Miyakogusa Predicted Gene

Lj4g3v2846320.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v2846320.1 Non Chatacterized Hit- tr|G7LFS3|G7LFS3_MEDTR
Putative uncharacterized protein OS=Medicago
truncatul,76.83,0,membrane-attack complex / perforin,Membrane attack
complex component/perforin (MACPF) domain;
MACPF,,NODE_48038_length_1110_cov_162.866669.path2.1
         (319 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G24290.1 | Symbols:  | MAC/Perforin domain-containing protein...   362   e-100
AT4G24290.2 | Symbols:  | MAC/Perforin domain-containing protein...   361   e-100
AT1G28380.1 | Symbols: NSL1 | MAC/Perforin domain-containing pro...   282   2e-76
AT1G14780.1 | Symbols:  | MAC/Perforin domain-containing protein...   237   1e-62
AT1G29690.1 | Symbols: CAD1 | MAC/Perforin domain-containing pro...   221   5e-58

>AT4G24290.1 | Symbols:  | MAC/Perforin domain-containing protein |
           chr4:12594856-12596590 FORWARD LENGTH=350
          Length = 350

 Score =  362 bits (928), Expect = e-100,   Method: Compositional matrix adjust.
 Identities = 184/311 (59%), Positives = 229/311 (73%), Gaps = 9/311 (2%)

Query: 17  AEDGIKCIGLGFDLTNDLKLKFCKADS--NRLITID--DDNN---LPGHVSTPNVPKSIK 69
           AE  I  IG G+DL  DL+LK+CK  S  +RL+ I   DDN    LPG +S PNV KSIK
Sbjct: 11  AEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIK 70

Query: 70  CDKGDRMRLGSDVLSFQQMSEQFNQELSLSGKIPTGHFNAAFDFSGGWQKDAANTKTLAF 129
           CDKG+RMR  SD+L FQQM+EQFNQELSL+GKIP+G FNA F+FS  WQKDAA TK LAF
Sbjct: 71  CDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAF 130

Query: 130 DGVSITLYSIALEKTQVGLRDHVRQAVPSSWDPAALARFIEKYGTHVIVGVKLGGTDIIY 189
           DGV I+LYS+AL+K+QV LR+HV+QAVPS+WDPAALARFI+ YGTH+IV VK+GG D+IY
Sbjct: 131 DGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIY 190

Query: 190 AKQQYSSPVQPVEVRKLLKDMADNNFLGQAGQHNAHDGKSNTKEKF-MMDNGLDFMGIQP 248
           AKQQ+SS +QP +++K LK++AD  F+  +  HN    +     K    +  L F     
Sbjct: 191 AKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSS 250

Query: 249 RSYYEAEVQDIKFMCKRKGGNLKKSMTHNDWCQTVLSQPDLIAMSFIPITSLLGGVNGSG 308
              Y A  +D  FMCKR+GGN  +++ HN+W QTV  +PD+I+MSFIPITSLL GV GSG
Sbjct: 251 LGSY-ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSG 309

Query: 309 YLTHAINLYLR 319
           +L+HAINLYLR
Sbjct: 310 FLSHAINLYLR 320


>AT4G24290.2 | Symbols:  | MAC/Perforin domain-containing protein |
           chr4:12594856-12597815 FORWARD LENGTH=606
          Length = 606

 Score =  361 bits (926), Expect = e-100,   Method: Compositional matrix adjust.
 Identities = 184/311 (59%), Positives = 229/311 (73%), Gaps = 9/311 (2%)

Query: 17  AEDGIKCIGLGFDLTNDLKLKFCKADS--NRLITID--DDNN---LPGHVSTPNVPKSIK 69
           AE  I  IG G+DL  DL+LK+CK  S  +RL+ I   DDN    LPG +S PNV KSIK
Sbjct: 11  AEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIK 70

