Miyakogusa Predicted Gene

Lj4g3v2468350.2
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v2468350.2 Non Chatacterized Hit- tr|I3SEX4|I3SEX4_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2
SV=1,98.99,0,Transketolase, pyrimidine binding
domain,Transketolase-like, pyrimidine-binding domain; Thiamin
diph,CUFF.51053.2
         (400 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G34590.1 | Symbols:  | Transketolase family protein | chr2:14...   595   e-170
AT1G30120.1 | Symbols: PDH-E1 BETA | pyruvate dehydrogenase E1 b...   593   e-170
AT5G50850.1 | Symbols: MAB1 | Transketolase family protein | chr...   240   1e-63
AT1G55510.1 | Symbols: BCDH BETA1 | branched-chain alpha-keto ac...   184   1e-46
AT3G13450.1 | Symbols: DIN4 | Transketolase family protein | chr...   182   3e-46

>AT2G34590.1 | Symbols:  | Transketolase family protein |
           chr2:14568956-14570844 REVERSE LENGTH=406
          Length = 406

 Score =  595 bits (1534), Expect = e-170,   Method: Compositional matrix adjust.
 Identities = 290/351 (82%), Positives = 306/351 (87%), Gaps = 1/351 (0%)

Query: 51  ARKHDLLITNAVATKEGSSVASTS-KPGHXXXXXXXXXXXXXXXXXRDPNVCVMGEDVGH 109
           AR+ + LI NAV TK  ++ +STS KPGH                 RDP+VCVMGEDVGH
Sbjct: 56  ARRSEPLIPNAVTTKADTAASSTSSKPGHELLLFEALQEGLEEEMDRDPHVCVMGEDVGH 115

Query: 110 YGGSYKVTKDLANKFGDLRVLDTPIAENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAFNQ 169
           YGGSYKVTK LA+KFGDLRVLDTPI ENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAFNQ
Sbjct: 116 YGGSYKVTKGLADKFGDLRVLDTPICENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAFNQ 175

Query: 170 ISNNCGMLHYTSGGQFKXXXXXXXXXXXXXQLGAEHSQRLESYFQSIPGIQMVACSTPYN 229
           ISNNCGMLHYTSGGQF              QLGAEHSQRLESYFQSIPGIQMVACSTPYN
Sbjct: 176 ISNNCGMLHYTSGGQFTIPVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACSTPYN 235

Query: 230 AKGLMKAAIRSDNPVILFEHVLLYNLKERIPDEEYVLSLEEAEMVRPGEHVTILTYSRMR 289
           AKGLMKAAIRS+NPVILFEHVLLYNLKE IPDEEY+ +LEEAEMVRPGEH+TILTYSRMR
Sbjct: 236 AKGLMKAAIRSENPVILFEHVLLYNLKESIPDEEYICNLEEAEMVRPGEHITILTYSRMR 295

Query: 290 YHVMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRVLIVEECMRTGGIGASLT 349
           YHVMQAAKTLVNKGYDPEVIDIRSLKPFDL+TIGNSVKKTHRVLIVEECMRTGGIGASLT
Sbjct: 296 YHVMQAAKTLVNKGYDPEVIDIRSLKPFDLYTIGNSVKKTHRVLIVEECMRTGGIGASLT 355

Query: 350 AAISENFNDYLDAPIVCLSSQDVPTPYAGPLEEMTVVQPAQIVTAVEQLCQ 400
           AAI+ENF+DYLDAP++CLSSQDVPTPYAG LEE TVVQPAQIVTAVEQLCQ
Sbjct: 356 AAINENFHDYLDAPVMCLSSQDVPTPYAGTLEEWTVVQPAQIVTAVEQLCQ 406


>AT1G30120.1 | Symbols: PDH-E1 BETA | pyruvate dehydrogenase E1 beta
           | chr1:10584350-10586477 REVERSE LENGTH=406
          Length = 406

 Score =  593 bits (1530), Expect = e-170,   Method: Compositional matrix adjust.
 Identities = 295/367 (80%), Positives = 308/367 (83%), Gaps = 7/367 (1%)

Query: 38  FVVRSDARANQTGA----RKHDLLITNAVATKEGSSVASTSKPGHXXXXXXXXXXXXXXX 93
            V  SDA     G+    R    LI NAVATKE  + AST   GH               
Sbjct: 43  IVAGSDASKKSFGSGLRVRHSQKLIPNAVATKEADTSAST---GHELLLFEALQEGLEEE 99

