Miyakogusa Predicted Gene

Lj5g3v1889400.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v1889400.1 Non Chatacterized Hit- tr|I3S4G2|I3S4G2_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2
SV=1,99.22,0,FA_hydroxylase,Fatty acid hydroxylase; seg,NULL; SUR2
HYDROXYLASE/DESATURASE,NULL; STEROL DESATURASE,CUFF.56185.1
         (257 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G69640.1 | Symbols: SBH1 | sphingoid base hydroxylase 1 | chr...   324   4e-89
AT1G14290.1 | Symbols: SBH2 | sphingoid base hydroxylase 2 | chr...   322   2e-88
AT2G29390.4 | Symbols: ATSMO2 | sterol 4-alpha-methyl-oxidase 2-...    67   1e-11
AT2G29390.2 | Symbols: ATSMO2 | sterol 4-alpha-methyl-oxidase 2-...    66   2e-11
AT2G29390.5 | Symbols: ATSMO2 | sterol 4-alpha-methyl-oxidase 2-...    66   2e-11
AT2G29390.3 | Symbols: ATSMO2 | sterol 4-alpha-methyl-oxidase 2-...    66   3e-11
AT2G29390.1 | Symbols: SMO2-2, ATSMO2 | sterol 4-alpha-methyl-ox...    65   5e-11
AT1G07420.1 | Symbols: ATSMO2, SMO2-1 | sterol 4-alpha-methyl-ox...    64   8e-11
AT1G07420.2 | Symbols: ATSMO2, SMO2-1 | sterol 4-alpha-methyl-ox...    64   1e-10
AT4G12110.1 | Symbols: SMO1-1, ATSMO1, ATSMO1-1 | sterol-4alpha-...    56   2e-08
AT4G22753.1 | Symbols: SMO1-3, ATSMO1, SMO1, ATSMO1-3 | sterol 4...    54   9e-08
AT4G22753.2 | Symbols: SMO1-3 | sterol 4-alpha methyl oxidase 1-...    54   1e-07
AT4G22756.1 | Symbols: SMO1-2, ATSMO1, ATSMO1-2, SMO1 | sterol C...    52   4e-07

>AT1G69640.1 | Symbols: SBH1 | sphingoid base hydroxylase 1 |
           chr1:26193933-26195466 REVERSE LENGTH=260
          Length = 260

 Score =  324 bits (830), Expect = 4e-89,   Method: Compositional matrix adjust.
 Identities = 148/250 (59%), Positives = 188/250 (75%)

Query: 8   VSDEVMGTFAPIVVYWVYAGFYHLLPPLDNFRLHTQRDEEKKNLVPFKAXXXXXXXXXXX 67
           VSDE++GT APIVVYW+Y+G Y  L  L+++RLH++ +EE+KNLV   +           
Sbjct: 7   VSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLVQQVV 66

Query: 68  XXXXXXXXXXXXXNANASGVIVQPSITKQALQILIAMFVMDSWQYFVHRYMHQNKFLYRH 127
                        +   +    Q S    A Q + AM V+D+WQYF+HRYMHQNKFLY+H
Sbjct: 67  QAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKFLYKH 126

Query: 128 IHSQHHRLVVPYAIGALYNHPMEGLLLDTVGGAISFLVSGMTARTAVVFFCFAVVKTVDD 187
           IHSQHHRL+VPYA GALYNHP+EGLLLDT+GGA+SFLVSGM+ RT++ FF FA +KTVDD
Sbjct: 127 IHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIKTVDD 186

Query: 188 HCGLWLPGNIFHLFFQNNTAYHDIHHQLQGLKYNYSQPFFPIWDKLLGTHMPFNLVKRPE 247
           HCGLWLPGN+FH+ F+NN+AYHDIHHQL G KYN+SQPFF +WD++LGT+MP++L KR +
Sbjct: 187 HCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLEKRED 246

Query: 248 GGFEARPAKD 257
           GGFEARP K+
Sbjct: 247 GGFEARPTKE 256


>AT1G14290.1 | Symbols: SBH2 | sphingoid base hydroxylase 2 |
           chr1:4880307-4881784 REVERSE LENGTH=259
          Length = 259

 Score =  322 bits (825), Expect = 2e-88,   Method: Compositional matrix adjust.
 Identities = 147/249 (59%), Positives = 182/249 (73%)

Query: 8   VSDEVMGTFAPIVVYWVYAGFYHLLPPLDNFRLHTQRDEEKKNLVPFKAXXXXXXXXXXX 67
           +SDE +GTF PI+VYWVY+G Y  L  LD +RLH++ DE++KNLV   A           
Sbjct: 6   ISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLLQQTL 65

Query: 68  XXXXXXXXXXXXXNANASGVIVQPSITKQALQILIAMFVMDSWQYFVHRYMHQNKFLYRH 127
                        +   +    Q SI   A Q +IAM V+D+WQYF+HRYMH NKFLY+H
Sbjct: 66  QAIISVILFKITGSDADAATTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNKFLYKH 125

Query: 128 IHSQHHRLVVPYAIGALYNHPMEGLLLDTVGGAISFLVSGMTARTAVVFFCFAVVKTVDD 187
           IHSQHHRL+VPY+ GALYNHP+EGLLLDT+GGA+SFL SGM+ RTA+ FF FA +KTVDD
Sbjct: 126 IHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFATIKTVDD 185

Query: 188 HCGLWLPGNIFHLFFQNNTAYHDIHHQLQGLKYNYSQPFFPIWDKLLGTHMPFNLVKRPE 247
           HCGLWLPGN FH+FF NN+AYHD+HHQL G KYN+SQPFF +WD++LGT++P++L KR  
Sbjct: 186 HCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWDRILGTYLPYSLEKRAN 245

Query: 248 GGFEARPAK 256
           GGFE RP K
Sbjct: 246 GGFETRPIK 254


>AT2G29390.4 | Symbols: ATSMO2 | sterol 4-alpha-methyl-oxidase 2-2 |
           chr2:12610758-12611731 REVERSE LENGTH=177
          Length = 177

 Score = 67.0 bits (162), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 53/161 (32%), Positives = 78/161 (48%), Gaps = 15/161 (9%)

Query: 91  PSITKQALQILIAMFVMDSWQYFVHRYMHQNKFLYRHIHSQHHRLVVPYAIGALYNHPME 150
           PS    + QIL    + D   Y+ HR +H  K+LY+++HS HH    P+ + + Y HP E
Sbjct: 20  PSWKVVSAQILFYFIIEDFVFYWGHRILH-TKWLYKNVHSVHHEYATPFGLTSEYAHPAE 78

Query: 151 GLLL---DTVGGAISFLVSGMTARTAVVFFCFAVVKTVDDHCGL---WLPGNIFHLFFQN 204
            L L     VG A+    +G    T  ++    V++TV+ HCG    W P N   L+   
Sbjct: 79  ILFLGFATIVGPAL----TGPHLITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLY--G 132

Query: 205 NTAYHDIHHQLQGLKY-NYSQPFFPIWDKLLGTHMPFNLVK 244
              +HD HH+L   K  NYS  F  + D + GT   +  +K
Sbjct: 133 GADFHDYHHRLLYTKSGNYSSTFVYM-DWIFGTDKGYRKLK 172


>AT2G29390.2 | Symbols: ATSMO2 | sterol 4-alpha-methyl-oxidase 2-2 |
           chr2:12610758-12612799 REVERSE LENGTH=260
          Length = 260

 Score = 66.2 bits (160), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 53/161 (32%), Positives = 78/161 (48%), Gaps = 15/161 (9%)

Query: 91  PSITKQALQILIAMFVMDSWQYFVHRYMHQNKFLYRHIHSQHHRLVVPYAIGALYNHPME 150
           PS    + QIL    + D   Y+ HR +H  K+LY+++HS HH    P+ + + Y HP E
Sbjct: 103 PSWKVVSAQILFYFIIEDFVFYWGHRILH-TKWLYKNVHSVHHEYATPFGLTSEYAHPAE 161

Query: 151 GLLL---DTVGGAISFLVSGMTARTAVVFFCFAVVKTVDDHCGL---WLPGNIFHLFFQN 204
            L L     VG A+    +G    T  ++    V++TV+ HCG    W P N   L+   
Sbjct: 162 ILFLGFATIVGPAL----TGPHLITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLY--G 215

Query: 205 NTAYHDIHHQLQGLKY-NYSQPFFPIWDKLLGTHMPFNLVK 244
              +HD HH+L   K  NYS  F  + D + GT   +  +K
Sbjct: 216 GADFHDYHHRLLYTKSGNYSSTFVYM-DWIFGTDKGYRKLK 255


>AT2G29390.5 | Symbols: ATSMO2 | sterol 4-alpha-methyl-oxidase 2-2 |
           chr2:12610758-12611929 REVERSE LENGTH=219
          Length = 219

