Miyakogusa Predicted Gene

Lj3g3v3363560.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v3363560.1 tr|B0BLI5|B0BLI5_LOTJA CM0216.390.nc protein
OS=Lotus japonicus GN=CM0216.390.nc PE=3 SV=1,98.86,0,Thymidylate
synthase/dCMP hydroxymethylase,Thymidylate synthase/dCMP
hydroxymethylase domain; Dihydr,CUFF.45723.1
         (528 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G34570.1 | Symbols: THY-2 | thymidylate synthase 2 | chr4:165...   836   0.0  
AT2G16370.1 | Symbols: THY-1 | thymidylate synthase 1 | chr2:708...   829   0.0  
AT2G21550.1 | Symbols:  | Bifunctional dihydrofolate reductase/t...   586   e-167

>AT4G34570.1 | Symbols: THY-2 | thymidylate synthase 2 |
           chr4:16511129-16514110 REVERSE LENGTH=565
          Length = 565

 Score =  836 bits (2159), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 394/518 (76%), Positives = 451/518 (87%), Gaps = 8/518 (1%)

Query: 14  NGNLELS--PQRTYQAVVIATPDMGISKDGKLPWALPTDQKFFEEITTKTSDPGKKNAVV 71
           NGN+ ++  PQ TYQ VV AT +MGI KDGKLPW LPTD KFF+++T  TSD  KKNAVV
Sbjct: 53  NGNVIMTSKPQSTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDLTLSTSDSAKKNAVV 112

Query: 72  MGRKSWEAIPPEKRPLCGRLNVVLTRSGSFDIATAENVVICGSVSSAMELLASSPYSMSI 131
           MGRK+WE+IP + RPL GRLNVVL+RS  FDIA  ENVV C S+ SA++LLA+ P+S+SI
Sbjct: 113 MGRKTWESIPKKYRPLSGRLNVVLSRSSGFDIANTENVVTCSSIDSALDLLAAPPFSLSI 172

Query: 132 EKVFLTGGGEIFREALNAPGCEAVHITEIEASIECDTFMPRVDFSVFHPWYSSFPLVENN 191
           EKVF+ GGG+I REALN P CEA+HITEI+ SI+CDTF+P VD S + PW SSFP+ EN 
Sbjct: 173 EKVFVIGGGDILREALNKPSCEAIHITEIDTSIDCDTFIPTVDTSAYQPWCSSFPICENG 232

Query: 192 IRYSFNTYVRVRSSAEESLGQNTDPSFDSDSDSLKFEVKKFS-FLPKMIFESHEEYKYLR 250
           +R+SF T+VRV+SS   S G+ +D S    S  L+ + KKFS  LPKMIF+ HEEY YL 
Sbjct: 233 LRFSFTTHVRVKSS---SAGEASDES--DGSKVLQVDWKKFSSVLPKMIFDRHEEYLYLN 287

Query: 251 LVQEIISEGTVKDDRTGTGTLSKFGCQMRFNLRRSFPLLTTKKVFWRGVVEELLWFISGS 310
           LV+EIIS G +KDDRTGTGTLSKFGCQM+FNLRR+FPLLTTK+VFWRGVVEELLWFISGS
Sbjct: 288 LVKEIISNGNLKDDRTGTGTLSKFGCQMKFNLRRNFPLLTTKRVFWRGVVEELLWFISGS 347

Query: 311 TSAKVLQEKGIHIWDGNASREYLDSIGLTEREEGDLGPVYGFQWRHFGARYTNMHDDYSG 370
           T+AKVLQEKGI IWDGNASR YLD IGLTEREEGDLGPVYGFQWRHFGA+YT+MH DY+G
Sbjct: 348 TNAKVLQEKGIRIWDGNASRAYLDGIGLTEREEGDLGPVYGFQWRHFGAKYTDMHADYTG 407

Query: 371 KGFDQLLDVINKIKHNPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQR 430
           +GFDQLLDVINKIK+NPDDRRII+SAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQR
Sbjct: 408 QGFDQLLDVINKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQR 467

Query: 431 SADMGLGVPFNIASYALLTCMIAHVCDLVPGDFIHVIGDAHVYRNHVKPLQEQLQNLPRP 490
           SADMGLGVPFNIASY+LLTC++AHVCDLVPGDFIHVIGDAHVY+NHV+PLQEQL+N P+P
Sbjct: 468 SADMGLGVPFNIASYSLLTCILAHVCDLVPGDFIHVIGDAHVYKNHVRPLQEQLENPPKP 527

