Miyakogusa Predicted Gene

Lj6g3v1966860.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj6g3v1966860.1 Non Chatacterized Hit- tr|I1MEX4|I1MEX4_SOYBN
Uncharacterized protein OS=Glycine max PE=4 SV=1,89.2,0,FAMILY NOT
NAMED,NULL; membrane-attack complex / perforin,Membrane attack complex
component/perforin,CUFF.60349.1
         (567 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G29690.1 | Symbols: CAD1 | MAC/Perforin domain-containing pro...   873   0.0  
AT4G24290.2 | Symbols:  | MAC/Perforin domain-containing protein...   440   e-123
AT1G28380.1 | Symbols: NSL1 | MAC/Perforin domain-containing pro...   408   e-114
AT1G14780.1 | Symbols:  | MAC/Perforin domain-containing protein...   390   e-108
AT4G24290.1 | Symbols:  | MAC/Perforin domain-containing protein...   231   1e-60

>AT1G29690.1 | Symbols: CAD1 | MAC/Perforin domain-containing
           protein | chr1:10379310-10381861 REVERSE LENGTH=561
          Length = 561

 Score =  873 bits (2256), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 420/560 (75%), Positives = 473/560 (84%), Gaps = 8/560 (1%)

Query: 8   STRSSDSLSATLCNSIQALGRGFDVTSDIRLLYCKGAPGSRLVRLDEEHTRDLAISHGVV 67
           S  SS++L+ TL N+IQALGRGFDVTSD+RLLYCKGAPGSRLVR++E   RDL +SHG +
Sbjct: 10  SVPSSEALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFL 69

Query: 68  VPNVSGDIVCSLGKSALEKTPVCSFHEMAKHFNERSGITGQIPLGSFNSMFNFTGSWVVD 127
           +PNV  DI CS G S  ++  VCSFHEMA+ FN RSG+ G IPLG FN+MFN+TGSW VD
Sbjct: 70  LPNVPADIDCSRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVD 129

Query: 128 AAATKSLAMVGTFIPLVEVKLAKLNLVLNDEVKRAVPYSWDPASLASFIENYGTHIVTSA 187
           AA+TKSLA+VG FIPL +VKLAKL LVL++E++RAVP SWDPASLASFIENYGTHIVTS 
Sbjct: 130 AASTKSLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSV 189

Query: 188 TVGGRDVVYIRQHQSSSLSASNIENYVKEIGDDRFFDGKNKSGPGPVKYKEKDVTVIFRR 247
           T+GGRDVVYIRQHQSS L  S IENYV ++   RF + +++S  GP+KYK+KD+TVIFRR
Sbjct: 190 TIGGRDVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDKDITVIFRR 249

Query: 248 RGGDDLEQSHTKWVETVKLAPDIINMKFTPIASLLEGVPGVKHLARAIELYLEYKPPIED 307
           RGGDDLEQSH +W ETV  APDIINM FTPI SLLEGVPG++HL RAIELYLEYKPPIED
Sbjct: 250 RGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIED 309

Query: 308 LQYFLDFQITRVWAPEQNNLQRKEPVCPSLQFSLMGPKLFVSPDQVTVGRKPVTGLRLSL 367
           LQYFLD+QI R WAPEQ+NLQRKEPVC SLQFSLMGPKLF+S DQVTVGRKPVTGLRLSL
Sbjct: 310 LQYFLDYQIARAWAPEQSNLQRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSL 369

Query: 368 EGSKQNRLAIHLQHLVSLPKIFQPHWETHMAIGAPKWQGPEEQDSRWFEPIKWKNFSHVS 427
           EGSKQNRL+IHLQHLVSLPKI QPHW++H+ IGAPKWQGPEEQDSRWFEPIKWKNFSHVS
Sbjct: 370 EGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDSRWFEPIKWKNFSHVS 429

Query: 428 TAPIEITETSIGDLSGVNIVTGAQLGVWDFGAKNVLHLKLLFSKVPGCTIRRSVWDHNPS 487
           T+PIE TET IGDLSGV+IVTGAQLGVWDFG+KNVLHLKLLFSKVPGCTIRRSVWDH P 
Sbjct: 430 TSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCTIRRSVWDHTPV 489

Query: 488 TPATAAQRSDGAXXXXXXXXXXXXXXXXXIHIGKLAKIVDMTEMFRGPQDIPGHWLVTGA 547
             A++ +   G                     GKLAKIVD +EM +GPQD+PGHWLVTGA
Sbjct: 490 --ASSGRLEPGGPSTSSSTEEVSGQS------GKLAKIVDSSEMLKGPQDLPGHWLVTGA 541

Query: 548 KLGVEKGKIVLRIKYSLLNY 567
           KLGVEKGKIVLR+KYSLLNY
Sbjct: 542 KLGVEKGKIVLRVKYSLLNY 561


>AT4G24290.2 | Symbols:  | MAC/Perforin domain-containing protein |
           chr4:12594856-12597815 FORWARD LENGTH=606
          Length = 606

 Score =  440 bits (1132), Expect = e-123,   Method: Compositional matrix adjust.
 Identities = 250/591 (42%), Positives = 341/591 (57%), Gaps = 60/591 (10%)

Query: 22  SIQALGRGFDVTSDIRLLYCKG-APGSRLVRLDE-EHTRDLAISHGVVVPNVSGDIVCSL 79
           +I ++G G+D+  D+RL YCKG +  SRL+ + E +   ++ +  G+ +PNVS  I C  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 80  GKSALEKTPVCSFHEMAKHFNERSGITGQIPLGSFNSMFNFTGSWVVDAAATKSLAMVGT 139
           G+    ++ +  F +MA+ FN+   + G+IP G FN+MF F+  W  DAA TK+LA  G 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 140 FIPLVEVKLAKLNLVLNDEVKRAVPYSWDPASLASFIENYGTHIVTSATVGGRDVVYIRQ 199
           FI L  V L K  ++L + VK+AVP +WDPA+LA FI+ YGTHI+ S  +GG+DV+Y +Q
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 200 HQSSSLSASNIENYVKEIGDDRFFDGK--------------------------NKSGPGP 233
             SS L   +++  +KE+ D RF +                            + S  G 
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 234 VKYKEKDVTVIFRRRGGDDLEQSHTKWVETVKLAPDIINMKFTPIASLLEGVPGVKHLAR 293
              KE  V +  RR G D+    H +W++TV++ PD+I+M F PI SLL GVPG   L+ 
Sbjct: 254 YANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLSH 313

Query: 294 AIELYLEYKPPIEDLQYFLDFQITRVWAPEQNNL----QRKEPVCPSLQFSLMGPKLFVS 349
           AI LYL YKPPIE+L  FL+FQ+ R WAP  + L    QRK+  C SLQFS  GPKL+V+
Sbjct: 314 AINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFSFFGPKLYVN 373

Query: 350 PDQVTVGRKPVTGLRLSLEGSKQNRLAIHLQHLVSLPKIFQPHWETHMAIGAPKWQGPEE 409
              V VG++P+TG+RL LEG + NRLAIHLQHL SLPKI+Q   + + +I        E 
Sbjct: 374 TTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSIRQ------ES 427

Query: 410 QDSRWFEPIKWKNFSHVSTAPIEITETSIGDLSGVNIVTGAQLGVWDFGAKNVLHLKLLF 469
            D R++E + WKN+SHV T P+E    S  DLS   +VTGAQL V   G KNVL L+L F
Sbjct: 428 HDRRYYEKVNWKNYSHVCTEPVE----SDDDLS---VVTGAQLHVESHGFKNVLFLRLCF 480

Query: 470 SKVPGCT-IRRSVWDHNP-------------STPATAAQR-SDGAXXXXXXXXXXXXXXX 514
           S+V G T ++ S WD                S   TAAQ+                    
Sbjct: 481 SRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPP 540

Query: 515 XXIHIGKLAKIVDMTEMFRGPQDIPGHWLVTGAKLGVEKGKIVLRIKYSLL 565
                 KL K VD +EM RGPQ+ PG+W+V+GA+L VEKGKI L++KYSL 
Sbjct: 541 VPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSLF 591


>AT1G28380.1 | Symbols: NSL1 | MAC/Perforin domain-containing
           protein | chr1:9963696-9966060 FORWARD LENGTH=612
          Length = 612

 Score =  408 bits (1048), Expect = e-114,   Method: Compositional matrix adjust.
 Identities = 237/596 (39%), Positives = 329/596 (55%), Gaps = 60/596 (10%)