Query: 70  CDKGDRMRLGSDVLSFQQMSEQFNQELSLSGKIPTGHFNAAFDFSGGWQKDAANTKTLAF 129
           CDKG+RMR  SD+L FQQM+EQFNQELSL+GKIP+G FNA F+FS  WQKDAA TK LAF
Sbjct: 71  CDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAF 130

Query: 130 DGVSITLYSIALEKTQVGLRDHVRQAVPSSWDPAALARFIEKYGTHVIVGVKLGGTDIIY 189
           DGV I+LYS+AL+K+QV LR+HV+QAVPS+WDPAALARFI+ YGTH+IV VK+GG D+IY
Sbjct: 131 DGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIY 190

Query: 190 AKQQYSSPVQPVEVRKLLKDMADNNFLGQAGQHNAHDGKSNTKEKF-MMDNGLDFMGIQP 248
           AKQQ+SS +QP +++K LK++AD  F+  +  HN    +     K    +  L F     
Sbjct: 191 AKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSS 250

Query: 249 RSYYEAEVQDIKFMCKRKGGNLKKSMTHNDWCQTVLSQPDLIAMSFIPITSLLGGVNGSG 308
              Y A  +D  FMCKR+GGN  +++ HN+W QTV  +PD+I+MSFIPITSLL GV GSG
Sbjct: 251 LGSY-ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSG 309

Query: 309 YLTHAINLYLR 319
           +L+HAINLYLR
Sbjct: 310 FLSHAINLYLR 320


>AT1G28380.1 | Symbols: NSL1 | MAC/Perforin domain-containing
           protein | chr1:9963696-9966060 FORWARD LENGTH=612
          Length = 612

 Score =  282 bits (722), Expect = 2e-76,   Method: Compositional matrix adjust.
 Identities = 148/315 (46%), Positives = 202/315 (64%), Gaps = 15/315 (4%)

Query: 17  AEDGIKCIGLGFDLTNDLKLKFCKA--DSNRLITIDDDNN----LPGHVSTPNVPKSIKC 70
           AE  +  IGLG+DL +D++   CK   D +RL+ ID   N     PG +   NV  SIKC
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKC 74

Query: 71  DKGDRMRLGSDVLSFQQMSEQFNQELSLSGKIPTGHFNAAFDFSGGWQKDAANTKTLAFD 130
           DKG+R RL SD+LSF QMSE+FNQ++ LSGKIP+G FN  F FS  W KDA++ KTLA+D
Sbjct: 75  DKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYD 134

Query: 131 GVSITLYSIALEKTQVGLRDHVRQAVPSSWDPAALARFIEKYGTHVIVGVKLGGTDIIYA 190
           G  I+LYS+ + + Q+ LRD V++ VPSSWD AALA FIEKYGTHV+VGV +GG D+I+ 
Sbjct: 135 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 194

Query: 191 KQQYSSPVQPVEVRKLLKDMADNNF----LGQAGQHNAHDGKSNTKEKFMMDNGLDFMGI 246
           KQ   S  +P E++K+LK   D  F    +      + + GK   KE+ ++  GL   G 
Sbjct: 195 KQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKP--KEENLLQWGLQPFGT 252

Query: 247 QPRS--YYEAEVQDIKFMCKRKGGNLKKSMTHNDWCQTVLSQPDLIAMSFIPITSLLGGV 304
              S      + ++I  +C R+GG +    +H  W  TV   P++I+M F+PITSLL G+
Sbjct: 253 SVSSAVVMHTKNEEIMRVCIRRGG-VDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGL 311

Query: 305 NGSGYLTHAINLYLR 319
            G+G+L+HA+NLYLR
Sbjct: 312 PGTGFLSHAVNLYLR 326


>AT1G14780.1 | Symbols:  | MAC/Perforin domain-containing protein |
           chr1:5091020-5093873 FORWARD LENGTH=627
          Length = 627

 Score =  237 bits (604), Expect = 1e-62,   Method: Compositional matrix adjust.
 Identities = 134/322 (41%), Positives = 186/322 (57%), Gaps = 24/322 (7%)

