Miyakogusa Predicted Gene
- Lj3g3v2721410.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj3g3v2721410.1 tr|G7LCR6|G7LCR6_MEDTR Acetyl-coenzyme A
synthetase OS=Medicago truncatula GN=MTR_8g035620 PE=4
SV=1,79.82,0,YWTD,NULL; AMP-binding,AMP-dependent synthetase/ligase;
PQQ_2,NULL; LONG-CHAIN-FATTY ACID-COA LIGASE,CUFF.44491.1
(778 letters)
Database: TAIR10_pep
35,386 sequences; 14,482,855 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G35930.1 | Symbols: | AMP-dependent synthetase and ligase fa... 721 0.0
AT3G16170.1 | Symbols: | AMP-dependent synthetase and ligase fa... 64 6e-10
AT1G77240.1 | Symbols: | AMP-dependent synthetase and ligase fa... 59 1e-08
AT1G20560.2 | Symbols: AAE1 | acyl activating enzyme 1 | chr1:71... 57 5e-08
AT1G20560.1 | Symbols: AAE1 | acyl activating enzyme 1 | chr1:71... 57 6e-08
AT1G21540.1 | Symbols: | AMP-dependent synthetase and ligase fa... 54 4e-07
AT1G21530.1 | Symbols: | AMP-dependent synthetase and ligase fa... 54 4e-07
AT1G21530.2 | Symbols: | AMP-dependent synthetase and ligase fa... 54 4e-07
AT4G19010.1 | Symbols: | AMP-dependent synthetase and ligase fa... 51 4e-06
>AT5G35930.1 | Symbols: | AMP-dependent synthetase and ligase family
protein | chr5:14067197-14074404 REVERSE LENGTH=1040
Length = 1040
Score = 721 bits (1862), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 380/774 (49%), Positives = 500/774 (64%), Gaps = 16/774 (2%)
Query: 1 MPLILKEEILTSVPIGLPITDCDVMLVGENDASNEAELYVGGSCISRGYYNESNIMSDEF 60
+P +LK E + SVPIG I++C V+L+G+ D E E+ V G C+S+GY + S+I S+ +
Sbjct: 273 LPRLLKTEEIGSVPIGKSISNCKVVLLGDEDKPYEGEICVSGLCLSQGYMH-SSIESEGY 331
Query: 61 VKLPQSYCCGDSSNAFQGQLYFRTGDLVKQLPSGDFIFLGRKDRIVKVHGQRIALEEVEN 120
VKL + C +N QLY+RTGD +QL SGD IF+GR+DR VK++G+R+ALEE+E
Sbjct: 332 VKLHNNSLCNHLTNDCGSQLYYRTGDYGRQLSSGDLIFIGRRDRTVKLNGKRMALEEIET 391
Query: 121 FLREHPYINDAAVICRNLQAELVLLEAFIVLKDKKRLGELLIPEIRSWMINKLPSAVLPN 180
L +P I +A V+ + EL L+AF+VL + + +I IR+WM KLP ++PN
Sbjct: 392 TLELNPDIAEAVVLLSRDETELASLKAFVVLNKESNSSDGIIFSIRNWMGGKLPPVMIPN 451
Query: 181 RFIFIESFPLSSSGKVNYELLVSSALLTKDVKDKVGHIGCSNLLQLIIKAFHDTLMVEKV 240
F+ +E PL+SSGKV+YE L T +D + G ++LLQ I KA D L+V++V
Sbjct: 452 HFVLVEKLPLTSSGKVDYEALARLKCPTTGAQDMMQSNGTNSLLQNIKKAVCDALLVKEV 511
Query: 241 CNDGDFFMMGGNSISAAHVAHYLGIDLRLLYYNPSPFKLCMALLQKRGSCPLHNSLDHCL 300
+D DFF +GG+S++AAH++H LGID+RL+Y SP +L + L +K G L + H
Sbjct: 512 SDDDDFFAIGGDSLAAAHLSHSLGIDMRLIYQFRSPSRLLIYLSEKEGK--LREDMQHNT 569
Query: 301 QLHTDGQNNHFSSNH-TEKLIPLESRMISEGNQDDLF-----PSKRLKRDSPNVSSSPDV 354
D + + N + +PL S + S L KRLK D S
Sbjct: 570 TQKLDHKIESQNGNGLVSRTVPLHSGVTSGPTPSKLQCEKNNSPKRLKIDYEKFSPK--- 626
