Miyakogusa Predicted Gene

Lj5g3v0279140.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v0279140.1 Non Chatacterized Hit- tr|I3S981|I3S981_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2 SV=1,98.96,0,seg,NULL;
no description,NAD(P)-binding domain; 3Beta_HSD,3-beta hydroxysteroid
dehydrogenase/isomer,CUFF.52703.1
         (479 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G33630.1 | Symbols:  | NAD(P)-binding Rossmann-fold superfami...   669   0.0  
AT1G47290.2 | Symbols: AT3BETAHSD/D1, 3BETAHSD/D1 | 3beta-hydrox...   185   5e-47
AT1G47290.1 | Symbols: AT3BETAHSD/D1, 3BETAHSD/D1 | 3beta-hydrox...   185   6e-47
AT2G26260.1 | Symbols: AT3BETAHSD/D2, 3BETAHSD/D2 | 3beta-hydrox...   184   1e-46
AT2G26260.2 | Symbols: AT3BETAHSD/D2, 3BETAHSD/D2 | 3beta-hydrox...   166   3e-41
AT2G43420.1 | Symbols:  | 3-beta hydroxysteroid dehydrogenase/is...   107   3e-23

>AT2G33630.1 | Symbols:  | NAD(P)-binding Rossmann-fold superfamily
           protein | chr2:14236951-14238734 REVERSE LENGTH=480
          Length = 480

 Score =  669 bits (1725), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 317/479 (66%), Positives = 386/479 (80%), Gaps = 2/479 (0%)

Query: 1   MHLSENEGIEXXXXXXXXXXXXXXSALCLELVRRGAHEVRAFDLRDSSPWFAILKDNGVR 60
           MHLSENEG+E              +ALCLELVRRGA +VR+FDLR SSPW   LK++GVR
Sbjct: 1   MHLSENEGVEGNTFVVTGGLGFVGAALCLELVRRGARQVRSFDLRHSSPWSDDLKNSGVR 60

Query: 61  CIQGDIVRKEDVESALRGADCVFHLAAFGMSGKEMLQLTRVDQVNITGTCHVLDACLHLG 120
           CIQGD+ +K+DV++AL GADCV HLA++GMSGKEML+  R D+VNI GTC+VL+A     
Sbjct: 61  CIQGDVTKKQDVDNALDGADCVLHLASYGMSGKEMLRFGRCDEVNINGTCNVLEAAFKHE 120

Query: 121 IKRLVYCSTYNVVFAGQRIVNGNESLPYFPIDHHVDPYGRSKSIAEQLVLKNNARPFKND 180
           I R+VY STYNVVF G+ I+NGNE LPYFP+D HVD Y R+KSIAEQLVLK+N RPFKN 
Sbjct: 121 ITRIVYVSTYNVVFGGKEILNGNEGLPYFPLDDHVDAYSRTKSIAEQLVLKSNGRPFKN- 179

Query: 181 AGNCLYTCAVRPAAIYGPGEDRHLPRIITTAKLGLLLFTIGDQTVKSDWVFVENLVLALI 240
            G  +YTCA+RPAAIYGPGEDRHLPRI+T  KLGL LF IG+ +VKSDW++VENLVLA+I
Sbjct: 180 GGKRMYTCAIRPAAIYGPGEDRHLPRIVTLTKLGLALFKIGEPSVKSDWIYVENLVLAII 239

Query: 241 LASMGLLDDSAGKGKQRPIAAGQAYFISDGSPVNTFEFLQPLLRSLEYELPKTSLAVDHA 300
           LASMGLLDD  G+  Q P+AAGQ YF+SDG PVNTFEFL+PLL+SL+Y+LPK +++V  A
Sbjct: 240 LASMGLLDDIPGREGQ-PVAAGQPYFVSDGYPVNTFEFLRPLLKSLDYDLPKCTISVPFA 298

Query: 301 LVLGRICQGVYTILYPWLDRWWLPQPFILPSEVHKVGVTHYFSYLKAKEEIGYVPMVSSR 360
           L LG+I QG YT+LYPWL + WLPQP +LP+EV+KVGVTHYFSYLKAKEE+GYVP  SS+
Sbjct: 299 LSLGKIFQGFYTVLYPWLSKSWLPQPLVLPAEVYKVGVTHYFSYLKAKEELGYVPFKSSK 358

