Miyakogusa Predicted Gene

Lj4g3v0877830.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v0877830.1 tr|I1L9S4|I1L9S4_SOYBN DNA topoisomerase 2
OS=Glycine max GN=Gma.48179 PE=3 SV=1,59.4,0,DNA_topoisoIV,DNA
topoisomerase, type IIA, subunit A/C-terminal; TOPISMRASEII,DNA
topoisomerase II, ,CUFF.48145.1
         (369 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G23890.1 | Symbols: TOPII, ATTOPII | topoisomerase II | chr3:...   421   e-118
AT3G23890.2 | Symbols: TOPII, ATTOPII | topoisomerase II | chr3:...   421   e-118
AT3G10690.1 | Symbols: GYRA | DNA GYRASE A | chr3:3339612-334624...    57   2e-08

>AT3G23890.1 | Symbols: TOPII, ATTOPII | topoisomerase II |
           chr3:8624931-8631106 FORWARD LENGTH=1473
          Length = 1473

 Score =  421 bits (1083), Expect = e-118,   Method: Compositional matrix adjust.
 Identities = 201/367 (54%), Positives = 269/367 (73%), Gaps = 2/367 (0%)

Query: 1   MESFIYGRVMFMADQDYDGSHIKGLLINFFYMLSPSLLRIPYFLSELITPIIRAFHCNGI 60
           + S  YG++M M DQD+DGSHIKGLLINF +   PSLL++P FL E ITPI++A      
Sbjct: 525 VNSLRYGQMMIMTDQDHDGSHIKGLLINFIHSFWPSLLQVPSFLVEFITPIVKATRKGTK 584

Query: 61  QKLSFYSYPEYEKWWKNW-GNSD-WRIKYYKGLGSFTHEEGKEFFQNVEIQRKYFVWRND 118
           + LSFYS PEYE+W ++  GN+  W IKYYKGLG+ T EEGKE+F N+ + +K FVW ++
Sbjct: 585 KVLSFYSMPEYEEWKESLKGNATGWDIKYYKGLGTSTAEEGKEYFSNLGLHKKDFVWEDE 644

Query: 119 EDLDAINIAFSKSKTRERRGLMLKFKSSDYRDYTDLSICYKDFLQKEFILYSMASVRRCI 178
           +D +AI +AFSK K   R+  +  +   ++ D     + Y DF+ KE IL+SMA ++R I
Sbjct: 645 QDGEAIELAFSKKKIEARKNWLSSYVPGNHLDQRQPKVTYSDFVNKELILFSMADLQRSI 704

Query: 179 PSLIDGQKPSQRKILYGAFKRELFTEIRVDKLIGYVAEHSAYHHDESSLATTLMRMARDY 238
           PS++DG KP QRKIL+ AFK+    E++V +L+GYV+  SAYHH E SLA+ ++ MA+DY
Sbjct: 705 PSMVDGLKPGQRKILFVAFKKIARKEMKVAQLVGYVSLLSAYHHGEQSLASAIIGMAQDY 764

Query: 239 VGSMNINLLMANGQFGTHIQGGKDHASPRYIYTELNPISRFLFPEIDNKLLKYLNEDGIS 298
           VGS NINLL+ NGQFGT   GGKD AS RYI+T+L+P++R LFP+ D+ LL YLNEDG  
Sbjct: 765 VGSNNINLLLPNGQFGTRTSGGKDSASARYIFTKLSPVTRILFPKDDDLLLDYLNEDGQR 824

Query: 299 VEPEWYLPIIPLVLVNGVSGIATGWNCSIPCYNPKDIIANIKRFLNDDKLQPMVPWYKGF 358
           +EP WY+PIIP VLVNG  GI TGW+  IP YNP++I+AN++R LN + + PM PWY+GF
Sbjct: 825 IEPTWYMPIIPTVLVNGAEGIGTGWSTFIPNYNPREIVANVRRLLNGESMVPMDPWYRGF 884

Query: 359 KGTIDKS 365
           KGTI+K+
Sbjct: 885 KGTIEKT 891


>AT3G23890.2 | Symbols: TOPII, ATTOPII | topoisomerase II |
           chr3:8624931-8631106 FORWARD LENGTH=1438
          Length = 1438

 Score =  421 bits (1083), Expect = e-118,   Method: Compositional matrix adjust.
 Identities = 201/367 (54%), Positives = 269/367 (73%), Gaps = 2/367 (0%)

