Miyakogusa Predicted Gene

Lj5g3v0611970.2
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v0611970.2 Non Chatacterized Hit- tr|K4BA61|K4BA61_SOLLC
Uncharacterized protein OS=Solanum lycopersicum
GN=Sol,62.13,0,Prokaryotic type I DNA topoisomerase,DNA topoisomerase,
type IA, core domain; no description,Toprim ,CUFF.53333.2
         (1048 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G31210.1 | Symbols:  | DNA topoisomerase, type IA, core | chr...  1170   0.0  
AT5G63920.1 | Symbols: TOP3A, AtTOP3alpha | topoisomerase 3alpha...   111   2e-24
AT2G32000.1 | Symbols:  | DNA topoisomerase, type IA, core | chr...    85   2e-16
AT2G32000.2 | Symbols:  | DNA topoisomerase, type IA, core | chr...    85   3e-16

>AT4G31210.1 | Symbols:  | DNA topoisomerase, type IA, core |
            chr4:15165280-15172534 FORWARD LENGTH=1284
          Length = 1284

 Score = 1170 bits (3028), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 546/818 (66%), Positives = 652/818 (79%), Gaps = 5/818 (0%)

Query: 229  TKPLYPPLGXXXXXXXXXXXXXXIQKYLGDTYEVLPSYGHVRDLAARSGSVRPDDDFSMV 288
            +KPLYPP G              IQ YLGD YEVLPSYGH+RDLA RSGSVRPDDDFSMV
Sbjct: 465  SKPLYPPSGKSVIVVESMTKAKIIQGYLGDMYEVLPSYGHIRDLATRSGSVRPDDDFSMV 524

Query: 289  WEVPSAGWTHLKSFKVALSGAEVFILASDPDREGEAIAWHILEMLQQQGALHDNISVARV 348
            WEVPS+ WTH+KS KVAL+GAE  ILASDPDREGEAIAWHI+EMLQQQGALH++++VARV
Sbjct: 525  WEVPSSAWTHIKSIKVALNGAENLILASDPDREGEAIAWHIIEMLQQQGALHESMTVARV 584

Query: 349  VFHEITEQSIKGALQAPREIDVNLVHAYLARRALDYLIGFNISPLLWKKLPGCQSAGRVQ 408
            VFHEITE +IK ALQ+PREID +LVHAYLARRALDYLIGFNISPLLW+KLPGC SAGRVQ
Sbjct: 585  VFHEITESAIKSALQSPREIDGDLVHAYLARRALDYLIGFNISPLLWRKLPGCPSAGRVQ 644

Query: 409  SAALSLICDREMEIDKFKPQEYWTVEAQLRKKELISNRNLTFSGHLTCFDSKKLTQFSIT 468
            SAAL+L+CDRE EID FKPQEYWTV  +++ K    + + TFS HLT  +SK+L Q SI+
Sbjct: 645  SAALALVCDRESEIDGFKPQEYWTVGIKVKGK----DNSATFSAHLTSLNSKRLNQLSIS 700

Query: 469  SDTEARDIESKLKSTDFKVISLKRNKVRRNPPTPYITSTLQQDAANKLHFTASHTMKLAQ 528
            S+  A+DIE ++KS  F V   K +  R+NPPTPYITSTLQQDAANKLHF+ +HTMKLAQ
Sbjct: 701  SEANAQDIEQRIKSEGFLVKGTKTSTTRKNPPTPYITSTLQQDAANKLHFSTAHTMKLAQ 760

Query: 529  KLYEGVELADGVAEGLITYIRTDGFHISDGAVANIRSLIIERYGQNFAARSPPKYFKKVK 588
            KLYEGV+L+DG + GLITY+RTDG HI+D A+ +I+SL+ ERYG+NF + SP KYFKKVK
Sbjct: 761  KLYEGVQLSDGKSAGLITYMRTDGLHIADEAIKDIQSLVAERYGKNFTSDSPRKYFKKVK 820

Query: 589  NAQEAHEAIRPTDIHKLPSMLAGVLDEESLKLYTLIWSRTVSCQMEPAIFEQVQLDIGNA 648
            NAQEAHEAIRPTDI +LPS +A +LD +SLKLYTLIWSR V+CQMEPA   Q+QLDIGNA
Sbjct: 821  NAQEAHEAIRPTDIRRLPSTIASLLDADSLKLYTLIWSRAVACQMEPASIAQIQLDIGNA 880

Query: 649  DMSIMLRSASSKVEFPGYRAAFEDIDMEVVQDKHNDRSY-RDQAFEVLNSIKTGDLLHLV 707
              SI+ RS+ SKVEF GY+A +ED + + +++  ND+S  R++ F+ L+S+K GDLL + 
Sbjct: 881  SESIIFRSSCSKVEFLGYQAVYEDPEAKAIKNNDNDQSSEREETFKTLSSLKDGDLLQIG 940