Query: 361 EGMASTISYWQQRKMITLDGPTIYTWLFCVIGMISLFCAAFLPDVGIVFLLRATSLFVFR 420
           EGMA+TISYWQ+RK  +LDGPT++TW+   IGM +LF A +LPD+G V  LRA  LF FR
Sbjct: 359 EGMAATISYWQERKRRSLDGPTMFTWIAVTIGMSALFAAGWLPDIGPVPFLRAIHLFFFR 418

Query: 421 SMWMIRLVFILATAAHVFEAIYAWYLAKRVDHANARGWFWQTFALGYFSLRFLLKRARK 479
           ++ +++ VFI+A   HV E IYAW+LAKRVD  NA GWF QT ALG+FS+RFLLKRA++
Sbjct: 419 TITIVKAVFIVAVVLHVAEGIYAWFLAKRVDPGNAMGWFLQTSALGFFSMRFLLKRAKE 477


>AT1G47290.2 | Symbols: AT3BETAHSD/D1, 3BETAHSD/D1 |
           3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
           1 | chr1:17336121-17339030 FORWARD LENGTH=439
          Length = 439

 Score =  185 bits (470), Expect = 5e-47,   Method: Compositional matrix adjust.
 Identities = 113/323 (34%), Positives = 173/323 (53%), Gaps = 23/323 (7%)

Query: 54  LKDNGVRCIQGDIVRKEDVESALRGADCVFHLAAFGMS-GKEMLQLTRVDQVNITGTCHV 112
           ++   V+ +  D+  K  V    +GA+ VFH+AA   S     LQ +    VN+ GT +V
Sbjct: 61  IRSGRVQYVSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYS----VNVQGTTNV 116

Query: 113 LDACLHLGIKRLVYCSTYNVVFAG-QRIVNGNESLPYFPIDHHVDPYGRSKSIAEQLVLK 171
           +DAC+ +G+KRL+Y S+ +VVF G    +N +ESLPY P   H D Y  +K+  E L+LK
Sbjct: 117 IDACIEVGVKRLIYTSSPSVVFDGVHGTLNADESLPYPP--KHNDSYSATKAEGEALILK 174

Query: 172 NNARPFKNDAGNCLYTCAVRPAAIYGPGEDRHLPRIITTAKLGLLLFTIGDQTVKSDWVF 231
            N R       + L TC +RP++I+GPG+   +P ++T A+ G   F IGD +   D+ +
Sbjct: 175 ANGR-------SGLLTCCIRPSSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTY 227

Query: 232 VENLVLALILASMGLLDDSAGKGKQRPIAAGQAYFISDGSPVNTFEFLQPLLRSLEYELP 291
           VEN+V A + A   L    A  G+    AAGQAYFI++  P+  +EF+  LL  L YE P
Sbjct: 228 VENVVHAHVCAERAL----ASGGEVCAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERP 283

Query: 292 KTSLAVDHALVLGRICQGVYTILYPWLDRWWLPQPFILPSEVHKVGVTHYFSYLKAKEEI 351
              +     + +  + +  Y +L P    + +  P + PS V  +     F   KAK+ +
Sbjct: 284 SIKIPASLMMPIAYLVELAYKLLGP----YGMKVPVLTPSRVRLLSCNRTFDSSKAKDRL 339

Query: 352 GYVPMVSSREGMASTISYWQQRK 374
           GY P+V  +EG+  TI  +   K
Sbjct: 340 GYSPVVPLQEGIKRTIDSFSHLK 362


>AT1G47290.1 | Symbols: AT3BETAHSD/D1, 3BETAHSD/D1 |
           3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
           1 | chr1:17336121-17339030 FORWARD LENGTH=382
          Length = 382

 Score =  185 bits (470), Expect = 6e-47,   Method: Compositional matrix adjust.
 Identities = 113/323 (34%), Positives = 173/323 (53%), Gaps = 23/323 (7%)

Query: 54  LKDNGVRCIQGDIVRKEDVESALRGADCVFHLAAFGMS-GKEMLQLTRVDQVNITGTCHV 112
           ++   V+ +  D+  K  V    +GA+ VFH+AA   S     LQ +    VN+ GT +V
Sbjct: 61  IRSGRVQYVSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYS----VNVQGTTNV 116

Query: 113 LDACLHLGIKRLVYCSTYNVVFAG-QRIVNGNESLPYFPIDHHVDPYGRSKSIAEQLVLK 171
           +DAC+ +G+KRL+Y S+ +VVF G    +N +ESLPY P   H D Y  +K+  E L+LK
Sbjct: 117 IDACIEVGVKRLIYTSSPSVVFDGVHGTLNADESLPYPP--KHNDSYSATKAEGEALILK 174