Query: 94  XXRDPNVCVMGEDVGHYGGSYKVTKDLANKFGDLRVLDTPIAENAFTGMGIGAAMTGLRP 153
             RDP+VCVMGEDVGHYGGSYKVTK LA+KFGDLRVLDTPI ENAFTGMGIGAAMTGLRP
Sbjct: 100 MDRDPHVCVMGEDVGHYGGSYKVTKGLADKFGDLRVLDTPICENAFTGMGIGAAMTGLRP 159

Query: 154 VIEGMNMGFLLLAFNQISNNCGMLHYTSGGQFKXXXXXXXXXXXXXQLGAEHSQRLESYF 213
           VIEGMNMGFLLLAFNQISNNCGMLHYTSGGQF              QLGAEHSQRLESYF
Sbjct: 160 VIEGMNMGFLLLAFNQISNNCGMLHYTSGGQFTIPVVIRGPGGVGRQLGAEHSQRLESYF 219

Query: 214 QSIPGIQMVACSTPYNAKGLMKAAIRSDNPVILFEHVLLYNLKERIPDEEYVLSLEEAEM 273
           QSIPGIQMVACSTPYNAKGLMKAAIRS+NPVILFEHVLLYNLKE+IPDE+YV +LEEAEM
Sbjct: 220 QSIPGIQMVACSTPYNAKGLMKAAIRSENPVILFEHVLLYNLKEKIPDEDYVCNLEEAEM 279

Query: 274 VRPGEHVTILTYSRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRVL 333
           VRPGEH+TILTYSRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRVL
Sbjct: 280 VRPGEHITILTYSRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRVL 339

Query: 334 IVEECMRTGGIGASLTAAISENFNDYLDAPIVCLSSQDVPTPYAGPLEEMTVVQPAQIVT 393
           IVEECMRTGGIGASLTAAI+ENF+DYLDAP++CLSSQDVPTPYAG LEE TVVQPAQIVT
Sbjct: 340 IVEECMRTGGIGASLTAAINENFHDYLDAPVMCLSSQDVPTPYAGTLEEWTVVQPAQIVT 399

Query: 394 AVEQLCQ 400
           AVEQLCQ
Sbjct: 400 AVEQLCQ 406


>AT5G50850.1 | Symbols: MAB1 | Transketolase family protein |
           chr5:20689671-20692976 FORWARD LENGTH=363
          Length = 363

 Score =  240 bits (613), Expect = 1e-63,   Method: Compositional matrix adjust.
 Identities = 124/307 (40%), Positives = 185/307 (60%), Gaps = 4/307 (1%)

Query: 97  DPNVCVMGEDVGHYGGSYKVTKDLANKFGDLRVLDTPIAENAFTGMGIGAAMTGLRPVIE 156
           DP V VMGE+VG Y G+YK+TK L  K+G  RV DTPI E  FTG+G+GAA  GL+PV+E
Sbjct: 53  DPKVFVMGEEVGQYQGAYKITKGLLEKYGPERVYDTPITEAGFTGIGVGAAYAGLKPVVE 112

Query: 157 GMNMGFLLLAFNQISNNCGMLHYTSGGQFKXXXXXXXXXXXXXQLGAEHSQRLESYFQSI 216
            M   F + A + I N+    +Y S GQ                +GA+HSQ   +++ S+
Sbjct: 113 FMTFNFSMQAIDHIINSAAKSNYMSAGQINVPIVFRGPNGAAAGVGAQHSQCYAAWYASV 172

Query: 217 PGIQMVACSTPYNAKGLMKAAIRSDNPVILFEHVLLYN----LKERIPDEEYVLSLEEAE 272
           PG++++A  +  +A+GL+KAAIR  +PV+  E+ LLY     + E   D  + L + +A+
Sbjct: 173 PGLKVLAPYSAEDARGLLKAAIRDPDPVVFLENELLYGESFPISEEALDSSFCLPIGKAK 232

Query: 273 MVRPGEHVTILTYSRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRV 332
           + R G+ VTI+T+S+M    ++AA+ L  +G   EVI++RS++P D  TI  SV+KT R+
Sbjct: 233 IEREGKDVTIVTFSKMVGFALKAAEKLAEEGISAEVINLRSIRPLDRATINASVRKTSRL 292

Query: 333 LIVEECMRTGGIGASLTAAISENFNDYLDAPIVCLSSQDVPTPYAGPLEEMTVVQPAQIV 392
           + VEE     G+ A + A++ E    YLDAP+  ++  DVP PYA  LE + + Q   IV
Sbjct: 293 VTVEEGFPQHGVCAEICASVVEESFSYLDAPVERIAGADVPMPYAANLERLALPQIEDIV 352

