Miyakogusa Predicted Gene

Lj4g3v2573900.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v2573900.1 Non Chatacterized Hit- tr|I1K3P4|I1K3P4_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.53211
PE,86.79,0,seg,NULL; SUBFAMILY NOT NAMED,NULL; FAMILY NOT NAMED,NULL;
MACPF_2,Membrane attack complex component,CUFF.51155.1
         (368 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G14780.1 | Symbols:  | MAC/Perforin domain-containing protein...   320   1e-87
AT4G24290.2 | Symbols:  | MAC/Perforin domain-containing protein...   302   3e-82
AT1G29690.1 | Symbols: CAD1 | MAC/Perforin domain-containing pro...   276   2e-74
AT1G28380.1 | Symbols: NSL1 | MAC/Perforin domain-containing pro...   264   8e-71
AT4G24290.1 | Symbols:  | MAC/Perforin domain-containing protein...    83   4e-16

>AT1G14780.1 | Symbols:  | MAC/Perforin domain-containing protein |
           chr1:5091020-5093873 FORWARD LENGTH=627
          Length = 627

 Score =  320 bits (819), Expect = 1e-87,   Method: Compositional matrix adjust.
 Identities = 159/369 (43%), Positives = 233/369 (63%), Gaps = 12/369 (3%)

Query: 5   TMSFTSISETSSKDGLTIICSKRGGDMLKHSHSSWLQTVPSNPEAILFKFVPISSLLAGI 64
           T++F + S  +S++G+T+IC+KRGGD    SHS WL TVP  P+AI F F+PI+SLL  +
Sbjct: 254 TVAFNNFS-INSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSLLKDV 312

Query: 65  PGSGYLSHAINLYLRYKPPAEDLQYFLEFQIPRQWAPMFCELPLRHHQRRKSSSLSLQFN 124
           PGSG LSHA++LYLRYKPP  DLQYFL+F  PR WAP+  +LP        S+  +L  N
Sbjct: 313 PGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPALHIN 372

Query: 125 FLGPKLHVSSTQVLSEQKPVVGLRLYLEGRKCDRLALHIHHLSSLPNKMILSSETSTPSM 184
           F+GPKL+V++T V SE+ PV G+R +LEG+KC+RLA+H+ HL +   +  +  + +   +
Sbjct: 373 FMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDN--TRTTVGEKITDEHI 430

Query: 185 WRGSDDNESSNQFLEPVRWKRFSNVCTAVVKHDPNWLNSSTG-------VYIVTGAQLVS 237
           WRGSD    ++++ EP+  K+FS+VCT  VK+DPNW+ +++         +IVTGAQL  
Sbjct: 431 WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGAQLEV 490

Query: 238 RCSWPKNVLHLRLLFTNIPNCSIRKTEWAAAP-EASRKXXXXXXXXXXXXXXXXXXXXGP 296
           +    K+VLHLRL +T + +  + +  W   P   S+K                      
Sbjct: 491 KKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVHHNMIQ 550

Query: 297 PKQAPAMLNSGIYPDGPPVPVRAGKLLKYVEAAEVVRGPHDTPGHWLVTAAKLVTEGGKI 356
             +   +L+SG++P GPPVP    K++K+V+ +++ RGP  +PGHWLVT  +L  + GK+
Sbjct: 551 KDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLDKGKL 609

Query: 357 GLQVKFALL 365
            L VKFALL
Sbjct: 610 CLHVKFALL 618


>AT4G24290.2 | Symbols:  | MAC/Perforin domain-containing protein |
           chr4:12594856-12597815 FORWARD LENGTH=606
          Length = 606

 Score =  302 bits (773), Expect = 3e-82,   Method: Compositional matrix adjust.
 Identities = 161/359 (44%), Positives = 227/359 (63%), Gaps = 18/359 (5%)

Query: 9   TSISETSSKDGLTIICSKRGG-DMLKHSHSSWLQTVPSNPEAILFKFVPISSLLAGIPGS 67
           +S+   ++K+    +C +RGG D     H+ WLQTV   P+ I   F+PI+SLL G+PGS
Sbjct: 249 SSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGS 308