 Score = 66.2 bits (160), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 53/161 (32%), Positives = 78/161 (48%), Gaps = 15/161 (9%)

Query: 91  PSITKQALQILIAMFVMDSWQYFVHRYMHQNKFLYRHIHSQHHRLVVPYAIGALYNHPME 150
           PS    + QIL    + D   Y+ HR +H  K+LY+++HS HH    P+ + + Y HP E
Sbjct: 62  PSWKVVSAQILFYFIIEDFVFYWGHRILH-TKWLYKNVHSVHHEYATPFGLTSEYAHPAE 120

Query: 151 GLLL---DTVGGAISFLVSGMTARTAVVFFCFAVVKTVDDHCGL---WLPGNIFHLFFQN 204
            L L     VG A+    +G    T  ++    V++TV+ HCG    W P N   L+   
Sbjct: 121 ILFLGFATIVGPAL----TGPHLITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLY--G 174

Query: 205 NTAYHDIHHQLQGLKY-NYSQPFFPIWDKLLGTHMPFNLVK 244
              +HD HH+L   K  NYS  F  + D + GT   +  +K
Sbjct: 175 GADFHDYHHRLLYTKSGNYSSTFVYM-DWIFGTDKGYRKLK 214


>AT2G29390.3 | Symbols: ATSMO2 | sterol 4-alpha-methyl-oxidase 2-2 |
           chr2:12610758-12612149 REVERSE LENGTH=253
          Length = 253

 Score = 65.9 bits (159), Expect = 3e-11,   Method: Compositional matrix adjust.
 Identities = 53/161 (32%), Positives = 78/161 (48%), Gaps = 15/161 (9%)

Query: 91  PSITKQALQILIAMFVMDSWQYFVHRYMHQNKFLYRHIHSQHHRLVVPYAIGALYNHPME 150
           PS    + QIL    + D   Y+ HR +H  K+LY+++HS HH    P+ + + Y HP E
Sbjct: 96  PSWKVVSAQILFYFIIEDFVFYWGHRILH-TKWLYKNVHSVHHEYATPFGLTSEYAHPAE 154

Query: 151 GLLL---DTVGGAISFLVSGMTARTAVVFFCFAVVKTVDDHCGL---WLPGNIFHLFFQN 204
            L L     VG A+    +G    T  ++    V++TV+ HCG    W P N   L+   
Sbjct: 155 ILFLGFATIVGPAL----TGPHLITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLY--G 208

Query: 205 NTAYHDIHHQLQGLKY-NYSQPFFPIWDKLLGTHMPFNLVK 244
              +HD HH+L   K  NYS  F  + D + GT   +  +K
Sbjct: 209 GADFHDYHHRLLYTKSGNYSSTFVYM-DWIFGTDKGYRKLK 248


>AT2G29390.1 | Symbols: SMO2-2, ATSMO2 | sterol
           4-alpha-methyl-oxidase 2-2 | chr2:12610758-12612799
           REVERSE LENGTH=272
          Length = 272

 Score = 64.7 bits (156), Expect = 5e-11,   Method: Compositional matrix adjust.
 Identities = 54/171 (31%), Positives = 80/171 (46%), Gaps = 23/171 (13%)

Query: 91  PSITKQALQILIAMFVMDSWQYFVHRYMHQNKFLYRHIHSQHHRLVVPYAIGALYNHPME 150
           PS    + QIL    + D   Y+ HR +H  K+LY+++HS HH    P+ + + Y HP E
Sbjct: 103 PSWKVVSAQILFYFIIEDFVFYWGHRILH-TKWLYKNVHSVHHEYATPFGLTSEYAHPAE 161

Query: 151 GLLL---DTVGGAISFLVSGMTARTAVVFFCFAVVKTVDDHCGL---WLPGNIFHLF--- 201
            L L     VG A+    +G    T  ++    V++TV+ HCG    W P N   L+   
Sbjct: 162 ILFLGFATIVGPAL----TGPHLITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGS 217

Query: 202 -------FQNNTAYHDIHHQLQGLKY-NYSQPFFPIWDKLLGTHMPFNLVK 244
                  F  +  +HD HH+L   K  NYS  F  + D + GT   +  +K
Sbjct: 218 LILMWESFAYSADFHDYHHRLLYTKSGNYSSTFVYM-DWIFGTDKGYRKLK 267


