Miyakogusa Predicted Gene

Lj2g3v3281380.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj2g3v3281380.1 Non Chatacterized Hit- tr|I3S745|I3S745_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2
SV=1,99.71,0,EF_HAND_1,EF-Hand 1, calcium-binding site;
2POREKCHANEL,Two pore domain potassium channel; Ion_trans,CUFF.39949.1
         (349 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G55630.2 | Symbols: KCO1, TPK1 | Outward rectifying potassium...   365   e-101
AT5G55630.1 | Symbols: KCO1, TPK1, ATKCO1, ATTPK1 | Outward rect...   365   e-101
AT4G01840.1 | Symbols: KCO5, ATTPK5, ATKCO5, TPK5 | Ca2+ activat...   190   1e-48
AT4G18160.1 | Symbols: KCO6, ATTPK3, ATKCO6, TPK3 | Ca2+ activat...   170   2e-42
AT5G46370.1 | Symbols: KCO2, ATTPK2, ATKCO2 | Ca2+ activated out...   161   6e-40
AT1G02510.1 | Symbols: KCO4, ATKCO4, ATTPK4, TPK4 | Outward rect...   145   4e-35
AT5G46360.1 | Symbols: KCO3, ATKCO3 | Ca2+ activated outward rec...   101   7e-22
AT5G46360.2 | Symbols: KCO3 | Ca2+ activated outward rectifying ...    85   7e-17

>AT5G55630.2 | Symbols: KCO1, TPK1 | Outward rectifying potassium
           channel protein | chr5:22531718-22532893 FORWARD
           LENGTH=363
          Length = 363

 Score =  365 bits (937), Expect = e-101,   Method: Compositional matrix adjust.
 Identities = 177/308 (57%), Positives = 229/308 (74%), Gaps = 5/308 (1%)

Query: 42  LDPPETNGNKLIPLSGSIFGSLHPSFRKVALCLAVYVGVGALAFYLVRNQIKGLKTNRFL 101
           +D P  + +K IP+    F  L+P+ R+V + LA+Y+ +G L FYLVR+QI G KT+  +
Sbjct: 56  IDEPPPHPSK-IPM----FSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVV 110

Query: 102 DALYFTIVTMTTVGYGDLVPNSNLTKLLACAFVFSGMAVVGLILSKAADYLVEKQEALLV 161
           DALYF IVTMTTVGYGDLVPNS+ ++LLACAFVFSGM +VG +LS+AADYLVEKQEALLV
Sbjct: 111 DALYFCIVTMTTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLV 170

Query: 162 KAMHMRENFGPSEILKEIETNKTRYKXXXXXXXXXXXXXXXXXXXXSVEKLDFVDAFYCV 221
           +A H+R++FGP++ILKE+ TNK RYK                     VEK+  + AFYCV
Sbjct: 171 RAFHLRQSFGPTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCV 230

Query: 222 CSTITTLGYGDKSFSTQAGRVFAVIWILIGTITVAQFFLYMAELNTESRQKELAKWVLER 281
           CST+TTLGYGDKSF+++AGR+FAV WIL  +I +AQFFLY+AELNTE++Q+ L KWVL R
Sbjct: 231 CSTVTTLGYGDKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTR 290

Query: 282 KITHXXXXXXXXXXXGTVGADEFVIYKLKEMGKISQEDISLFLKEFEELDVDQSGTLSVS 341
           +IT+           G VGA EF++YKLKEMGKI ++DIS  + EFE+LD D+SGTL+ S
Sbjct: 291 RITNNDLEAADLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTS 350

Query: 342 DITLAQSS 349
           DI LAQ++
Sbjct: 351 DIVLAQTT 358


>AT5G55630.1 | Symbols: KCO1, TPK1, ATKCO1, ATTPK1 | Outward
           rectifying potassium channel protein |
           chr5:22531718-22532893 FORWARD LENGTH=363
          Length = 363

 Score =  365 bits (937), Expect = e-101,   Method: Compositional matrix adjust.
 Identities = 177/308 (57%), Positives = 229/308 (74%), Gaps = 5/308 (1%)

Query: 42  LDPPETNGNKLIPLSGSIFGSLHPSFRKVALCLAVYVGVGALAFYLVRNQIKGLKTNRFL 101
           +D P  + +K IP+    F  L+P+ R+V + LA+Y+ +G L FYLVR+QI G KT+  +
Sbjct: 56  IDEPPPHPSK-IPM----FSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVV 110

