Miyakogusa Predicted Gene

Lj6g3v2275270.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj6g3v2275270.1 Non Chatacterized Hit- tr|I1LTB9|I1LTB9_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.49521
PE,90.43,0,membrane-attack complex / perforin,Membrane attack complex
component/perforin (MACPF) domain; seg,NU,CUFF.61026.1
         (606 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G24290.2 | Symbols:  | MAC/Perforin domain-containing protein...   910   0.0  
AT1G28380.1 | Symbols: NSL1 | MAC/Perforin domain-containing pro...   592   e-169
AT1G14780.1 | Symbols:  | MAC/Perforin domain-containing protein...   476   e-134
AT4G24290.1 | Symbols:  | MAC/Perforin domain-containing protein...   456   e-128
AT1G29690.1 | Symbols: CAD1 | MAC/Perforin domain-containing pro...   449   e-126

>AT4G24290.2 | Symbols:  | MAC/Perforin domain-containing protein |
           chr4:12594856-12597815 FORWARD LENGTH=606
          Length = 606

 Score =  910 bits (2351), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 439/599 (73%), Positives = 512/599 (85%), Gaps = 2/599 (0%)

Query: 1   MALKDPAHKXXXXXXXXXXXXYDISADLRLKYCKGDSIDSRLIEIDEHDVR-DLVLPGGV 59
           MAL+ PA K            YD++ DLRLKYCKG S DSRL++I E D   ++VLPGG+
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 60  SISNVSKSIKCDKGERTRFRSDVLSFQQMSEQFNQELSLTGKIPSGLFNSMFEFSGSWQK 119
           SI NVSKSIKCDKGER RFRSD+L FQQM+EQFNQELSL GKIPSGLFN+MFEFS  WQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 120 DAANTKALAFDGVLITLYTVALEKSQMVLCDHVKKAVPASWDPPALSRFIETFGTHIIVG 179
           DAA TK LAFDGV I+LY+VAL+KSQ++L +HVK+AVP++WDP AL+RFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 180 MKMGGKDVIYLKQQHSSALQPADVQKRLKEMADRRFMDANGQYSVASDQVFPNDKFGVRE 239
           +KMGGKDVIY KQQHSS LQP D+QKRLKE+AD+RF++A+  ++  S++V  + K   +E
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE 240

Query: 240 QRLTFANISPSSSYSHKEDIVSIYKRRGGSDDRNLSHHEWLQTVQVEPDVISMSFIPITS 299
           QRL FA+ S   SY++KED V + KRRGG+D+RNL H+EWLQTVQ+EPDVISMSFIPITS
Sbjct: 241 QRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 300

Query: 300 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQWKQRSSAS 359
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQ KQ+S AS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCAS 360

Query: 360 LQFSFMGPKLYVNTTLVDVGKRPVTGLRLYLEGKKSNRLAIHLQHLSSLPKIFQLVDDPN 419
           LQFSF GPKLYVNTT VDVGKRP+TG+RLYLEG++SNRLAIHLQHLSSLPKI+QL DD N
Sbjct: 361 LQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLN 420

Query: 420 ENFRRKSYDRRFYEKVQWKNFSHVCTAPVESDEDLSIVTGAQLQVENYGIKNILFLRLRF 479
            + R++S+DRR+YEKV WKN+SHVCT PVESD+DLS+VTGAQL VE++G KN+LFLRL F
Sbjct: 421 RSIRQESHDRRYYEKVNWKNYSHVCTEPVESDDDLSVVTGAQLHVESHGFKNVLFLRLCF 480

Query: 480 SSVLGAKEVKHPEWDGSPGLGAKSGLISTLISQHFTATFQKPPPRAADVNINSAVYPGGP 539
           S V+GA  VK+ EWD + G   KSGLISTLIS HFTA  QKPPPR ADVNINSA+YPGGP
Sbjct: 481 SRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAA-QKPPPRPADVNINSAIYPGGP 539

Query: 540 PVPVQAPKLLKFVDTTEMSRGPQENPGYWVVSGARLVVEKGRVSLRVKYSLLTMVLPDE 598
           PVP QAPKLLKFVDT+EM+RGPQE+PGYWVVSGARL+VEKG++SL+VKYSL T +L DE
Sbjct: 540 PVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSLFTPILGDE 598


>AT1G28380.1 | Symbols: NSL1 | MAC/Perforin domain-containing
           protein | chr1:9963696-9966060 FORWARD LENGTH=612
          Length = 612

 Score =  592 bits (1525), Expect = e-169,   Method: Compositional matrix adjust.
 Identities = 311/606 (51%), Positives = 403/606 (66%), Gaps = 22/606 (3%)

Query: 7   AHKXXXXXXXXXXXXYDISADLRLKYCKGDSIDSRLIEIDEHDVRDLVLPGGVSISNVSK 66
           AH             YD+ +D+R   CK     SRL+EID    RDL+ PGG+ ++NVS 
Sbjct: 11  AHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSS 70

Query: 67  SIKCDKGERTRFRSDVLSFQQMSEQFNQELSLTGKIPSGLFNSMFEFSGSWQKDAANTKA 126
           SIKCDKGERTR RSD+LSF QMSE+FNQ++ L+GKIPSG+FN+MF FS  W KDA++ K 
Sbjct: 71  SIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKT 130

Query: 127 LAFDGVLITLYTVALEKSQMVLCDHVKKAVPASWDPPALSRFIETFGTHIIVGMKMGGKD 186
           LA+DG  I+LY+V + + Q+ L D VK+ VP+SWD  AL+ FIE +GTH++VG+ MGGKD
Sbjct: 131 LAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKD 190

Query: 187 VIYLKQQHSSALQPADVQKRLKEMADRRF----MDANGQYSVASDQVFPNDKFGVREQRL 242
           VI++KQ   S  +P ++QK LK   D RF    +++    SV S +  P ++  ++    
Sbjct: 191 VIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGK--PKEENLLQWGLQ 248

Query: 243 TFANISPSSSYSH--KEDIVSIYKRRGGSDDRNLSHHEWLQTVQVEPDVISMSFIPITSL 300
            F     S+   H   E+I+ +  RRGG D    SH  WL TV   P+VISM F+PITSL
Sbjct: 249 PFGTSVSSAVVMHTKNEEIMRVCIRRGGVD-LGQSHERWLSTVSQAPNVISMCFVPITSL 307

Query: 301 LNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQWKQRSSASL 360
           L+G+PG+GFLSHA+NLYLRYKPPIEELHQFLEFQLPRQWAPV+ +LPLG +  ++SS SL
Sbjct: 308 LSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSL 367

Query: 361 QFSFMGPKLYVNTTLVDVGKRPVTGLRLYLEGKKSNRLAIHLQHLSSLPKIFQLVDDPNE 420
           QFS MGPKLYVNT+ VD G+RPVTGLR +LEGKK N LAIHLQHLS+ P    L  D   
Sbjct: 368 QFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTY 427

Query: 421 NFRRKSYDRRFYEKVQWKNFSHVCTAPVE-----SDEDLSIVTGAQLQVENYGIKNILFL 475
               +  ++ +Y  V+W  FSHVCT PV+     SD+  SIVT A L+V+  G++ +LFL
Sbjct: 428 EPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGARSDDTASIVTKAWLEVKGMGMRKVLFL 487

Query: 476 RLRFSSVLGAKEV-KHPEWDGSPGLGAKSGLISTLISQHFTATFQKPPPRA---ADVNIN 531
           RL FS  L A  V +   WD       KSG+ S +IS   +      P      + ++IN
Sbjct: 488 RLGFS--LDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKIDIN 544

Query: 532 SAVYPGGPPVPVQAPKLLKFVDTTEMSRGPQENPGYWVVSGARLVVEKGRVSLRVKYSLL 591
           SAVYP GP  PV+ PKLL  VDT E+ RGP+E PGYWVV+GA+L VE G++S++ KYSLL
Sbjct: 545 SAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 603

Query: 592 TMVLPD 597
           T++  D
Sbjct: 604 TVISED 609


>AT1G14780.1 | Symbols:  | MAC/Perforin domain-containing protein |
           chr1:5091020-5093873 FORWARD LENGTH=627
          Length = 627

 Score =  476 bits (1224), Expect = e-134,   Method: Compositional matrix adjust.
 Identities = 273/608 (44%), Positives = 380/608 (62%), Gaps = 47/608 (7%)

Query: 22  YDISADLRLKYCK---GDSIDSRLIEIDEHDVRDLVLPGGVSISNVSKSIKCDKGERTRF 78
           +D++AD RLKYCK   G + D RL+ +D+   R+L +PG     NVS  I CDKGERTRF
Sbjct: 20  FDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINCDKGERTRF 79

