSDIEVDYIEIKERTLLKVPGYDRPVEFGVLKKFAYPLEGNLGE--IVVATTRIE 332

Query: 285 TLFGDVALAVNPHDDRYSKYIGQMAIVPQTFGRHVPIIYDK-HVDKEFGTGVLKISPGHD 343
           T+ GD A+AV+P DDRYS++ G+ A+ P   GR +PII D   VD +FGTG +KI+P HD
Sbjct: 333 TMLGDTAIAVHPTDDRYSQFHGKYAVHPFN-GRKIPIICDAILVDPKFGTGAVKITPAHD 391

Query: 344 HNDYLLARKLGLPILNVMNKDGTLNDVAGL-YSGLDRFXXXXXXXXXXXXTGLAVKKEPH 402
            ND+ + ++  L  +NV   DG +N   G  +  + RF             GL    E +
Sbjct: 392 PNDFEVGKRHKLEFINVFTDDGKVNSNGGSEFLDMPRFKAREAITEALQKKGLYRGSENN 451

Query: 403 TLRVPRSQRGGEVIEPLVSKQWFV 426
            +R+    R  +VIEP++  QW+V
Sbjct: 452 EMRLGVCSRSNDVIEPMIKPQWYV 475


>Medtr1g101620.1 | valyl-tRNA synthetase/valine-tRNA ligase | HC |
           chr1:45900200-45890846 | 20130731
          Length = 1053

 Score =  310 bits (793), Expect = 2e-84,   Method: Compositional matrix adjust.
 Identities = 166/384 (43%), Positives = 231/384 (60%), Gaps = 23/384 (5%)

Query: 63  SPEIAKSFDFSAEERI-YNWWESQGYFKPTFDRGSDPFVISMPPPNVTGSLHMGHAMFVT 121
           S ++A+ ++ +A E+  Y WWE   YF         PFVI +PPPNVTG+LH+GHA+   
Sbjct: 95  SRQLAQKYNPTAVEKSWYEWWEKSKYFVADASSKKPPFVIVLPPPNVTGALHIGHALTAA 154

Query: 122 LEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASE-GIKRVEMTRDEFTTRVWQW 180
           +ED M+R+ RM G  TLW+PG DHAGIATQ+VVE+ +  E  + R ++ R++F + VW+W
Sbjct: 155 IEDTMIRWRRMSGYNTLWVPGMDHAGIATQVVVEKKIMRERKLTRHDLGREKFISEVWEW 214

Query: 181 KEKYGGTITNQIKRLGASCDWSREHFTLDEQLSHAVVEAFVRLHEKGLIYQGSYMVNWSP 240
           K KYGGTI  Q++RLGAS DWSRE FT+D++ S  V EAFVRL++   IY+   +VNW  
Sbjct: 215 KNKYGGTILQQLRRLGASLDWSRECFTMDDKRSKGVTEAFVRLYKLDRIYRDVRLVNWDC 274

Query: 241 TLQTAVSDLEVEYSE----------------ESGYLYYIRYRIAGGSRSDYLTVATTRPE 284
            L+TAVSD+EV+Y E                E G L    Y + G      + VATTR E
Sbjct: 275 VLRTAVSDIEVDYIEIKERTLLKVPGYDRPVEFGVLKKFAYPLEGNLGE--IVVATTRIE 332

Query: 285 TLFGDVALAVNPHDDRYSKYIGQMAIVPQTFGRHVPIIYDK-HVDKEFGTGVLKISPGHD 343
           T+ GD A+AV+P DDRYS++ G+ A+ P   GR +PII D   VD +FGTG +KI+P HD
Sbjct: 333 TMLGDTAIAVHPTDDRYSQFHGKYAVHPFN-GRKIPIICDAILVDPKFGTGAVKITPAHD 391

Query: 344 HNDYLLARKLGLPILNVMNKDGTLNDVAGL-YSGLDRFXXXXXXXXXXXXTGLAVKKEPH 402
            ND+ + ++  L  +NV   DG +N   G  +  + RF             GL    E +
Sbjct: 392 PNDFEVGKRHKLEFINVFTDDGKVNSNGGSEFLDMPRFKAREAITEALQKKGLYRGSENN 451

Query: 403 TLRVPRSQRGGEVIEPLVSKQWFV 426
            +R+    R  +VIEP++  QW+V
Sbjct: 452 EMRLGVCSRSNDVIEPMIKPQWYV 475


>Medtr2g436760.1 | isoleucine-tRNA ligase | HC |
           chr2:14336824-14349112 | 20130731
          Length = 1080