Query: 708  QAELNQHHTQPPPRYSEASLVKKLEELGIGRPSTYASTIKVLKDRNYVTVKSRVLSPEFR 767
            + EL QHHTQ PPRYSE SLVKKLEELGIGRPSTYAS  +VL+ R YVT+K+RVL PEFR
Sbjct: 941  EVELKQHHTQHPPRYSEGSLVKKLEELGIGRPSTYASIFRVLQHRKYVTIKNRVLYPEFR 1000

Query: 768  GRMVSAFLSHHFSEVMDYNFTADMETELDNVSGGLTEWKGLLRDYWTRFKSYCDSTSNVH 827
             RMVSAFL+H+F+E+ DY+FTADMETELDNVSGG+TEWKGLLRDYWTRF +YC    NV 
Sbjct: 1001 ARMVSAFLTHYFTEITDYSFTADMETELDNVSGGVTEWKGLLRDYWTRFSAYCKRVENVQ 1060

Query: 828  IHQVEKMLEKKFGQYLFASLRDQKRVCPSCMEGTLIFKVSRFGAGYFIGCDQHPRCKYIA 887
              QVEKMLEKK+   LF+ L    R CPSCMEGTL FK S+FGAGYFIGCDQHP CK+IA
Sbjct: 1061 RSQVEKMLEKKYEDVLFSLLPYPSRTCPSCMEGTLSFKASKFGAGYFIGCDQHPSCKFIA 1120

Query: 888  KTLYGVEEDEDAPQPNTTIEEPKVLGVISGSNEKVLFKSGPYGCYVQLGEDRKGYAPKRA 947
            KTLYG +EDED P  N  +EEPK+LG+   ++EKV+ K GPYG YVQLGED+KG+ PKRA
Sbjct: 1121 KTLYGEDEDEDDPPKNNCVEEPKLLGLHPNTSEKVILKCGPYGHYVQLGEDKKGHTPKRA 1180

Query: 948  SVPSFMNANNITLEDGLELLRYPLTLGKHPKDGRPVILRLSQGGFTVKHRSKSFHVPKDM 1007
            +     + N+ITLE  LELLRYPLTLG HP+DG+PV+L+LS+ GFT+KHR     VPK+ 
Sbjct: 1181 NAAHIKDVNSITLESALELLRYPLTLGTHPEDGQPVVLKLSKSGFTIKHRRNMATVPKNT 1240

Query: 1008 NPRDVTFEKALKFLLGDNVTNTGRPKSKPKVEEDEAVE 1045
             P +VT E+A+K L G NV  +GRP      EE++  E
Sbjct: 1241 EPGEVTLERAMKLLSGKNVRKSGRPPKSILPEEEKGDE 1278


>AT5G63920.1 | Symbols: TOP3A, AtTOP3alpha | topoisomerase 3alpha |
           chr5:25574533-25581230 FORWARD LENGTH=926
          Length = 926

 Score =  111 bits (278), Expect = 2e-24,   Method: Compositional matrix adjust.
 Identities = 138/611 (22%), Positives = 242/611 (39%), Gaps = 89/611 (14%)

Query: 309 AEVFILASDPDREGEAIAWHILEMLQQQGALHDNISVARVVFHEITEQSIKGALQAPREI 368
           ++  +L  D DREGE IA+ ++++ +   A+  N+ + R  F  + ++ I  A+Q  R+ 
Sbjct: 115 SDWLVLWLDCDREGENIAFEVVDVCR---AVKHNLFIRRAHFSALIDRDIHEAVQNLRDP 171

Query: 369 DVNLVHAYLARRALDYLIGFNIS---PLLWKKLPGCQSAGR----------VQSAALSLI 415
           +     A  AR+ +D  IG + +    +L +      S G            Q   L  I
Sbjct: 172 NQLFAEAVDARQEIDLRIGASFTRFQTMLLRDRFAIDSTGEERSRVISYGPCQFPTLGFI 231

Query: 416 CDREMEIDKFKPQEYWTVEAQLRKKELISNRNLTFSGHLTCFDSKKLTQFSITSDTEARD 475
            +R  EI   +P+E+WT+    + +E ++  N    GHL  + S  +       +  A  
Sbjct: 232 VERYWEIQAHEPEEFWTINCSHQSEEGLATFNW-MRGHLFDYASAVILYEMCVEEPTATV 290