Query: 102 DALYFTIVTMTTVGYGDLVPNSNLTKLLACAFVFSGMAVVGLILSKAADYLVEKQEALLV 161
           DALYF IVTMTTVGYGDLVPNS+ ++LLACAFVFSGM +VG +LS+AADYLVEKQEALLV
Sbjct: 111 DALYFCIVTMTTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLV 170

Query: 162 KAMHMRENFGPSEILKEIETNKTRYKXXXXXXXXXXXXXXXXXXXXSVEKLDFVDAFYCV 221
           +A H+R++FGP++ILKE+ TNK RYK                     VEK+  + AFYCV
Sbjct: 171 RAFHLRQSFGPTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCV 230

Query: 222 CSTITTLGYGDKSFSTQAGRVFAVIWILIGTITVAQFFLYMAELNTESRQKELAKWVLER 281
           CST+TTLGYGDKSF+++AGR+FAV WIL  +I +AQFFLY+AELNTE++Q+ L KWVL R
Sbjct: 231 CSTVTTLGYGDKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTR 290

Query: 282 KITHXXXXXXXXXXXGTVGADEFVIYKLKEMGKISQEDISLFLKEFEELDVDQSGTLSVS 341
           +IT+           G VGA EF++YKLKEMGKI ++DIS  + EFE+LD D+SGTL+ S
Sbjct: 291 RITNNDLEAADLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTS 350

Query: 342 DITLAQSS 349
           DI LAQ++
Sbjct: 351 DIVLAQTT 358


>AT4G01840.1 | Symbols: KCO5, ATTPK5, ATKCO5, TPK5 | Ca2+ activated
           outward rectifying K+ channel 5 | chr4:793403-794933
           FORWARD LENGTH=408
          Length = 408

 Score =  190 bits (483), Expect = 1e-48,   Method: Compositional matrix adjust.
 Identities = 114/342 (33%), Positives = 180/342 (52%), Gaps = 25/342 (7%)

Query: 18  SAQKARLQLKKRRLIRSRSAPQT----DL--DPPETNGNKLIPLSGSIFGSLHPSFRKVA 71
           S +  R   + R L RSR+AP      DL   PPET   K  P+S SI        R+  
Sbjct: 71  SDETNRFLSQTRPLHRSRTAPAMVIIKDLRTKPPETK--KPSPVSKSII-------RQAI 121

Query: 72  LCLAVYVGVGALAFYLVRNQIKGLKTNRFLDALYFTIVTMTTVGYGDLVPNSNLTKLLAC 131
             L VY+ +G   +   R+   G++T+  +DALYF IVTM T+GYGD+ P +  TK+ A 
Sbjct: 122 FLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPWTKIFAV 181

Query: 132 AFVFSGMAVVGLILSKAADYLVEKQEALLVKAMHMRE--------NFGPSEILKEIETNK 183
            FV  G   + ++LS   +Y+++ QE++++  +  R+         F   + + + E  +
Sbjct: 182 VFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYIIDFEKGR 241

Query: 184 --TRYKXXXXXXXXXXXXXXXXXXXXSVEKLDFVDAFYCVCSTITTLGYGDKSFSTQAGR 241
              R K                     VE+L FVD+ Y    ++TT+GYGD++F T  GR
Sbjct: 242 MRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRAFKTLQGR 301

Query: 242 VFAVIWILIGTITVAQFFLYMAELNTESRQKELAKWVLERKITHXXXXXXXXXXXGTVGA 301
           +FA +W+L+ T+ VA+ FLY+AE   + R ++  K  L R+IT            G +  
Sbjct: 302 LFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTYQHGFISK 361

Query: 302 DEFVIYKLKEMGKISQEDISLFLKEFEELDVDQSGTLSVSDI 343
            E+++ KLKEMGKI+Q+DI   + +FE+LD +Q G +++ D+
Sbjct: 362 SEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPDL 403


>AT4G18160.1 | Symbols: KCO6, ATTPK3, ATKCO6, TPK3 | Ca2+ activated
           outward rectifying K+ channel 6 | chr4:10055696-10057546
           FORWARD LENGTH=436
          Length = 436

 Score =  170 bits (430), Expect = 2e-42,   Method: Compositional matrix adjust.
 Identities = 96/281 (34%), Positives = 153/281 (54%), Gaps = 7/281 (2%)

Query: 68  RKVALCLAVYVGVGALAFYLVRNQIKGLKTNRFLDALYFTIVTMTTVGYGDLVPNSNLTK 127
           R+    L VY+ +G L ++L R+     +T+  +D LYF IVTM T+GYGD+ PNS +TK
Sbjct: 151 RQAFALLVVYLSLGVLIYWLNRDHYVVNQTHPVVDGLYFCIVTMCTIGYGDITPNSVVTK 210

Query: 128 LLACAFVFSGMAVVGLILSKAADYLVEKQEALLVKAMHMRENFGPSEILKEIETNK---- 183
           L +  FV  G   + ++LS    Y+++ QE+ ++ +   R+   P +    I   K    
Sbjct: 211 LFSIMFVLVGFGFIDILLSGMVSYVLDLQESYMLDSAKRRDE--PEKRRSYIIDVKKGRM 268

Query: 184 -TRYKXXXXXXXXXXXXXXXXXXXXSVEKLDFVDAFYCVCSTITTLGYGDKSFSTQAGRV 242
             R K                     +E++ ++D+FY    ++TT+GYGD++F T  GR+
Sbjct: 269 RIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLDSFYLSVMSVTTVGYGDRAFKTLPGRL 328

Query: 243 FAVIWILIGTITVAQFFLYMAELNTESRQKELAKWVLERKITHXXXXXXXXXXXGTVGAD 302
           FA IW+L+ T+ VA+ FLY+AE   + R +E AK VL   ++            G V   
Sbjct: 329 FAAIWLLVSTLAVARAFLYLAEARVDKRNRERAKKVLCETMSVSQFFAADIDNNGCVSKA 388

Query: 303 EFVIYKLKEMGKISQEDISLFLKEFEELDVDQSGTLSVSDI 343
           E+VIYKLKEM KI+ +DI    K+F++LD   +G +++ D+
Sbjct: 389 EYVIYKLKEMEKITDKDILPISKQFDKLDRCSNGKITLLDL 429


>AT5G46370.1 | Symbols: KCO2, ATTPK2, ATKCO2 | Ca2+ activated
           outward rectifying K+ channel 2 | chr5:18809623-18811720
           REVERSE LENGTH=443
          Length = 443

 Score =  161 bits (407), Expect = 6e-40,   Method: Compositional matrix adjust.
 Identities = 98/281 (34%), Positives = 157/281 (55%), Gaps = 8/281 (2%)

Query: 70  VALCLAVYVGVGALAFYLVRNQIKGLKTNRFLDALYFTIVTMTTVGYGDLVPNSNLTKLL 129
           VAL L VY+ +G L ++L R+     +T+  +DALYF IVTM T+GYGD+ P+S +TKL 
Sbjct: 150 VAL-LVVYLSLGVLIYWLNRDSYNVKQTHPVVDALYFCIVTMCTIGYGDITPDSVVTKLF 208

Query: 130 ACAFVFSGMAVVGLILSKAADYLVEKQEA-LLVKAMHMRENFGPSEILKE--IETNK--- 183
           +  FV  G   + ++LS    Y+++ QE  +L  A +   N    + ++   I+  K   
Sbjct: 209 SIFFVLVGFGFMDILLSGMVTYVLDLQENYMLETARNESLNLNDRDKVRSYIIDVKKGRM 268

Query: 184 -TRYKXXXXXXXXXXXXXXXXXXXXSVEKLDFVDAFYCVCSTITTLGYGDKSFSTQAGRV 242
             R K                     VEK+ ++D+FY    ++TT+GYGD++F+T AGR+
Sbjct: 269 RIRLKVGLALGVVVLCLGFGVLIMHFVEKIGWLDSFYFSVMSVTTVGYGDRAFNTLAGRL 328

Query: 243 FAVIWILIGTITVAQFFLYMAELNTESRQKELAKWVLERKITHXXXXXXXXXXXGTVGAD 302
            A +W+L+ T+ VA+  L++AE   + R +E AK VL   ++            G V   
Sbjct: 329 LAAMWLLVSTLAVARAILFLAESRVDKRNRERAKKVLGESMSISQFLDADIDCNGCVSKA 388

Query: 303 EFVIYKLKEMGKISQEDISLFLKEFEELDVDQSGTLSVSDI 343
           EFVIYKLK+M KI+++DI+    +F++LD   SG +++ D+
Sbjct: 389 EFVIYKLKKMDKITEKDINPIGFQFDKLDRTNSGRITLLDL 429


>AT1G02510.1 | Symbols: KCO4, ATKCO4, ATTPK4, TPK4 | Outward
           rectifying potassium channel protein |
           chr1:520687-521626 REVERSE LENGTH=284
          Length = 284

 Score =  145 bits (366), Expect = 4e-35,   Method: Compositional matrix adjust.
 Identities = 83/267 (31%), Positives = 140/267 (52%), Gaps = 23/267 (8%)