 Score =  129 bits (323), Expect = 7e-30,   Method: Compositional matrix adjust.
 Identities = 114/426 (26%), Positives = 181/426 (42%), Gaps = 77/426 (18%)

Query: 75  EERIYNWWESQGYFKPTFDRGSD-PFVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMK 133
           E  I   WE    FK   D+ S   F++   PP   G LH+GHA+   L+DI+ RY  ++
Sbjct: 110 EPEIQKIWEDNQVFKRVADKNSGGNFILHDGPPYANGDLHIGHALNKILKDIINRYKLLQ 169

Query: 134 GRPTLWLPGTDHAGIATQLVVERMLASEGIKRVEMTRDEFTTRVWQWKEKYGGTITNQIK 193
                ++PG D  G+  +L V + L  E   R  +T  +   +  ++ +    T  +  K
Sbjct: 170 NYKVHFVPGWDCHGLPIELKVLQSLDKEA--RNNLTPLKLRAKAAKFAKDTVKTQMSSFK 227

Query: 194 RLGASCDWSREHFTLDEQLSHAVVEAFVRLHEKGLIYQGSYMVNWSPTLQTAVSDLEVEY 253
           R G   DW+  + TLD +   A +E F ++  KG IY+G   V+WSP+ +TA+++ E+EY
Sbjct: 228 RFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEY 287

Query: 254 SEE--SGYLYYIRYRIAGGSR--SDYL--------TVATTRPETLFGDVALAV------- 294
            E   S  +Y I +RIA      SD L         + TT P T+  + A+AV       
Sbjct: 288 PEGHVSKSIYAI-FRIASAPLMPSDLLQEFPNLCLAIWTTTPWTIPANAAVAVNSKLEYD 346

Query: 295 -------NPHD----DRYSKYIGQM-------------AIVPQ----------------- 313
                  N HD    D   K +G +              +VP                  
Sbjct: 347 VIEVEPLNEHDSSSGDTKKKRLGIVLKNEKKLFLIVASELVPTLEAKWGVKLIFKRRLLG 406

Query: 314 -----------TFGRHVPIIYD-KHVDKEFGTGVLKISPGHDHNDYLLARKLGLPILNVM 361
                         R  P++    ++  E GTG++  +PGH   DY+  +K GLPIL+ +
Sbjct: 407 SDLENYRYIHPVDNRECPVVVGGDYITTETGTGLVHTAPGHGQEDYVTGQKYGLPILSPV 466

Query: 362 NKDGTLNDVAGLYSGLDRFXXXXXXXXXXXXTGLA-VKKEPHTLRVPRSQRGGEVIEPLV 420
           + +G   + AG +SGLD                L+ + +E +  + P   R  +      
Sbjct: 467 DDNGIFTEEAGQFSGLDVLGEGNTAVVKYLDENLSLIMEESYQHKYPYDWRTKKPTIFRA 526

Query: 421 SKQWFV 426
           ++QWF 
Sbjct: 527 TEQWFA 532


>Medtr4g076850.1 | isoleucine-tRNA ligase | HC |
           chr4:29387451-29405277 | 20130731
          Length = 1179

 Score = 80.1 bits (196), Expect = 4e-15,   Method: Compositional matrix adjust.
 Identities = 71/267 (26%), Positives = 121/267 (45%), Gaps = 23/267 (8%)

Query: 65  EIAKSFDFS---AEERIYNWWESQGYFKPTFDRGSDP--FVISMPPPNVTGSLHMGHAMF 119
           ++ +S DF+    EE I ++W +   F     R  D   ++    PP  TG  H GH + 
Sbjct: 3   DVCESKDFAFAKQEEAILSFWSNIDAFHTQLSRTKDKPEYIFYDGPPFATGLPHYGHILA 62

Query: 120 VTLEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVEMTRDEFTTRVWQ 179
            T++DI+ RY  M G       G D  G+  +  +++ L   GIK+ E   D     +  
Sbjct: 63  GTIKDIVTRYQSMTGHHVTRRFGWDCHGLPVENEIDKKL---GIKKRE---DVLKLGIGV 116

Query: 180 WKEKYGGTIT-------NQIKRLGASCDWSREHFTLDEQLSHAVVEAFVRLHEKGLIYQG 232
           + E+    +T       N I R G   D+  ++ T+D     +V   F +L+ K L+Y+G
Sbjct: 117 YNEECRSIVTRYVEEWENVITRTGRWIDFKNDYKTMDLNFMESVWWVFGQLYAKDLVYKG 176