>AT1G07420.1 | Symbols: ATSMO2, SMO2-1 | sterol
           4-alpha-methyl-oxidase 2-1 | chr1:2277910-2280033
           FORWARD LENGTH=266
          Length = 266

 Score = 64.3 bits (155), Expect = 8e-11,   Method: Compositional matrix adjust.
 Identities = 50/159 (31%), Positives = 79/159 (49%), Gaps = 11/159 (6%)

Query: 91  PSITKQALQILIAMFVMDSWQYFVHRYMHQNKFLYRHIHSQHHRLVVPYAIGALYNHPME 150
           PS  + + QIL    + D   Y+ HR +H +K+LY+++HS HH    P+ + + Y HP E
Sbjct: 103 PSWKEVSAQILFYFIIEDFVFYWGHRILH-SKWLYKNVHSVHHEYATPFGLTSEYAHPAE 161

Query: 151 GLLL---DTVGGAISFLVSGMTARTAVVFFCFAVVKTVDDHCGLWLPGNIFHLF-FQNNT 206
            L L     VG A+    +G    T  ++    V++TV+ HCG   P ++ +        
Sbjct: 162 ILFLGFATIVGPAL----TGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGA 217

Query: 207 AYHDIHHQLQGLKY-NYSQPFFPIWDKLLGTHMPFNLVK 244
            +HD HH+L   K  NYS  F  + D + GT   +  +K
Sbjct: 218 DFHDYHHRLLYTKSGNYSSTFVYM-DWIFGTDKGYRRLK 255


>AT1G07420.2 | Symbols: ATSMO2, SMO2-1 | sterol
           4-alpha-methyl-oxidase 2-1 | chr1:2278918-2280033
           FORWARD LENGTH=228
          Length = 228

 Score = 63.9 bits (154), Expect = 1e-10,   Method: Compositional matrix adjust.
 Identities = 50/159 (31%), Positives = 79/159 (49%), Gaps = 11/159 (6%)

Query: 91  PSITKQALQILIAMFVMDSWQYFVHRYMHQNKFLYRHIHSQHHRLVVPYAIGALYNHPME 150
           PS  + + QIL    + D   Y+ HR +H +K+LY+++HS HH    P+ + + Y HP E
Sbjct: 65  PSWKEVSAQILFYFIIEDFVFYWGHRILH-SKWLYKNVHSVHHEYATPFGLTSEYAHPAE 123

Query: 151 GLLL---DTVGGAISFLVSGMTARTAVVFFCFAVVKTVDDHCGLWLPGNIFHLF-FQNNT 206
            L L     VG A+    +G    T  ++    V++TV+ HCG   P ++ +        
Sbjct: 124 ILFLGFATIVGPAL----TGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGA 179

Query: 207 AYHDIHHQLQGLKY-NYSQPFFPIWDKLLGTHMPFNLVK 244
            +HD HH+L   K  NYS  F  + D + GT   +  +K
Sbjct: 180 DFHDYHHRLLYTKSGNYSSTFVYM-DWIFGTDKGYRRLK 217


>AT4G12110.1 | Symbols: SMO1-1, ATSMO1, ATSMO1-1 |
           sterol-4alpha-methyl oxidase 1-1 | chr4:7254197-7256004
           FORWARD LENGTH=298
          Length = 298

 Score = 56.2 bits (134), Expect = 2e-08,   Method: Compositional matrix adjust.
 Identities = 43/161 (26%), Positives = 70/161 (43%), Gaps = 13/161 (8%)

Query: 91  PSITKQALQILIAMFVMDSWQYFVHRYMHQNKFLYRHIHSQHHRLVVPYAIGALYNHPME 150
           P+IT+   Q+++   + D   Y+VHR+ H +K+ Y  IH  HH    P    A Y H  E
Sbjct: 123 PTITEMLSQLVVYFLIEDYTNYWVHRFFH-SKWGYDKIHRVHHEYTAPIGYAAPYAHWAE 181

Query: 151 GLLLDTVGGAISFLVSGMTARTAVVFFCFAVVK---TVDDHCGL---WLPGNIFHLFFQN 204
            LLL    G  +F+   +     + F+ +  ++    ++ H G    W P    ++ F  
Sbjct: 182 VLLL----GIPTFMGPAIAPGHMITFWLWIALRQMEAIETHSGYDFPWSPTK--YIPFYG 235