Query: 476 IESKLKSTDFKVISLKRNKVRRNPPTPYITSTLQQDAANKLHFTASHTMKLAQKLYEGVE 535
           +        FK            PP P  T  L++ A+     ++ HTMK+A++LY+   
Sbjct: 291 MNVPHPRERFKY-----------PPYPLNTIELEKRASRYFRLSSEHTMKVAEELYQA-- 337

Query: 536 LADGVAEGLITYIRTDGFHISDGAVANIRSLIIER--------YGQNFAARSPPKYFKKV 587
                  G I+Y RT+    S  +  ++R+++ E+        Y Q         +    
Sbjct: 338 -------GFISYPRTETDSFS--SRTDLRAMVEEQTRHPAWGSYAQRLLEPEGGLWRNPA 388

Query: 588 KNAQE--AHEAIRPTDIHKLPSMLAGVLDEESLKLYTLIWSRTVSCQMEPAIFEQ--VQL 643
               +  AH  I PT      S  +     + L +Y L+    ++C  +PA+  +  V++
Sbjct: 389 NGGHDDKAHPPIHPTKFSSGESNWS----RDHLNVYELVVRHYLACVSQPAVAAETTVEI 444

Query: 644 DIGNADMSIMLRSASSKVEFPGYRAAFEDIDMEVVQDKHNDRSYRDQAFEVLNSIKTGDL 703
           DI     S   R+  +K     YR                   +      V+   + G  
Sbjct: 445 DIAGERFSASGRAILAKNYLEVYR-------------------FESWGGSVIPVYEKGQQ 485

Query: 704 LHLVQAELNQHHTQPPPRYSEASLVKKLEELGIGRPSTYASTIKVLKDRNYVTVKSRV-L 762
                  L+   T+PPP   EA L+  +++ GIG  +T    IK L DR Y T  +    
Sbjct: 486 FIPTTLTLDAAVTRPPPLLCEADLLSCMDKAGIGTDATMHDHIKKLLDRGYATKDANTRF 545

Query: 763 SPEFRGRMVSAFLSHHFSEVMDYNFTADMETELDNVSGGLTEWKGLLRDYWTRFKSYCDS 822
           SP   G  +         E+   N  A ME +++ VS G      +L     + K+ C  
Sbjct: 546 SPTNLGEALVMGYDDMGYELWKPNLRALMEHDMNEVSVGRKTKAEVLETCLQQMKA-CFL 604

Query: 823 TSNVHIHQVEKMLEKKFGQYLFASLRDQK---------RVCPSCMEGTLIFKVSRFGAGY 873
            + V   +  K+LE     +  ++  D+          R C  C E  +  + +R G  +
Sbjct: 605 DARV---KKSKLLEAMTIFFERSNNTDESESQTAGEVVRRCNLCNESDMALRKNRDG-NF 660

Query: 874 FIGCDQHPRCK 884
            +GC  +P+C+
Sbjct: 661 MVGCMNYPQCR 671


>AT2G32000.1 | Symbols:  | DNA topoisomerase, type IA, core |
           chr2:13615999-13621563 REVERSE LENGTH=865
          Length = 865

 Score = 85.1 bits (209), Expect = 2e-16,   Method: Compositional matrix adjust.
 Identities = 112/472 (23%), Positives = 192/472 (40%), Gaps = 73/472 (15%)

Query: 308 GAEVFILASDPDREGEAIAWHILEMLQQQGALHDNI-SVARVVFHEITEQSIKGALQAPR 366
           G    +L  D DREGE I + ++E       + D+   V R  F  +TE+ I  A+    
Sbjct: 107 GCSYMVLWLDCDREGENICFEVIE--STGFDMKDSKRKVYRARFSSVTEKDISKAMDNLV 164

Query: 367 EIDVNLVHAYLARRALDYLIGFNISPLLWKKLPG------CQ--SAGRVQSAALSLICDR 418
           E + +   A  AR+ +D  +G   S        G      C+  S G  Q+  L     R
Sbjct: 165 EPNRDEALAVDARQEIDLKVGVAFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQR 224

Query: 419 EMEIDKFKPQEYWTVEAQLRKKELISNRNLTFSGHLTCFDSKKLTQFSITSDTEARDIES 478
            M I+ FKP+++W +   +RK            G+    + ++   F    D EA  +  
Sbjct: 225 YMHINTFKPEKFWALRPYIRK-----------DGYELQLEWERRRLF----DLEAATVFQ 269

Query: 479 KL--KSTDFKVISLKRNKVRRNPPTPYITSTLQQDAANKLHFTASHTMKLAQKLYEGVEL 536
           KL  +    KV+ +   +  +  P    T  L + A++ L F     M LA++LY     
Sbjct: 270 KLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVASSALGFGPQTAMHLAERLY----- 324

