Miyakogusa Predicted Gene

Lj3g3v3396900.1
Show Alignment:  
BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v3396900.1 Non Chatacterized Hit- tr|I1LJ56|I1LJ56_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.57517
PE,54.55,1e-18,S5A_REDUCTASE,3-oxo-5-alpha-steroid 4-dehydrogenase,
C-terminal; Steroid_dh,3-oxo-5-alpha-steroid 4-,CUFF.45792.1
         (333 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G16530.1 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase ...   387   e-108
AT2G16530.2 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase ...   380   e-106
AT1G72590.1 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase ...   340   8e-94
AT2G38050.1 | Symbols: DET2, DWF6, ATDET2 | 3-oxo-5-alpha-steroi...    69   6e-12
AT5G16010.1 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase ...    64   1e-10
AT3G43840.1 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase ...    64   2e-10

>AT2G16530.1 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase
           family protein | chr2:7163276-7165064 REVERSE LENGTH=343
          Length = 343

 Score =  387 bits (995), Expect = e-108,   Method: Compositional matrix adjust.
 Identities = 193/341 (56%), Positives = 238/341 (69%), Gaps = 9/341 (2%)

Query: 1   MEVLLVQLLRLAWIA----ATLPIIIASIPTPKLNWLRRTLLGFARRGKIMHSSSQKFTI 56
           +E+ +V L+R AWI     + LP++IASIPT KLN  R  +L FA RGKI+H SSQKFTI
Sbjct: 2   VELEIVWLVRGAWITVWIVSILPLVIASIPTSKLNSFRELVLSFAGRGKILHPSSQKFTI 61

Query: 57  PQRFFLHFYAVASIWTTFLLAMTWVYAYRMVPNVSESFSYSTITSYLTGGSSF----RTG 112
           PQ+ F HFY +  +WTT LLA TW+YA +M P  SE F  S I S L GGS      ++ 
Sbjct: 62  PQKCFAHFYVIGVVWTTLLLAATWMYACKMAPLSSEEFQLSDIASRLAGGSDVFSVHKSN 121

Query: 113 SDKLREIYAAWEAVFLLLLMEVQVLRRLYESIYVFNYSPSARMHILGYLTGMFFYIAAPL 172
              +   +  W AVFLLLLME+ VLRRL ES YVF YSPSARMHILGY  G+FFY+ APL
Sbjct: 122 MTPVEHRFKVWRAVFLLLLMEIHVLRRLIESFYVFKYSPSARMHILGYFAGLFFYVTAPL 181

Query: 173 SLCGDCAIEAFGLLENLLTEFIVKGKNQMQGTELEFFQVLSPLIKLGWKHWIGLAVFSWG 232
           SLC + A E  G + N + EFI  GK+     E      +SPL+KLG   WIG A+F WG
Sbjct: 182 SLCSNIAPEVAGFVGNQVAEFIANGKSHTSAPEFNLLSSISPLMKLGSLQWIGGAIFLWG 241

Query: 233 WIHQYRCHRILGSLRDS-RQVDEYVIPRGDWFEIVSSPHYLSEIVIYASFVVATGGTNLT 291
           WIHQ RCH ILGSLR++  Q  EY+IP GDWF +VSSPH+L+EIV+YA  ++A+GGT++T
Sbjct: 242 WIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDIT 301

Query: 292 IWLLFVFVVANLTFAAVETHNWYHQKFEDYPNSRLAIIPFV 332
           IWLLF FV ANLT+AA ETH WY +KFE+YP +R AI P+V
Sbjct: 302 IWLLFGFVAANLTYAAGETHRWYLRKFENYPANRHAIFPYV 342


>AT2G16530.2 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase
           family protein | chr2:7163276-7165064 REVERSE LENGTH=342
          Length = 342

 Score =  380 bits (977), Expect = e-106,   Method: Compositional matrix adjust.
 Identities = 192/341 (56%), Positives = 237/341 (69%), Gaps = 10/341 (2%)

