Miyakogusa Predicted Gene

Lj6g3v0574710.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj6g3v0574710.1 tr|E8RUL4|E8RUL4_ASTEC Glycoside hydrolase clan
GH-D (Precursor) OS=Asticcacaulis excentricus
(strai,36.97,2e-18,
ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE,NULL; no
description,Aldolase-type TIM barrel;
n,NODE_15428_length_2284_cov_74.968918.path2.1
         (451 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G26380.1 | Symbols:  | Melibiase family protein | chr3:966014...   478   e-135
AT5G08370.2 | Symbols: AtAGAL2, AGAL2 | alpha-galactosidase 2 | ...    59   8e-09
AT5G08370.1 | Symbols: AtAGAL2, AGAL2 | alpha-galactosidase 2 | ...    59   9e-09
AT3G56310.2 | Symbols:  | Melibiase family protein | chr3:208828...    55   1e-07
AT3G56310.1 | Symbols:  | Melibiase family protein | chr3:208828...    55   1e-07
AT5G08380.1 | Symbols: AtAGAL1, AGAL1 | alpha-galactosidase 1 | ...    49   7e-06

>AT3G26380.1 | Symbols:  | Melibiase family protein |
           chr3:9660140-9663145 FORWARD LENGTH=647
          Length = 647

 Score =  478 bits (1231), Expect = e-135,   Method: Compositional matrix adjust.
 Identities = 236/429 (55%), Positives = 302/429 (70%), Gaps = 15/429 (3%)

Query: 30  VKHDCVFGDDLDLNEISYVSEVLRELGRPIVYSLSPGTSVTPAMAKDISGLVNMYRITAD 89
           +KHDCVFG D ++ EI+YVSEVL+EL RP++YS+SPGTSVTP MAK++S LVNMYRIT D
Sbjct: 223 IKHDCVFGTDFNIEEITYVSEVLKELDRPVLYSISPGTSVTPTMAKEVSQLVNMYRITGD 282

Query: 90  DWDNWGDVKTHFDVTRDFSTANMIGAKGLMGNSWPDLDMLPFGWLTDPGSNEGPHRYTNL 149
           DWD W DV  HFD++RD S ++MIGA+GL G SWPDLDMLP GWLTD GSN GPHR  NL
Sbjct: 283 DWDTWKDVTAHFDISRDLSASSMIGARGLQGKSWPDLDMLPLGWLTDQGSNVGPHRACNL 342

Query: 150 NLQEQRTQMTLWSMAKTPLMYGGDVRKIDSTTYDLITNPTLLEINSFSSNNMELPYITIL 209
           NL+EQ++QMTLWS+AK+PLM+GGDVR +D+TTY+LITNPTLLEINS+SSNN E PYIT  
Sbjct: 343 NLEEQKSQMTLWSIAKSPLMFGGDVRNLDATTYNLITNPTLLEINSYSSNNKEFPYITAT 402

Query: 210 KSEDQDLGRQMRRSYRE----KETSYKYSLGLTSCSESKASGWTSESYNGYLERICWKRS 265
           +     + R   + Y      K  S K++ GLTSC E KA+ W     N    +ICW + 
Sbjct: 403 R-----VSRNKHKGYPHHPTGKNISTKHAFGLTSCKEQKANTWFIVDKN--RGQICWNQH 455

Query: 266 FKNKHLAPFCVQKRELNLTFKKESMYHEDRQRGHHLVAANRIKFCLDASPRRKLSSLEFR 325
              K   PFC+  R+  L   K+  +++  Q   HL   ++ + CL AS ++KL+S ++ 
Sbjct: 456 SSEKLEKPFCLYNRKALLASDKKLKHNQLYQGKLHLHTNDKAQSCLAASSQQKLTSKDYS 515