Query: 79  RSDVLSFQQMSEQFNQELSLTGKIPSGLFNSMFEF-SGSWQKDAANTKALAFDGVLITLY 137
           RSD+L F +MSE FNQ  S+TGKIPSG FN+ F F SGSW  DAAN K+L  D  ++TL+
Sbjct: 80  RSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGLDASVVTLF 139

Query: 138 TVALEK-SQMVLCDHVKKAVPASWDPPALSRFIETFGTHIIVGMKMGGKDVIYLKQQHSS 196
            + +   +++ L D V+ AVP+SWDP  L+RFIE +GTH+I G+ +GG+DV+ ++Q  SS
Sbjct: 140 NLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVVVVRQDKSS 199

Query: 197 ALQPADVQKRLKEMADRRFMDA--------NGQYSVASDQ-VFPNDKFGV--REQRLTFA 245
            L    ++  L ++ D+ F  +        N  Y  +  Q  FP + F V   +Q + F 
Sbjct: 200 DLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFP-EAFNVFDDKQTVAFN 258

Query: 246 NISPSSSYSHKEDIVSIYKRRGGSDDRNLSHHEWLQTVQVEPDVISMSFIPITSLLNGVP 305
           N S +S    +  I  I  +RGG D R  SH EWL TV  +PD I+ +FIPITSLL  VP
Sbjct: 259 NFSINS----QNGITVICAKRGG-DGRAKSHSEWLITVPDKPDAINFNFIPITSLLKDVP 313

Query: 306 GSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQWKQRSS-ASLQFSF 364
           GSG LSHA++LYLRYKPP+ +L  FL+F  PR WAPV ++LP G      S+  +L  +F
Sbjct: 314 GSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPALHINF 373

Query: 365 MGPKLYVNTTLVDVGKRPVTGLRLYLEGKKSNRLAIHLQHL-SSLPKIFQLVDDPNENFR 423
           MGPKLYVNTT V   K PVTG+R +LEGKK NRLAIHLQHL ++   + + + D  E+  
Sbjct: 374 MGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITD--EHIW 431

Query: 424 RKS----YDRRFYEKVQWKNFSHVCTAPVESD-------------EDLS-IVTGAQLQVE 465
           R S     + R++E +  K FSHVCT PV+ D              D++ IVTGAQL+V+
Sbjct: 432 RGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGAQLEVK 491

Query: 466 NYGIKNILFLRLRFSSVLGAKEVKHPEWDGSPGLGAKSGLISTLISQHFTATFQKP--PP 523
            +G K++L LRLR++ V     V++    G  G   KSG+ S++     + +        
Sbjct: 492 KHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVHHNMIQK 551

Query: 524 RAADVNINSAVYPGGPPVPVQAPKLLKFVDTTEMSRGPQENPGYWVVSGARLVVEKGRVS 583
              +V ++S V+PGGPPVP    K++KFVD +++ RGPQ +PG+W+V+G RL ++KG++ 
Sbjct: 552 DKNEVVLDSGVFPGGPPVPANN-KIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLDKGKLC 610

Query: 584 LRVKYSLL 591
           L VK++LL
Sbjct: 611 LHVKFALL 618


>AT4G24290.1 | Symbols:  | MAC/Perforin domain-containing protein |
           chr4:12594856-12596590 FORWARD LENGTH=350
          Length = 350

 Score =  456 bits (1173), Expect = e-128,   Method: Compositional matrix adjust.
 Identities = 225/336 (66%), Positives = 271/336 (80%), Gaps = 3/336 (0%)

Query: 1   MALKDPAHKXXXXXXXXXXXXYDISADLRLKYCKGDSIDSRLIEIDEHDVR-DLVLPGGV 59
           MAL+ PA K            YD++ DLRLKYCKG S DSRL++I E D   ++VLPGG+
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 60  SISNVSKSIKCDKGERTRFRSDVLSFQQMSEQFNQELSLTGKIPSGLFNSMFEFSGSWQK 119
           SI NVSKSIKCDKGER RFRSD+L FQQM+EQFNQELSL GKIPSGLFN+MFEFS  WQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 120 DAANTKALAFDGVLITLYTVALEKSQMVLCDHVKKAVPASWDPPALSRFIETFGTHIIVG 179
           DAA TK LAFDGV I+LY+VAL+KSQ++L +HVK+AVP++WDP AL+RFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 180 MKMGGKDVIYLKQQHSSALQPADVQKRLKEMADRRFMDANGQYSVASDQVFPNDKFGVRE 239
           +KMGGKDVIY KQQHSS LQP D+QKRLKE+AD+RF++A+  ++  S++V  + K   +E
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE 240