Query: 355 TSGDNELFKWYXXXXXXXXXXXRCNKVLYKEKPEVTVKRQTALSDNVPRGCRGHLKNFWK 414
+N+L W RCNKV E + S +PR ++ WK
Sbjct: 627 RMKENKL--WDSGFSQIQCAFSRCNKVHSPESCSNEEANREYWSLEIPRNQMVSMQEIWK 684
Query: 415 VYMESCVDASPVLVFKGPDIYLFIGSHAHKFLCINARSGSIQWEIKLEGRIECTAAIVSD 474
V+MESCVDASP++V K YLFIGSH+ KF CI+A+SGS+ WE LEGRIE +A +V D
Sbjct: 685 VHMESCVDASPLVVLKDSKTYLFIGSHSRKFSCIDAKSGSMYWETILEGRIEGSAMVVGD 744
Query: 475 FSQVVVGCYMGKIYFLDFSNGHICWIFQTSGEVKSQPVVDTRRHLIWCGSHDHNLYALDY 534
FSQVV+GCY GK+YFLDFS G +CW FQ GE+K QPVVDT LIWCGSHDH LYALDY
Sbjct: 745 FSQVVIGCYKGKLYFLDFSTGSLCWKFQACGEIKCQPVVDTSSQLIWCGSHDHTLYALDY 804
Query: 535 KNHCCVYKLPCGGSIYGSPAIDEVRELLYVAFTSGRITAVSISASPFNILWLHELEVPVF 594
++ CCVYKL CGGSI+ SPAIDE LYVA TSGR+ AVSI SPF+ LWL ELE P+F
Sbjct: 805 RSQCCVYKLQCGGSIFASPAIDEGHSSLYVASTSGRVIAVSIKDSPFHTLWLFELEAPIF 864
Query: 595 GSLAVT-QNGSVICCLVDGHVLALDPNGSIVWKKTTDGPIFAGPCIPSSLPHEVLVCSRN 653
GSL +T +VICCLVDG V+A+ P+G+I+W+ T GPIFAGPC+ LP +VLVC RN
Sbjct: 865 GSLCITPSTQNVICCLVDGQVIAMSPSGTIIWRYRTGGPIFAGPCMSHVLPSQVLVCCRN 924
Query: 654 GSVYSFKLEEGDLLWEYNVGAPITASAYVDEHLQLEPDASQSSDRLICICTSSGGIHVLR 713
G VYS + E G L+WE N+G PITASAY+DE+L E +SDRL+ +C+SSG +HVLR
Sbjct: 925 GCVYSLEPESGCLVWEDNIGDPITASAYIDENLHFESHELLASDRLVTVCSSSGRVHVLR 984
Query: 714 VNMNFSEDKEQHTSGVQEFARLNLQGDIFSSPLMIAGHIFVGCRDDYLHCVALE 767
V + ++ H S V E R+ LQ DIFSSP+MI G IFVGCRDDY+HC++LE
Sbjct: 985 VRPSIL-SRDSHDSKVGEITRMELQADIFSSPVMIGGRIFVGCRDDYVHCLSLE 1037
>AT3G16170.1 | Symbols: | AMP-dependent synthetase and ligase
family protein | chr3:5476490-5480128 FORWARD LENGTH=544
Length = 544
Score = 63.5 bits (153), Expect = 6e-10, Method: Compositional matrix adjust.
Identities = 54/199 (27%), Positives = 91/199 (45%), Gaps = 25/199 (12%)
Query: 15 IGLPITDCDVMLV-GENDASNEAELYVGGSCISRGYYNESNIMSDEFVKLPQSYCCGDSS 73
+G P+ + + ENDA+ E+ V + + Y+N LP+ +
Sbjct: 357 VGKPLPGVEAKIKEDENDANGVGEICVKSPSLFKEYWN-----------LPEV-----TK 400
Query: 74 NAFQGQLYFRTGDLVKQLPSGDFIFLGRKDR-IVKVHGQRIALEEVENFLREHPYINDAA 132
+F YF+TGD + G ++ LGR I+KV G +++ E+E+ L EHP + +
Sbjct: 401 ESFTEDGYFKTGDAGRVDEDGYYVILGRNSADIMKVGGYKLSALEIESTLLEHPTVAECC 460
Query: 133 VICRNLQAELVLLEAFIVLKD---KKRLGE----LLIPEIRSWMINKLPSAVLPNRFIFI 185
V+ + A I+ + K+R E + + E+ W +KL LP R +
Sbjct: 461 VLGLTDNDYGEAVTAIIIAESAAKKRREDESKPVITLEELCGWAKDKLAPYKLPTRLLIW 520
Query: 186 ESFPLSSSGKVNYELLVSS 204
ES P ++ GKVN + L S
Sbjct: 521 ESLPRNAMGKVNKKELKKS 539
>AT1G77240.1 | Symbols: | AMP-dependent synthetase and ligase
family protein | chr1:29017958-29019595 REVERSE
LENGTH=545
Length = 545
Score = 59.3 bits (142), Expect = 1e-08, Method: Compositional matrix adjust.
