Miyakogusa Predicted Gene

Lj2g3v1020000.2
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj2g3v1020000.2 Non Chatacterized Hit- tr|A9NVM4|A9NVM4_PICSI
Putative uncharacterized protein OS=Picea sitchensis
P,29.14,6e-18,seg,NULL; alpha/beta-Hydrolases,NULL; Lipase_3,Lipase,
class 3; coiled-coil,NULL; no description,NUL,CUFF.35997.2
         (570 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G14930.2 | Symbols: SAG101 | senescence-associated gene 101 |...   266   3e-71
AT5G14930.3 | Symbols: SAG101 | senescence-associated gene 101 |...   130   3e-30
AT3G52430.1 | Symbols: PAD4, ATPAD4 | alpha/beta-Hydrolases supe...   127   3e-29
AT3G48090.1 | Symbols: EDS1, ATEDS1 | alpha/beta-Hydrolases supe...    64   3e-10
AT3G48080.1 | Symbols:  | alpha/beta-Hydrolases superfamily prot...    62   1e-09
AT3G48090.2 | Symbols: EDS1, ATEDS1 | alpha/beta-Hydrolases supe...    59   1e-08
AT3G07400.1 | Symbols:  | lipase class 3 family protein | chr3:2...    51   2e-06

>AT5G14930.2 | Symbols: SAG101 | senescence-associated gene 101 |
           chr5:4828754-4830769 FORWARD LENGTH=537
          Length = 537

 Score =  266 bits (680), Expect = 3e-71,   Method: Compositional matrix adjust.
 Identities = 190/574 (33%), Positives = 286/574 (49%), Gaps = 85/574 (14%)

Query: 10  GIELSPLVISSGLLLRSWDVIASRHDNIVS--DEGL---------GLSWKLYKQPGTDVV 58
           G  L  LV++SGLL  SW  I   H+   S  D GL         GL ++++++    +V
Sbjct: 9   GSALGKLVVTSGLLHSSWSKILEIHNPPYSNHDPGLQVSKKKKDSGLEFQIHREEKFTLV 68

Query: 59  ILAF-----EASSDSS---SKLQPDLVPYSDLLLSTKSNPEFSVNNTAVTLFYENHQKLA 110
           + +       +SSDS+    K + +  P+    L +++NP FS++  A  LF      L 
Sbjct: 69  VFSAPPICRSSSSDSTLLHVKDKENPFPF----LCSENNPSFSLHTPAFNLFTSASTSLT 124

Query: 111 QLLKSEINLSNQSNTKLIVTGHGLGGSVASLFTISLLDNVGSGKKRPLCITFGSPLVGDK 170
             LKSE+  + +S   +I+TG  LGGSVASL+T+ LL+ +    KRPLCITFGSPL+GD 
Sbjct: 125 -YLKSELLQTLKSEKPVIITGAALGGSVASLYTLWLLETIEPTLKRPLCITFGSPLIGDA 183

Query: 171 KLQQAISRSAHWNSCFLHVVSHKDPLPRLFIANSYMPFGTFLFCSDKSSTCFENPDSSLE 230
            LQQ +  S   NSCFLHVVS +  +   F    + PFGTFL C D    C E+  +  E
Sbjct: 184 SLQQILENSVR-NSCFLHVVSAQTRIKMDF----FKPFGTFLICFDSGCVCIEDHVAVTE 238

Query: 231 ILVQLGSINAQSQGNQSSEYGNIIENLKPICKDLTTRAEDKFQPDSLLACISLQLQALGL 290
           +L           G   S   +  + L  + + + + A+ +  P+ ++  I  + +   L
Sbjct: 239 LL----------NGVHDSGLVDYSQVLNRLDQSMLSLADSRLIPEDVIKGIEKRAEMKNL 288

Query: 291 TPXXXXXXXXNIDINXXXXXXXXXXXXXIVQRRIIFDPSKKLNQMKGHMAQLEWYKKETK 350
                                         +  ++F   KKLN MK  MA +EWYKK+ K
Sbjct: 289 ------------------------------RFDMMF---KKLNDMKISMAYIEWYKKKCK 315

