Miyakogusa Predicted Gene

Lj5g3v0709800.2

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v0709800.2 Non Chatacterized Hit- tr|I1LZF1|I1LZF1_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.27919 PE,86.72,0,C-14
STEROL REDUCTASE,NULL; STEROL REDUCTASE/LAMIN B RECEPTOR,NULL;
ERG4_ERG24,Ergosterol biosynthes,CUFF.53830.2
         (369 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G52940.1 | Symbols: FK, HYD2, ELL1 | Ergosterol biosynthesis ...   487   e-138
AT3G52940.2 | Symbols: FK, HYD2, ELL1 | Ergosterol biosynthesis ...   281   4e-76
AT1G50430.2 | Symbols: DWF5, PA, LE, ST7R, 7RED | Ergosterol bio...   126   2e-29
AT1G50430.1 | Symbols: DWF5, PA, LE, ST7R, 7RED | Ergosterol bio...   123   2e-28

>AT3G52940.1 | Symbols: FK, HYD2, ELL1 | Ergosterol biosynthesis
           ERG4/ERG24 family | chr3:19630369-19633112 REVERSE
           LENGTH=369
          Length = 369

 Score =  487 bits (1254), Expect = e-138,   Method: Compositional matrix adjust.
 Identities = 242/369 (65%), Positives = 263/369 (71%), Gaps = 4/369 (1%)

Query: 1   MDLGCPVHGLIPSWNSXXXXXXXXXXXXXXXSVVPGKLIAGVVLPDKSRLRYRCNGXXXX 60
           MDLG     L+PS  S                ++PGK+I GV+L D S+LRYRCNG    
Sbjct: 5   MDLGV----LLPSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGLLAL 60

Query: 61  XXXXXXXGIGAKMGFVSPTAISERGLELLSTTFIFSFLVALILYFXXXXXXXXXXXXXXX 120
                  GI AK+G VSP  +++RGLELLS TFIF  LV L LY                
Sbjct: 61  ILLVAILGICAKLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTGRSSSNKGSSLKPH 120

Query: 121 XXGNLIHDWWFGIQLNPQVMGIDLKFFFVRAGMMGWLLINLSVLAKSIQDDTLSQSMILF 180
             GNL+HDWWFGIQLNPQ M IDLKFFFVRAGMMGWLLINLS+LAKS+QD +LSQSMIL+
Sbjct: 121 VSGNLVHDWWFGIQLNPQFMSIDLKFFFVRAGMMGWLLINLSILAKSVQDGSLSQSMILY 180

Query: 181 QLFCGIYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLRNKVELT 240
           Q+FC +YILDYFVHEEYMTSTWDIIAERLGFMLVFGDL+WIPFTFSIQGWWLL NKVELT
Sbjct: 181 QIFCALYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFSIQGWWLLHNKVELT 240

Query: 241 TAAIVANCFVFLIGYMVFRGANKQKHEFKKNPKAPIWGKPPIVIGGKLLASGYWGVARHC 300
             AIV NC VFLIGYMVFRGANKQKH FKKNPK PIWGKPP+V+GGKLL SGYWG+ARHC
Sbjct: 241 VPAIVVNCLVFLIGYMVFRGANKQKHIFKKNPKTPIWGKPPVVVGGKLLVSGYWGIARHC 300

Query: 301 NYXXXXXXXXXXXXPCGISSXXXXXXXXXXXXXXXWRERRDEARCAEKYKEIWAEYRRTV 360
           NY            PCGISS               WRERRDE RCAEKYKEIWAEY R V
Sbjct: 301 NYLGDLMLALSFSLPCGISSPVPYFYPIYLLILLIWRERRDEVRCAEKYKEIWAEYLRLV 360

Query: 361 PWRILPYVY 369
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369


>AT3G52940.2 | Symbols: FK, HYD2, ELL1 | Ergosterol biosynthesis
           ERG4/ERG24 family | chr3:19631030-19633112 REVERSE
           LENGTH=262
          Length = 262

 Score =  281 bits (720), Expect = 4e-76,   Method: Compositional matrix adjust.
 Identities = 143/230 (62%), Positives = 161/230 (70%), Gaps = 4/230 (1%)

Query: 1   MDLGCPVHGLIPSWNSXXXXXXXXXXXXXXXSVVPGKLIAGVVLPDKSRLRYRCNGXXXX 60
           MDLG     L+PS  S                ++PGK+I GV+L D S+LRYRCNG    
Sbjct: 5   MDLGV----LLPSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGLLAL 60

Query: 61  XXXXXXXGIGAKMGFVSPTAISERGLELLSTTFIFSFLVALILYFXXXXXXXXXXXXXXX 120
                  GI AK+G VSP  +++RGLELLS TFIF  LV L LY                
Sbjct: 61  ILLVAILGICAKLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTGRSSSNKGSSLKPH 120

Query: 121 XXGNLIHDWWFGIQLNPQVMGIDLKFFFVRAGMMGWLLINLSVLAKSIQDDTLSQSMILF 180
             GNL+HDWWFGIQLNPQ M IDLKFFFVRAGMMGWLLINLS+LAKS+QD +LSQSMIL+
Sbjct: 121 VSGNLVHDWWFGIQLNPQFMSIDLKFFFVRAGMMGWLLINLSILAKSVQDGSLSQSMILY 180