Query: 326 RCTFSPCRLDSNQMWELNPNGTLVNSYSGLCATMESVKAT-ITSGGFRSWIATGRAGKVY 384
           +   SPC+LD+NQMWEL+ NGTL NSYSGLCA +  VKA   +S G RSWIATGR G+VY
Sbjct: 516 QGALSPCKLDANQMWELHSNGTLENSYSGLCAVLNPVKAAEASSNGVRSWIATGRRGEVY 575

Query: 385 VAFFNLSEQKAVMSVQTSDLAKVLPGKD---FSSCKGSEIWSGSDTVIMQGTLSTAVEVH 441
           VAFFNL+++K  +S + SD+A  L GK     +SC   E+WSG D    + ++S  VE H
Sbjct: 576 VAFFNLNQEKTKISAKISDIATALRGKKNLVGASCTSHELWSGKDFGPTKDSVSIQVEPH 635

Query: 442 GSALFVLNC 450
           G ALFVL+C
Sbjct: 636 GPALFVLHC 644


>AT5G08370.2 | Symbols: AtAGAL2, AGAL2 | alpha-galactosidase 2 |
           chr5:2691116-2693441 REVERSE LENGTH=370
          Length = 370

 Score = 58.5 bits (140), Expect = 8e-09,   Method: Compositional matrix adjust.
 Identities = 44/148 (29%), Positives = 69/148 (46%), Gaps = 24/148 (16%)

Query: 48  VSEVLRELGRPIVYSLSPGTSVTPAM-AKDISGLVNMYRITADDWDNWGDVKTHFDVTRD 106
           +S+ L   GR I +SL       PA  A DI    N +R T D  DNW  +    D    
Sbjct: 151 MSKALLNSGRSIFFSLCEWGQEDPATWAGDIG---NSWRTTGDIQDNWKSMTLIADQNDR 207

Query: 107 FSTANMIGAKGLMGNSWPDLDMLPFGWLTDPGSNEGPHRYTNLNLQEQRTQMTLWSMAKT 166
           +++    G       SW D DML  G       N G      +  +E  +  ++W++AK 
Sbjct: 208 WASYARPG-------SWNDPDMLEVG-------NGG------MTKEEYMSHFSIWALAKA 247

Query: 167 PLMYGGDVRKIDSTTYDLITNPTLLEIN 194
           PL+ G D+R +D  T++L++N  ++ +N
Sbjct: 248 PLLIGCDLRSMDKVTFELLSNKEVIAVN 275


>AT5G08370.1 | Symbols: AtAGAL2, AGAL2 | alpha-galactosidase 2 |
           chr5:2691116-2693441 REVERSE LENGTH=396
          Length = 396

 Score = 58.5 bits (140), Expect = 9e-09,   Method: Compositional matrix adjust.
 Identities = 44/148 (29%), Positives = 69/148 (46%), Gaps = 24/148 (16%)

Query: 48  VSEVLRELGRPIVYSLSPGTSVTPAM-AKDISGLVNMYRITADDWDNWGDVKTHFDVTRD 106
           +S+ L   GR I +SL       PA  A DI    N +R T D  DNW  +    D    
Sbjct: 177 MSKALLNSGRSIFFSLCEWGQEDPATWAGDIG---NSWRTTGDIQDNWKSMTLIADQNDR 233

Query: 107 FSTANMIGAKGLMGNSWPDLDMLPFGWLTDPGSNEGPHRYTNLNLQEQRTQMTLWSMAKT 166
           +++    G       SW D DML  G       N G      +  +E  +  ++W++AK 
Sbjct: 234 WASYARPG-------SWNDPDMLEVG-------NGG------MTKEEYMSHFSIWALAKA 273

Query: 167 PLMYGGDVRKIDSTTYDLITNPTLLEIN 194
           PL+ G D+R +D  T++L++N  ++ +N
Sbjct: 274 PLLIGCDLRSMDKVTFELLSNKEVIAVN 301


>AT3G56310.2 | Symbols:  | Melibiase family protein |
           chr3:20882886-20885745 FORWARD LENGTH=413
          Length = 413

 Score = 54.7 bits (130), Expect = 1e-07,   Method: Compositional matrix adjust.
 Identities = 43/146 (29%), Positives = 67/146 (45%), Gaps = 24/146 (16%)