Query: 393 TAVEQLC 399
            A ++ C
Sbjct: 353 RASKRAC 359


>AT1G55510.1 | Symbols: BCDH BETA1 | branched-chain alpha-keto acid
           decarboxylase E1 beta subunit | chr1:20723482-20725505
           FORWARD LENGTH=352
          Length = 352

 Score =  184 bits (466), Expect = 1e-46,   Method: Compositional matrix adjust.
 Identities = 105/281 (37%), Positives = 150/281 (53%), Gaps = 3/281 (1%)

Query: 97  DPNVCVMGEDVGHYGGSYKVTKDLANKFGDLRVLDTPIAENAFTGMGIGAAMTGLRPVIE 156
           DP   V GEDVG +GG ++ T  LA +FG  RV +TP+ E    G GIG A  G R ++E
Sbjct: 49  DPRSYVFGEDVG-FGGVFRCTTGLAERFGKNRVFNTPLCEQGIVGFGIGLAAMGNRAIVE 107

Query: 157 GMNMGFLLLAFNQISNNCGMLHYTSGGQFKXXXXXXXXXXXXXQLGAE-HSQRLESYFQS 215
                ++  AF+QI N      Y SG QF                G   HSQ  E++F  
Sbjct: 108 IQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH 167

Query: 216 IPGIQMVACSTPYNAKGLMKAAIRSDNPVILFEHVLLYNLK-ERIPDEEYVLSLEEAEMV 274
           +PGI++V   +P  AKGL+ + IR  NPV+ FE   LY    E +P+ +Y++ L EAE++
Sbjct: 168 VPGIKVVIPRSPREAKGLLLSCIRDPNPVVFFEPKWLYRQAVEEVPEHDYMIPLSEAEVI 227

Query: 275 RPGEHVTILTYSRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRVLI 334
           R G  +T++ +      + QA      +G   E+ID+++L P+D  T+  SVKKT R+LI
Sbjct: 228 REGNDITLVGWGAQLTVMEQACLDAEKEGISCELIDLKTLLPWDKETVEASVKKTGRLLI 287

Query: 335 VEECMRTGGIGASLTAAISENFNDYLDAPIVCLSSQDVPTP 375
             E   TGG GA ++A I E     L+AP+  +   D P P
Sbjct: 288 SHEAPVTGGFGAEISATILERCFLKLEAPVSRVCGLDTPFP 328


>AT3G13450.1 | Symbols: DIN4 | Transketolase family protein |
           chr3:4382340-4384295 REVERSE LENGTH=358
          Length = 358

 Score =  182 bits (462), Expect = 3e-46,   Method: Compositional matrix adjust.
 Identities = 104/281 (37%), Positives = 150/281 (53%), Gaps = 3/281 (1%)

Query: 97  DPNVCVMGEDVGHYGGSYKVTKDLANKFGDLRVLDTPIAENAFTGMGIGAAMTGLRPVIE 156
           DP   V GEDVG +GG ++ T  LA +FG  RV +TP+ E    G GIG A  G R + E
Sbjct: 55  DPRSYVFGEDVG-FGGVFRCTTGLAERFGKSRVFNTPLCEQGIVGFGIGLAAMGNRVIAE 113

Query: 157 GMNMGFLLLAFNQISNNCGMLHYTSGGQFKXXXXXXXXXXXXXQLGAE-HSQRLESYFQS 215
                ++  AF+QI N      Y SG QF                G   HSQ  E++F  
Sbjct: 114 IQFADYIFPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCH 173

Query: 216 IPGIQMVACSTPYNAKGLMKAAIRSDNPVILFEHVLLYNLK-ERIPDEEYVLSLEEAEMV 274
           +PGI++V   +P  AKGL+ ++IR  NPV+ FE   LY    E +P+++Y++ L EAE++
Sbjct: 174 VPGIKVVIPRSPREAKGLLLSSIRDPNPVVFFEPKWLYRQAVEDVPEDDYMIPLSEAEVM 233

Query: 275 RPGEHVTILTYSRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRVLI 334
           R G  +T++ +      + QA     N+G   E+ID+++L P+D   +  SV+KT R+LI
Sbjct: 234 REGSDITLVGWGAQLTIMEQACLDAENEGISCELIDLKTLIPWDKEIVETSVRKTGRLLI 293

Query: 335 VEECMRTGGIGASLTAAISENFNDYLDAPIVCLSSQDVPTP 375
             E   TGG GA + A I E     L+AP+  +   D P P
Sbjct: 294 SHEAPVTGGFGAEIAATIVERCFLRLEAPVSRVCGLDTPFP 334