Miyakogusa Predicted Gene

Lj4g3v0473110.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v0473110.1 Non Chatacterized Hit- tr|I1M079|I1M079_SOYBN
Uncharacterized protein OS=Glycine max PE=4
SV=1,79.97,0,MACPF,Membrane attack complex component/perforin (MACPF)
domain; membrane-attack complex / perforin,,CUFF.47341.1
         (619 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G28380.1 | Symbols: NSL1 | MAC/Perforin domain-containing pro...   724   0.0  
AT4G24290.2 | Symbols:  | MAC/Perforin domain-containing protein...   589   e-168
AT1G14780.1 | Symbols:  | MAC/Perforin domain-containing protein...   417   e-116
AT1G29690.1 | Symbols: CAD1 | MAC/Perforin domain-containing pro...   391   e-109
AT4G24290.1 | Symbols:  | MAC/Perforin domain-containing protein...   313   3e-85

>AT1G28380.1 | Symbols: NSL1 | MAC/Perforin domain-containing
           protein | chr1:9963696-9966060 FORWARD LENGTH=612
          Length = 612

 Score =  724 bits (1870), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 364/617 (58%), Positives = 453/617 (73%), Gaps = 27/617 (4%)

Query: 17  LDPQLAAEKAVSVIGQGYDLCTDIRFSSCK-----SRLVEIDGNRTRDLVLPAGVVVPDV 71
           LD   AAEKAVSVIG GYDLC+D+RFS+CK     SRLVEID  R RDL+ P G+VV +V
Sbjct: 9   LDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNV 68

Query: 72  PSSIKCDKGERTRFRSDVLPFNQMSEHFNQQLSLSGKIPSGQFNKMFDMRKGWSRDAAST 131
            SSIKCDKGERTR RSD+L FNQMSE FNQ + LSGKIPSG FN MF   K W +DA+S 
Sbjct: 69  SSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSV 128

Query: 132 KNLAYDGWFITLYTVELDRTKITLSENVKKEVPSSWNPAALTEFIEKYGTHIXXXXXXXX 191
           K LAYDGWFI+LY+VE+ R ++TL + VK+EVPSSW+ AAL  FIEKYGTH+        
Sbjct: 129 KTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGG 188

Query: 192 XXXXHIKQSKSSDLQPTELQKLLKQLADEGFSEDSNRSSIVNPIET-------SGKRKDG 244
               H+KQ + S+ +P E+QK+LK   DE F         V+P+E+       SGK K+ 
Sbjct: 189 KDVIHVKQMRKSNHEPEEIQKMLKHWGDERF--------CVDPVESKSPASVYSGKPKEE 240

Query: 245 HAKLLGQYQYKPSLVGRPVVRSHSKNDDITSISVRRGGIDTCQSYNQWLSTISESPNVIS 304
           +    G   +  S+    V+  H+KN++I  + +RRGG+D  QS+ +WLST+S++PNVIS
Sbjct: 241 NLLQWGLQPFGTSVSSAVVM--HTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVIS 298

Query: 305 MSFVPITSLLNSVPGNGFLSHAMNLYLRYKPAIEELHQFLEFQLPRQWAPMYDHLPLGFG 364
           M FVPITSLL+ +PG GFLSHA+NLYLRYKP IEELHQFLEFQLPRQWAP+Y  LPLG  
Sbjct: 299 MCFVPITSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGL- 357

Query: 365 HKFKRNMSPSLQFTLMGPKLFVNTVKVDSENRPVTGIRLYLEGRRNNHLSIHLQHLSEVP 424
            +  +  SPSLQF+LMGPKL+VNT KVDS  RPVTG+R +LEG++ NHL+IHLQHLS  P
Sbjct: 358 -RRSKQSSPSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACP 416

Query: 425 GVLEISEDHGYDPIDEPDDRGYYEPVKWSMFSHVYTAPVQYNSSRIDESTEIVTKAWFEV 484
             L +S D  Y+PI+EP ++GYY PVKW +FSHV T PVQYN +R D++  IVTKAW EV
Sbjct: 417 PSLHLSHDDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGARSDDTASIVTKAWLEV 476

Query: 485 KVVGMKKVLFLRLGFSTVASAKIRRSEWDGPSTSSRKSGFFSALMS--SKKLNKEVQSPE 542
           K +GM+KVLFLRLGFS  ASA  R+S WD  ST+SRKSG FS + +  S  L+    + +
Sbjct: 477 KGMGMRKVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTK 536

Query: 543 GPTKVDVNSAIYHDGPPVPRKTPKMLSFVDTQEMVRGPEDPPGYWVVTGAKLCVEGGRIS 602
             +K+D+NSA+Y  GP  P K PK+LS VDT+E++RGPE+ PGYWVVTGAKLCVE G+IS
Sbjct: 537 PQSKIDINSAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKIS 595

Query: 603 IKAKYSLLTILSEEFLL 619
           IKAKYSLLT++SE+ L+
Sbjct: 596 IKAKYSLLTVISEDSLV 612


>AT4G24290.2 | Symbols:  | MAC/Perforin domain-containing protein |
           chr4:12594856-12597815 FORWARD LENGTH=606
          Length = 606

 Score =  589 bits (1518), Expect = e-168,   Method: Compositional matrix adjust.
 Identities = 312/614 (50%), Positives = 403/614 (65%), Gaps = 24/614 (3%)

Query: 14  GLELDPQLAAEKAVSVIGQGYDLCTDIRFSSCK-----SRLVEI-DGNRTRDLVLPAGVV 67
            L L    AAE A+  IG GYDL  D+R   CK     SRL++I +G+   ++VLP G+ 
Sbjct: 2   ALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGIS 61

Query: 68  VPDVPSSIKCDKGERTRFRSDVLPFNQMSEHFNQQLSLSGKIPSGQFNKMFDMRKGWSRD 127
           +P+V  SIKCDKGER RFRSD+LPF QM+E FNQ+LSL+GKIPSG FN MF+    W +D
Sbjct: 62  IPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKD 121

Query: 128 AASTKNLAYDGWFITLYTVELDRTKITLSENVKKEVPSSWNPAALTEFIEKYGTHIXXXX 187
           AA TKNLA+DG FI+LY+V LD++++ L E+VK+ VPS+W+PAAL  FI+ YGTHI    
Sbjct: 122 AAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSV 181

Query: 188 XXXXXXXXHIKQSKSSDLQPTELQKLLKQLADEGFSEDSNRSSIVNPIETSGKRKDGHAK 247
                   + KQ  SS LQP +LQK LK++AD+ F E    +S+V+   T  +R    +K
Sbjct: 182 KMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVE----ASVVH--NTGSERVQASSK 235

Query: 248 LLGQYQYKPSLVGRPVVRSHSKNDDITSISVRRGGIDTCQ-SYNQWLSTISESPNVISMS 306
           +  + Q +        + S++  +D   +  RRGG D     +N+WL T+   P+VISMS
Sbjct: 236 VETKEQ-RLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMS 294

Query: 307 FVPITSLLNSVPGNGFLSHAMNLYLRYKPAIEELHQFLEFQLPRQWAPMYDHLPLGFGHK 366
           F+PITSLLN VPG+GFLSHA+NLYLRYKP IEELHQFLEFQLPRQWAP++  LPL  G +
Sbjct: 295 FIPITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPL--GPQ 352

Query: 367 FKRNMSPSLQFTLMGPKLFVNTVKVDSENRPVTGIRLYLEGRRNNHLSIHLQHLSEVPGV 426
            K+    SLQF+  GPKL+VNT  VD   RP+TG+RLYLEGRR+N L+IHLQHLS +P +
Sbjct: 353 RKQQSCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKI 412

Query: 427 LEISEDHGYDPIDEPDDRGYYEPVKWSMFSHVYTAPVQYNSSRIDESTEIVTKAWFEVKV 486
            ++ +D       E  DR YYE V W  +SHV T PV+      D+   +VT A   V+ 
Sbjct: 413 YQLEDDLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVES-----DDDLSVVTGAQLHVES 467

Query: 487 VGMKKVLFLRLGFSTVASAK-IRRSEWDGPSTSSRKSGFFSALMSSKKLNKEVQSPEGPT 545
            G K VLFLRL FS V  A  ++ SEWD     + KSG  S L+ S       + P  P 
Sbjct: 468 HGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLI-SHHFTAAQKPPPRPA 526

Query: 546 KVDVNSAIYHDGPPVPRKTPKMLSFVDTQEMVRGPEDPPGYWVVTGAKLCVEGGRISIKA 605
            V++NSAIY  GPPVP + PK+L FVDT EM RGP++ PGYWVV+GA+L VE G+IS+K 
Sbjct: 527 DVNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKV 586

Query: 606 KYSLLT-ILSEEFL 618
           KYSL T IL +E +
Sbjct: 587 KYSLFTPILGDEVI 600


>AT1G14780.1 | Symbols:  | MAC/Perforin domain-containing protein |
           chr1:5091020-5093873 FORWARD LENGTH=627
          Length = 627

 Score =  417 bits (1073), Expect = e-116,   Method: Compositional matrix adjust.
 Identities = 235/614 (38%), Positives = 358/614 (58%), Gaps = 32/614 (5%)

