Miyakogusa Predicted Gene

Lj3g3v0825030.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v0825030.1 tr|Q43445|Q43445_SOYBN
S-adenosyl-L-methionine:delta24-sterol-C-methyltransferase OS=Glycine
max PE=,94.88,0,no description,NULL; Sterol_MT_C,Sterol
methyltransferase C-terminal;
Methyltransf_11,Methyltransfer,CUFF.41605.1
         (333 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G13710.2 | Symbols: SMT1, CPH | sterol methyltransferase 1 | ...   617   e-177
AT5G13710.1 | Symbols: SMT1, CPH | sterol methyltransferase 1 | ...   617   e-177
AT1G20330.1 | Symbols: SMT2, CVP1, FRL1 | sterol methyltransfera...   264   8e-71
AT1G76090.1 | Symbols: SMT3 | sterol methyltransferase 3 | chr1:...   263   1e-70
AT1G48600.1 | Symbols: PMEAMT, AtPMEAMT | S-adenosyl-L-methionin...    74   1e-13
AT1G48600.2 | Symbols: PMEAMT, AtPMEAMT | S-adenosyl-L-methionin...    74   1e-13
AT3G18000.1 | Symbols: NMT1, XPL1, PEAMT | S-adenosyl-L-methioni...    74   2e-13
AT1G73600.1 | Symbols:  | S-adenosyl-L-methionine-dependent meth...    71   1e-12
AT1G73600.2 | Symbols:  | S-adenosyl-L-methionine-dependent meth...    71   1e-12
AT1G64970.1 | Symbols: G-TMT, TMT1, VTE4 | gamma-tocopherol meth...    70   3e-12
AT1G78140.1 | Symbols:  | S-adenosyl-L-methionine-dependent meth...    50   2e-06

>AT5G13710.2 | Symbols: SMT1, CPH | sterol methyltransferase 1 |
           chr5:4424048-4426866 REVERSE LENGTH=336
          Length = 336

 Score =  617 bits (1591), Expect = e-177,   Method: Compositional matrix adjust.
 Identities = 287/333 (86%), Positives = 315/333 (94%)

Query: 1   MDLASNLGGKIDKAEVLSAVEKYEKYHVCYGGQEEERKANYTDMVNKYYDLVTSFYEFGW 60
           MDLASNLGGKIDK++VL+AVEKYE+YHV +GG EEERKANYTDMVNKYYDL TSFYE+GW
Sbjct: 1   MDLASNLGGKIDKSDVLTAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGW 60

Query: 61  GESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGEKVLDVGCGIGGPLREISRFSSTSV 120
           GESFHFA RWKGESLRESIKRHEHFLALQLG++PG+KVLDVGCGIGGPLREI+RFS++ V
Sbjct: 61  GESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVV 120

Query: 121 TGLNNNEYQITRGKELNRIAGVDKTCNFVKADFMKMPFPDNSFDAVYAIEATCHAPDAYG 180
           TGLNNNEYQITRGKELNR+AGVDKTCNFVKADFMKMPFP+NSFDAVYAIEATCHAPDAYG
Sbjct: 121 TGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYG 180

Query: 181 CYKEIFRVLKPGQHFAAYEWCMTDSFDPNNQEHQKIKAEIEIGDGLPDIRLTTKCLEALK 240
           CYKEI+RVLKPGQ FAAYEWCMTD+FDP+N EHQKIK EIEIGDGLPDIRLTTKCLEALK
Sbjct: 181 CYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALK 240

Query: 241 QAGFEVIWEKDLAVDSSLPWYLPLDTSHFSLSSFRLTTVGRLFTKNMVKAMEFVGLAPKG 300
           QAGFEVIWEKDLA DS +PWYLPLD +HFSLSSFRLT VGR  TKNMVK +E++ LAP+G
Sbjct: 241 QAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQG 300

Query: 301 SLRVQDFLEKAADGLVEGGKREIFTPMYFFLAR 333
           S RV +FLE+AA+GLV+GG+REIFTPMYFFLAR
Sbjct: 301 SQRVSNFLEQAAEGLVDGGRREIFTPMYFFLAR 333