Query: 205 NTAYHDIHHQLQGLKYNYSQPFFPIWDKLLGTHMPFNLVKR 245
              YHD HH + G   +     F   D + GT   +   K+
Sbjct: 236 GAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKK 276


>AT4G22753.1 | Symbols: SMO1-3, ATSMO1, SMO1, ATSMO1-3 | sterol
           4-alpha methyl oxidase 1-3 | chr4:11952019-11953609
           REVERSE LENGTH=291
          Length = 291

 Score = 53.9 bits (128), Expect = 9e-08,   Method: Compositional matrix adjust.
 Identities = 41/159 (25%), Positives = 70/159 (44%), Gaps = 9/159 (5%)

Query: 91  PSITKQALQILIAMFVMDSWQYFVHRYMHQNKFLYRHIHSQHHRLVVPYAIGALYNHPME 150
           PS+ +   Q+++   + D   Y++HR+MH  K+ Y  IH  HH    P    + Y H  E
Sbjct: 119 PSLMEIVAQLVVYFLIEDYTNYWIHRWMH-CKWGYEKIHRIHHEYTSPIGYASPYAHWAE 177

Query: 151 GLLLDTVGGAISFLVSGMTARTAVVFFCFAVVK---TVDDHCGLWLPGNIFHLF-FQNNT 206
            L+L    G  +FL   +     + F+ +  ++    ++ H G   P ++  L  F    
Sbjct: 178 ILIL----GIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGP 233

Query: 207 AYHDIHHQLQGLKYNYSQPFFPIWDKLLGTHMPFNLVKR 245
            YHD HH + G   +     F   D + GT   + + K+
Sbjct: 234 EYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKK 272


>AT4G22753.2 | Symbols: SMO1-3 | sterol 4-alpha methyl oxidase 1-3 |
           chr4:11949366-11953609 REVERSE LENGTH=581
          Length = 581

 Score = 53.9 bits (128), Expect = 1e-07,   Method: Compositional matrix adjust.
 Identities = 41/159 (25%), Positives = 70/159 (44%), Gaps = 9/159 (5%)

Query: 91  PSITKQALQILIAMFVMDSWQYFVHRYMHQNKFLYRHIHSQHHRLVVPYAIGALYNHPME 150
           PS+ +   Q+++   + D   Y++HR+MH  K+ Y  IH  HH    P    + Y H  E
Sbjct: 119 PSLMEIVAQLVVYFLIEDYTNYWIHRWMH-CKWGYEKIHRIHHEYTSPIGYASPYAHWAE 177

Query: 151 GLLLDTVGGAISFLVSGMTARTAVVFFCFAVVK---TVDDHCGLWLPGNIFHLF-FQNNT 206
            L+L    G  +FL   +     + F+ +  ++    ++ H G   P ++  L  F    
Sbjct: 178 ILIL----GIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGP 233

Query: 207 AYHDIHHQLQGLKYNYSQPFFPIWDKLLGTHMPFNLVKR 245
            YHD HH + G   +     F   D + GT   + + K+
Sbjct: 234 EYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKK 272


>AT4G22756.1 | Symbols: SMO1-2, ATSMO1, ATSMO1-2, SMO1 | sterol
           C4-methyl oxidase 1-2 | chr4:11955124-11956695 REVERSE
           LENGTH=299
          Length = 299

 Score = 52.0 bits (123), Expect = 4e-07,   Method: Compositional matrix adjust.
 Identities = 42/159 (26%), Positives = 67/159 (42%), Gaps = 9/159 (5%)

Query: 91  PSITKQALQILIAMFVMDSWQYFVHRYMHQNKFLYRHIHSQHHRLVVPYAIGALYNHPME 150
           PS  +   Q+++   V D   Y+VHR+ H  K+ Y   H  HH    P    A Y H  E
Sbjct: 123 PSCMEIVAQLVVYFLVEDYTNYWVHRFFH-CKWGYEKFHHIHHEYTAPIGYAAPYAHWAE 181

Query: 151 GLLLDTVGGAISFLVSGMTARTAVVFFCFAV---VKTVDDHCGLWLPGNIF-HLFFQNNT 206
            LLL    G  +FL   +     + F+ +     ++ ++ H G   P ++  ++ F    
Sbjct: 182 VLLL----GIPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKYIPFYGGA 237

Query: 207 AYHDIHHQLQGLKYNYSQPFFPIWDKLLGTHMPFNLVKR 245
            YHD HH + G   +     F   D + GT   +   K+
Sbjct: 238 EYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKK 276