Query: 491 FPILKINPKKKDIDSFEATDFKLIGYDPHQKIQMKMAV 528
           FP+LKINP+KKDIDSF A DF+LIGYDPH+KI MKMAV
Sbjct: 528 FPVLKINPEKKDIDSFVADDFELIGYDPHKKIDMKMAV 565


>AT2G16370.1 | Symbols: THY-1 | thymidylate synthase 1 |
           chr2:7082038-7084333 REVERSE LENGTH=519
          Length = 519

 Score =  829 bits (2142), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 390/528 (73%), Positives = 455/528 (86%), Gaps = 9/528 (1%)

Query: 1   MASDSLVISNGHDNGNLELSPQRTYQAVVIATPDMGISKDGKLPWALPTDQKFFEEITTK 60
           MA+ +L   N      L   PQRTYQ VV AT +MGI KDGKLPW LPTD KFF++IT  
Sbjct: 1   MATTTL---NDSVTTTLASEPQRTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDITLT 57

Query: 61  TSDPGKKNAVVMGRKSWEAIPPEKRPLCGRLNVVLTRSGSFDIATAENVVICGSVSSAME 120
           TSD  KKNAVVMGRK+WE+IP + RPL GRLNVVLTRSG FDIA  ENVV C SV SA++
Sbjct: 58  TSDSSKKNAVVMGRKTWESIPIKYRPLSGRLNVVLTRSGGFDIANTENVVTCSSVDSALD 117

Query: 121 LLASSPYSMSIEKVFLTGGGEIFREALNAPGCEAVHITEIEASIECDTFMPRVDFSVFHP 180
           LLA+ PY +SIE+VF+ GGG+I REALN P C+A+H+TEI+ S++CDTF+P +D SV+ P
Sbjct: 118 LLAAPPYCLSIERVFVIGGGDILREALNRPSCDAIHLTEIDTSVDCDTFIPAIDTSVYQP 177

Query: 181 WYSSFPLVENNIRYSFNTYVRVRSSAEESLGQNTDPSFDSDSDSLKFEVKKFSFLPKMIF 240
           W SSFP+ EN +R+ F T+VRV+SSA+ES  ++      + S SL+F+ KKF FLPKM+F
Sbjct: 178 WSSSFPVTENGLRFCFTTFVRVKSSADESSDES------NGSQSLQFDGKKFLFLPKMVF 231

Query: 241 ESHEEYKYLRLVQEIISEGTVKDDRTGTGTLSKFGCQMRFNLRRSFPLLTTKKVFWRGVV 300
           + HEE+ YL +V++IIS G VK+DRTGTGTLSKFGCQM+FNLRRSFPLLTTK+VFWRGVV
Sbjct: 232 DQHEEFLYLNMVEDIISNGNVKNDRTGTGTLSKFGCQMKFNLRRSFPLLTTKRVFWRGVV 291

Query: 301 EELLWFISGSTSAKVLQEKGIHIWDGNASREYLDSIGLTEREEGDLGPVYGFQWRHFGAR 360
           EELLWFISGST+AKVLQEKGIHIWDGNASREYLD IGLTEREEGDLGPVYGFQWRHFGA+
Sbjct: 292 EELLWFISGSTNAKVLQEKGIHIWDGNASREYLDGIGLTEREEGDLGPVYGFQWRHFGAK 351

Query: 361 YTNMHDDYSGKGFDQLLDVINKIKHNPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVAN 420
           YT+MH DY+G+GFDQL+DVI+KIK+NPDDRRII+SAWNPSDLKLMALPPCHMFAQFYVA 
Sbjct: 352 YTDMHADYTGQGFDQLVDVIDKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVAE 411

Query: 421 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLVPGDFIHVIGDAHVYRNHVKPL 480
           GELSCQMYQRSADMGLGVPFNIASY+LLTCM+AHVCDLVPGDFIHV+GDAHVY+ HV+PL
Sbjct: 412 GELSCQMYQRSADMGLGVPFNIASYSLLTCMLAHVCDLVPGDFIHVLGDAHVYKTHVRPL 471