Query: 24  EKAVSVIGQGYDLCTDIRFSSCKS--------RLVEIDGNRTRDLVLPAGVVVPDVPSSI 75
           E AV  +G+G+DL  D R   CK         RLV +D  + R+L +P   V  +V + I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 76  KCDKGERTRFRSDVLPFNQMSEHFNQQLSLSGKIPSGQFNKMFDMRKG-WSRDAASTKNL 134
            CDKGERTRFRSD+L FN+MSE+FNQ+ S++GKIPSG FN  F  + G W+ DAA+ K+L
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 135 AYDGWFITLYTVELDR-TKITLSENVKKEVPSSWNPAALTEFIEKYGTHIXXXXXXXXXX 193
             D   +TL+ + +    ++ L++ V+  VPSSW+P  L  FIE+YGTH+          
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 194 XXHIKQSKSSDLQPTELQKLLKQLADEGFSEDSNRSSI-VNPIETSGKRKDGHAKLLGQY 252
              ++Q KSSDL    L+  L  L D+ F+     S+  +N        +    +    +
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVF 249

Query: 253 QYKPSLVGRPVVRSHSKNDDITSISVRRGGIDTCQSYNQWLSTISESPNVISMSFVPITS 312
             K ++       S +  + IT I  +RGG    +S+++WL T+ + P+ I+ +F+PITS
Sbjct: 250 DDKQTVAFNNF--SINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITS 307

Query: 313 LLNSVPGNGFLSHAMNLYLRYKPAIEELHQFLEFQLPRQWAPMYDHLPLGFGHKFKRNMS 372
           LL  VPG+G LSHAM+LYLRYKP + +L  FL+F  PR WAP+++ LP G       +  
Sbjct: 308 LLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMA-SAY 366

Query: 373 PSLQFTLMGPKLFVNTVKVDSENRPVTGIRLYLEGRRNNHLSIHLQHLSEVPGVL--EIS 430
           P+L    MGPKL+VNT  V SE  PVTG+R +LEG++ N L+IHLQHL      +  +I+
Sbjct: 367 PALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKIT 426

Query: 431 EDHGYDPIDE-PDDRGYYEPVKWSMFSHVYTAPVQYNSSRIDESTE---------IVTKA 480
           ++H +   D+  D+  Y+EP+    FSHV T PV+Y+ + I  ++          IVT A
Sbjct: 427 DEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGA 486

Query: 481 WFEVKVVGMKKVLFLRLGFSTVASAKIRRSEW-DGPSTSSRKSGFFSAL---MSSKKLNK 536
             EVK  G K VL LRL ++ V+   + ++ W  GP  +S+KSG FS++   ++S  ++ 
Sbjct: 487 QLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVHH 546

Query: 537 EVQSPEGPTKVDVNSAIYHDGPPVPRKTPKMLSFVDTQEMVRGPEDPPGYWVVTGAKLCV 596
            +   +   +V ++S ++  GPPVP    K++ FVD  ++ RGP+  PG+W+VTG +L +
Sbjct: 547 NMIQKD-KNEVVLDSGVFPGGPPVPANN-KIVKFVDLSQLCRGPQHSPGHWLVTGVRLYL 604

Query: 597 EGGRISIKAKYSLL 610
           + G++ +  K++LL
Sbjct: 605 DKGKLCLHVKFALL 618


>AT1G29690.1 | Symbols: CAD1 | MAC/Perforin domain-containing
           protein | chr1:10379310-10381861 REVERSE LENGTH=561
          Length = 561

 Score =  391 bits (1004), Expect = e-109,   Method: Compositional matrix adjust.
 Identities = 229/598 (38%), Positives = 328/598 (54%), Gaps = 75/598 (12%)

Query: 26  AVSVIGQGYDLCTDIRFSSCK----SRLVEIDGNRTRDLVLPAGVVVPDVPSSIKCDKGE 81
           A+  +G+G+D+ +D+R   CK    SRLV I+  + RDL L  G ++P+VP+ I C +G 
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRGN 83

Query: 82  RTRFRSDVLPFNQMSEHFNQQLSLSGKIPSGQFNKMFDMRKGWSRDAASTKNLAYDGWFI 141
               R  V  F++M+E FN +  + G IP G FN MF+    W  DAASTK+LA  G+FI
Sbjct: 84  SGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYFI 143

Query: 142 TLYTVELDRTKITLSENVKKEVPSSWNPAALTEFIEKYGTHIXXXXXXXXXXXXHIKQSK 201
            LY V+L +  + L   +++ VPSSW+PA+L  FIE YGTHI            +I+Q +
Sbjct: 144 PLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQHQ 203