>AT5G13710.1 | Symbols: SMT1, CPH | sterol methyltransferase 1 |
           chr5:4424048-4426866 REVERSE LENGTH=336
          Length = 336

 Score =  617 bits (1591), Expect = e-177,   Method: Compositional matrix adjust.
 Identities = 287/333 (86%), Positives = 315/333 (94%)

Query: 1   MDLASNLGGKIDKAEVLSAVEKYEKYHVCYGGQEEERKANYTDMVNKYYDLVTSFYEFGW 60
           MDLASNLGGKIDK++VL+AVEKYE+YHV +GG EEERKANYTDMVNKYYDL TSFYE+GW
Sbjct: 1   MDLASNLGGKIDKSDVLTAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGW 60

Query: 61  GESFHFAPRWKGESLRESIKRHEHFLALQLGLKPGEKVLDVGCGIGGPLREISRFSSTSV 120
           GESFHFA RWKGESLRESIKRHEHFLALQLG++PG+KVLDVGCGIGGPLREI+RFS++ V
Sbjct: 61  GESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVV 120

Query: 121 TGLNNNEYQITRGKELNRIAGVDKTCNFVKADFMKMPFPDNSFDAVYAIEATCHAPDAYG 180
           TGLNNNEYQITRGKELNR+AGVDKTCNFVKADFMKMPFP+NSFDAVYAIEATCHAPDAYG
Sbjct: 121 TGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYG 180

Query: 181 CYKEIFRVLKPGQHFAAYEWCMTDSFDPNNQEHQKIKAEIEIGDGLPDIRLTTKCLEALK 240
           CYKEI+RVLKPGQ FAAYEWCMTD+FDP+N EHQKIK EIEIGDGLPDIRLTTKCLEALK
Sbjct: 181 CYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALK 240

Query: 241 QAGFEVIWEKDLAVDSSLPWYLPLDTSHFSLSSFRLTTVGRLFTKNMVKAMEFVGLAPKG 300
           QAGFEVIWEKDLA DS +PWYLPLD +HFSLSSFRLT VGR  TKNMVK +E++ LAP+G
Sbjct: 241 QAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQG 300

Query: 301 SLRVQDFLEKAADGLVEGGKREIFTPMYFFLAR 333
           S RV +FLE+AA+GLV+GG+REIFTPMYFFLAR
Sbjct: 301 SQRVSNFLEQAAEGLVDGGRREIFTPMYFFLAR 333


>AT1G20330.1 | Symbols: SMT2, CVP1, FRL1 | sterol methyltransferase
           2 | chr1:7038968-7040053 REVERSE LENGTH=361
          Length = 361

 Score =  264 bits (674), Expect = 8e-71,   Method: Compositional matrix adjust.
 Identities = 130/304 (42%), Positives = 185/304 (60%), Gaps = 16/304 (5%)

Query: 33  QEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGL 92
           +E E      D V+ +Y+LVT  YE+GWG+SFHF+P   G+S +++ + HE      + +
Sbjct: 62  KEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQV 121

Query: 93  KPGEKVLDVGCGIGGPLREISRFSSTSVTGLNNNEYQITRGKELNRIAGVDKTCNFVKAD 152
           KPG+K+LDVGCG+GGP+R I+  S  +V G+  NEYQ+ R +  N+ AG+D  C  V  +
Sbjct: 122 KPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCGN 181

Query: 153 FMKMPFPDNSFDAVYAIEATCHAPDAYGCYKEIFRVLKPGQHFAAYEWCMTDSFDPNNQE 212
           F++MPF DNSFD  Y+IEATCHAP     Y EI+RVLKPG  + +YEW  T+ F   + E
Sbjct: 182 FLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDDE 241

Query: 213 HQKIKAEIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAVDSSLPWYLPLDTSHFSLS 272
           H ++   IE GD LP +R      E  K+ GFE++ EKDLA   + PW+  L        
Sbjct: 242 HVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRL-------- 293

Query: 273 SFRLTTVGRL-FTKN--MVKAMEFVGLAPKGSLRVQDFLEKAADGLVEGGKREIFTPMYF 329
                 +GRL + +N  +V+ +  VG+APKG++ V + L K AD L  GG+  IF+PM+ 
Sbjct: 294 -----KMGRLAYWRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGETGIFSPMHM 348