Identities = 48/197 (24%), Positives = 84/197 (42%), Gaps = 22/197 (11%)
Query: 5 LKEEILTSVPIGLPITDCDVMLVGENDASNEAELYVGGSCISRGYYNESNIMSDEFVKLP 64
++ + V + PI+ V ++D + E+ G + GYY +
Sbjct: 363 IRTAVFAEVDVRDPISGKSV----KHDGATVGEIVFRGGSVMLGYYKDPE---------- 408
Query: 65 QSYCCGDSSNAFQGQLYFRTGDLVKQLPSGDFIFLGRKDRIVKVHGQRIALEEVENFLRE 124
++ + + +F TGD+ P G R +V G+ I+ E+E L
Sbjct: 409 ------GTAASMREDGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYT 462
Query: 125 HPYINDAAVICRNLQAELVLLEAFIVLKDKKRLGELLIPEIRSWMINKLPSAVLPNRFIF 184
+P I +AAV+ + + AF+ LK G + EIR + KLP ++P +F
Sbjct: 463 NPAIKEAAVVAKPDKMWGETPCAFVSLKYHD--GSVTEREIREFCKTKLPKYMVPRNVVF 520
Query: 185 IESFPLSSSGKVNYELL 201
+E P +S+GK+ LL
Sbjct: 521 LEELPKTSTGKIQKFLL 537
>AT1G20560.2 | Symbols: AAE1 | acyl activating enzyme 1 |
chr1:7119927-7121363 REVERSE LENGTH=478
Length = 478
Score = 57.0 bits (136), Expect = 5e-08, Method: Compositional matrix adjust.
Identities = 48/164 (29%), Positives = 80/164 (48%), Gaps = 15/164 (9%)
Query: 42 GSCISRGYYNESNIMSDEFVKLPQSYCCGDSSNAFQGQLYFRTGDLVKQLPSGDFIFLGR 101
G + RG N + + ++K P++ + AF+G +F +GDL + P G R
Sbjct: 316 GEVVFRG-----NTVMNGYLKNPEA-----TKEAFKGG-WFWSGDLGVKHPDGYIELKDR 364
Query: 102 KDRIVKVHGQRIALEEVENFLREHPYINDAAVICRNLQAELVLLEAFIVLKDKKRLGELL 161
I+ G+ I+ EVE+ L HP + +AAV+ R + AF+ LKD +
Sbjct: 365 SKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDGSKASA-- 422
Query: 162 IPEIRSWMINKLPSAVLPNRFIFIESFPLSSSGKVNYELLVSSA 205
E+ S+ ++LP + P +F E P +S+GKV +L + A
Sbjct: 423 -EELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKFVLRTKA 464
>AT1G20560.1 | Symbols: AAE1 | acyl activating enzyme 1 |
chr1:7119927-7121730 REVERSE LENGTH=556
Length = 556
Score = 56.6 bits (135), Expect = 6e-08, Method: Compositional matrix adjust.
Identities = 48/164 (29%), Positives = 80/164 (48%), Gaps = 15/164 (9%)
Query: 42 GSCISRGYYNESNIMSDEFVKLPQSYCCGDSSNAFQGQLYFRTGDLVKQLPSGDFIFLGR 101
G + RG N + + ++K P++ + AF+G +F +GDL + P G R
Sbjct: 394 GEVVFRG-----NTVMNGYLKNPEA-----TKEAFKGG-WFWSGDLGVKHPDGYIELKDR 442
Query: 102 KDRIVKVHGQRIALEEVENFLREHPYINDAAVICRNLQAELVLLEAFIVLKDKKRLGELL 161
I+ G+ I+ EVE+ L HP + +AAV+ R + AF+ LKD +
Sbjct: 443 SKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDGSKASA-- 500
Query: 162 IPEIRSWMINKLPSAVLPNRFIFIESFPLSSSGKVNYELLVSSA 205
E+ S+ ++LP + P +F E P +S+GKV +L + A
Sbjct: 501 -EELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKFVLRTKA 542
>AT1G21540.1 | Symbols: | AMP-dependent synthetase and ligase
family protein | chr1:7548758-7550521 REVERSE LENGTH=550
Length = 550
Score = 54.3 bits (129), Expect = 4e-07, Method: Compositional matrix adjust.