Query: 351 DEGKGYYDSYKNM--YYQRDHDV-VELHKK--LTIYWEKMVEEAKLKPQREGSAFRTRYL 405
           +   GYYD +K    +  ++ D+ ++ H K  L  +W+ +VEE + +PQ + S  + R+L
Sbjct: 316 EVKIGYYDRFKTQLAFPSKEFDINIKNHHKSELNRFWKSVVEEVERRPQSDASILKRRFL 375

Query: 406 YGGTTYRRMVEPLAISQXXXXXXXXXXXXXRSKHFELLEEWLNEAGDKDKKNLQSTSKKN 465
           + G  YRRM+EPL I++             RS H+ +LE+W        +K  +   K++
Sbjct: 376 FSGNNYRRMIEPLDIAEYYLEGRKEYRTTGRSHHYVMLEKWFGMESILIEK--ERCKKRD 433

Query: 466 VQAILTIDSCFWAHVEEAL-----LSCXXXXXXXXXXXXXXXXXXXXDYIYGVLKNYEVS 520
           +  +LT DSCFWA VE++L     L+                      Y++ ++   EVS
Sbjct: 434 LSDLLTFDSCFWAEVEDSLIVINQLNTTVGMRDDVREVLTRKLVEFEGYVWEIITKREVS 493

Query: 521 PEIFLEQSSFMRWWIEYKGIKGTSYSSPLASFMN 554
           PEIFLE+SSFM+WW EYK IKG + SS L  FMN
Sbjct: 494 PEIFLEESSFMKWWKEYKKIKGFN-SSYLTEFMN 526


>AT5G14930.3 | Symbols: SAG101 | senescence-associated gene 101 |
           chr5:4828754-4830165 FORWARD LENGTH=239
          Length = 239

 Score =  130 bits (327), Expect = 3e-30,   Method: Compositional matrix adjust.
 Identities = 92/241 (38%), Positives = 131/241 (54%), Gaps = 29/241 (12%)

Query: 10  GIELSPLVISSGLLLRSWDVIASRHDNIVS--DEGL---------GLSWKLYKQPGTDVV 58
           G  L  LV++SGLL  SW  I   H+   S  D GL         GL ++++++    +V
Sbjct: 9   GSALGKLVVTSGLLHSSWSKILEIHNPPYSNHDPGLQVSKKKKDSGLEFQIHREEKFTLV 68

Query: 59  I-----LAFEASSDSS---SKLQPDLVPYSDLLLSTKSNPEFSVNNTAVTLFYENHQKLA 110
           +     +   +SSDS+    K + +  P+    L +++NP FS++  A  LF      L 
Sbjct: 69  VFSAPPICRSSSSDSTLLHVKDKENPFPF----LCSENNPSFSLHTPAFNLFTSASTSLT 124

Query: 111 QLLKSEINLSNQSNTKLIVTGHGLGGSVASLFTISLLDNVGSGKKRPLCITFGSPLVGDK 170
            L KSE+  + +S   +I+TG  LGGSVASL+T+ LL+ +    KRPLCITFGSPL+GD 
Sbjct: 125 YL-KSELLQTLKSEKPVIITGAALGGSVASLYTLWLLETIEPTLKRPLCITFGSPLIGDA 183

Query: 171 KLQQAISRSAHWNSCFLHVVSHKDPLPRLFIANSYMPFGTFLFCSDKSSTCFENPDSSLE 230
            LQQ +  S   NSCFLHVVS +  +   F    + PFGTFL C D    C E+  +  E
Sbjct: 184 SLQQILENSVR-NSCFLHVVSAQTRIKMDF----FKPFGTFLICFDSGCVCIEDHVAVTE 238

Query: 231 I 231
           +
Sbjct: 239 V 239


>AT3G52430.1 | Symbols: PAD4, ATPAD4 | alpha/beta-Hydrolases
           superfamily protein | chr3:19431566-19434292 FORWARD
           LENGTH=541
          Length = 541

