Miyakogusa Predicted Gene

Lj5g3v2298240.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v2298240.1 tr|I1NJA5|I1NJA5_SOYBN 2-isopropylmalate synthase
OS=Glycine max GN=leuA PE=3 SV=1,83.09,0,2-ISOPROPYLMALATE
SYNTHASE,NULL; HOMOCITRATE SYNTHASE-RELATED,NULL; no
description,Aldolase-type TIM,CUFF.57326.1
         (621 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G74040.1 | Symbols: IMS1, MAML-3, IPMS2 | 2-isopropylmalate s...   936   0.0  
AT1G18500.1 | Symbols: MAML-4, IPMS1 | methylthioalkylmalate syn...   911   0.0  
AT5G23010.1 | Symbols: MAM1, IMS3 | methylthioalkylmalate syntha...   545   e-155
AT5G23020.1 | Symbols: MAM-L, MAM3, IMS2 | 2-isopropylmalate syn...   539   e-153

>AT1G74040.1 | Symbols: IMS1, MAML-3, IPMS2 | 2-isopropylmalate
           synthase 1 | chr1:27842258-27845566 FORWARD LENGTH=631
          Length = 631

 Score =  936 bits (2419), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 448/591 (75%), Positives = 512/591 (86%), Gaps = 6/591 (1%)

Query: 37  SKQHRYCSSSSYPKFTVS-----CSQFQPETTRRRPPYIPNHIPDPSYVRIFDTTLRDGE 91
           S+ HR  S ++  KF VS      S   P   RRRP YIPN I DP+YVRIFDTTLRDGE
Sbjct: 39  SQNHRTISLTTAGKFRVSYSLSASSPLPPHAPRRRPNYIPNRISDPNYVRIFDTTLRDGE 98

Query: 92  QSPGASMTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKMIAQEVGNAVDDEGYVP 151
           QSPGA++TSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVK IA+ VGN VD+ GYVP
Sbjct: 99  QSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAETVGNTVDENGYVP 158

Query: 152 VICGLSRCNEKDIRTAWDAVKHAKRPRIHTFIATSGIHMEYKLRMSKEQVIDKARSMVKF 211
           VICGLSRCN+KDI TAW+AVK+AKRPRIHTFIATS IH++YKL+ SKE+VI+ AR+MV+F
Sbjct: 159 VICGLSRCNKKDIETAWEAVKYAKRPRIHTFIATSDIHLKYKLKKSKEEVIEIARNMVRF 218

Query: 212 ARSLGCNDVEFSPEDAGRSEREFLYEILAEVIKAGATTLNIPDTVGITMPSEFGKLIADI 271
           ARSLGC DVEFSPEDAGRSERE+LYEIL EVIKAGATTLNIPDTVGIT+PSEFG+LIADI
Sbjct: 219 ARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLIADI 278

Query: 272 KANTPGAENVVISTHCQNDLGLSTANTIEGAHAGARQLEVTINGIGERAGNASLEEVVMA 331
           KANTPG +NV+ISTHCQNDLGLSTANT+ GAH+GARQ+EVTINGIGERAGNASLEEVVMA
Sbjct: 279 KANTPGIQNVIISTHCQNDLGLSTANTLSGAHSGARQVEVTINGIGERAGNASLEEVVMA 338

Query: 332 LKC-GGHVFGNLYTGVNAKHIYLTSRMVEEYSGLQLQPHKALVGANAFAHESGIHQDGML 390
           +KC G HV G L+TG++ +HI +TS+MVEEY+G+Q QPHKA+VGANAFAHESGIHQDGML
Sbjct: 339 IKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIHQDGML 398

Query: 391 KHKGTYEIISPEDIGLERSNEAGIVLGKLSGRHALRKRLQELGYELKDDQVESLFWRFKA 450
           KHKGTYEI+SPE+IGLERSN+AGIVLGKLSGRHAL+ RL ELGY L D Q+ +LFWRFKA
Sbjct: 399 KHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVLDDGQLSNLFWRFKA 458

Query: 451 VAEQKKRVTDADLRALVSDEVFQAEPVWKLGDLQVTCGTLGLSTATVKLVSDDGSTHVAC 510
           VAEQKKRVTDADL ALVSDEVFQ E VWKL D+Q+TCGTLGLST+TVKL   DG  HVAC
Sbjct: 459 VAEQKKRVTDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTSTVKLADSDGKEHVAC 518

Query: 511 AIGTGPVDSAYKAVDLIVKEPITLLEYSMNAVTEGIDAIATTRVVIRGETDHTSTHALTG 570
           ++GTGPVD+AYKAVDLIVKEP TLLEYSMNAVTEGIDAIATTRV+IRG+ +++ST+A+TG
Sbjct: 519 SVGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSSTNAVTG 578

Query: 571 ETVHRTFGGSGAGMDXXXXXXXXXXXXINKMLGFKDTSPSTEKIPVSASKV 621
           E+V RTF G+GAGMD            +NKMLGFK+ + +  K P+  ++V
Sbjct: 579 ESVERTFSGTGAGMDIVVSSVKAYVGALNKMLGFKEHTSTLSKTPLETNEV 629


>AT1G18500.1 | Symbols: MAML-4, IPMS1 | methylthioalkylmalate
           synthase-like 4 | chr1:6369347-6372861 FORWARD
           LENGTH=631
          Length = 631

 Score =  911 bits (2354), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 434/554 (78%), Positives = 488/554 (88%), Gaps = 3/554 (0%)

Query: 69  YIPNHIPDPSYVRIFDTTLRDGEQSPGASMTSKEKLDIARQLAKLGVDIIEAGFPAASKD 128
           YIPN I DP+YVR+FDTTLRDGEQSPGA++TSKEKLDIARQLAKLGVDIIEAGFPAASKD
Sbjct: 78  YIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKD 137

Query: 129 DFEAVKMIAQEVGNAVDDEGYVPVICGLSRCNEKDIRTAWDAVKHAKRPRIHTFIATSGI 188
           DFEAVK IA+ VGN VD+ GYVPVICGLSRCN+KDI  AWDAVK+AKRPRIHTFIATS I
Sbjct: 138 DFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIATSDI 197

Query: 189 HMEYKLRMSKEQVIDKARSMVKFARSLGCNDVEFSPEDAGRSEREFLYEILAEVIKAGAT 248
           H+EYKL+ +K +VI+ ARSMV+FARSLGC DVEFSPEDAGRSERE+LYEIL EVIKAGAT
Sbjct: 198 HLEYKLKKTKAEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGAT 257

Query: 249 TLNIPDTVGITMPSEFGKLIADIKANTPGAENVVISTHCQNDLGLSTANTIEGAHAGARQ 308
           TLNIPDTVGIT+PSEFG+LI D+KANTPG ENVVISTHCQNDLGLSTANT+ GAHAGARQ
Sbjct: 258 TLNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAGARQ 317

Query: 309 LEVTINGIGERAGNASLEEVVMALKC-GGHVFGNLYTGVNAKHIYLTSRMVEEYSGLQLQ 367
           +EVTINGIGERAGNASLEEVVMA+KC G HV G L+TG++ +HI +TS+MVEEY+G+Q Q
Sbjct: 318 MEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQ 377

Query: 368 PHKALVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGLERSNEAGIVLGKLSGRHALRK 427
           PHKA+VGANAFAHESGIHQDGMLKHKGTYEII PE+IGLERSN+AGIVLGKLSGRHAL+ 
Sbjct: 378 PHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALKD 437

Query: 428 RLQELGYELKDDQVESLFWRFKAVAEQKKRVTDADLRALVSDEVFQAEPVWKLGDLQVTC 487
           RL ELGY+L D+Q+ ++FWRFK VAEQKKRVTDAD+ ALVSDEVFQ E VWKL D+Q+TC
Sbjct: 438 RLTELGYQLDDEQLSTIFWRFKTVAEQKKRVTDADIIALVSDEVFQPEAVWKLLDIQITC 497

Query: 488 GTLGLSTATVKLVSDDGSTHVACAIGTGPVDSAYKAVDLIVKEPITLLEYSMNAVTEGID 547
           GTLGLSTATVKL   DG  HVAC+IGTGPVDSAYKAVDLIVKEP TLLEYSMNAVTEGID
Sbjct: 498 GTLGLSTATVKLADADGKEHVACSIGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGID 557