Query: 22  SIQALGRGFDVTSDIRLLYCKGAP-GSRLVRLDEEHTRDLAISHGVVVPNVSGDIVCSLG 80
           ++  +G G+D+ SD+R   CK  P GSRLV +D    RDL    G+VV NVS  I C  G
Sbjct: 18  AVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKCDKG 77

Query: 81  KSALEKTPVCSFHEMAKHFNERSGITGQIPLGSFNSMFNFTGSWVVDAAATKSLAMVGTF 140
           +    ++ + SF++M++ FN+   ++G+IP G FN+MF F+  W  DA++ K+LA  G F
Sbjct: 78  ERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYDGWF 137

Query: 141 IPLVEVKLAKLNLVLNDEVKRAVPYSWDPASLASFIENYGTHIVTSATVGGRDVVYIRQH 200
           I L  V++ +  L L DEVKR VP SWD A+LA FIE YGTH+V   T+GG+DV++++Q 
Sbjct: 138 ISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHVKQM 197

Query: 201 QSSSLSASNIENYVKEIGDDRF-------------FDGKNKS------GPGP-------- 233
           + S+     I+  +K  GD+RF             + GK K       G  P        
Sbjct: 198 RKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPFGTSVSSA 257

Query: 234 --VKYKEKDVTVIFRRRGGDDLEQSHTKWVETVKLAPDIINMKFTPIASLLEGVPGVKHL 291
             +  K +++  +  RRGG DL QSH +W+ TV  AP++I+M F PI SLL G+PG   L
Sbjct: 258 VVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPGTGFL 317

Query: 292 ARAIELYLEYKPPIEDLQYFLDFQITRVWAPEQNN----LQRKEPVCPSLQFSLMGPKLF 347
           + A+ LYL YKPPIE+L  FL+FQ+ R WAP   +    L+R +   PSLQFSLMGPKL+
Sbjct: 318 SHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMGPKLY 377

Query: 348 VSPDQVTVGRKPVTGLRLSLEGSKQNRLAIHLQHLVSL-PKIFQPHWETHMAIGAPKWQG 406
           V+  +V  G +PVTGLR  LEG K N LAIHLQHL +  P +   H +T+  I  P  +G
Sbjct: 378 VNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEEPVEKG 437

Query: 407 PEEQDSRWFEPIKWKNFSHVSTAPIEITETSIGDLSGVNIVTGAQLGVWDFGAKNVLHLK 466
                  ++ P+KW  FSHV T P++       D +  +IVT A L V   G + VL L+
Sbjct: 438 -------YYVPVKWGIFSHVCTYPVQYNGARSDDTA--SIVTKAWLEVKGMGMRKVLFLR 488

Query: 467 LLFSKVPGCTIRRSVWDHNPSTPATAAQRSDGAXXXXXXXXXXXXXXXXXIHIG------ 520
           L FS       R+S WD+  +    +   S  +                   I       
Sbjct: 489 LGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKIDINSAVY 548

Query: 521 ----------KLAKIVDMTEMFRGPQDIPGHWLVTGAKLGVEKGKIVLRIKYSLLN 566
                     KL  +VD  E+ RGP++ PG+W+VTGAKL VE GKI ++ KYSLL 
Sbjct: 549 PRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLLT 604


>AT1G14780.1 | Symbols:  | MAC/Perforin domain-containing protein |
           chr1:5091020-5093873 FORWARD LENGTH=627
          Length = 627

 Score =  390 bits (1003), Expect = e-108,   Method: Compositional matrix adjust.
 Identities = 236/618 (38%), Positives = 332/618 (53%), Gaps = 83/618 (13%)

Query: 22  SIQALGRGFDVTSDIRLLYCKGAPGS----RLVRLDEEHTRDLAISHGVVVPNVSGDIVC 77
           ++++LG+GFD+T+D RL YCK   GS    RLV LD+   R+L I    V  NVS DI C
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 78  SLGKSALEKTPVCSFHEMAKHFNERSGITGQIPLGSFNSMFNF-TGSWVVDAAATKSLAM 136
             G+    ++ +  F++M+++FN+RS +TG+IP G+FN+ F F +GSW  DAA  KSL +
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 137 VGTFIPLVEVKLAKLN-LVLNDEVKRAVPYSWDPASLASFIENYGTHIVTSATVGGRDVV 195
             + + L  + +   N L L D V+ AVP SWDP  LA FIE YGTH++T  +VGG+DVV
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 196 YIRQHQSSSLSASNIENYVKEIGDDRF------------------------------FDG 225
            +RQ +SS L    + +++ ++GD  F                              FD 
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVFDD 251