 Score =  127 bits (318), Expect = 3e-29,   Method: Compositional matrix adjust.
 Identities = 98/368 (26%), Positives = 167/368 (45%), Gaps = 32/368 (8%)

Query: 127 LIVTGHGLGGSVASLFTISLLDNVGSGKKRPLCITFGSPLVGDKKLQQAISRS--AHWNS 184
           +++TGH  GG++A+   + LL        R  CITFGSPL+G++ L  +ISRS  AH   
Sbjct: 112 VVITGHSTGGALAAFTALWLLSQSSPPSFRVFCITFGSPLLGNQSLSTSISRSRLAH--- 168

Query: 185 CFLHVVSHKDPLPRLFIANSYMPFGTFLFCSDKSSTCFENPDSSLEILVQLGSINAQSQG 244
            F HVVS  D +PR      + PFGT+LFCSDK   C +N   S+ ++  + +  A    
Sbjct: 169 NFCHVVSIHDLVPRS-SNEQFWPFGTYLFCSDKGGVCLDNA-GSVRLMFNILNTTATQNT 226

Query: 245 NQSSEYGNIIENLKPICKDLTTRAEDKFQPDSLLACISLQLQALGLTPXXXXXXXXNIDI 304
            +   YG+ +  L  +     +        +S  A ++L ++ALG +         N D 
Sbjct: 227 EEHQRYGHYVFTLSHMFLKSRSFLGGSIPDNSYQAGVALAVEALGFS---------NDDT 277

Query: 305 NXXXXXXXXXXXXXIVQRRIIFDPSKKLNQMKGHMA---QLEWYKK--ETKDEGKGYYDS 359
           +             IV R  I   ++  N++   +    +++WYK   +  +E  GYYD 
Sbjct: 278 SGVLVKECIETATRIV-RAPILRSAELANELASVLPARLEIQWYKDRCDASEEQLGYYDF 336

Query: 360 YKNMYYQRDHDVVELHKKLTIYWEKMVEEAKLKPQREGSAFRTRYLYGGTTYRRMVEPLA 419
           +K    +RD  V     +L  +W+ +++  +            +++Y    Y+ + EPL 
Sbjct: 337 FKRYSLKRDFKVNMSRIRLAKFWDTVIKMVETNELPFDFHLGKKWIYASQFYQLLAEPLD 396

Query: 420 ISQXXXXXXXXX----XXXXRSKHFELLEEWLNEAGDKDKKNLQSTSKKNVQAILTIDSC 475
           I+                  R K +E++++W  + G K    +     ++  A  T D+C
Sbjct: 397 IANFYKNRDIKTGGHYLEGNRPKRYEVIDKW--QKGVK----VPEECVRSRYASTTQDTC 450

Query: 476 FWAHVEEA 483
           FWA +E+A
Sbjct: 451 FWAKLEQA 458


>AT3G48090.1 | Symbols: EDS1, ATEDS1 | alpha/beta-Hydrolases
           superfamily protein | chr3:17755553-17757692 REVERSE
           LENGTH=623
          Length = 623

 Score = 63.9 bits (154), Expect = 3e-10,   Method: Compositional matrix adjust.
 Identities = 50/198 (25%), Positives = 85/198 (42%), Gaps = 24/198 (12%)

Query: 18  ISSGLLLRSWDVIASRHDNIVSDEGLGLSWKLYKQPGTDVVILAFEASSDSSSKLQPD-L 76
           I+  L+ RSW      +          L+ + +K+    VVI AF+ S        PD  
Sbjct: 9   INGDLITRSWSASKQAY----------LTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNK 58

Query: 77  VPYSDLLLSTKSNP--------EFSVNNTAVTLFYENHQKLAQLLKSEINLSNQSNTKLI 128
             + ++ L+    P        + +  N A     E         ++ + ++ +S  +++
Sbjct: 59  SSFGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIV 118