Query: 548 AIATTRVVIRGETDHTSTHALTGETVHRTFGGSGAGMDXXXXXXXXXXXXINKMLGFKDT 607
           AIATTRV+IRG   ++ST+A+TGE V RTF G+GAGMD            +NKM+ FK+ 
Sbjct: 558 AIATTRVLIRGSNKYSSTNAITGEEVQRTFSGTGAGMDIVVSSVKAYVGALNKMMDFKEN 617

Query: 608 SPSTEKIPVSASKV 621
           S +  KIP   ++V
Sbjct: 618 SAT--KIPSQKNRV 629


>AT5G23010.1 | Symbols: MAM1, IMS3 | methylthioalkylmalate synthase
           1 | chr5:7703173-7706769 FORWARD LENGTH=506
          Length = 506

 Score =  545 bits (1403), Expect = e-155,   Method: Compositional matrix adjust.
 Identities = 255/433 (58%), Positives = 338/433 (78%), Gaps = 5/433 (1%)

Query: 43  CSSSSYPKFTVSCSQFQPETTRRRPPYIPNHIPDPSYVRIFDTTLRDGEQSPGASMTSKE 102
           C SS       S +  +P    R P YIPN +PD +YVR+FDTTLRDGEQSPG S+T  +
Sbjct: 49  CCSSVSKNAATSSTDLKP-VVERWPEYIPNKLPDGNYVRVFDTTLRDGEQSPGGSLTPPQ 107

Query: 103 KLDIARQLAKLGVDIIEAGFPAASKDDFEAVKMIAQEVGNAVDDE-GYVPVICGLSRCNE 161
           KL+IARQLAKL VDI+E GFP +S+++ E +K IA+ VGN VD+E GYVPVIC ++RC  
Sbjct: 108 KLEIARQLAKLRVDIMEVGFPGSSEEELETIKTIAKTVGNEVDEETGYVPVICAIARCKH 167

Query: 162 KDIRTAWDAVKHAKRPRIHTFIATSGIHMEYKLRMSKEQVIDKARSMVKFARSLGCNDVE 221
           +DI   W+A+K+AKRPRI  F +TS IHM+YKL+ ++E+VI+ A S ++FA+SLG ND++
Sbjct: 168 RDIEATWEALKYAKRPRILVFTSTSDIHMKYKLKKTQEEVIEMAVSSIRFAKSLGFNDIQ 227

Query: 222 FSPEDAGRSEREFLYEILAEVIKAGATTLNIPDTVGITMPSEFGKLIADIKANTPGAENV 281
           F  ED GRS+++FL +IL E IKAG T + I DTVGI MP E+G+L+  +KANTPG ++V
Sbjct: 228 FGCEDGGRSDKDFLCKILGEAIKAGVTVVTIGDTVGINMPHEYGELVTYLKANTPGIDDV 287

Query: 282 VISTHCQNDLGLSTANTIEGAHAGARQLEVTINGIGERAGNASLEEVVMALKC-GGHVFG 340
           V++ HC NDLGL+TAN+I G  AGARQ+EVTINGIGER+GNASLEEVVMALKC G +V  
Sbjct: 288 VVAVHCHNDLGLATANSIAGIRAGARQVEVTINGIGERSGNASLEEVVMALKCRGAYVIN 347

Query: 341 NLYTGVNAKHIYLTSRMVEEYSGLQLQPHKALVGANAFAHESGIHQDGMLKHKGTYEIIS 400
            +YT ++ + I  TS+MV+EY+GL +Q HK +VGAN F HESGIHQDG+LK++ TYEI+S
Sbjct: 348 GVYTKIDTRQIMATSKMVQEYTGLYVQAHKPIVGANCFVHESGIHQDGILKNRSTYEILS 407