Query: 330 FLAR 333
            L R
Sbjct: 349 ILCR 352


>AT1G76090.1 | Symbols: SMT3 | sterol methyltransferase 3 |
           chr1:28550592-28551671 REVERSE LENGTH=359
          Length = 359

 Score =  263 bits (672), Expect = 1e-70,   Method: Compositional matrix adjust.
 Identities = 134/331 (40%), Positives = 193/331 (58%), Gaps = 13/331 (3%)

Query: 4   ASNLGGKIDKAEVLSAVEKYEKYHVCYGGQEE-ERKANYTDMVNKYYDLVTSFYEFGWGE 62
           AS+L G    AE +   + Y +Y   +   +E E      D V+ +Y+LVT  YE+GWG+
Sbjct: 34  ASDLSGGSISAEKVK--DNYNQYWSFFRKPKEIESAEKVPDFVDTFYNLVTDIYEWGWGQ 91

Query: 63  SFHFAPRWKGESLRESIKRHEHFLALQLGLKPGEKVLDVGCGIGGPLREISRFSSTSVTG 122
           SFHF+P   G+S +++ + HE      + +KPG+K+LD GCG+GGP+R I+  S   VTG
Sbjct: 92  SFHFSPHVPGKSDKDATRIHEEMAVDLIKVKPGQKILDAGCGVGGPMRAIAAHSKAQVTG 151

Query: 123 LNNNEYQITRGKELNRIAGVDKTCNFVKADFMKMPFPDNSFDAVYAIEATCHAPDAYGCY 182
           +  NEYQ+ R K  N+ AG+D  CN V  +F+KMPF +N+FD  Y+IEATCHAP     Y
Sbjct: 152 ITINEYQVQRAKLHNKKAGLDSLCNVVCGNFLKMPFDENTFDGAYSIEATCHAPKLEEVY 211

Query: 183 KEIFRVLKPGQHFAAYEWCMTDSFDPNNQEHQKIKAEIEIGDGLPDIRLTTKCLEALKQA 242
            EIFRV+KPG  F +YEW  T+ +  +++EH+ +   IE GD LP +R         K+ 
Sbjct: 212 SEIFRVMKPGSLFVSYEWVTTEKYRDDDEEHKDVIQGIERGDALPGLRSYADIAVTAKKV 271

Query: 243 GFEVIWEKDLAVDSSLPWYLPLDTSHFSLSSFRLTTVGRLFTKNMVKAMEFVGLAPKGSL 302
           GFEV+ EKDLA   S PW+          +  ++  +       +V  +  +G+APKG++
Sbjct: 272 GFEVVKEKDLAKPPSKPWW----------NRLKMGRIAYWRNHVVVVILSAIGVAPKGTV 321

Query: 303 RVQDFLEKAADGLVEGGKREIFTPMYFFLAR 333
            V   L K AD L  GG+  IF+PM+  L R
Sbjct: 322 DVHKMLFKTADYLTRGGETGIFSPMHMILCR 352


>AT1G48600.1 | Symbols: PMEAMT, AtPMEAMT |
           S-adenosyl-L-methionine-dependent methyltransferases
           superfamily protein | chr1:17966448-17969077 FORWARD
           LENGTH=475
          Length = 475

 Score = 74.3 bits (181), Expect = 1e-13,   Method: Compositional matrix adjust.
 Identities = 53/170 (31%), Positives = 86/170 (50%), Gaps = 12/170 (7%)

Query: 83  EHFLALQLGLKPGEKVLDVGCGIGGPLREISRFSSTSVTGLNNNEYQITRGKELNRIAGV 142
           + F+A ++ LKPG+KVLDVGCGIGG    ++      V G++ +   I+    L R  G+
Sbjct: 256 KEFVA-KMDLKPGQKVLDVGCGIGGGDFYMAENFDVHVVGIDLSVNMISFA--LERAIGL 312

Query: 143 DKTCNFVKADFMKMPFPDNSFDAVYAIEATCHAPDAYGCYKEIFRVLKPGQHFAAYEWCM 202
             +  F  AD     +PDNSFD +Y+ +   H  D    ++  F+ LKPG      ++C 
Sbjct: 313 KCSVEFEVADCTTKTYPDNSFDVIYSRDTILHIQDKPALFRTFFKWLKPGGKVLITDYCR 372

Query: 203 T-DSFDPNNQEHQKIKAEIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKD 251
           + ++  P   E+ K +     G  L D++      + LK AGF+ +  +D
Sbjct: 373 SAETPSPEFAEYIKQR-----GYDLHDVQAYG---QMLKDAGFDDVIAED 414


>AT1G48600.2 | Symbols: PMEAMT, AtPMEAMT |
           S-adenosyl-L-methionine-dependent methyltransferases
           superfamily protein | chr1:17966074-17969077 FORWARD
           LENGTH=491
          Length = 491

 Score = 74.3 bits (181), Expect = 1e-13,   Method: Compositional matrix adjust.
 Identities = 53/170 (31%), Positives = 86/170 (50%), Gaps = 12/170 (7%)

Query: 83  EHFLALQLGLKPGEKVLDVGCGIGGPLREISRFSSTSVTGLNNNEYQITRGKELNRIAGV 142
           + F+A ++ LKPG+KVLDVGCGIGG    ++      V G++ +   I+    L R  G+
Sbjct: 272 KEFVA-KMDLKPGQKVLDVGCGIGGGDFYMAENFDVHVVGIDLSVNMISFA--LERAIGL 328

Query: 143 DKTCNFVKADFMKMPFPDNSFDAVYAIEATCHAPDAYGCYKEIFRVLKPGQHFAAYEWCM 202
             +  F  AD     +PDNSFD +Y+ +   H  D    ++  F+ LKPG      ++C 
Sbjct: 329 KCSVEFEVADCTTKTYPDNSFDVIYSRDTILHIQDKPALFRTFFKWLKPGGKVLITDYCR 388

Query: 203 T-DSFDPNNQEHQKIKAEIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKD 251
           + ++  P   E+ K +     G  L D++      + LK AGF+ +  +D
Sbjct: 389 SAETPSPEFAEYIKQR-----GYDLHDVQAYG---QMLKDAGFDDVIAED 430


>AT3G18000.1 | Symbols: NMT1, XPL1, PEAMT |
           S-adenosyl-L-methionine-dependent methyltransferases
           superfamily protein | chr3:6154578-6157331 FORWARD
           LENGTH=491
          Length = 491

 Score = 73.9 bits (180), Expect = 2e-13,   Method: Compositional matrix adjust.
 Identities = 51/163 (31%), Positives = 80/163 (49%), Gaps = 9/163 (5%)

Query: 89  QLGLKPGEKVLDVGCGIGGPLREISRFSSTSVTGLNNNEYQITRGKELNRIAGVDKTCNF 148
           ++ LKPG+KVLDVGCGIGG    ++      V G++ +   I+    L R  G+  +  F
Sbjct: 277 KMNLKPGQKVLDVGCGIGGGDFYMAEKFDVHVVGIDLSVNMISFA--LERAIGLSCSVEF 334

Query: 149 VKADFMKMPFPDNSFDAVYAIEATCHAPDAYGCYKEIFRVLKPGQHFAAYEWCMTDSFDP 208
             AD     +PDNSFD +Y+ +   H  D    ++  F+ LKPG      ++C +    P
Sbjct: 335 EVADCTTKHYPDNSFDVIYSRDTILHIQDKPALFRTFFKWLKPGGKVLISDYCRSPK-TP 393

Query: 209 NNQEHQKIKAEIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKD 251
           + +  + IK     G  L D++      + LK AGF  +  +D
Sbjct: 394 SAEFSEYIKQR---GYDLHDVQAYG---QMLKDAGFTDVIAED 430


>AT1G73600.1 | Symbols:  | S-adenosyl-L-methionine-dependent
           methyltransferases superfamily protein |
           chr1:27670825-27673400 FORWARD LENGTH=490
          Length = 490

 Score = 71.2 bits (173), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 38/114 (33%), Positives = 59/114 (51%), Gaps = 2/114 (1%)

Query: 90  LGLKPGEKVLDVGCGIGGPLREISRFSSTSVTGLNNNEYQITRGKELNRIAGVDKTCNFV 149
           L LKPG+KVLDVGCGIGG    ++      V G++ +   I+    L    G+  +  F 
Sbjct: 277 LDLKPGQKVLDVGCGIGGGDFYMAENFDVDVVGIDLSVNMISFA--LEHAIGLKCSVEFE 334