Query: 202 SSDLQPTELQKLLKQLADEGFSEDSNRSSIVNPIETSGKRKDGHAKLLGQYQYKPSLVGR 261
           SS L  +E++  +  +    F E  ++S                  + G  +YK      
Sbjct: 204 SSPLPVSEIENYVNDMIKHRFHEAESQS------------------ITGPLKYK------ 239

Query: 262 PVVRSHSKNDDITSISVRRGGIDTCQSYNQWLSTISESPNVISMSFVPITSLLNSVPGNG 321
                   + DIT I  RRGG D  QS+ +W  T+  +P++I+M+F PI SLL  VPG  
Sbjct: 240 --------DKDITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLR 291

Query: 322 FLSHAMNLYLRYKPAIEELHQFLEFQLPRQWAPMYDHLPLGFGHKFKRNMSPSLQFTLMG 381
            L+ A+ LYL YKP IE+L  FL++Q+ R WAP   +L      + K  +  SLQF+LMG
Sbjct: 292 HLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL------QRKEPVCSSLQFSLMG 345

Query: 382 PKLFVNTVKVDSENRPVTGIRLYLEGRRNNHLSIHLQHLSEVPGVLEISEDHGYDPIDEP 441
           PKLF++  +V    +PVTG+RL LEG + N LSIHLQHL  +P +L+   D  + PI  P
Sbjct: 346 PKLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWD-SHVPIGAP 404

Query: 442 DDRG-------YYEPVKWSMFSHVYTAPVQYNSSRIDE--STEIVTKAWFEVKVVGMKKV 492
             +G       ++EP+KW  FSHV T+P+++  + I +     IVT A   V   G K V
Sbjct: 405 KWQGPEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNV 464

Query: 493 LFLRLGFSTVASAKIRRSEWDGPSTSSRKSGFFSALMSSKKLNKEVQSPEGPTKVDVNSA 552
           L L+L FS V    IRRS WD            + + SS +L      P GP+       
Sbjct: 465 LHLKLLFSKVPGCTIRRSVWD-----------HTPVASSGRLE-----PGGPSTSSSTEE 508

Query: 553 IYHDGPPVPRKTPKMLSFVDTQEMVRGPEDPPGYWVVTGAKLCVEGGRISIKAKYSLL 610
           +         ++ K+   VD+ EM++GP+D PG+W+VTGAKL VE G+I ++ KYSLL
Sbjct: 509 VSG-------QSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559


>AT4G24290.1 | Symbols:  | MAC/Perforin domain-containing protein |
           chr4:12594856-12596590 FORWARD LENGTH=350
          Length = 350

 Score =  313 bits (801), Expect = 3e-85,   Method: Compositional matrix adjust.
 Identities = 167/342 (48%), Positives = 223/342 (65%), Gaps = 16/342 (4%)

Query: 14  GLELDPQLAAEKAVSVIGQGYDLCTDIRFSSCK-----SRLVEI-DGNRTRDLVLPAGVV 67
            L L    AAE A+  IG GYDL  D+R   CK     SRL++I +G+   ++VLP G+ 
Sbjct: 2   ALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGIS 61

Query: 68  VPDVPSSIKCDKGERTRFRSDVLPFNQMSEHFNQQLSLSGKIPSGQFNKMFDMRKGWSRD 127
           +P+V  SIKCDKGER RFRSD+LPF QM+E FNQ+LSL+GKIPSG FN MF+    W +D
Sbjct: 62  IPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKD 121

Query: 128 AASTKNLAYDGWFITLYTVELDRTKITLSENVKKEVPSSWNPAALTEFIEKYGTHIXXXX 187
           AA TKNLA+DG FI+LY+V LD++++ L E+VK+ VPS+W+PAAL  FI+ YGTHI    
Sbjct: 122 AAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSV 181

Query: 188 XXXXXXXXHIKQSKSSDLQPTELQKLLKQLADEGFSEDSNRSSIVNPIETSGKRKDGHAK 247
                   + KQ  SS LQP +LQK LK++AD+ F E    +S+V+   T  +R    +K
Sbjct: 182 KMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVE----ASVVH--NTGSERVQASSK 235

Query: 248 LLGQYQYKPSLVGRPVVRSHSKNDDITSISVRRGGIDTCQ-SYNQWLSTISESPNVISMS 306
           +  + Q +        + S++  +D   +  RRGG D     +N+WL T+   P+VISMS
Sbjct: 236 VETKEQ-RLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMS 294

Query: 307 FVPITSLLNSVPGNGFLSHAMNLYLRYKPA--IEELHQFLEF 346
           F+PITSLLN VPG+GFLSHA+NLYLR KP   +  +H FLE 
Sbjct: 295 FIPITSLLNGVPGSGFLSHAINLYLRCKPINHLRFVHFFLEL 336