Query: 401 PEDIGLERSNEAGIVLGKLSGRHALRKRLQELGYELKDDQVESLFWRFKAVAEQKKRVTD 460
           PEDIG+ +S  +G+VLGKLSGRHA++ RL+ELGYEL D+++ ++F  F+ + + KKR+TD
Sbjct: 408 PEDIGIVKSQNSGLVLGKLSGRHAVKDRLKELGYELDDEKLNAVFSLFRDLTKNKKRITD 467

Query: 461 ADLRALV--SDEV 471
           ADL+ALV  SDE+
Sbjct: 468 ADLKALVTSSDEI 480


>AT5G23020.1 | Symbols: MAM-L, MAM3, IMS2 | 2-isopropylmalate
           synthase 2 | chr5:7718203-7721634 FORWARD LENGTH=503
          Length = 503

 Score =  539 bits (1389), Expect = e-153,   Method: Compositional matrix adjust.
 Identities = 248/427 (58%), Positives = 336/427 (78%), Gaps = 3/427 (0%)

Query: 43  CSSSSYPKFTVSCSQFQPETTRRRPPYIPNHIPDPSYVRIFDTTLRDGEQSPGASMTSKE 102
           C S+   K   S +  +P    RRP YIPN +P  +YVR+ DTTLRDGEQSPGA++T  +
Sbjct: 49  CCSAESKKVATSATDLKP-IMERRPEYIPNKLPHKNYVRVLDTTLRDGEQSPGAALTPPQ 107

Query: 103 KLDIARQLAKLGVDIIEAGFPAASKDDFEAVKMIAQEVGNAVDDE-GYVPVICGLSRCNE 161
           KL+IARQLAKL VDI+E GFP +S+++FEA+K IA+ VGN VD+E GYVPVICG++RC +
Sbjct: 108 KLEIARQLAKLRVDIMEVGFPVSSEEEFEAIKTIAKTVGNEVDEETGYVPVICGIARCKK 167

Query: 162 KDIRTAWDAVKHAKRPRIHTFIATSGIHMEYKLRMSKEQVIDKARSMVKFARSLGCNDVE 221
           +DI   W+A+K+AKRPR+  F +TS IHM+YKL+ +KE+VI+ A + VK+A+SLG  D++
Sbjct: 168 RDIEATWEALKYAKRPRVMLFTSTSEIHMKYKLKKTKEEVIEMAVNSVKYAKSLGFKDIQ 227

Query: 222 FSPEDAGRSEREFLYEILAEVIKAGATTLNIPDTVGITMPSEFGKLIADIKANTPGAENV 281
           F  ED GR+E++F+ +IL E IKAGATT+   DTVGI MP EFG+L+A +  NTPGA+++
Sbjct: 228 FGCEDGGRTEKDFICKILGESIKAGATTVGFADTVGINMPQEFGELVAYVIENTPGADDI 287

Query: 282 VISTHCQNDLGLSTANTIEGAHAGARQLEVTINGIGERAGNASLEEVVMALKCGGH-VFG 340
           V + HC NDLG++TANTI G  AGARQ+EVTINGIGER+GNA LEEVVMALKC G  +  
Sbjct: 288 VFAIHCHNDLGVATANTISGICAGARQVEVTINGIGERSGNAPLEEVVMALKCRGESLMD 347

Query: 341 NLYTGVNAKHIYLTSRMVEEYSGLQLQPHKALVGANAFAHESGIHQDGMLKHKGTYEIIS 400
            +YT ++++ I  TS+MV+E++G+ +QPHK +VG N F HESGIHQDG+LK++ TYEI+S
Sbjct: 348 GVYTKIDSRQIMATSKMVQEHTGMYVQPHKPIVGDNCFVHESGIHQDGILKNRSTYEILS 407

Query: 401 PEDIGLERSNEAGIVLGKLSGRHALRKRLQELGYELKDDQVESLFWRFKAVAEQKKRVTD 460
           PED+G+ +S  +GIVLGKLSGRHA++ RL+ELGYE+ D++   +F R++ + + KKR+TD
Sbjct: 408 PEDVGIVKSENSGIVLGKLSGRHAVKDRLKELGYEISDEKFNDIFSRYRELTKDKKRITD 467

Query: 461 ADLRALV 467
           ADL+ALV
Sbjct: 468 ADLKALV 474