Query: 172 NNARPFKNDAGNCLYTCAVRPAAIYGPGEDRHLPRIITTAKLGLLLFTIGDQTVKSDWVF 231
            N R       + L TC +RP++I+GPG+   +P ++T A+ G   F IGD +   D+ +
Sbjct: 175 ANGR-------SGLLTCCIRPSSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTY 227

Query: 232 VENLVLALILASMGLLDDSAGKGKQRPIAAGQAYFISDGSPVNTFEFLQPLLRSLEYELP 291
           VEN+V A + A   L    A  G+    AAGQAYFI++  P+  +EF+  LL  L YE P
Sbjct: 228 VENVVHAHVCAERAL----ASGGEVCAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERP 283

Query: 292 KTSLAVDHALVLGRICQGVYTILYPWLDRWWLPQPFILPSEVHKVGVTHYFSYLKAKEEI 351
              +     + +  + +  Y +L P    + +  P + PS V  +     F   KAK+ +
Sbjct: 284 SIKIPASLMMPIAYLVELAYKLLGP----YGMKVPVLTPSRVRLLSCNRTFDSSKAKDRL 339

Query: 352 GYVPMVSSREGMASTISYWQQRK 374
           GY P+V  +EG+  TI  +   K
Sbjct: 340 GYSPVVPLQEGIKRTIDSFSHLK 362


>AT2G26260.1 | Symbols: AT3BETAHSD/D2, 3BETAHSD/D2 |
           3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
           2 | chr2:11178237-11182872 FORWARD LENGTH=564
          Length = 564

 Score =  184 bits (467), Expect = 1e-46,   Method: Compositional matrix adjust.
 Identities = 114/321 (35%), Positives = 173/321 (53%), Gaps = 23/321 (7%)

Query: 54  LKDNGVRCIQGDIVRKEDVESALRGADCVFHLAAFGMS-GKEMLQLTRVDQVNITGTCHV 112
           L+   V+ I  D+  K  V  A +GA+ VFH+AA   S     LQ +    VN+ GT +V
Sbjct: 61  LRSGRVQYISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYS----VNVQGTQNV 116

Query: 113 LDACLHLGIKRLVYCSTYNVVFAG-QRIVNGNESLPYFPIDHHVDPYGRSKSIAEQLVLK 171
           +DAC+ +G+KRL+Y S+ +VVF G   I+NG ES+ Y PI H+ D Y  +K+  E+L++K
Sbjct: 117 IDACVDVGVKRLIYTSSPSVVFDGVHGILNGTESMAY-PIKHN-DSYSATKAEGEELIMK 174

Query: 172 NNARPFKNDAGNCLYTCAVRPAAIYGPGEDRHLPRIITTAKLGLLLFTIGDQTVKSDWVF 231
            N R       N L TC +RP++I+GPG+   +P ++  A+ G   F IGD     D+ +
Sbjct: 175 ANGR-------NGLLTCCIRPSSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTY 227

Query: 232 VENLVLALILASMGLLDDSAGKGKQRPIAAGQAYFISDGSPVNTFEFLQPLLRSLEYELP 291
           VEN+  A + A   L    A  G     AAGQAYFI++  P+  +EF+  LL  L YE P
Sbjct: 228 VENVAHAHVCAERAL----ASGGDVSTKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERP 283

Query: 292 KTSLAVDHALVLGRICQGVYTILYPWLDRWWLPQPFILPSEVHKVGVTHYFSYLKAKEEI 351
              +     + +  + +  Y +L P    + +  P + PS V  +  +  F   KAK+ +
Sbjct: 284 SIKIPAFIMMPIAHLVELTYKVLGP----YGMTVPQLTPSRVRLLSCSRTFDSTKAKDRL 339

Query: 352 GYVPMVSSREGMASTISYWQQ 372
           GY P+V  +EG+  TI  +  
Sbjct: 340 GYAPVVPLQEGIRRTIDSFSH 360


>AT2G26260.2 | Symbols: AT3BETAHSD/D2, 3BETAHSD/D2 |
           3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
           2 | chr2:11178586-11182872 FORWARD LENGTH=473
          Length = 473

 Score =  166 bits (420), Expect = 3e-41,   Method: Compositional matrix adjust.
 Identities = 97/271 (35%), Positives = 149/271 (54%), Gaps = 18/271 (6%)