Query: 1   MEVLLVQLLRLAWIA----ATLPIIIASIPTPKLNWLRRTLLGFARRGKIMHSSSQKFTI 56
           +E+ +V L+R AWI     + LP++IASIPT KLN  R  +L FA RGKI+H SSQ FTI
Sbjct: 2   VELEIVWLVRGAWITVWIVSILPLVIASIPTSKLNSFRELVLSFAGRGKILHPSSQ-FTI 60

Query: 57  PQRFFLHFYAVASIWTTFLLAMTWVYAYRMVPNVSESFSYSTITSYLTGGSSF----RTG 112
           PQ+ F HFY +  +WTT LLA TW+YA +M P  SE F  S I S L GGS      ++ 
Sbjct: 61  PQKCFAHFYVIGVVWTTLLLAATWMYACKMAPLSSEEFQLSDIASRLAGGSDVFSVHKSN 120

Query: 113 SDKLREIYAAWEAVFLLLLMEVQVLRRLYESIYVFNYSPSARMHILGYLTGMFFYIAAPL 172
              +   +  W AVFLLLLME+ VLRRL ES YVF YSPSARMHILGY  G+FFY+ APL
Sbjct: 121 MTPVEHRFKVWRAVFLLLLMEIHVLRRLIESFYVFKYSPSARMHILGYFAGLFFYVTAPL 180

Query: 173 SLCGDCAIEAFGLLENLLTEFIVKGKNQMQGTELEFFQVLSPLIKLGWKHWIGLAVFSWG 232
           SLC + A E  G + N + EFI  GK+     E      +SPL+KLG   WIG A+F WG
Sbjct: 181 SLCSNIAPEVAGFVGNQVAEFIANGKSHTSAPEFNLLSSISPLMKLGSLQWIGGAIFLWG 240

Query: 233 WIHQYRCHRILGSLRDS-RQVDEYVIPRGDWFEIVSSPHYLSEIVIYASFVVATGGTNLT 291
           WIHQ RCH ILGSLR++  Q  EY+IP GDWF +VSSPH+L+EIV+YA  ++A+GGT++T
Sbjct: 241 WIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDIT 300

Query: 292 IWLLFVFVVANLTFAAVETHNWYHQKFEDYPNSRLAIIPFV 332
           IWLLF FV ANLT+AA ETH WY +KFE+YP +R AI P+V
Sbjct: 301 IWLLFGFVAANLTYAAGETHRWYLRKFENYPANRHAIFPYV 341


>AT1G72590.1 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase
           family protein | chr1:27335601-27336989 FORWARD
           LENGTH=320
          Length = 320

 Score =  340 bits (872), Expect = 8e-94,   Method: Compositional matrix adjust.
 Identities = 178/338 (52%), Positives = 224/338 (66%), Gaps = 25/338 (7%)

Query: 1   MEVLLVQLLRLAWIA----ATLPIIIASIPTPKLNWLRRTLLGFARRGKIMHSSSQKFTI 56
           MEV +V L++ AWI     + LP++IASIP+ KLN  R  +L FA RGKI+H SSQKFT+
Sbjct: 1   MEVEIVWLVKAAWITVWIVSILPLVIASIPSSKLNSFRELVLSFAGRGKILHPSSQKFTV 60

Query: 57  PQRFFLHFYAVASIWTTFLLAMTWVYAYRMVPNVSESFSYSTITSYLTGGSS-FRTGSDK 115
           PQ+FF HFY V  +WTT LLA TW+YA +M                  GGS  F      
Sbjct: 61  PQKFFGHFYVVGVVWTTLLLAATWMYACKM-----------------AGGSHVFSFHMTH 103

Query: 116 LREIYAAWEAVFLLLLMEVQVLRRLYESIYVFNYSPSARMHILGYLTGMFFYIAAPLSLC 175
           +   +    AVFLLLLME+ VLRR+ ES YVF YS SARMHIL Y+  +F+Y+AAPLSLC
Sbjct: 104 VEHRFKVGRAVFLLLLMEIHVLRRVIESFYVFKYSTSARMHILAYVGALFYYVAAPLSLC 163