Query: 537 ADGVAEGLITYIRTD------GFHISDGAVANIRSLIIERYGQNFAARSPPKYFKKVKNA 590
                +G I+Y RT+       F  +D   A + + +   Y Q   +      F   K+ 
Sbjct: 325 ----TQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLLSDG----FHMPKSG 376

Query: 591 QEAHEAIRPTDIHKLPSMLAGVLDEESLKLYTLIWSR---TVSCQMEPAIFEQVQLDIGN 647
            +A +    T +     ++ G    ++ +LY  +      TVS   +  I  +V+L IG 
Sbjct: 377 TDAGDHPPITPMRAATEVMVG---GDAWRLYQYVCQHFLGTVSPNCK-YIRTKVELSIGG 432

Query: 648 ADMSIMLRSASSKVEFPGYRAAFEDIDMEVVQDKHNDRSYRDQAFEVLNSIKTGDLLHLV 707
                  +  + K    G+ A    +    V +K             L S   G+ + ++
Sbjct: 433 ETFHCTGQRVTEK----GFTAI---MPWSAVDEKK------------LPSFLKGERIEVL 473

Query: 708 QAELNQHHTQPPPRYSEASLVKKLEELGIGRPSTYASTIKVLKDRNYVTVKS 759
           + EL + +T PP   +E+ L+  +E+ GIG  ++ A  I  + +RNYV V+S
Sbjct: 474 RVELYEGNTAPPDYLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQS 525


>AT2G32000.2 | Symbols:  | DNA topoisomerase, type IA, core |
           chr2:13615999-13621397 REVERSE LENGTH=838
          Length = 838

 Score = 85.1 bits (209), Expect = 3e-16,   Method: Compositional matrix adjust.
 Identities = 113/474 (23%), Positives = 193/474 (40%), Gaps = 77/474 (16%)

Query: 308 GAEVFILASDPDREGEAIAWHILEMLQQQGALHDNI-SVARVVFHEITEQSIKGALQAPR 366
           G    +L  D DREGE I + ++E       + D+   V R  F  +TE+ I  A+    
Sbjct: 80  GCSYMVLWLDCDREGENICFEVIE--STGFDMKDSKRKVYRARFSSVTEKDISKAMDNLV 137

Query: 367 EIDVNLVHAYLARRALDYLIGFNISPLLWKKLPG------CQ--SAGRVQSAALSLICDR 418
           E + +   A  AR+ +D  +G   S        G      C+  S G  Q+  L     R
Sbjct: 138 EPNRDEALAVDARQEIDLKVGVAFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQR 197

Query: 419 EMEIDKFKPQEYWTVEAQLRKKELISNRNLTFSGHLTCFDSKKLTQFSITSDTEARDIES 478
            M I+ FKP+++W +   +RK            G+    + ++   F    D EA  +  
Sbjct: 198 YMHINTFKPEKFWALRPYIRK-----------DGYELQLEWERRRLF----DLEAATVFQ 242

Query: 479 KL--KSTDFKVISLKRNKVRRNPPTPYITSTLQQDAANKLHFTASHTMKLAQKLYEGVEL 536
           KL  +    KV+ +   +  +  P    T  L + A++ L F     M LA++LY     
Sbjct: 243 KLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVASSALGFGPQTAMHLAERLY----- 297

Query: 537 ADGVAEGLITYIRTD------GFHISDGAVANIRSLIIERYGQNFAARSPPKYFKKVKNA 590
                +G I+Y RT+       F  +D   A + + +   Y Q   +      F   K+ 
Sbjct: 298 ----TQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLLSDG----FHMPKSG 349

Query: 591 QEAHEAIRPTDIHKLPSMLAGVLDEESLKLYTLIWSR---TVS--CQMEPAIFEQVQLDI 645
            +A +    T +     ++ G    ++ +LY  +      TVS  C+    I  +V+L I
Sbjct: 350 TDAGDHPPITPMRAATEVMVG---GDAWRLYQYVCQHFLGTVSPNCKY---IRTKVELSI 403

Query: 646 GNADMSIMLRSASSKVEFPGYRAAFEDIDMEVVQDKHNDRSYRDQAFEVLNSIKTGDLLH 705
           G        +  + K    G+ A    +    V +K             L S   G+ + 
Sbjct: 404 GGETFHCTGQRVTEK----GFTAI---MPWSAVDEKK------------LPSFLKGERIE 444

Query: 706 LVQAELNQHHTQPPPRYSEASLVKKLEELGIGRPSTYASTIKVLKDRNYVTVKS 759
           +++ EL + +T PP   +E+ L+  +E+ GIG  ++ A  I  + +RNYV V+S
Sbjct: 445 VLRVELYEGNTAPPDYLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQS 498