Query: 150 KADFMKMPFPDNSFDAVYAIEATCHAPDAYGCYKEIFRVLKPGQHFAAYEWCMT 203
            AD  K  +PDN+FD +Y+ +   H  D    ++  ++ LKPG      ++C +
Sbjct: 335 VADCTKKEYPDNTFDVIYSRDTILHIQDKPALFRRFYKWLKPGGKVLITDYCRS 388


>AT1G73600.2 | Symbols:  | S-adenosyl-L-methionine-dependent
           methyltransferases superfamily protein |
           chr1:27669224-27673400 FORWARD LENGTH=504
          Length = 504

 Score = 71.2 bits (173), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 38/114 (33%), Positives = 59/114 (51%), Gaps = 2/114 (1%)

Query: 90  LGLKPGEKVLDVGCGIGGPLREISRFSSTSVTGLNNNEYQITRGKELNRIAGVDKTCNFV 149
           L LKPG+KVLDVGCGIGG    ++      V G++ +   I+    L    G+  +  F 
Sbjct: 291 LDLKPGQKVLDVGCGIGGGDFYMAENFDVDVVGIDLSVNMISFA--LEHAIGLKCSVEFE 348

Query: 150 KADFMKMPFPDNSFDAVYAIEATCHAPDAYGCYKEIFRVLKPGQHFAAYEWCMT 203
            AD  K  +PDN+FD +Y+ +   H  D    ++  ++ LKPG      ++C +
Sbjct: 349 VADCTKKEYPDNTFDVIYSRDTILHIQDKPALFRRFYKWLKPGGKVLITDYCRS 402


>AT1G64970.1 | Symbols: G-TMT, TMT1, VTE4 | gamma-tocopherol
           methyltransferase | chr1:24134337-24135993 REVERSE
           LENGTH=348
          Length = 348

 Score = 69.7 bits (169), Expect = 3e-12,   Method: Compositional matrix adjust.
 Identities = 35/104 (33%), Positives = 49/104 (47%)

Query: 98  VLDVGCGIGGPLREISRFSSTSVTGLNNNEYQITRGKELNRIAGVDKTCNFVKADFMKMP 157
           V+DVGCGIGG  R ++        G+  +  Q  R  +L     +    +F  AD +  P
Sbjct: 130 VVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLAAAQSLAHKASFQVADALDQP 189

Query: 158 FPDNSFDAVYAIEATCHAPDAYGCYKEIFRVLKPGQHFAAYEWC 201
           F D  FD V+++E+  H PD     KE+ RV  PG       WC
Sbjct: 190 FEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPGGRIIIVTWC 233


>AT1G78140.1 | Symbols:  | S-adenosyl-L-methionine-dependent
           methyltransferases superfamily protein |
           chr1:29401937-29403878 REVERSE LENGTH=355
          Length = 355

 Score = 50.4 bits (119), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 51/158 (32%), Positives = 70/158 (44%), Gaps = 25/158 (15%)

Query: 51  LVTSFYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLGLKP--GEKVLDVGCGIGGP 108
           LV+  YE GW ++F     W G    E     + F   +  LKP  G  ++D  CG G  
Sbjct: 146 LVSFLYERGWRQNFI----WGGFPGPE-----KEFEMAKAYLKPVLGGNIIDASCGSGMF 196

Query: 109 LREISRFSSTS-VTGLNNNEYQITRGKELNRIAGVDKTCNF--------VKADFMKMPFP 159
            R  +R    S V  L+ +E  + +  EL     ++K  NF        V+AD  ++PF 
Sbjct: 197 SRLFTRSDLFSLVIALDYSENMLRQCYEL-----LNKEENFPNKEKLVLVRADIARLPFL 251

Query: 160 DNSFDAVYAIEATCHAPDAYGCYKEIFRVLKPGQHFAA 197
             S DAV+A  A    P       EI RVL+PG  F A
Sbjct: 252 SGSVDAVHAGAALHCWPSPSSAVAEISRVLRPGGVFVA 289