Query: 103 QVNITGTCHVLDACLHLGIKRLVYCSTYNVVFAG-QRIVNGNESLPYFPIDHHVDPYGRS 161
            VN+ GT +V+DAC+ +G+KRL+Y S+ +VVF G   I+NG ES+ Y PI H+ D Y  +
Sbjct: 16  SVNVQGTQNVIDACVDVGVKRLIYTSSPSVVFDGVHGILNGTESMAY-PIKHN-DSYSAT 73

Query: 162 KSIAEQLVLKNNARPFKNDAGNCLYTCAVRPAAIYGPGEDRHLPRIITTAKLGLLLFTIG 221
           K+  E+L++K N R       N L TC +RP++I+GPG+   +P ++  A+ G   F IG
Sbjct: 74  KAEGEELIMKANGR-------NGLLTCCIRPSSIFGPGDRLLVPSLVAAARAGKSKFIIG 126

Query: 222 DQTVKSDWVFVENLVLALILASMGLLDDSAGKGKQRPIAAGQAYFISDGSPVNTFEFLQP 281
           D     D+ +VEN+  A + A   L    A  G     AAGQAYFI++  P+  +EF+  
Sbjct: 127 DGNNLYDFTYVENVAHAHVCAERAL----ASGGDVSTKAAGQAYFITNMEPIKFWEFMSQ 182

Query: 282 LLRSLEYELPKTSLAVDHALVLGRICQGVYTILYPWLDRWWLPQPFILPSEVHKVGVTHY 341
           LL  L YE P   +     + +  + +  Y +L P    + +  P + PS V  +  +  
Sbjct: 183 LLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGP----YGMTVPQLTPSRVRLLSCSRT 238

Query: 342 FSYLKAKEEIGYVPMVSSREGMASTISYWQQ 372
           F   KAK+ +GY P+V  +EG+  TI  +  
Sbjct: 239 FDSTKAKDRLGYAPVVPLQEGIRRTIDSFSH 269


>AT2G43420.1 | Symbols:  | 3-beta hydroxysteroid
           dehydrogenase/isomerase family protein |
           chr2:18031493-18034936 REVERSE LENGTH=561
          Length = 561

 Score =  107 bits (266), Expect = 3e-23,   Method: Compositional matrix adjust.
 Identities = 92/324 (28%), Positives = 153/324 (47%), Gaps = 35/324 (10%)

Query: 65  DIVRKEDVESALRGADCVFHLAAFGMSGKEMLQLTRVDQVNITGTCHVLDACLHLGIKRL 124
           D+  K  +     G+  VF++ A  +   +     +V    + GT +V+ AC   G+++L
Sbjct: 73  DVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKV---IVQGTRNVISACRESGVRKL 129

Query: 125 VYCSTYNVVFAG-QRIVNGNESLPYFPIDHHVDPYGRSKSIAEQLVLKNNARPFKNDAGN 183
           +Y ST +VVF G Q I +G+ESL   P+          K+ AE L+   N R       +
Sbjct: 130 IYNSTADVVFDGSQPIRDGDESLRR-PLKFQ-SMLTDFKAQAEALIKLANNR-------D 180

Query: 184 CLYTCAVRPAAIYGPGEDRHLPRIITTAKLGLLLFTIGDQTVKSDWVFVENLVLALILAS 243
            L TCA+R + ++GPG+   +P ++  AK G   F +G     SD+ + EN+  A I A 
Sbjct: 181 GLLTCALRSSIVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHICAV 240

Query: 244 MGLLDDSAGKGKQRPIAAGQAYFISDGSPVNTFEFLQPLLRSLEYELPKTSLAVDHALVL 303
             L  DS     Q    AG+ +FI++  PV  ++F+  ++  L Y  P   L V      
Sbjct: 241 KAL--DS-----QMEFVAGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPV------ 287

Query: 304 GRICQGVYTILYPWLDRWWLPQPFILPSEVHKVGV----THYFSYLKAKEEIGYVPMVSS 359
            R+   V+++L    ++  L   +      H+  +    T  F+   AK+ +GY P+V+ 
Sbjct: 288 -RLVLYVFSLLKWTHEKEGLGSNY---DTAHQYALLASSTRTFNCNAAKKHLGYTPVVTL 343

Query: 360 REGMASTISYWQQRKMITLDGPTI 383
            +G+AST+  W  R +   D   I
Sbjct: 344 EDGIASTLQ-WFSRDLEKSDDTII 366