Query: 129 VTGHGLGGSVASLFTISLLDNVGSGKK----RPLCITFGSPLVGDKKLQQAISRSAHWNS 184
            TGH  GG+ A L T+  L+            P C+TFG+PLVGD     A+ R   W+ 
Sbjct: 119 FTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGRE-KWSR 177

Query: 185 CFLHVVSHKDPLPRLFIA 202
            F++ VS  D +PR+ +A
Sbjct: 178 FFVNFVSRFDIVPRIMLA 195


>AT3G48080.1 | Symbols:  | alpha/beta-Hydrolases superfamily protein
           | chr3:17753104-17755145 REVERSE LENGTH=629
          Length = 629

 Score = 61.6 bits (148), Expect = 1e-09,   Method: Compositional matrix adjust.
 Identities = 45/159 (28%), Positives = 74/159 (46%), Gaps = 14/159 (8%)

Query: 57  VVILAFEASSDSSSKLQPD-LVPYSDLLLSTKSNPEF-SVNNTAVTLFYENHQKLAQLL- 113
            V  AF AS  S      +   P+ ++ +     P   S+ N   T   E   K  ++L 
Sbjct: 38  TVFFAFRASFSSEDLFATENTSPFGEIKMKRNQFPCMRSIGNDVDTTVNEAFLKSLEVLI 97

Query: 114 --KSEINLSNQSNT----KLIVTGHGLGGSVASLFTISLLDNV----GSGKKRPLCITFG 163
             ++  + S QS      +++ TGH  GG+ A L T+  L+            P C+TFG
Sbjct: 98  GPRTSFHASVQSAVDRKQQVVFTGHSFGGATAILATVWYLETYFIRDAYAAPEPRCVTFG 157

Query: 164 SPLVGDKKLQQAISRSAHWNSCFLHVVSHKDPLPRLFIA 202
           +PLVGD   + A+ R  +W+  F++ V+  D +PR+ +A
Sbjct: 158 APLVGDYIFKHALGRE-NWSRFFVNFVTRFDIVPRIMLA 195


>AT3G48090.2 | Symbols: EDS1, ATEDS1 | alpha/beta-Hydrolases
           superfamily protein | chr3:17755553-17757292 REVERSE
           LENGTH=515
          Length = 515

 Score = 58.5 bits (140), Expect = 1e-08,   Method: Compositional matrix adjust.
 Identities = 30/88 (34%), Positives = 48/88 (54%), Gaps = 5/88 (5%)

Query: 119 LSNQSNTKLIVTGHGLGGSVASLFTISLLDNVGSGKK----RPLCITFGSPLVGDKKLQQ 174
           ++ +S  +++ TGH  GG+ A L T+  L+            P C+TFG+PLVGD     
Sbjct: 1   MAVRSRKQIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSH 60

Query: 175 AISRSAHWNSCFLHVVSHKDPLPRLFIA 202
           A+ R   W+  F++ VS  D +PR+ +A
Sbjct: 61  ALGREK-WSRFFVNFVSRFDIVPRIMLA 87


>AT3G07400.1 | Symbols:  | lipase class 3 family protein |
           chr3:2367456-2372238 FORWARD LENGTH=1003
          Length = 1003

 Score = 50.8 bits (120), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 29/89 (32%), Positives = 46/89 (51%), Gaps = 7/89 (7%)

Query: 118 NLSNQSNTKLIVTGHGLGGSVASLFTISLLDNVGSGKKRP------LCITFGSPLVGDKK 171
            L+ +   KL++ GH LGG+VA+L T+++L  V +  KR        CITF  P VG+  
Sbjct: 220 RLAQKKKRKLVLCGHSLGGAVAALATLAILRVVAASSKRGNENIHVKCITFSQPPVGNAA 279

Query: 172 LQQAISRSAHWNSCFLHVVSHKDPLPRLF 200
           L+  +     W+  F      +D +PR+ 
Sbjct: 280 LRDYVHEKG-WHHYFKSYCIPEDLVPRIL 307