Query: 18  EDGIKCIGLGFDLTNDLKLKFCK-----ADSNRLITIDDDNN----LPGHVSTPNVPKSI 68
           E  +K +G GFDLT D +LK+CK     A  +RL+ +D   N    +PG     NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 69  KCDKGDRMRLGSDVLSFQQMSEQFNQELSLSGKIPTGHFNAAFDF-SGGWQKDAANTKTL 127
            CDKG+R R  SD+L F +MSE FNQ  S++GKIP+G+FNA F F SG W  DAAN K+L
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 128 AFDGVSITLYSIALEK-TQVGLRDHVRQAVPSSWDPAALARFIEKYGTHVIVGVKLGGTD 186
             D   +TL+++ +    ++ L D VR AVPSSWDP  LARFIE+YGTHVI GV +GG D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 187 IIYAKQQYSSPVQPVEVRKLLKDMADNNFLGQA---------GQHNAHDGKSNTKEKFMM 237
           ++  +Q  SS +    +R  L D+ D  F G             H++H      +   + 
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVF 249

Query: 238 DNGLDFMGIQPRSYYEAEVQDIKFMCKRKGGNLKKSMTHNDWCQTVLSQPDLIAMSFIPI 297
           D   D   +   ++       I  +C ++GG+  ++ +H++W  TV  +PD I  +FIPI
Sbjct: 250 D---DKQTVAFNNFSINSQNGITVICAKRGGD-GRAKSHSEWLITVPDKPDAINFNFIPI 305

Query: 298 TSLLGGVNGSGYLTHAINLYLR 319
           TSLL  V GSG L+HA++LYLR
Sbjct: 306 TSLLKDVPGSGLLSHAMSLYLR 327


>AT1G29690.1 | Symbols: CAD1 | MAC/Perforin domain-containing
           protein | chr1:10379310-10381861 REVERSE LENGTH=561
          Length = 561

 Score =  221 bits (563), Expect = 5e-58,   Method: Compositional matrix adjust.
 Identities = 122/307 (39%), Positives = 178/307 (57%), Gaps = 33/307 (10%)

Query: 18  EDGIKCIGLGFDLTNDLKLKFCK-ADSNRLITIDDDNNLPGHVS----TPNVPKSIKCDK 72
            + I+ +G GFD+T+D++L +CK A  +RL+ I++  N    +S     PNVP  I C +
Sbjct: 22  RNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSR 81

Query: 73  GDRMRLGSDVLSFQQMSEQFNQELSLSGKIPTGHFNAAFDFSGGWQKDAANTKTLAFDGV 132
           G+       V SF +M+E+FN    + G IP G FNA F+++G WQ DAA+TK+LA  G 
Sbjct: 82  GNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGY 141

Query: 133 SITLYSIALEKTQVGLRDHVRQAVPSSWDPAALARFIEKYGTHVIVGVKLGGTDIIYAKQ 192
            I LY + L K  + L + +R+AVPSSWDPA+LA FIE YGTH++  V +GG D++Y +Q
Sbjct: 142 FIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQ 201

Query: 193 QYSSPVQPVEVRKLLKDMADNNFLGQAGQHNAHDGKSNTKEKFMMDNGLDFMGIQPRSYY 252
             SSP+   E+   + DM  + F         H+ +S +                P  Y 
Sbjct: 202 HQSSPLPVSEIENYVNDMIKHRF---------HEAESQSITG-------------PLKYK 239

Query: 253 EAEVQDIKFMCKRKGG-NLKKSMTHNDWCQTVLSQPDLIAMSFIPITSLLGGVNGSGYLT 311
           +   +DI  + +R+GG +L++S  H  W +TV + PD+I M+F PI SLL GV G  +LT
Sbjct: 240 D---KDITVIFRRRGGDDLEQS--HARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLT 294

Query: 312 HAINLYL 318
            AI LYL
Sbjct: 295 RAIELYL 301