Query: 240 QRLTFANISPSSSYSHKEDIVSIYKRRGGSDDRNLSHHEWLQTVQVEPDVISMSFIPITS 299
           QRL FA+ S   SY++KED V + KRRGG+D+RNL H+EWLQTVQ+EPDVISMSFIPITS
Sbjct: 241 QRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 300

Query: 300 LLNGVPGSGFLSHAINLYLRYKP--PIEELHQFLEF 333
           LLNGVPGSGFLSHAINLYLR KP   +  +H FLE 
Sbjct: 301 LLNGVPGSGFLSHAINLYLRCKPINHLRFVHFFLEL 336


>AT1G29690.1 | Symbols: CAD1 | MAC/Perforin domain-containing
           protein | chr1:10379310-10381861 REVERSE LENGTH=561
          Length = 561

 Score =  449 bits (1156), Expect = e-126,   Method: Compositional matrix adjust.
 Identities = 258/591 (43%), Positives = 348/591 (58%), Gaps = 82/591 (13%)

Query: 22  YDISADLRLKYCKGDSIDSRLIEIDEHDVRDLVLPGGVSISNVSKSIKCDKGERTRFRSD 81
           +D+++D+RL YCKG +  SRL+ I+E   RDL L  G  + NV   I C +G     R  
Sbjct: 32  FDVTSDVRLLYCKG-APGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRGNSGTQRIS 90

Query: 82  VLSFQQMSEQFNQELSLTGKIPSGLFNSMFEFSGSWQKDAANTKALAFDGVLITLYTVAL 141
           V SF +M+E+FN    + G IP G FN+MF ++GSWQ DAA+TK+LA  G  I LY V L
Sbjct: 91  VCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYFIPLYDVKL 150

Query: 142 EKSQMVLCDHVKKAVPASWDPPALSRFIETFGTHIIVGMKMGGKDVIYLKQQHSSALQPA 201
            K  +VL + +++AVP+SWDP +L+ FIE +GTHI+  + +GG+DV+Y++Q  SS L  +
Sbjct: 151 AKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQHQSSPLPVS 210

Query: 202 DVQKRLKEMADRRFMDANGQYSVASDQVFPNDKFGVREQRLTFANISPSSSYSHKEDIVS 261
           +++  + +M   RF +A  Q                        +I+    Y  K DI  
Sbjct: 211 EIENYVNDMIKHRFHEAESQ------------------------SITGPLKYKDK-DITV 245

Query: 262 IYKRRGGSDDRNLSHHEWLQTVQVEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYK 321
           I++RRGG DD   SH  W +TV   PD+I+M+F PI SLL GVPG   L+ AI LYL YK
Sbjct: 246 IFRRRGG-DDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYK 304

Query: 322 PPIEELHQFLEFQLPRQWAPVFSELPLGPQWKQRSSASLQFSFMGPKLYVNTTLVDVGKR 381
           PPIE+L  FL++Q+ R WAP  S L    Q K+   +SLQFS MGPKL+++   V VG++
Sbjct: 305 PPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFISADQVTVGRK 360

Query: 382 PVTGLRLYLEGKKSNRLAIHLQHLSSLPKIFQLVDDPNENFRRKSY------DRRFYEKV 435
           PVTGLRL LEG K NRL+IHLQHL SLPKI Q   D +       +      D R++E +
Sbjct: 361 PVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDSRWFEPI 420

Query: 436 QWKNFSHVCTAPVESDE----DLS---IVTGAQLQVENYGIKNILFLRLRFSSVLGAKEV 488
           +WKNFSHV T+P+E  E    DLS   IVTGAQL V ++G KN+L L+L FS V G   +
Sbjct: 421 KWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCT-I 479

Query: 489 KHPEWDGSPGLGAKSGLISTLISQHFTATFQKPPPRAADVNINSAVYPGGPPVPV----- 543
           +   WD +P   A SG +                             PGGP         
Sbjct: 480 RRSVWDHTPV--ASSGRLE----------------------------PGGPSTSSSTEEV 509

Query: 544 --QAPKLLKFVDTTEMSRGPQENPGYWVVSGARLVVEKGRVSLRVKYSLLT 592
             Q+ KL K VD++EM +GPQ+ PG+W+V+GA+L VEKG++ LRVKYSLL 
Sbjct: 510 SGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLN 560