Query: 226 KNKSGPGPVKYKEKD-VTVIFRRRGGDDLEQSHTKWVETVKLAPDIINMKFTPIASLLEG 284
           K            ++ +TVI  +RGGD   +SH++W+ TV   PD IN  F PI SLL+ 
Sbjct: 252 KQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSLLKD 311

Query: 285 VPGVKHLARAIELYLEYKPPIEDLQYFLDFQITRVWAPEQNNL-----QRKEPVCPSLQF 339
           VPG   L+ A+ LYL YKPP+ DLQYFLDF   R WAP  N+L            P+L  
Sbjct: 312 VPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPALHI 371

Query: 340 SLMGPKLFVSPDQVTVGRKPVTGLRLSLEGSKQNRLAIHLQHLVSL-PKIFQPHWETHMA 398
           + MGPKL+V+   VT  + PVTG+R  LEG K NRLAIHLQHL +    + +   + H+ 
Sbjct: 372 NFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEHI- 430

Query: 399 IGAPKWQGPEE--QDSRWFEPIKWKNFSHVSTAPIEITETSIGDLSG-------VNIVTG 449
                W+G ++   + R+FEP+  K FSHV T P++     I   S          IVTG
Sbjct: 431 -----WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 485

Query: 450 AQLGVWDFGAKNVLHLKLLFSKVPGCTIRRSVWDHNP-------------STPATAAQRS 496
           AQL V   G+K+VLHL+L ++KV    + ++ W H P             S P T    S
Sbjct: 486 AQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLT----S 541

Query: 497 DGAXXXXXXXXXXXXXXXXXIHIG--------KLAKIVDMTEMFRGPQDIPGHWLVTGAK 548
                               +  G        K+ K VD++++ RGPQ  PGHWLVTG +
Sbjct: 542 GSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLVTGVR 601

Query: 549 LGVEKGKIVLRIKYSLLN 566
           L ++KGK+ L +K++LL+
Sbjct: 602 LYLDKGKLCLHVKFALLH 619


>AT4G24290.1 | Symbols:  | MAC/Perforin domain-containing protein |
           chr4:12594856-12596590 FORWARD LENGTH=350
          Length = 350

 Score =  231 bits (589), Expect = 1e-60,   Method: Compositional matrix adjust.
 Identities = 123/323 (38%), Positives = 183/323 (56%), Gaps = 30/323 (9%)

Query: 22  SIQALGRGFDVTSDIRLLYCKG-APGSRLVRLDE-EHTRDLAISHGVVVPNVSGDIVCSL 79
           +I ++G G+D+  D+RL YCKG +  SRL+ + E +   ++ +  G+ +PNVS  I C  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 80  GKSALEKTPVCSFHEMAKHFNERSGITGQIPLGSFNSMFNFTGSWVVDAAATKSLAMVGT 139
           G+    ++ +  F +MA+ FN+   + G+IP G FN+MF F+  W  DAA TK+LA  G 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 140 FIPLVEVKLAKLNLVLNDEVKRAVPYSWDPASLASFIENYGTHIVTSATVGGRDVVYIRQ 199
           FI L  V L K  ++L + VK+AVP +WDPA+LA FI+ YGTHI+ S  +GG+DV+Y +Q
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 200 HQSSSLSASNIENYVKEIGDDRFFDGK--------------------------NKSGPGP 233
             SS L   +++  +KE+ D RF +                            + S  G 
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 234 VKYKEKDVTVIFRRRGGDDLEQSHTKWVETVKLAPDIINMKFTPIASLLEGVPGVKHLAR 293
              KE  V +  RR G D+    H +W++TV++ PD+I+M F PI SLL GVPG   L+ 
Sbjct: 254 YANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLSH 313

Query: 294 AIELYLEYKP--PIEDLQYFLDF 314
           AI LYL  KP   +  + +FL+ 
Sbjct: 314 AINLYLRCKPINHLRFVHFFLEL 336