Query: 481 QEQLQNLPRPFPILKINPKKKDIDSFEATDFKLIGYDPHQKIQMKMAV 528
           QEQL NLP+PFP++KINP+KK IDSF A+DF L GYDPH+KI+MKMAV
Sbjct: 472 QEQLLNLPKPFPVMKINPEKKQIDSFVASDFDLTGYDPHKKIEMKMAV 519


>AT2G21550.1 | Symbols:  | Bifunctional dihydrofolate
           reductase/thymidylate synthase | chr2:9227209-9230189
           FORWARD LENGTH=492
          Length = 492

 Score =  586 bits (1510), Expect = e-167,   Method: Compositional matrix adjust.
 Identities = 288/515 (55%), Positives = 379/515 (73%), Gaps = 26/515 (5%)

Query: 1   MASDSLVISNGHDNGNLELSPQRTYQAVVIATPDMGISKDGKLPWALPTDQKFFEEITTK 60
           MAS    I N   +  + ++ +R+YQ V+ AT DMG+  D KLPW LP++ +FF+++TT+
Sbjct: 1   MASTGETICNNGQSA-VSVTRRRSYQVVIAATRDMGLGMDMKLPWDLPSEYQFFQDVTTR 59

Query: 61  TSDPGKKNAVVMGRKSWEAIPPEKRPLCGRLNVVLTRSGSFDIATAENVVICGSVSSAME 120
           TSDP K+NA +MGRKSWE+ P E RPL GRLN+VLT+S   +IA  ENV++  S+ SA+E
Sbjct: 60  TSDPTKRNATIMGRKSWESTPLEIRPLPGRLNIVLTKSSCHNIAIDENVLVSSSMESALE 119

Query: 121 LLASSPYSMSIEKVFLTGGGEIFREALNAPGCEAVHITEIEASIECDTFMPRVDFSVFHP 180
           LLA+ PYS+SIEKVF+ GGGE+ R  +NA  C+A+H+TEI+ S+ CD F PRVD S++ P
Sbjct: 120 LLATEPYSLSIEKVFVIGGGELLRNYMNASICDAIHLTEIDISVPCDAFAPRVDTSLYRP 179

Query: 181 WYSSFPLVENNIRYSFNTYVRVRSSAEESLGQNTDPSFDSDSDSLKFEVKKFSFLPKMIF 240
           WYSSFP+VEN IRYSFNTYVR + +    +G     S  ++SD     +K++SFLPKM+F
Sbjct: 180 WYSSFPVVENGIRYSFNTYVRRKDAI---VGSGEKKSV-AESD-----LKEYSFLPKMVF 230

Query: 241 ESHEEYKYLRLVQEIISEGTVKDDRTGTGTLSKFGCQMRFNLRRSFPLLTTKKVFWRGVV 300
           E HEE+ YL LVQ IIS G + D+     TLSKFGCQMRFNLR++FPLLTTKK+FW GVV
Sbjct: 231 ERHEEFGYLNLVQNIISSGDMNDN----STLSKFGCQMRFNLRKTFPLLTTKKIFWLGVV 286

Query: 301 EELLWFISGSTSAKVLQEKGIHIWDGNASREYLDSIGLTEREE-GDLGPVYGFQWRHFGA 359
           EE+L  ISGS + K   E G HIWD + ++EYLDS G+   EE GD   ++G  W+H  A
Sbjct: 287 EEILQLISGSNNPK---ENGSHIWDTDEAKEYLDSFGVNATEEDGDNPFLHGLHWKHCDA 343

Query: 360 RYTNMHDDYSGKGFDQLLDVINKIKHNPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVA 419
           R+         + F QL DVINKIK+NP D+RI+L+A NP D KL ++ PCH F QFYVA
Sbjct: 344 RFV-------IQEFSQLSDVINKIKNNPHDQRIMLAACNPLDFKL-SVSPCHTFTQFYVA 395

Query: 420 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLVPGDFIHVIGDAHVYRNHVKP 479
           NGE+SCQ+YQ S +  +G+PF+IA+Y+LLTC+IAHVCDL  GDFIHVIG A++ + HVK 
Sbjct: 396 NGEVSCQIYQSSTEASIGIPFSIATYSLLTCIIAHVCDLGAGDFIHVIGQAYINKAHVKA 455

Query: 480 LQEQLQNLPRPFPILKINPKKKDIDSFEATDFKLI 514
           +Q+QLQ  P+PFPILKINP+KK +D+FEA+D +L+
Sbjct: 456 IQKQLQISPKPFPILKINPEKKKMDNFEASDLELM 490