Query: 176 GDCAIEAFGLLENLLTEFIVKGKNQMQGTELEFFQVLSPLIKLGWKHWIGLAVFSWGWIH 235
            + A E    + + + EFI  GK+     +      +SPL+KLG   WIG A+F WGWIH
Sbjct: 164 SNIAPEVARFVGSQVAEFIASGKSHSH--DFNLLLSISPLMKLGSLQWIGGAIFLWGWIH 221

Query: 236 QYRCHRILGSLRD-SRQVDEYVIPRGDWFEIVSSPHYLSEIVIYASFVVATGGTNLTIWL 294
           Q RCH ILGSLR+   Q  EY+IP GDWFE+VS PH+L+EIV+Y   ++++GGT+++IWL
Sbjct: 222 QRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYLGLLISSGGTDISIWL 281

Query: 295 LFVFVVANLTFAAVETHNWYHQKFEDYPNSRLAIIPFV 332
           LF FV ANLT+AA ETH WY QKFE+YP SR AI P V
Sbjct: 282 LFGFVAANLTYAAGETHRWYLQKFENYPASRHAIFPHV 319


>AT2G38050.1 | Symbols: DET2, DWF6, ATDET2 | 3-oxo-5-alpha-steroid
           4-dehydrogenase family protein | chr2:15921303-15922176
           REVERSE LENGTH=262
          Length = 262

 Score = 68.6 bits (166), Expect = 6e-12,   Method: Compositional matrix adjust.
 Identities = 44/117 (37%), Positives = 60/117 (51%), Gaps = 10/117 (8%)

Query: 220 WKHWIGLAVFSWGWIHQYRCHRILGSLRDSRQVDEYVIPRGDWFEIVSSPHYLSEIVIYA 279
           W+  IG+ VF  G        R L  L+   +   YVIPRG WFE+VS P+Y  E + + 
Sbjct: 151 WRFVIGMVVFITGMYINITSDRTLVRLKKENR-GGYVIPRGGWFELVSCPNYFGEAIEWL 209

Query: 280 SFVVAT---GGTNLTIWLLFVFVVANLTFAAVETHNWYHQKF-EDYPNSRLAIIPFV 332
            + V T    G        F++  +NL   A  +H WY  KF E+YP +R A+IPFV
Sbjct: 210 GWAVMTWSWAGIG-----FFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFV 261


>AT5G16010.1 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase
           family protein | chr5:5227982-5229012 FORWARD LENGTH=268
          Length = 268

 Score = 64.3 bits (155), Expect = 1e-10,   Method: Compositional matrix adjust.
 Identities = 38/111 (34%), Positives = 58/111 (52%), Gaps = 8/111 (7%)

Query: 225 GLAVFSWGWIHQYRCHRILGSLRDSRQVDEYVIPRGDWFEIVSSPHYLSEIVIYASFVVA 284
           G+ +F  G +     H +L  LR      EY IP+G  F+I+  PHYL EI+++ SF + 
Sbjct: 162 GVVMFVVGIVGNLYHHVLLAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLI 221

Query: 285 TGGTNLTIWLLFVFVVANLTF---AAVETHNWYHQKFEDYPNSRLAIIPFV 332
           +     TI+  F F +  + +    +  T  WY  KF+D+P    A+IPFV
Sbjct: 222 SQ----TIY-SFSFAMGTMLYLIGRSYATRTWYLSKFDDFPKHIKALIPFV 267


>AT3G43840.1 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase
           family protein | chr3:15700352-15700855 FORWARD
           LENGTH=84
          Length = 84

 Score = 63.5 bits (153), Expect = 2e-10,   Method: Compositional matrix adjust.
 Identities = 30/46 (65%), Positives = 34/46 (73%)

Query: 287 GTNLTIWLLFVFVVANLTFAAVETHNWYHQKFEDYPNSRLAIIPFV 332
             NL IWLLF FV ANLT+AA ETH WY  KFE+YP +R AI P+V
Sbjct: 38  SANLPIWLLFGFVAANLTYAAGETHRWYLAKFENYPANRHAIFPYV 83