Miyakogusa Predicted Gene

Lj4g3v2604480.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v2604480.1 Non Chatacterized Hit- tr|I1K3Y1|I1K3Y1_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.22797 PE,87,0,Acetyl-CoA
synthetase-like,NULL; no description,NULL; AMP-binding,AMP-dependent
synthetase/ligase; F,CUFF.51286.1
         (446 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G55320.1 | Symbols: AAE18 | acyl-activating enzyme 18 | chr1:...   691   0.0  
AT1G55320.2 | Symbols: AAE18 | acyl-activating enzyme 18 | chr1:...   520   e-147
AT5G23050.1 | Symbols: AAE17 | acyl-activating enzyme 17 | chr5:...   447   e-125
AT5G36880.1 | Symbols: ACS | acetyl-CoA synthetase | chr5:145349...    80   2e-15
AT5G36880.2 | Symbols: ACS | acetyl-CoA synthetase | chr5:145349...    80   3e-15

>AT1G55320.1 | Symbols: AAE18 | acyl-activating enzyme 18 |
           chr1:20633371-20636659 FORWARD LENGTH=727
          Length = 727

 Score =  691 bits (1784), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 328/446 (73%), Positives = 377/446 (84%), Gaps = 4/446 (0%)

Query: 1   METHASKLLGPSYKDPISSFHLFQKFSVQHPQVYWSLILKELSLSFVEPPRCILDTSDPL 60
           ME H  +LLGPSYK+P+ SF LF++FSV+HP+VYWS ++ ELSL F  PPRCIL+ S P 
Sbjct: 102 MEYHGPRLLGPSYKNPLESFELFRRFSVEHPEVYWSFVIDELSLVFHTPPRCILNKSKP- 160

Query: 61  KHGGTWLPGSVLNIADCCLQPSEHPNKQDDSVAIVWRDEGFDDSEVNRVTLKQLRQQVML 120
              GTWLP +VLNIA+CCL PS HP K+DDSVA+VWR+EGFDDS VNR+T+K+LR+QVML
Sbjct: 161 --EGTWLPDAVLNIAECCLMPSSHPKKEDDSVAVVWRNEGFDDSPVNRMTIKELREQVML 218

Query: 121 VANAIDATFSKGDAIAIDMQMTVNAVIIYLAIILAGCIVVSIADSFAPKEIATRLRVSKA 180
           VANAI  +F KGD IAIDM MTV+AVIIYLAIILAGCIVVSIADSFA KEIATRL++SKA
Sbjct: 219 VANAISGSFEKGDTIAIDMPMTVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKISKA 278

Query: 181 KGIFTQDFILRGGRKFPLYSRVIEAAACKVIVVPVIGDDVEVQLREQDFSWKGFVSSGS- 239
           KGIFTQD+ILRGGR+FPLYSRV+EAA  KVIV+P  G ++ VQLREQD SW  F+S+   
Sbjct: 279 KGIFTQDYILRGGRRFPLYSRVVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNAKP 338

Query: 240 QKSRSDHYSPIYQPIDSVINILFSSGTTGDPKAIPWTQLAPIRSAADGWAAIDVQAGDVY 299
             S  ++Y PIY P++SVINILFSSGTTG+PKAIPWTQL+PIRSA DGWA +DVQ G  Y
Sbjct: 339 HSSGENYYRPIYLPVESVINILFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGHTY 398

Query: 300 CWPTNLGWVIGPTVLYHCFLTGATLALYHGSPQGRDFGKFVQDAGVTILGTVPSLVKSWK 359
           CWPTNLGWV+GPT+++ CFLTGATLALY GSP GR FGKFVQDAGVT+LGTVPSLVK+WK
Sbjct: 399 CWPTNLGWVMGPTLMFSCFLTGATLALYSGSPLGRGFGKFVQDAGVTVLGTVPSLVKTWK 458