Query: 233 SYMVNWSPTLQTAVSDLEV--EYSEESGYLYYIRYRIAGGSRSDYLTVATTRPETLFGDV 290
             ++ +S   +T +S+ E    Y + S    Y+ + + G          TT P TL  ++
Sbjct: 177 FKVMPYSTGCKTPLSNFEAGQNYKDVSDPEVYMAFPVLGDQHGASFVAWTTTPWTLPSNL 236

Query: 291 ALAVNPHDDRY---SKYIGQMAIVPQT 314
           AL VN +       +KY G++ IV ++
Sbjct: 237 ALCVNANFTYLKVRNKYTGKVYIVAES 263


>Medtr4g132500.2 | leucyl-tRNA synthetase | HC |
           chr4:55397369-55386514 | 20130731
          Length = 970

 Score = 66.6 bits (161), Expect = 5e-11,   Method: Compositional matrix adjust.
 Identities = 58/228 (25%), Positives = 97/228 (42%), Gaps = 28/228 (12%)

Query: 35  RRRAIPCCRRFTTTRLLAVAASENGVFTSPE----IAKSFDF-SAEERIYNWWESQGYFK 89
           RR +    R  + TR L  +++ +      E    + +++ F   E +   +W+    F+
Sbjct: 41  RRTSFSTLRFRSFTRRLRNSSTNDVQLNETEQKKPVNRAYPFHEIEPKWQRFWDEFCTFR 100

Query: 90  PTFDRGSDP-----FVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMKGRPTLWLPGTD 144
              D   D      +++ M P      LH+GH +  T  DI+ R+ RM+G   L   G D
Sbjct: 101 TPDDDDIDTSKPKYYILDMFPYPSGAGLHVGHPLGYTATDILARFKRMQGYNVLHPMGWD 160

Query: 145 HAGI-ATQLVVERMLASEGIKRVEMTRDEFTTRVWQWKEKYGGTITNQIKRLGASCDWSR 203
             G+ A Q  ++     +      + R                  T+Q+K LG S DW R
Sbjct: 161 AFGLPAEQYAIQTGTHPKLTTVTNINR-----------------FTSQLKSLGFSYDWDR 203

Query: 204 EHFTLDEQLSHAVVEAFVRLHEKGLIYQGSYMVNWSPTLQTAVSDLEV 251
           E  T++ +        F++L ++GL YQ    VNW P L T +++ EV
Sbjct: 204 EISTIEPEYYKWTQWIFLQLLKRGLAYQAEVPVNWCPALGTVLANEEV 251


>Medtr4g132500.1 | leucyl-tRNA synthetase | HC |
           chr4:55397313-55386514 | 20130731
          Length = 970

 Score = 66.6 bits (161), Expect = 5e-11,   Method: Compositional matrix adjust.
 Identities = 58/228 (25%), Positives = 97/228 (42%), Gaps = 28/228 (12%)

Query: 35  RRRAIPCCRRFTTTRLLAVAASENGVFTSPE----IAKSFDF-SAEERIYNWWESQGYFK 89
           RR +    R  + TR L  +++ +      E    + +++ F   E +   +W+    F+
Sbjct: 41  RRTSFSTLRFRSFTRRLRNSSTNDVQLNETEQKKPVNRAYPFHEIEPKWQRFWDEFCTFR 100

Query: 90  PTFDRGSDP-----FVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMKGRPTLWLPGTD 144
              D   D      +++ M P      LH+GH +  T  DI+ R+ RM+G   L   G D
Sbjct: 101 TPDDDDIDTSKPKYYILDMFPYPSGAGLHVGHPLGYTATDILARFKRMQGYNVLHPMGWD 160

Query: 145 HAGI-ATQLVVERMLASEGIKRVEMTRDEFTTRVWQWKEKYGGTITNQIKRLGASCDWSR 203
             G+ A Q  ++     +      + R                  T+Q+K LG S DW R
Sbjct: 161 AFGLPAEQYAIQTGTHPKLTTVTNINR-----------------FTSQLKSLGFSYDWDR 203

Query: 204 EHFTLDEQLSHAVVEAFVRLHEKGLIYQGSYMVNWSPTLQTAVSDLEV 251
           E  T++ +        F++L ++GL YQ    VNW P L T +++ EV
Sbjct: 204 EISTIEPEYYKWTQWIFLQLLKRGLAYQAEVPVNWCPALGTVLANEEV 251