Miyakogusa Predicted Gene

Lj6g3v1693500.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj6g3v1693500.1 Non Chatacterized Hit- tr|I1L088|I1L088_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.43844
PE,92.58,0,Vps53_N,Vps53-like, N-terminal; seg,NULL; SUBFAMILY NOT
NAMED,NULL; VACUOLAR SORTING PROTEIN 53,NULL,CUFF.59849.1
         (565 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G50500.2 | Symbols: HIT1 | Membrane trafficking VPS53 family ...   841   0.0  
AT1G50500.1 | Symbols: HIT1, ATVPS53, VPS53 | Membrane trafficki...   841   0.0  
AT1G50970.1 | Symbols:  | Membrane trafficking VPS53 family prot...   140   2e-33

>AT1G50500.2 | Symbols: HIT1 | Membrane trafficking VPS53 family
           protein | chr1:18708217-18715597 REVERSE LENGTH=847
          Length = 847

 Score =  841 bits (2173), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 410/573 (71%), Positives = 460/573 (80%), Gaps = 9/573 (1%)

Query: 1   MRSNEEIWKIFPSSWHVSYRLCILFCKKTRKQLEDILANLKEKPDVGTLLLALQRTLEFE 60
           +R+NEEIWKIFP+SWHV YRLCI FCK+TRKQ+E IL N+KEKP V  LLLALQ T+EFE
Sbjct: 274 IRTNEEIWKIFPASWHVPYRLCIQFCKQTRKQVESILVNMKEKPVVAILLLALQSTVEFE 333

Query: 61  DELAEKFGGGTQNREVANAIEEIGRXXXXXXXXXDIRKKYEKKLAAQQGSE----SEEKD 116
            EL +KFGGG   +++ + IEEIG           IRKKYEKK AA Q +E     +EK 
Sbjct: 334 KELEKKFGGGVPTKDIEDDIEEIGTWEDNSQNISKIRKKYEKKFAASQETEENGFQQEKT 393

Query: 117 GSKDLAVPGAGFNFRGIISSCFEPHLQVYIELEEKTLMESLEKLVQEETWDIEEGGQSSV 176
           G+KDL+V GAGFNFRG+ISSCFEPHL  YIELEEKTLM+ LEK+VQEE+WD+E+G Q++V
Sbjct: 394 GNKDLSVTGAGFNFRGMISSCFEPHLTPYIELEEKTLMDDLEKIVQEESWDVEDGSQNNV 453

Query: 177 LSSSMQLFLIIKRSLKRCSALTKNQTLYNLLKVFQRILKAYATKLFARLPKGGTGIVAAA 236
           LSSS QLF  IK+SLKRC+ L+KNQTL+NL KVFQR+LKAYATKLF +LPKGGTGIVAAA
Sbjct: 454 LSSSTQLFSNIKKSLKRCNTLSKNQTLFNLFKVFQRVLKAYATKLFFKLPKGGTGIVAAA 513

Query: 237 TGMDGQIKTSDRDERVICYIVNSAEYCHKTAGELAESVSKIIDPQFADGVDMSEVQDEFS 296
           TGMDGQIK S+RDERVICYIVNSAEYCHKT+GELAE+VS+IIDP +ADGVDMSEVQDEFS
Sbjct: 514 TGMDGQIKVSERDERVICYIVNSAEYCHKTSGELAENVSEIIDPHYADGVDMSEVQDEFS 573

Query: 297 AVITRSLVTLVHGLETKFDIEMAAMTRVPWGTLESVGDQSEYVNSINLILTTSIPTLGSL 356
           AVIT++LVTLV GLETKFD EMA MTRVPW TLESVGDQS YVN IN +L+ SIP LG L
Sbjct: 574 AVITKALVTLVLGLETKFDTEMAVMTRVPWSTLESVGDQSGYVNGINTVLSGSIPVLGKL 633

Query: 357 LSPLYFQFFLDKLASSLGPRFYSNIFKCKQISETGAQQMLLDTQAVKTILLEIPSLGRQT 416
           L+P+YFQFFLDKLASSLGPRFY+NIF+CKQ+SETGAQQMLLDTQAVK+ILLEIPSL RQT
Sbjct: 634 LTPVYFQFFLDKLASSLGPRFYANIFRCKQLSETGAQQMLLDTQAVKSILLEIPSLARQT 693