Query: 360 SAKCMEGLDWTKIKSFCSSGETSNVDDDLWLSSKAFYNPIIELCGGTELASSYIAGSPLQ 419
              CMEGL+WTKIK F ++GE SNVDD LWLSSKA Y P+IE CGGTELASSYI GSPLQ
Sbjct: 459 RTNCMEGLNWTKIKFFATTGEASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSPLQ 518

Query: 420 PQAFGACSTASMTTGFVIFDENGVPY 445
           PQAFGA ST SMTT  +IFDENGVPY
Sbjct: 519 PQAFGAFSTPSMTTRIIIFDENGVPY 544


>AT1G55320.2 | Symbols: AAE18 | acyl-activating enzyme 18 |
           chr1:20634194-20636659 FORWARD LENGTH=511
          Length = 511

 Score =  520 bits (1338), Expect = e-147,   Method: Compositional matrix adjust.
 Identities = 250/328 (76%), Positives = 280/328 (85%), Gaps = 1/328 (0%)

Query: 119 MLVANAIDATFSKGDAIAIDMQMTVNAVIIYLAIILAGCIVVSIADSFAPKEIATRLRVS 178
           MLVANAI  +F KGD IAIDM MTV+AVIIYLAIILAGCIVVSIADSFA KEIATRL++S
Sbjct: 1   MLVANAISGSFEKGDTIAIDMPMTVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKIS 60

Query: 179 KAKGIFTQDFILRGGRKFPLYSRVIEAAACKVIVVPVIGDDVEVQLREQDFSWKGFVSSG 238
           KAKGIFTQD+ILRGGR+FPLYSRV+EAA  KVIV+P  G ++ VQLREQD SW  F+S+ 
Sbjct: 61  KAKGIFTQDYILRGGRRFPLYSRVVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNA 120

Query: 239 S-QKSRSDHYSPIYQPIDSVINILFSSGTTGDPKAIPWTQLAPIRSAADGWAAIDVQAGD 297
               S  ++Y PIY P++SVINILFSSGTTG+PKAIPWTQL+PIRSA DGWA +DVQ G 
Sbjct: 121 KPHSSGENYYRPIYLPVESVINILFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGH 180

Query: 298 VYCWPTNLGWVIGPTVLYHCFLTGATLALYHGSPQGRDFGKFVQDAGVTILGTVPSLVKS 357
            YCWPTNLGWV+GPT+++ CFLTGATLALY GSP GR FGKFVQDAGVT+LGTVPSLVK+
Sbjct: 181 TYCWPTNLGWVMGPTLMFSCFLTGATLALYSGSPLGRGFGKFVQDAGVTVLGTVPSLVKT 240

Query: 358 WKSAKCMEGLDWTKIKSFCSSGETSNVDDDLWLSSKAFYNPIIELCGGTELASSYIAGSP 417
           WK   CMEGL+WTKIK F ++GE SNVDD LWLSSKA Y P+IE CGGTELASSYI GSP
Sbjct: 241 WKRTNCMEGLNWTKIKFFATTGEASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSP 300

Query: 418 LQPQAFGACSTASMTTGFVIFDENGVPY 445
           LQPQAFGA ST SMTT  +IFDENGVPY
Sbjct: 301 LQPQAFGAFSTPSMTTRIIIFDENGVPY 328


>AT5G23050.1 | Symbols: AAE17 | acyl-activating enzyme 17 |
           chr5:7731513-7735399 REVERSE LENGTH=721
          Length = 721

 Score =  447 bits (1149), Expect = e-125,   Method: Compositional matrix adjust.
 Identities = 229/447 (51%), Positives = 309/447 (69%), Gaps = 7/447 (1%)

Query: 1   METHASKLLGPSYKDPISSFHLFQKFSVQHPQVYWSLILKELSLSFVEPPRCIL--DTSD 58
           +E    + LG +YK+P+SSF  FQ+FSV +P+VYW  +L EL++ F  PP+CIL  DTS 
Sbjct: 96  LEKRGKEFLGGNYKNPVSSFSSFQEFSVSNPEVYWKTVLDELNILFSVPPKCILEKDTSG 155