Query: 68  GYLSHAINLYLRYKPPAEDLQYFLEFQIPRQWAPMFCELPLRHHQRRKSSSLSLQFNFLG 127
           G+LSHAINLYLRYKPP E+L  FLEFQ+PRQWAP+F ELPL   QR++ S  SLQF+F G
Sbjct: 309 GFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPL-GPQRKQQSCASLQFSFFG 367

Query: 128 PKLHVSSTQVLSEQKPVVGLRLYLEGRKCDRLALHIHHLSSLPNKMILSSETSTPSMWRG 187
           PKL+V++T V   ++P+ G+RLYLEGR+ +RLA+H+ HLSSLP    L  + +  S+ + 
Sbjct: 368 PKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNR-SIRQE 426

Query: 188 SDDNESSNQFLEPVRWKRFSNVCTAVVKHDPNWLNSSTGVYIVTGAQLVSRCSWPKNVLH 247
           S D     ++ E V WK +S+VCT  V+ D +       + +VTGAQL       KNVL 
Sbjct: 427 SHD----RRYYEKVNWKNYSHVCTEPVESDDD-------LSVVTGAQLHVESHGFKNVLF 475

Query: 248 LRLLFTNIPNCS-IRKTEWAAAPEASRKXXXXXXXXXXXXXXXXXXXXGPPKQAPAMLNS 306
           LRL F+ +   + ++ +EW  A   + K                     PP+ A   +NS
Sbjct: 476 LRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKP---PPRPADVNINS 532

Query: 307 GIYPDGPPVPVRAGKLLKYVEAAEVVRGPHDTPGHWLVTAAKLVTEGGKIGLQVKFALL 365
            IYP GPPVP +A KLLK+V+ +E+ RGP ++PG+W+V+ A+L+ E GKI L+VK++L 
Sbjct: 533 AIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSLF 591


>AT1G29690.1 | Symbols: CAD1 | MAC/Perforin domain-containing
           protein | chr1:10379310-10381861 REVERSE LENGTH=561
          Length = 561

 Score =  276 bits (705), Expect = 2e-74,   Method: Compositional matrix adjust.
 Identities = 156/369 (42%), Positives = 221/369 (59%), Gaps = 36/369 (9%)

Query: 2   QSNTMSFTSISETSSKDGLTIICSKRGGDMLKHSHSSWLQTVPSNPEAILFKFVPISSLL 61
           ++ + S T   +   KD +T+I  +RGGD L+ SH+ W +TVP+ P+ I   F PI SLL
Sbjct: 226 EAESQSITGPLKYKDKD-ITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLL 284

Query: 62  AGIPGSGYLSHAINLYLRYKPPAEDLQYFLEFQIPRQWAPMFCELPLRHHQRRKSSSLSL 121
            G+PG  +L+ AI LYL YKPP EDLQYFL++QI R WAP    L     QR++    SL
Sbjct: 285 EGVPGLRHLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL-----QRKEPVCSSL 339

Query: 122 QFNFLGPKLHVSSTQVLSEQKPVVGLRLYLEGRKCDRLALHIHHLSSLPNKMILSSETST 181
           QF+ +GPKL +S+ QV   +KPV GLRL LEG K +RL++H+ HL SLP  +    ++  
Sbjct: 340 QFSLMGPKLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHV 399

Query: 182 P---SMWRGSDDNESSNQFLEPVRWKRFSNVCTAVVKHDPNWLNSSTGVYIVTGAQLVSR 238
           P     W+G ++ +S  ++ EP++WK FS+V T+ ++H    +   +GV+IVTGAQL   
Sbjct: 400 PIGAPKWQGPEEQDS--RWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVW 457

Query: 239 CSWPKNVLHLRLLFTNIPNCSIRKTEWAAAPEASRKXXXXXXXXXXXXXXXXXXXXGPPK 298
               KNVLHL+LLF+ +P C+IR++ W   P AS                      GP  
Sbjct: 458 DFGSKNVLHLKLLFSKVPGCTIRRSVWDHTPVAS---------------SGRLEPGGPST 502