Query: 417 SGAASYSKFVSREMSKAEALLKVILSPVDSVADTYRALLPEGTPMEFQRILELKGLKKAD 476
           S AASYSKFVSREMS+AEALLKVILSP+DSVADTYRAL PEGTPMEFQRILELKGLKKAD
Sbjct: 694 STAASYSKFVSREMSRAEALLKVILSPIDSVADTYRALFPEGTPMEFQRILELKGLKKAD 753

Query: 477 QQSILDDFNKHGPGIKQTQXXXXXXXXXXXXXXXXXXXXXXXXXX-----REDVXXXXXX 531
           QQSILDDFNKHGPG  Q                                  EDV      
Sbjct: 754 QQSILDDFNKHGPGFTQQSVAAAMPQPMPTPPAPPLAITNPATAAGFIANSEDVLTRAAA 813

Query: 532 XXXXXXXXXFKRFLALTEAAKDRKDGPFRKLFN 564
                    FK+F+ALTEAAKDRKDGP R+LFN
Sbjct: 814 LGRGAASTGFKKFIALTEAAKDRKDGPLRRLFN 846


>AT1G50500.1 | Symbols: HIT1, ATVPS53, VPS53 | Membrane trafficking
           VPS53 family protein | chr1:18708217-18715597 REVERSE
           LENGTH=828
          Length = 828

 Score =  841 bits (2172), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 410/573 (71%), Positives = 460/573 (80%), Gaps = 9/573 (1%)

Query: 1   MRSNEEIWKIFPSSWHVSYRLCILFCKKTRKQLEDILANLKEKPDVGTLLLALQRTLEFE 60
           +R+NEEIWKIFP+SWHV YRLCI FCK+TRKQ+E IL N+KEKP V  LLLALQ T+EFE
Sbjct: 255 IRTNEEIWKIFPASWHVPYRLCIQFCKQTRKQVESILVNMKEKPVVAILLLALQSTVEFE 314

Query: 61  DELAEKFGGGTQNREVANAIEEIGRXXXXXXXXXDIRKKYEKKLAAQQGSE----SEEKD 116
            EL +KFGGG   +++ + IEEIG           IRKKYEKK AA Q +E     +EK 
Sbjct: 315 KELEKKFGGGVPTKDIEDDIEEIGTWEDNSQNISKIRKKYEKKFAASQETEENGFQQEKT 374

Query: 117 GSKDLAVPGAGFNFRGIISSCFEPHLQVYIELEEKTLMESLEKLVQEETWDIEEGGQSSV 176
           G+KDL+V GAGFNFRG+ISSCFEPHL  YIELEEKTLM+ LEK+VQEE+WD+E+G Q++V
Sbjct: 375 GNKDLSVTGAGFNFRGMISSCFEPHLTPYIELEEKTLMDDLEKIVQEESWDVEDGSQNNV 434

Query: 177 LSSSMQLFLIIKRSLKRCSALTKNQTLYNLLKVFQRILKAYATKLFARLPKGGTGIVAAA 236
           LSSS QLF  IK+SLKRC+ L+KNQTL+NL KVFQR+LKAYATKLF +LPKGGTGIVAAA
Sbjct: 435 LSSSTQLFSNIKKSLKRCNTLSKNQTLFNLFKVFQRVLKAYATKLFFKLPKGGTGIVAAA 494

Query: 237 TGMDGQIKTSDRDERVICYIVNSAEYCHKTAGELAESVSKIIDPQFADGVDMSEVQDEFS 296
           TGMDGQIK S+RDERVICYIVNSAEYCHKT+GELAE+VS+IIDP +ADGVDMSEVQDEFS
Sbjct: 495 TGMDGQIKVSERDERVICYIVNSAEYCHKTSGELAENVSEIIDPHYADGVDMSEVQDEFS 554

Query: 297 AVITRSLVTLVHGLETKFDIEMAAMTRVPWGTLESVGDQSEYVNSINLILTTSIPTLGSL 356
           AVIT++LVTLV GLETKFD EMA MTRVPW TLESVGDQS YVN IN +L+ SIP LG L
Sbjct: 555 AVITKALVTLVLGLETKFDTEMAVMTRVPWSTLESVGDQSGYVNGINTVLSGSIPVLGKL 614

Query: 357 LSPLYFQFFLDKLASSLGPRFYSNIFKCKQISETGAQQMLLDTQAVKTILLEIPSLGRQT 416
           L+P+YFQFFLDKLASSLGPRFY+NIF+CKQ+SETGAQQMLLDTQAVK+ILLEIPSL RQT
Sbjct: 615 LTPVYFQFFLDKLASSLGPRFYANIFRCKQLSETGAQQMLLDTQAVKSILLEIPSLARQT 674