Query: 50  EVLRELGRPIVYSLSPGTSVTPAM-AKDISGLVNMYRITADDWDNWGDVKTHFDVTRDFS 108
           + L   GR I YSL       PA+ AK++    N +R T D  D W  + T  D+   + 
Sbjct: 187 DALNATGRSIFYSLCEWGVDDPALWAKEVG---NSWRTTDDINDTWASMTTIADLNNKW- 242

Query: 109 TANMIGAKGLMGNSWPDLDMLPFGWLTDPGSNEGPHRYTNLNLQEQRTQMTLWSMAKTPL 168
            A   G  G     W D DML  G       N G      +  +E R   ++W++ K PL
Sbjct: 243 -AAYAGPGG-----WNDPDMLEIG-------NGG------MTYEEYRGHFSIWALMKAPL 283

Query: 169 MYGGDVRKIDSTTYDLITNPTLLEIN 194
           + G DVR + + T ++++N  ++ +N
Sbjct: 284 LIGCDVRNMTAETLEILSNKEIIAVN 309


>AT3G56310.1 | Symbols:  | Melibiase family protein |
           chr3:20882886-20885745 FORWARD LENGTH=437
          Length = 437

 Score = 54.7 bits (130), Expect = 1e-07,   Method: Compositional matrix adjust.
 Identities = 43/146 (29%), Positives = 67/146 (45%), Gaps = 24/146 (16%)

Query: 50  EVLRELGRPIVYSLSPGTSVTPAM-AKDISGLVNMYRITADDWDNWGDVKTHFDVTRDFS 108
           + L   GR I YSL       PA+ AK++    N +R T D  D W  + T  D+   + 
Sbjct: 211 DALNATGRSIFYSLCEWGVDDPALWAKEVG---NSWRTTDDINDTWASMTTIADLNNKW- 266

Query: 109 TANMIGAKGLMGNSWPDLDMLPFGWLTDPGSNEGPHRYTNLNLQEQRTQMTLWSMAKTPL 168
            A   G  G     W D DML  G       N G      +  +E R   ++W++ K PL
Sbjct: 267 -AAYAGPGG-----WNDPDMLEIG-------NGG------MTYEEYRGHFSIWALMKAPL 307

Query: 169 MYGGDVRKIDSTTYDLITNPTLLEIN 194
           + G DVR + + T ++++N  ++ +N
Sbjct: 308 LIGCDVRNMTAETLEILSNKEIIAVN 333


>AT5G08380.1 | Symbols: AtAGAL1, AGAL1 | alpha-galactosidase 1 |
           chr5:2694851-2697616 REVERSE LENGTH=410
          Length = 410

 Score = 48.9 bits (115), Expect = 7e-06,   Method: Compositional matrix adjust.
 Identities = 39/147 (26%), Positives = 65/147 (44%), Gaps = 22/147 (14%)

Query: 48  VSEVLRELGRPIVYSLSPGTSVTPAMAKDISGLVNMYRITADDWDNWGDVKTHFDVTRDF 107
           ++  L + GRPI +SL     + PA+     G  N +R T D  D W  + +  D+    
Sbjct: 191 MTRALMKSGRPIFHSLCEWGDMHPALWGSPVG--NSWRTTNDIKDTWLSMISIADM---- 244

Query: 108 STANMIGAKGLMGNSWPDLDMLPFGWLTDPGSNEGPHRYTNLNLQEQRTQMTLWSMAKTP 167
              N + A+      W D DML  G       N G      +   E     ++W+++K P
Sbjct: 245 ---NEVYAEHARPGGWNDPDMLEVG-------NGG------MTKDEYIVHFSIWAISKAP 288

Query: 168 LMYGGDVRKIDSTTYDLITNPTLLEIN 194
           L+ G D+R +   T +++ N  ++ IN
Sbjct: 289 LLLGCDIRNMTKETMEIVANKEVIAIN 315