Query: 299 QAPAMLNSGIYPDGPPVPVRAGKLLKYVEAAEVVRGPHDTPGHWLVTAAKLVTEGGKIGL 358
            +     SG          ++GKL K V+++E+++GP D PGHWLVT AKL  E GKI L
Sbjct: 503 SSSTEEVSG----------QSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVL 552

Query: 359 QVKFALLDY 367
           +VK++LL+Y
Sbjct: 553 RVKYSLLNY 561


>AT1G28380.1 | Symbols: NSL1 | MAC/Perforin domain-containing
           protein | chr1:9963696-9966060 FORWARD LENGTH=612
          Length = 612

 Score =  264 bits (674), Expect = 8e-71,   Method: Compositional matrix adjust.
 Identities = 147/343 (42%), Positives = 204/343 (59%), Gaps = 9/343 (2%)

Query: 23  ICSKRGGDMLKHSHSSWLQTVPSNPEAILFKFVPISSLLAGIPGSGYLSHAINLYLRYKP 82
           +C +RGG  L  SH  WL TV   P  I   FVPI+SLL+G+PG+G+LSHA+NLYLRYKP
Sbjct: 270 VCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPGTGFLSHAVNLYLRYKP 329

Query: 83  PAEDLQYFLEFQIPRQWAPMFCELPLRHHQRRKSSSLSLQFNFLGPKLHVSSTQVLSEQK 142
           P E+L  FLEFQ+PRQWAP++ +LPL   +R K SS SLQF+ +GPKL+V++++V S ++
Sbjct: 330 PIEELHQFLEFQLPRQWAPVYGDLPL-GLRRSKQSSPSLQFSLMGPKLYVNTSKVDSGER 388

Query: 143 PVVGLRLYLEGRKCDRLALHIHHLSSLPNKMILSSETSTPSMWRGSDDNESSNQFLEPVR 202
           PV GLR +LEG+K + LA+H+ HLS+ P  + LS + +   +     +      +  PV+
Sbjct: 389 PVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPI-----EEPVEKGYYVPVK 443

Query: 203 WKRFSNVCTAVVKHDPNWLNSSTGVYIVTGAQLVSRCSWPKNVLHLRLLFTNIPNCSIRK 262
           W  FS+VCT  V++  N   S     IVT A L  +    + VL LRL F+   +   RK
Sbjct: 444 WGIFSHVCTYPVQY--NGARSDDTASIVTKAWLEVKGMGMRKVLFLRLGFSLDASAVTRK 501

Query: 263 TEWAAAPEASRKXXXXXXXXXXXXXXXXXXXXGPPKQAPAMLNSGIYPDGPPVPVRAGKL 322
           + W      SRK                        Q+   +NS +YP GP  PV+  KL
Sbjct: 502 SCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKIDINSAVYPRGPSPPVKP-KL 560

Query: 323 LKYVEAAEVVRGPHDTPGHWLVTAAKLVTEGGKIGLQVKFALL 365
           L  V+  EV+RGP + PG+W+VT AKL  E GKI ++ K++LL
Sbjct: 561 LSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 603


>AT4G24290.1 | Symbols:  | MAC/Perforin domain-containing protein |
           chr4:12594856-12596590 FORWARD LENGTH=350
          Length = 350

 Score = 82.8 bits (203), Expect = 4e-16,   Method: Compositional matrix adjust.
 Identities = 41/88 (46%), Positives = 56/88 (63%), Gaps = 3/88 (3%)

Query: 9   TSISETSSKDGLTIICSKRGG-DMLKHSHSSWLQTVPSNPEAILFKFVPISSLLAGIPGS 67
           +S+   ++K+    +C +RGG D     H+ WLQTV   P+ I   F+PI+SLL G+PGS
Sbjct: 249 SSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGS 308

Query: 68  GYLSHAINLYLRYKPP--AEDLQYFLEF 93
           G+LSHAINLYLR KP      + +FLE 
Sbjct: 309 GFLSHAINLYLRCKPINHLRFVHFFLEL 336