Miyakogusa Predicted Gene

Lj5g3v2240910.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v2240910.1 Non Chatacterized Hit- tr|D7TXX5|D7TXX5_VITVI
Putative uncharacterized protein OS=Vitis vinifera
GN=,92.81,0,Radical_SAM,Radical SAM; Elongator protein 3, MiaB family,
Radical SA,Elongator protein 3/MiaB/NifB;,CUFF.57005.1
         (340 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G08415.1 | Symbols:  | Radical SAM superfamily protein | chr5...   546   e-156
AT2G20860.1 | Symbols: LIP1 | lipoic acid synthase 1 | chr2:8979...   352   2e-97

>AT5G08415.1 | Symbols:  | Radical SAM superfamily protein |
           chr5:2710983-2713101 REVERSE LENGTH=394
          Length = 394

 Score =  546 bits (1406), Expect = e-156,   Method: Compositional matrix adjust.
 Identities = 265/294 (90%), Positives = 280/294 (95%)

Query: 47  LGPYTGRDPNVKKPEWLRQKAPQGERFQQIKDSLSHLKLNTVCEEAQCPNIGECWNGGGD 106
           +GP+TGRDPNVKKP WLRQKAPQGERFQ++K+SLS L LNTVCEEAQCPNIGECWNGGGD
Sbjct: 85  MGPFTGRDPNVKKPAWLRQKAPQGERFQEVKESLSRLNLNTVCEEAQCPNIGECWNGGGD 144

Query: 107 GIATATIMLLGDTCTRGCRFCAVKTSRNPAPPDPMEPENTANAIASWDVDYIVLTSVDRD 166
           G+ATATIM+LGDTCTRGCRFCAVKTSRNP PPDPMEPENTA AIASW VDYIV+TSVDRD
Sbjct: 145 GVATATIMVLGDTCTRGCRFCAVKTSRNPPPPDPMEPENTAKAIASWGVDYIVITSVDRD 204

Query: 167 DIPDGGSGHFAQTVKAMKNLKPEIMVECLTSDFRGDLTAVETLVHSGLDVFAHNVETVKR 226
           DIPDGGSGHFAQTVKAMK  KP+IM+ECLTSDFRGDL AV+TLVHSGLDVFAHNVETVKR
Sbjct: 205 DIPDGGSGHFAQTVKAMKRHKPDIMIECLTSDFRGDLEAVDTLVHSGLDVFAHNVETVKR 264

Query: 227 LQRIVRDPRAGYEQSLSVLKHAKHSKEGMITKTSIMLGLGESDDELKEVMADLRAIDVDI 286
           LQR+VRDPRAGYEQS+SVLKHAK SK GMITKTSIMLGLGE+D+ELKE MADLRAIDVDI
Sbjct: 265 LQRLVRDPRAGYEQSMSVLKHAKISKPGMITKTSIMLGLGETDEELKEAMADLRAIDVDI 324

Query: 287 LTLGQYLQPTPLHLTVKEYVTPEKFAFWKEYGESIGFRYVASGPLVRSSYRAGE 340
           LTLGQYLQPTPLHLTVKEYVTPEKF FWK YGESIGFRYVASGPLVRSSYRAGE
Sbjct: 325 LTLGQYLQPTPLHLTVKEYVTPEKFDFWKTYGESIGFRYVASGPLVRSSYRAGE 378


>AT2G20860.1 | Symbols: LIP1 | lipoic acid synthase 1 |
           chr2:8979636-8980983 FORWARD LENGTH=374
          Length = 374

 Score =  352 bits (902), Expect = 2e-97,   Method: Compositional matrix adjust.
 Identities = 172/280 (61%), Positives = 217/280 (77%), Gaps = 1/280 (0%)

Query: 62  WLRQKAPQGERFQQIKDSLSHLKLNTVCEEAQCPNIGECWNGGGDGIATATIMLLGDTCT 121
           W+++  P GER+ QIK  L  LKL+TVCEEA+CPN+GECW+GG  G ATATIM+LGDTCT
Sbjct: 76  WMKESIPGGERYVQIKKKLRDLKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCT 135

Query: 122 RGCRFCAVKTSRNPAPPDPMEPENTANAIASWDVDYIVLTSVDRDDIPDGGSGHFAQTVK 181
           RGCRFC VKTSR P PPDP EP N A AIASW VDY+V+TSVDRDD+PD GSGHFA+TV+
Sbjct: 136 RGCRFCNVKTSRTPPPPDPNEPNNVAEAIASWGVDYVVITSVDRDDLPDQGSGHFAETVQ 195

Query: 182 AMKNLKPEIMVECLTSDFRGDLTAVETLVHSGLDVFAHNVETVKRLQRIVRDPRAGYEQS 241
            +K LKPE+++E L  DFRGD   VE +  SGLDV AHN+ETV+ LQ  VRD RA ++QS
Sbjct: 196 RLKFLKPEMLIEALVPDFRGDGGCVEKVSKSGLDVLAHNIETVEELQSFVRDHRANFKQS 255

Query: 242 LSVLKHAK-HSKEGMITKTSIMLGLGESDDELKEVMADLRAIDVDILTLGQYLQPTPLHL 300
           L VL+ AK ++  G +TKTS+MLG GE+ D++ + M  +RA  VD++T GQY++P+  H+
Sbjct: 256 LDVLRMAKEYAPAGTLTKTSVMLGCGETPDQVVKTMEKVRAAGVDVMTFGQYMRPSKRHM 315

Query: 301 TVKEYVTPEKFAFWKEYGESIGFRYVASGPLVRSSYRAGE 340
            V EYVTP+ F  ++  G  +GFRYVASGP+VRSSY+AGE
Sbjct: 316 PVAEYVTPDAFERYRLLGMEMGFRYVASGPMVRSSYKAGE 355