Query: 1   MESFIYGRVMFMADQDYDGSHIKGLLINFFYMLSPSLLRIPYFLSELITPIIRAFHCNGI 60
           + S  YG++M M DQD+DGSHIKGLLINF +   PSLL++P FL E ITPI++A      
Sbjct: 525 VNSLRYGQMMIMTDQDHDGSHIKGLLINFIHSFWPSLLQVPSFLVEFITPIVKATRKGTK 584

Query: 61  QKLSFYSYPEYEKWWKNW-GNSD-WRIKYYKGLGSFTHEEGKEFFQNVEIQRKYFVWRND 118
           + LSFYS PEYE+W ++  GN+  W IKYYKGLG+ T EEGKE+F N+ + +K FVW ++
Sbjct: 585 KVLSFYSMPEYEEWKESLKGNATGWDIKYYKGLGTSTAEEGKEYFSNLGLHKKDFVWEDE 644

Query: 119 EDLDAINIAFSKSKTRERRGLMLKFKSSDYRDYTDLSICYKDFLQKEFILYSMASVRRCI 178
           +D +AI +AFSK K   R+  +  +   ++ D     + Y DF+ KE IL+SMA ++R I
Sbjct: 645 QDGEAIELAFSKKKIEARKNWLSSYVPGNHLDQRQPKVTYSDFVNKELILFSMADLQRSI 704

Query: 179 PSLIDGQKPSQRKILYGAFKRELFTEIRVDKLIGYVAEHSAYHHDESSLATTLMRMARDY 238
           PS++DG KP QRKIL+ AFK+    E++V +L+GYV+  SAYHH E SLA+ ++ MA+DY
Sbjct: 705 PSMVDGLKPGQRKILFVAFKKIARKEMKVAQLVGYVSLLSAYHHGEQSLASAIIGMAQDY 764

Query: 239 VGSMNINLLMANGQFGTHIQGGKDHASPRYIYTELNPISRFLFPEIDNKLLKYLNEDGIS 298
           VGS NINLL+ NGQFGT   GGKD AS RYI+T+L+P++R LFP+ D+ LL YLNEDG  
Sbjct: 765 VGSNNINLLLPNGQFGTRTSGGKDSASARYIFTKLSPVTRILFPKDDDLLLDYLNEDGQR 824

Query: 299 VEPEWYLPIIPLVLVNGVSGIATGWNCSIPCYNPKDIIANIKRFLNDDKLQPMVPWYKGF 358
           +EP WY+PIIP VLVNG  GI TGW+  IP YNP++I+AN++R LN + + PM PWY+GF
Sbjct: 825 IEPTWYMPIIPTVLVNGAEGIGTGWSTFIPNYNPREIVANVRRLLNGESMVPMDPWYRGF 884

Query: 359 KGTIDKS 365
           KGTI+K+
Sbjct: 885 KGTIEKT 891


>AT3G10690.1 | Symbols: GYRA | DNA GYRASE A | chr3:3339612-3346243
           REVERSE LENGTH=950
          Length = 950

 Score = 57.0 bits (136), Expect = 2e-08,   Method: Compositional matrix adjust.
 Identities = 46/165 (27%), Positives = 84/165 (50%), Gaps = 8/165 (4%)

Query: 176 RCIPSLIDGQKPSQRKILYGAFKRELFTEIRVDKLIGYVAE-HSAYH-HDESSLATTLMR 233
           R +P + DG KP  R+IL+   +  + ++    K    V E    +H H ++++  +L+R
Sbjct: 125 RALPDVRDGLKPVHRRILFAMHELGMSSKKPYKKCARVVGEVLGKFHPHGDTAVYDSLVR 184

Query: 234 MARDYVGSMNINLLMANGQFGTHIQGGKDHASPRYIYTELNPISR-FLFPEIDNKLLKYL 292
           MA+ +  S+   L+  +G FG+        A+ RY    L+P++   L  ++D   + ++
Sbjct: 185 MAQSF--SLRCPLIQGHGNFGSI--DADPPAAMRYTECRLDPLAEAVLLSDLDQDTVDFV 240

Query: 293 -NEDGISVEPEWYLPIIPLVLVNGVSGIATGWNCSIPCYNPKDII 336
            N D    EP      +P +L+NG SGIA G   +IP +N  +++
Sbjct: 241 ANFDNSQKEPAVLPARLPALLLNGASGIAVGMATNIPPHNLGELV 285