Query: 181 QLFCGIYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGW 230
           Q+FC +YILDYFVHEEYMTSTWDIIAERLGFMLVFGDL+WIPFTFSIQ W
Sbjct: 181 QIFCALYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFSIQAW 230


>AT1G50430.2 | Symbols: DWF5, PA, LE, ST7R, 7RED | Ergosterol
           biosynthesis ERG4/ERG24 family | chr1:18682175-18685555
           REVERSE LENGTH=431
          Length = 431

 Score =  126 bits (316), Expect = 2e-29,   Method: Compositional matrix adjust.
 Identities = 92/353 (26%), Positives = 158/353 (44%), Gaps = 20/353 (5%)

Query: 33  VVPGKLIAGVVLPDKSRLRYRCNGXXXXXXXXXXXGIGAKMGFVSPTAISERGLELLSTT 92
           ++PGK + G + P  +R  Y+ NG            +G   G  +P  + +   E+ S  
Sbjct: 83  LLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATY-LGLWFGIFNPAIVYDHLGEIFSAL 141

Query: 93  FIFSFLVALILYFXXXXXXXXXXXXXXXXXGNLIHDWWFGIQLNPQV-MGIDLKFFF-VR 150
              SF+  ++LY                  GNLI D+++G++L P++    D+K F   R
Sbjct: 142 IFGSFIFCVLLYIKGHVAPSSSDSGSC---GNLIIDFYWGMELYPRIGKSFDIKVFTNCR 198

Query: 151 AGMMGWLLINLSVLAKSIQ-DDTLSQSMILFQLFCGIYILDYFVHEEYMTSTWDIIAERL 209
            GMM W ++ ++   K  + +  +S SM++  +   +Y+  +F  E    +T DI  +R 
Sbjct: 199 FGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYVTKFFWWEAGYWNTMDIAHDRA 258

Query: 210 GFMLVFGDLVWIPFTFSIQGWWLLRNKVELTTAAIVANCFVFLIGYMVFRGANKQKHEFK 269
           GF + +G LVW+P  ++  G +L+ + VEL T   +      ++   +    ++Q+ EF+
Sbjct: 259 GFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYILVAGILCIYINYDCDRQRQEFR 318

Query: 270 K-NPKAPIWGKPPIVIGGK------------LLASGYWGVARHCNYXXXXXXXXXXXXPC 316
           + N K  +WG+ P  I               LL SG+WG+ARH +Y            P 
Sbjct: 319 RTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLARHFHYVPEILSAFFWTVPA 378

Query: 317 GISSXXXXXXXXXXXXXXXWRERRDEARCAEKYKEIWAEYRRTVPWRILPYVY 369
              +                R +RD+ RC  KY + W  Y   V +RI+P +Y
Sbjct: 379 LFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCEKVKYRIIPGIY 431


>AT1G50430.1 | Symbols: DWF5, PA, LE, ST7R, 7RED | Ergosterol
           biosynthesis ERG4/ERG24 family | chr1:18682175-18685555
           REVERSE LENGTH=432
          Length = 432

 Score =  123 bits (309), Expect = 2e-28,   Method: Compositional matrix adjust.
 Identities = 91/353 (25%), Positives = 156/353 (44%), Gaps = 19/353 (5%)

Query: 33  VVPGKLIAGVVLPDKSRLRYRCNGXXXXXXXXXXXGIGAKMGFVSPTAISERGLELLSTT 92
           ++PGK + G + P  +R  Y+ NG                 G  +P  + +   E+ S  
Sbjct: 83  LLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGLWWFGIFNPAIVYDHLGEIFSAL 142

Query: 93  FIFSFLVALILYFXXXXXXXXXXXXXXXXXGNLIHDWWFGIQLNPQV-MGIDLKFFF-VR 150
              SF+  ++LY                  GNLI D+++G++L P++    D+K F   R
Sbjct: 143 IFGSFIFCVLLYIKGHVAPSSSDSGSC---GNLIIDFYWGMELYPRIGKSFDIKVFTNCR 199

Query: 151 AGMMGWLLINLSVLAKSIQ-DDTLSQSMILFQLFCGIYILDYFVHEEYMTSTWDIIAERL 209
            GMM W ++ ++   K  + +  +S SM++  +   +Y+  +F  E    +T DI  +R 
Sbjct: 200 FGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYVTKFFWWEAGYWNTMDIAHDRA 259

Query: 210 GFMLVFGDLVWIPFTFSIQGWWLLRNKVELTTAAIVANCFVFLIGYMVFRGANKQKHEFK 269
           GF + +G LVW+P  ++  G +L+ + VEL T   +      ++   +    ++Q+ EF+
Sbjct: 260 GFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYILVAGILCIYINYDCDRQRQEFR 319

Query: 270 K-NPKAPIWGKPPIVIGGK------------LLASGYWGVARHCNYXXXXXXXXXXXXPC 316
           + N K  +WG+ P  I               LL SG+WG+ARH +Y            P 
Sbjct: 320 RTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLARHFHYVPEILSAFFWTVPA 379

Query: 317 GISSXXXXXXXXXXXXXXXWRERRDEARCAEKYKEIWAEYRRTVPWRILPYVY 369
              +                R +RD+ RC  KY + W  Y   V +RI+P +Y
Sbjct: 380 LFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCEKVKYRIIPGIY 432