Miyakogusa Predicted Gene

Lj2g3v1155190.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj2g3v1155190.1 Non Chatacterized Hit- tr|I3S0S3|I3S0S3_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2
SV=1,99.39,0,HOMOCYSTEINE S-METHYLTRANSFERASE,NULL;
METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE
RELATED,CUFF.36299.1
         (328 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G25900.1 | Symbols: HMT-1, ATHMT-1 | Homocysteine S-methyltra...   475   e-134
AT3G25900.3 | Symbols: HMT-1 | Homocysteine S-methyltransferase ...   430   e-121
AT3G25900.2 | Symbols: HMT-1, ATHMT-1 | Homocysteine S-methyltra...   363   e-100
AT3G22740.1 | Symbols: HMT3 | homocysteine S-methyltransferase 3...   357   5e-99
AT3G63250.1 | Symbols: HMT-2, ATHMT-2, HMT2 | homocysteine methy...   352   2e-97
AT3G63250.2 | Symbols: HMT-2, ATHMT-2, HMT2 | homocysteine methy...   297   5e-81

>AT3G25900.1 | Symbols: HMT-1, ATHMT-1 | Homocysteine
           S-methyltransferase family protein |
           chr3:9480964-9482684 FORWARD LENGTH=326
          Length = 326

 Score =  475 bits (1222), Expect = e-134,   Method: Compositional matrix adjust.
 Identities = 228/325 (70%), Positives = 267/325 (82%), Gaps = 1/325 (0%)

Query: 1   MKSDKKPSSLQDLIENAPGGCAVTDGGFATQLEKHGASFTDPLWSAVCLIKDPHLIKKVH 60
           M  +KK + L+DLI+   GGCAV DGGFATQLE HGA+  DPLWSAV LIK+P LIK+VH
Sbjct: 1   MVLEKKSALLEDLIKKC-GGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVH 59

Query: 61  LEYLEAGANILVTSSYQATLPGFLARGLSIEEGEMLLKRSVKLAVEARDSFWNFSKRNPS 120
           +EYLEAGA+I+VTSSYQAT+PGFL+RGLSIEE E LL++SV+LAVEARD FW    +   
Sbjct: 60  MEYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSG 119

Query: 121 NKYRQALVAASIGSYGAYLADGSEYRGLYGPDVTLEKLKDFHRRRLQVLVEAGPDLLAFE 180
           + Y +ALVAASIGSYGAYLADGSEY G YG +V+L+KLKDFHRRRLQVLVEAGPDLLAFE
Sbjct: 120 HSYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFE 179

Query: 181 TIPNKIXXXXXXXXXXXXXXXIPSWICFTSVDGENAPSGESFKDCLEAINKSDKVGAVGI 240
           TIPNK+               IP+WICFTSVDGE APSGESF++CLE +NKS+ + AVGI
Sbjct: 180 TIPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGI 239

Query: 241 NCAPPHFMENLICKFKQLTKKAIIVYPNSGEVWDGRAKKWLPSKCFHDEDFGFHATRWRD 300
           NCAPP F+ENLI KF +LTKKAI+VYPNSGEVWDG+AK+WLPS+CF D++F   AT+WRD
Sbjct: 240 NCAPPQFIENLIRKFAKLTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRD 299

Query: 301 LGAKIIGGCCRTTPSTIQVISNALR 325
           LGAK+IGGCCRTTPSTI  IS  L+
Sbjct: 300 LGAKLIGGCCRTTPSTINAISRDLK 324


>AT3G25900.3 | Symbols: HMT-1 | Homocysteine S-methyltransferase
           family protein | chr3:9480964-9482684 FORWARD LENGTH=306
          Length = 306

 Score =  430 bits (1106), Expect = e-121,   Method: Compositional matrix adjust.
 Identities = 213/325 (65%), Positives = 250/325 (76%), Gaps = 21/325 (6%)

Query: 1   MKSDKKPSSLQDLIENAPGGCAVTDGGFATQLEKHGASFTDPLWSAVCLIKDPHLIKKVH 60
           M  +KK + L+DLI+   GGCAV DGGFATQLE HGA+  DPLWSAV LIK+P LIK+VH
Sbjct: 1   MVLEKKSALLEDLIKKC-GGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVH 59

Query: 61  LEYLEAGANILVTSSYQATLPGFLARGLSIEEGEMLLKRSVKLAVEARDSFWNFSKRNPS 120
           +EYLEAGA+I+VTSSYQAT+PGFL+RGLSIEE E LL++SV+LAVEARD FW    +   
Sbjct: 60  MEYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSG 119

Query: 121 NKYRQALVAASIGSYGAYLADGSEYRGLYGPDVTLEKLKDFHRRRLQVLVEAGPDLLAFE 180
           + Y +ALVAASIGSYGAYLADGSEY G YG +V+L+KLKDFHRRRLQVLVEAGPDLLAFE
Sbjct: 120 HSYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFE 179

Query: 181 TIPNKIXXXXXXXXXXXXXXXIPSWICFTSVDGENAPSGESFKDCLEAINKSDKVGAVGI 240
           TIPNK+               IP+WICFTSVDGE APSGESF++CLE +NKS+ + A   
Sbjct: 180 TIPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYA--- 236

Query: 241 NCAPPHFMENLICKFKQLTKKAIIVYPNSGEVWDGRAKKWLPSKCFHDEDFGFHATRWRD 300
                            LTKKAI+VYPNSGEVWDG+AK+WLPS+CF D++F   AT+WRD
Sbjct: 237 -----------------LTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRD 279

Query: 301 LGAKIIGGCCRTTPSTIQVISNALR 325
           LGAK+IGGCCRTTPSTI  IS  L+
Sbjct: 280 LGAKLIGGCCRTTPSTINAISRDLK 304


>AT3G25900.2 | Symbols: HMT-1, ATHMT-1 | Homocysteine
           S-methyltransferase family protein |
           chr3:9480964-9482416 FORWARD LENGTH=268
          Length = 268

 Score =  363 bits (931), Expect = e-100,   Method: Compositional matrix adjust.
 Identities = 178/259 (68%), Positives = 208/259 (80%), Gaps = 1/259 (0%)

Query: 1   MKSDKKPSSLQDLIENAPGGCAVTDGGFATQLEKHGASFTDPLWSAVCLIKDPHLIKKVH 60
           M  +KK + L+DLI+   GGCAV DGGFATQLE HGA+  DPLWSAV LIK+P LIK+VH
Sbjct: 1   MVLEKKSALLEDLIKKC-GGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVH 59

Query: 61  LEYLEAGANILVTSSYQATLPGFLARGLSIEEGEMLLKRSVKLAVEARDSFWNFSKRNPS 120
           +EYLEAGA+I+VTSSYQAT+PGFL+RGLSIEE E LL++SV+LAVEARD FW    +   
Sbjct: 60  MEYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSG 119

Query: 121 NKYRQALVAASIGSYGAYLADGSEYRGLYGPDVTLEKLKDFHRRRLQVLVEAGPDLLAFE 180
           + Y +ALVAASIGSYGAYLADGSEY G YG +V+L+KLKDFHRRRLQVLVEAGPDLLAFE
Sbjct: 120 HSYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFE 179

Query: 181 TIPNKIXXXXXXXXXXXXXXXIPSWICFTSVDGENAPSGESFKDCLEAINKSDKVGAVGI 240
           TIPNK+               IP+WICFTSVDGE APSGESF++CLE +NKS+ + AVGI
Sbjct: 180 TIPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGI 239

Query: 241 NCAPPHFMENLICKFKQLT 259
           NCAPP F+ENLI KF + T
Sbjct: 240 NCAPPQFIENLIRKFAKAT 258


>AT3G22740.1 | Symbols: HMT3 | homocysteine S-methyltransferase 3 |
           chr3:8033200-8035710 REVERSE LENGTH=347
          Length = 347

 Score =  357 bits (917), Expect = 5e-99,   Method: Compositional matrix adjust.
 Identities = 170/332 (51%), Positives = 230/332 (69%), Gaps = 10/332 (3%)

Query: 4   DKKPSSLQDLIENAPGGCAVTDGGFATQLEKHGASFTDPLWSAVCLIKDPHLIKKVHLEY 63
           ++  S + D +E   GG AV DGGFAT+L++HGA   DPLWSA CLI  PHL+ KVHL+Y
Sbjct: 7   EETSSLMTDFLEKC-GGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDY 65

Query: 64  LEAGANILVTSSYQATLPGFLARGLSIEEGEMLLKRSVKLAVEARDSF--------WNFS 115
           LE+GANI++T+SYQAT+ GF+A+GLS+ E E LL+RSV++  EAR+ F        W+F+
Sbjct: 66  LESGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDFA 125

Query: 116 KRNPSNKYRQALVAASIGSYGAYLADGSEYRGLYGPDVTLEKLKDFHRRRLQVLVEAGPD 175
               +++ R  LVAAS+GSYGAYLADGSEY G+YG  V+ E LKDFHRRR+Q+L ++G D
Sbjct: 126 YAGKASR-RPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGAD 184

Query: 176 LLAFETIPNKIXXXXXXXXXXXXXXXIPSWICFTSVDGENAPSGESFKDCLEAINKSDKV 235
           L+AFETIPNK+               IP+W  FTS DG + P G+S  +C +  +    V
Sbjct: 185 LIAFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNV 244

Query: 236 GAVGINCAPPHFMENLICKFKQLTKKAIIVYPNSGEVWDGRAKKWLPSKCFHDEDFGFHA 295
            A+GINC  P ++  LI   +Q+T+K I+VYPNSGEV+DG  KKW+ S+   +EDF  + 
Sbjct: 245 VAIGINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDFVSYV 304

Query: 296 TRWRDLGAKIIGGCCRTTPSTIQVISNALREE 327
           ++WRD GA + GGCCRTTP+TI+ I+  L +E
Sbjct: 305 SKWRDAGASLFGGCCRTTPNTIRAIAKVLSDE 336


>AT3G63250.1 | Symbols: HMT-2, ATHMT-2, HMT2 | homocysteine
           methyltransferase 2 | chr3:23370575-23372587 REVERSE
           LENGTH=333
          Length = 333

 Score =  352 bits (902), Expect = 2e-97,   Method: Compositional matrix adjust.
 Identities = 171/322 (53%), Positives = 220/322 (68%), Gaps = 6/322 (1%)

Query: 8   SSLQDLIENAPGGCAVTDGGFATQLEKHGASFTDPLWSAVCLIKDPHLIKKVHLEYLEAG 67
           +S++D ++   GG AV DGG AT+ E+HGA   DPLWSA CL+  PHLI  VHL+YLEAG
Sbjct: 7   NSMKDFLKQT-GGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAG 65

Query: 68  ANILVTSSYQATLPGFLARGLSIEEGEMLLKRSVKLAVEARDSFWNFSKRNPSN-----K 122
           A+I+ ++SYQAT+ GF A+G S EE E LLK+SV++A EAR+S+++    + S      K
Sbjct: 66  ADIISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILK 125

Query: 123 YRQALVAASIGSYGAYLADGSEYRGLYGPDVTLEKLKDFHRRRLQVLVEAGPDLLAFETI 182
            R  LVAAS+GSYGAYLADGSEY G+YG  +TLEKLKDFHRRRLQVL E+G DL+AFETI
Sbjct: 126 KRPILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETI 185

Query: 183 PNKIXXXXXXXXXXXXXXXIPSWICFTSVDGENAPSGESFKDCLEAINKSDKVGAVGINC 242
           PNKI               IP W  F S DG N  SG+S K+C+      +KV AVGINC
Sbjct: 186 PNKIEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINC 245

Query: 243 APPHFMENLICKFKQLTKKAIIVYPNSGEVWDGRAKKWLPSKCFHDEDFGFHATRWRDLG 302
            PP F+E L+ + +++T K I+VYPNSGE +D   K+W+ +    DEDF  +  +W D G
Sbjct: 246 TPPRFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAG 305

Query: 303 AKIIGGCCRTTPSTIQVISNAL 324
             ++GGCCRTTP+TI+ I   L
Sbjct: 306 VSLLGGCCRTTPTTIRAIHKRL 327


>AT3G63250.2 | Symbols: HMT-2, ATHMT-2, HMT2 | homocysteine
           methyltransferase 2 | chr3:23370575-23371974 REVERSE
           LENGTH=293
          Length = 293

 Score =  297 bits (761), Expect = 5e-81,   Method: Compositional matrix adjust.
 Identities = 145/275 (52%), Positives = 189/275 (68%), Gaps = 5/275 (1%)

Query: 55  LIKKVHLEYLEAGANILVTSSYQATLPGFLARGLSIEEGEMLLKRSVKLAVEARDSFWNF 114
           L+++VHL+YLEAGA+I+ ++SYQAT+ GF A+G S EE E LLK+SV++A EAR+S+++ 
Sbjct: 13  LMEQVHLDYLEAGADIISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDK 72

Query: 115 SKRNPSN-----KYRQALVAASIGSYGAYLADGSEYRGLYGPDVTLEKLKDFHRRRLQVL 169
              + S      K R  LVAAS+GSYGAYLADGSEY G+YG  +TLEKLKDFHRRRLQVL
Sbjct: 73  CGTSSSMDDKILKKRPILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVL 132

Query: 170 VEAGPDLLAFETIPNKIXXXXXXXXXXXXXXXIPSWICFTSVDGENAPSGESFKDCLEAI 229
            E+G DL+AFETIPNKI               IP W  F S DG N  SG+S K+C+   
Sbjct: 133 AESGADLIAFETIPNKIEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIA 192

Query: 230 NKSDKVGAVGINCAPPHFMENLICKFKQLTKKAIIVYPNSGEVWDGRAKKWLPSKCFHDE 289
              +KV AVGINC PP F+E L+ + +++T K I+VYPNSGE +D   K+W+ +    DE
Sbjct: 193 ENCEKVVAVGINCTPPRFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDE 252

Query: 290 DFGFHATRWRDLGAKIIGGCCRTTPSTIQVISNAL 324
           DF  +  +W D G  ++GGCCRTTP+TI+ I   L
Sbjct: 253 DFVSYVEKWMDAGVSLLGGCCRTTPTTIRAIHKRL 287