Query: 72  LCLAVYVGVGALAFYLVRNQIKGLKTNRFLDALYFTIVTMTTVGYGDLVPNSNLTKLLAC 131
           + L VY+  G   +   R+Q  G +TN F+DA YF+IVT +TVGYGD+VP+++ TK+L  
Sbjct: 40  ILLLVYLTFGVCTYSFFRDQFSGTETNLFVDAFYFSIVTFSTVGYGDIVPSTSTTKILTI 99

Query: 132 AFVFSGMAVVGLILSKAADYLVEKQEALLVKAMHMRENFGPSEILKEIETNKTRYKXXXX 191
             V +G+  +  +L++   +++  QE  ++  ++   N    + + E    + ++K    
Sbjct: 100 VLVSTGVVFLDYLLNRVVSHVLSLQENAILDRINKTRNRAIRDHIAEDGKIRLKWKLCLA 159

Query: 192 XXXXXXXXXXXXXXXXSVEKLDFVDAFYCVCSTITTLGYGDKSFSTQAGRVFAVIWILIG 251
                             E+LD++D+ Y    ++TT+GYGDK+F T  GR FAV W+L+ 
Sbjct: 160 FCAVGLCVGSGALFLHVFERLDWLDSVYLSVISVTTVGYGDKTFKTVEGRGFAVFWLLLS 219

Query: 252 TITVAQFFLYMAELNTESRQKELAKWVLERKITHXXXXXXXXXXXGTVGADEFVIYKLKE 311
           TI +A  FLY+AE+             ++R                     EF+++KL+E
Sbjct: 220 TIAMATLFLYLAEMR------------IDRTTVMKLPP----------SESEFIVFKLRE 257

Query: 312 MGKISQEDISLFLKEFEEL-DVDQSGT 337
            G+IS++DI   ++EFE L +V  SG+
Sbjct: 258 SGRISEDDIKQIVREFENLEEVPSSGS 284


>AT5G46360.1 | Symbols: KCO3, ATKCO3 | Ca2+ activated outward
           rectifying K+ channel 3 | chr5:18806858-18808134 REVERSE
           LENGTH=260
          Length = 260

 Score =  101 bits (252), Expect = 7e-22,   Method: Compositional matrix adjust.
 Identities = 52/140 (37%), Positives = 83/140 (59%)

Query: 209 VEKLDFVDAFYCVCSTITTLGYGDKSFSTQAGRVFAVIWILIGTITVAQFFLYMAELNTE 268
           V K+ ++D+F      +TT+G+GD++F+T  G   A +W+L+ T+ VA+ FL++A+   +
Sbjct: 120 VVKIGWLDSFCFSVMMVTTVGFGDRAFNTWLGTFLAAVWLLVSTLAVARAFLFLADARAD 179

Query: 269 SRQKELAKWVLERKITHXXXXXXXXXXXGTVGADEFVIYKLKEMGKISQEDISLFLKEFE 328
            R +E AK VL   I+            G +   EF IYKLK+M KI+QED      +F+
Sbjct: 180 KRNRERAKKVLGESISISQFFAADIDNDGRLSLAEFAIYKLKQMEKITQEDFIQICNQFD 239

Query: 329 ELDVDQSGTLSVSDITLAQS 348
           +LD  QSG +++ D+T A S
Sbjct: 240 KLDRTQSGRITLVDLTTATS 259


>AT5G46360.2 | Symbols: KCO3 | Ca2+ activated outward rectifying K+
           channel 3 | chr5:18806785-18808134 REVERSE LENGTH=260
          Length = 260

 Score = 85.1 bits (209), Expect = 7e-17,   Method: Compositional matrix adjust.
 Identities = 48/137 (35%), Positives = 75/137 (54%), Gaps = 2/137 (1%)

Query: 209 VEKLDFVDAFYCVCSTITTLGYGDKSFSTQAGRVFAVIWILIGTITVAQFFLYMAELNTE 268
           V K+ ++D+F      +TT+G+GD++F+T  G   A +W+L+ T+ VA+ FL++A+   +
Sbjct: 120 VVKIGWLDSFCFSVMMVTTVGFGDRAFNTWLGTFLAAVWLLVSTLAVARAFLFLADARAD 179

Query: 269 SRQKELAKWVLERKITHXXXXXXXXXXXGTVGADEFVIYKLKEMGKISQEDISLFLKEFE 328
            R +E AK VL   I+            G +   EF IYKLK+M KI+QED         
Sbjct: 180 KRNRERAKKVLGESISISQFFAADIDNDGRLSLAEFAIYKLKQMEKITQEDFIQICNHNT 239

Query: 329 ELDV--DQSGTLSVSDI 343
             ++  D SG  +V DI
Sbjct: 240 HYNMIEDGSGKSAVLDI 256