Query: 417 SGAASYSKFVSREMSKAEALLKVILSPVDSVADTYRALLPEGTPMEFQRILELKGLKKAD 476
           S AASYSKFVSREMS+AEALLKVILSP+DSVADTYRAL PEGTPMEFQRILELKGLKKAD
Sbjct: 675 STAASYSKFVSREMSRAEALLKVILSPIDSVADTYRALFPEGTPMEFQRILELKGLKKAD 734

Query: 477 QQSILDDFNKHGPGIKQTQXXXXXXXXXXXXXXXXXXXXXXXXXX-----REDVXXXXXX 531
           QQSILDDFNKHGPG  Q                                  EDV      
Sbjct: 735 QQSILDDFNKHGPGFTQQSVAAAMPQPMPTPPAPPLAITNPATAAGFIANSEDVLTRAAA 794

Query: 532 XXXXXXXXXFKRFLALTEAAKDRKDGPFRKLFN 564
                    FK+F+ALTEAAKDRKDGP R+LFN
Sbjct: 795 LGRGAASTGFKKFIALTEAAKDRKDGPLRRLFN 827


>AT1G50970.1 | Symbols:  | Membrane trafficking VPS53 family protein
           | chr1:18896323-18900268 FORWARD LENGTH=569
          Length = 569

 Score =  140 bits (354), Expect = 2e-33,   Method: Compositional matrix adjust.
 Identities = 83/186 (44%), Positives = 96/186 (51%), Gaps = 14/186 (7%)

Query: 388 SETGAQQMLLDTQAVKTILLEIPSLGRQ--------TSGAASYSKFVSREMSKAEALLKV 439
           SE     MLLD   +K ILL++PSL RQ         +  ASY K V+ +M +AEA+LKV
Sbjct: 390 SENSVYLMLLDAHDMKMILLKVPSLARQPEASALLVKTATASYVKLVNHQMKRAEAVLKV 449

Query: 440 ILSPVDSVADTYRALLPEGTPMEFQRILELKGLKKADQQSILDDFNKHGPGIKQTQXXXX 499
           I SP+ +V DTYRAL PE TPMEFQRIL LKGL KA+QQSILDDFN H   I Q      
Sbjct: 450 IASPIVTVIDTYRALFPEETPMEFQRILVLKGLTKAEQQSILDDFNNHSSRITQLSVAAK 509

Query: 500 XXXXXXXXXXXXXXXXXXXXXXREDVXXXXXXXXXXXXXXXFKRFLALTEAAKDRKDGPF 559
                                   +                F +  ALT AAKDR   PF
Sbjct: 510 TPEAHALPLALTNVAPAVRFKANSE---EVLTRAASAATTSFMKLYALTGAAKDR---PF 563

Query: 560 RKLFNP 565
           RKLFNP
Sbjct: 564 RKLFNP 569



 Score =  138 bits (348), Expect = 9e-33,   Method: Compositional matrix adjust.
 Identities = 77/170 (45%), Positives = 91/170 (53%), Gaps = 40/170 (23%)

Query: 1   MRSNEEIWKIFPSSWHVSYRLCILFCKKTRKQLEDILANLKEKPDVGTLLLALQRTLEFE 60
           +R N+  W IFP+SWHV YRLCI   +KTR Q+E IL NLKEK DV  LLL L+RTLEFE
Sbjct: 255 IRKNQGKWTIFPASWHVPYRLCIQLSRKTRVQVESILVNLKEKSDVEKLLLELKRTLEFE 314

Query: 61  DELAEKFGGGTQNREVANAIEEIGRXXXXXXXXXDIRKKYEKKLAAQQGSESEEKDGSKD 120
            EL                                   K+    +          + S+ 
Sbjct: 315 REL---------------------------------EMKFGGGGSIGDDIIGGGGNNSQ- 340

Query: 121 LAVPGAGFNFRGIISSCFEPHLQVYIELEEKTLMESLEKLVQEETWDIEE 170
                  FNFRG+ISSCFEPHL +YIE EE  LM+ LEK+VQEETWDIEE
Sbjct: 341 ------KFNFRGMISSCFEPHLTIYIEKEEMELMQLLEKVVQEETWDIEE 384