Identities = 43/174 (24%), Positives = 75/174 (43%), Gaps = 17/174 (9%)
Query: 29 ENDASNEAELYVGGSCISRGYYNESNIMSDEFVKLPQSYCCGDSSNAFQGQLYFRTGDLV 88
E+D + E+ + G + GYY + ++ + +F +GD+
Sbjct: 385 EHDGVSVGEIVLKGGSVMLGYYKDPE----------------GTAACMREDGWFYSGDVG 428
Query: 89 KQLPSGDFIFLGRKDRIVKVHGQRIALEEVENFLREHPYINDAAVICRNLQAELVLLEAF 148
G R ++ G+ I+ EVE L +P + +AAV+ + + AF
Sbjct: 429 VIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAKPDKMWGETPCAF 488
Query: 149 IVLK-DKKRLGELLIPEIRSWMINKLPSAVLPNRFIFIESFPLSSSGKVNYELL 201
+ LK D G + EIR + +LP ++P + IF E P +S+GK+ LL
Sbjct: 489 VSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTSTGKIQKFLL 542
>AT1G21530.1 | Symbols: | AMP-dependent synthetase and ligase
family protein | chr1:7545151-7546936 REVERSE LENGTH=547
Length = 547
Score = 53.9 bits (128), Expect = 4e-07, Method: Compositional matrix adjust.
Identities = 45/176 (25%), Positives = 78/176 (44%), Gaps = 19/176 (10%)
Query: 29 ENDASNEAELYVGGSCISRGYYNESNIMSDEFVKLPQSYCCGDSSNAFQGQLYFRTGDLV 88
++D + E+ GS + GYY + PQ ++ + +F +GD+
Sbjct: 380 KHDGVSVGEIVFRGSSVMLGYYKD-----------PQG-----TAACMREDGWFYSGDIG 423
Query: 89 KQLPSGDFIFLGRKDRIVKVHGQRIALEEVENFLREHPYINDAAVICRNLQAELVLLEAF 148
G R ++ G+ I+ E+E L +P + +AAV+ + + AF
Sbjct: 424 VIHKDGYLEIKDRSKDVIICGGENISSAEIETVLYTNPVVKEAAVVAKPDKMWGETPCAF 483
Query: 149 IVLK-DKKRLGELLIPE--IRSWMINKLPSAVLPNRFIFIESFPLSSSGKVNYELL 201
+ LK D G + + E IR + KLP ++P + IF E P +S+GK+ LL
Sbjct: 484 VSLKCDNNGDGSVPVTEREIREFCKTKLPKYMVPRKVIFQEELPKTSTGKIQKFLL 539
>AT1G21530.2 | Symbols: | AMP-dependent synthetase and ligase
family protein | chr1:7545151-7546936 REVERSE LENGTH=549
Length = 549
Score = 53.9 bits (128), Expect = 4e-07, Method: Compositional matrix adjust.
Identities = 45/176 (25%), Positives = 78/176 (44%), Gaps = 19/176 (10%)
Query: 29 ENDASNEAELYVGGSCISRGYYNESNIMSDEFVKLPQSYCCGDSSNAFQGQLYFRTGDLV 88
++D + E+ GS + GYY + PQ ++ + +F +GD+
Sbjct: 382 KHDGVSVGEIVFRGSSVMLGYYKD-----------PQG-----TAACMREDGWFYSGDIG 425
Query: 89 KQLPSGDFIFLGRKDRIVKVHGQRIALEEVENFLREHPYINDAAVICRNLQAELVLLEAF 148
G R ++ G+ I+ E+E L +P + +AAV+ + + AF
Sbjct: 426 VIHKDGYLEIKDRSKDVIICGGENISSAEIETVLYTNPVVKEAAVVAKPDKMWGETPCAF 485
Query: 149 IVLK-DKKRLGELLIPE--IRSWMINKLPSAVLPNRFIFIESFPLSSSGKVNYELL 201
+ LK D G + + E IR + KLP ++P + IF E P +S+GK+ LL
Sbjct: 486 VSLKCDNNGDGSVPVTEREIREFCKTKLPKYMVPRKVIFQEELPKTSTGKIQKFLL 541
>AT4G19010.1 | Symbols: | AMP-dependent synthetase and ligase
family protein | chr4:10411715-10414221 REVERSE
LENGTH=566
Length = 566
Score = 50.8 bits (120), Expect = 4e-06, Method: Compositional matrix adjust.
Identities = 42/163 (25%), Positives = 70/163 (42%), Gaps = 19/163 (11%)
Query: 34 NEAELYVGGSCISRGYYNESNIMSDEFVKLPQSYCCGDSSNAFQGQLYFRTGDLVKQLPS 93
N EL++ G + +GY N K Q DS + RTGD+
Sbjct: 406 NRGELWIQGPGVMKGYLNNP--------KATQMSIVEDS--------WLRTGDIAYFDED 449
Query: 94 GDFIFLGRKDRIVKVHGQRIALEEVENFLREHPYINDAAVICRNLQAELVLLEAFIVLKD 153
G + R I+K G +IA ++E L HP I DAAV + + AF+V +
Sbjct: 450 GYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEIPVAFVVRRQ 509
Query: 154 KKRLGELLIPEIRSWMINKLPSAVLPNRFIFIESFPLSSSGKV 196
+ L E ++ S++ +++ + + + S P S +GK+
Sbjct: 510 ETTLSE---EDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKI 549