Query: 59  PLKHGGTWLPGSVLNIADCCLQPSEHPNKQDDSVAIVWRDEGFDDSEVNRVTLKQLRQQV 118
               GG WLPG+ LN A  CL  +    ++ D + I WRDEG DD  VN +TL +LR QV
Sbjct: 156 D-NPGGKWLPGAYLNPARNCL--TNGFKRRLDDIVIRWRDEGSDDLPVNTMTLLELRSQV 212

Query: 119 MLVANAIDAT-FSKGDAIAIDMQMTVNAVIIYLAIILAGCIVVSIADSFAPKEIATRLRV 177
            L A+A+ A    +  AIA+DM M V +VIIYLAI+LAG +VVSIADSF+P+EI+TRL++
Sbjct: 213 WLAAHALSALGLEEESAIAVDMPMNVESVIIYLAIVLAGHVVVSIADSFSPREISTRLKI 272

Query: 178 SKAKGIFTQDFILRGGRKFPLYSRVIEAAACKVIVVPVIGDDVEVQLREQDFSWKGFVSS 237
           SKAK IFTQD I+RG +  PLY RV++A A   IVVP  G    ++LRE+D SW  F+ +
Sbjct: 273 SKAKAIFTQDVIIRGDKSIPLYRRVVDAEAPLAIVVPARGSSCRMKLREKDLSWNNFLGN 332

Query: 238 GSQKSRSDHYSPIYQPIDSVINILFSSGTTGDPKAIPWTQLAPIRSAADGWAAIDVQAGD 297
            ++  R   Y    +P  +  NILFSSGTTG+PKAIPWT ++P++SAAD W  +DVQ GD
Sbjct: 333 -ARNLRGVEYVAAEKPAGAYTNILFSSGTTGEPKAIPWTNISPLKSAADAWCHLDVQRGD 391

Query: 298 VYCWPTNLGWVIGPTVLYHCFLTGATLALYHGSPQGRDFGKFVQDAGVTILGTVPSLVKS 357
           V  WPTNLGW++GP ++Y   + GA + LY+GSP G  F KFVQDA V++LG +PS+V++
Sbjct: 392 VVAWPTNLGWMMGPWLVYASLINGACMGLYNGSPLGPTFAKFVQDAEVSVLGVIPSIVRT 451

Query: 358 WKSAKCMEGLDWTKIKSFCSSGETSNVDDDLWLSSKAFYNPIIELCGGTELASSYIAGSP 417
           W+++    G DW++I+ F S+GE SN+D+ LWL  +A Y P+IE CGGTE+  S+I+GS 
Sbjct: 452 WQNSNSTSGYDWSRIRCFGSTGEASNIDEYLWLMGRAHYKPVIEYCGGTEIGGSFISGSL 511

Query: 418 LQPQAFGACSTASMTTGFVIFDENGVP 444
           LQPQ+  A STA+M     I DE+  P
Sbjct: 512 LQPQSLAAFSTAAMGCKLFILDEDSNP 538


>AT5G36880.1 | Symbols: ACS | acetyl-CoA synthetase |
           chr5:14534961-14539084 REVERSE LENGTH=693
          Length = 693

 Score = 80.5 bits (197), Expect = 2e-15,   Method: Compositional matrix adjust.
 Identities = 93/359 (25%), Positives = 152/359 (42%), Gaps = 36/359 (10%)

Query: 23  FQKFSVQHPQVYWSLILKELSLSFVEPPRCILDTSDPLKH--GGTWLPGSVLNIADCCLQ 80
             K S+  P  +WS I  E         +   +  D  K      W  G + NI   CL 
Sbjct: 63  MHKRSMDDPAAFWSDIASEFYWKQKWGDQVFSENLDVRKGPISIEWFKGGITNICYNCLD 122

Query: 81  PSEHPNKQDDSVAIVWRDEGFDDSEVNRVTLKQLRQQVMLVANAI-DATFSKGDAIAIDM 139
            +      D + AI W  EG +      +T  +L Q+V  +AN + D    KGDA+ I +
Sbjct: 123 KNVEAGLGDKT-AIHW--EGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYL 179

Query: 140 QMTVNAVIIYLAIILAGCIVVSIADSFAPKEIATRLRVSKAKGIFTQDFILRGGRKFPLY 199
            M +   I  LA    G +   +   F+   +A R+   K   I T + + RG +   L 
Sbjct: 180 PMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLK 239

Query: 200 SRVIEAAACK------VIVVPVIGDDVEVQLRE-------QDFSWKGFVS---SGSQKSR 243
           + +++AA  +       + + +  D+     RE       +D  W+  +S   +  +   
Sbjct: 240 A-IVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVISQYPTSCEVEW 298

Query: 244 SDHYSPIYQPIDSVINILFSSGTTGDPKAIPWTQLA-PIRSAADGWAAIDVQAGDVYCWP 302
            D   P++        +L++SG+TG PK +  T     I +A     A D ++ DVY   
Sbjct: 299 VDAEDPLF--------LLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCT 350

Query: 303 TNLGWVIGPT-VLYHCFLTGATLALYHGSPQGRDFGK---FVQDAGVTILGTVPSLVKS 357
            + GW+ G + V Y   L GAT+ ++ G+P   D G+    V    V+I  T P+LV+S
Sbjct: 351 ADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRS 409


>AT5G36880.2 | Symbols: ACS | acetyl-CoA synthetase |
           chr5:14534961-14540296 REVERSE LENGTH=743
          Length = 743

 Score = 80.1 bits (196), Expect = 3e-15,   Method: Compositional matrix adjust.
 Identities = 93/362 (25%), Positives = 153/362 (42%), Gaps = 36/362 (9%)

Query: 20  FHLFQKFSVQHPQVYWSLILKELSLSFVEPPRCILDTSDPLKH--GGTWLPGSVLNIADC 77
           +    K S+  P  +WS I  E         +   +  D  K      W  G + NI   
Sbjct: 110 YMEMHKRSMDDPAAFWSDIASEFYWKQKWGDQVFSENLDVRKGPISIEWFKGGITNICYN 169

Query: 78  CLQPSEHPNKQDDSVAIVWRDEGFDDSEVNRVTLKQLRQQVMLVANAI-DATFSKGDAIA 136
           CL  +      D + AI W  EG +      +T  +L Q+V  +AN + D    KGDA+ 
Sbjct: 170 CLDKNVEAGLGDKT-AIHW--EGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVV 226

Query: 137 IDMQMTVNAVIIYLAIILAGCIVVSIADSFAPKEIATRLRVSKAKGIFTQDFILRGGRKF 196
           I + M +   I  LA    G +   +   F+   +A R+   K   I T + + RG +  
Sbjct: 227 IYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTI 286

Query: 197 PLYSRVIEAAACK------VIVVPVIGDDVEVQLRE-------QDFSWKGFVS---SGSQ 240
            L + +++AA  +       + + +  D+     RE       +D  W+  +S   +  +
Sbjct: 287 NLKA-IVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVISQYPTSCE 345

Query: 241 KSRSDHYSPIYQPIDSVINILFSSGTTGDPKAIPWTQLA-PIRSAADGWAAIDVQAGDVY 299
               D   P++        +L++SG+TG PK +  T     I +A     A D ++ DVY
Sbjct: 346 VEWVDAEDPLF--------LLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVY 397

Query: 300 CWPTNLGWVIGPT-VLYHCFLTGATLALYHGSPQGRDFGK---FVQDAGVTILGTVPSLV 355
               + GW+ G + V Y   L GAT+ ++ G+P   D G+    V    V+I  T P+LV
Sbjct: 398 WCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLV 457

Query: 356 KS 357
           +S
Sbjct: 458 RS 459