Miyakogusa Predicted Gene

Lj1g3v4528440.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v4528440.1 Non Chatacterized Hit- tr|I1JP86|I1JP86_SOYBN
Uncharacterized protein OS=Glycine max PE=3 SV=1,87.89,0,PLP-dependent
transferases,Pyridoxal phosphate-dependent transferase, major domain;
seg,NULL; AROMAT,CUFF.32622.1
         (488 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G20340.1 | Symbols:  | Pyridoxal phosphate (PLP)-dependent tr...   755   0.0  
AT4G28680.1 | Symbols: TYRDC, TYRDC1 | L-tyrosine decarboxylase ...   662   0.0  
AT4G28680.2 | Symbols: TYRDC, TYRDC1 | L-tyrosine decarboxylase ...   659   0.0  
AT4G28680.4 | Symbols: TYRDC | L-tyrosine decarboxylase | chr4:1...   659   0.0  
AT4G28680.3 | Symbols: TYRDC | L-tyrosine decarboxylase | chr4:1...   658   0.0  
AT1G43710.1 | Symbols: emb1075 | Pyridoxal phosphate (PLP)-depen...    55   1e-07

>AT2G20340.1 | Symbols:  | Pyridoxal phosphate (PLP)-dependent
           transferases superfamily protein | chr2:8779804-8782490
           FORWARD LENGTH=490
          Length = 490

 Score =  755 bits (1949), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 349/483 (72%), Positives = 413/483 (85%)

Query: 3   GNGLRPMDAEQLREQGHKMVDFIADYYKTIENYPVLSQVEPGYLGKLLPDSAPTYPESLQ 62
           G  L+PMD+EQLRE GH MVDFIADYYKTIE++PVLSQV+PGYL KLLPDSAP +PE+L 
Sbjct: 6   GKVLKPMDSEQLREYGHLMVDFIADYYKTIEDFPVLSQVQPGYLHKLLPDSAPDHPETLD 65

Query: 63  QVLDDVKEKILPGVTHWQSPNYFAYFPSNSSIAGFLGEMLSAGINIVGFSWITSPAATEL 122
           QVLDDV+ KILPGVTHWQSP++FAY+PSNSS+AGFLGEMLSAG+ IVGFSW+TSPAATEL
Sbjct: 66  QVLDDVRAKILPGVTHWQSPSFFAYYPSNSSVAGFLGEMLSAGLGIVGFSWVTSPAATEL 125

Query: 123 ETIVLDWLAKALNLPDNFYSTGQGGGVIQGTASEXXXXXXXXXRDKILRRVGRDALPKLV 182
           E IVLDW+AK LNLP+ F S G GGGVIQG+ASE         RDK+LR VG++AL KLV
Sbjct: 126 EMIVLDWVAKLLNLPEQFMSKGNGGGVIQGSASEAVLVVLIAARDKVLRSVGKNALEKLV 185

Query: 183 TYASDQTHSALQKACQIAGLNPELCRLLKTDSSTNFALSPDVLSEAISRDIASGLIPFFL 242
            Y+SDQTHSALQKACQIAG++PE CR+L TDSSTN+AL P+ L EA+SRD+ +GLIPFFL
Sbjct: 186 VYSSDQTHSALQKACQIAGIHPENCRVLTTDSSTNYALRPESLQEAVSRDLEAGLIPFFL 245

Query: 243 CATVGTTSSTAVDPLPALAEIAKTNTIWFHVDAAYAGSASICPEYRHHIDGVEEADSFNM 302
           CA VGTTSSTAVDPL AL +IA +N IWFHVDAAYAGSA ICPEYR +IDGVE ADSFNM
Sbjct: 246 CANVGTTSSTAVDPLAALGKIANSNGIWFHVDAAYAGSACICPEYRQYIDGVETADSFNM 305

Query: 303 NAHKWFLTNFDCSLLWVKDRSALIQSLSTNPEFLKNKATQGNLVIDYKDWQIPLGRRFRS 362
           NAHKWFLTNFDCSLLWVKD+ +L  +LSTNPEFLKNKA+Q NLV+DYKDWQIPLGRRFRS
Sbjct: 306 NAHKWFLTNFDCSLLWVKDQDSLTLALSTNPEFLKNKASQANLVVDYKDWQIPLGRRFRS 365

Query: 363 LKLWMVLRLYGLEGLQSHIRSHIAMAAYFEELVGQDTRFKAVAPRTFSLICFRLLPPSNS 422
           LKLWMVLRLYG E L+S+IR+HI +A  FE+LV QD  F+ V PR F+L+CFRL+P  + 
Sbjct: 366 LKLWMVLRLYGSETLKSYIRNHIKLAKEFEQLVSQDPNFEIVTPRIFALVCFRLVPVKDE 425

Query: 423 EDHGNKLNRDLLESVNSTGKIFITHTVLSGEYILRLAVGAPLTEKRHVTMAWQILQDKAT 482
           E   N  NR+LL++VNS+GK+F++HT LSG+ +LR A+GAPLTE++HV  AW+I+Q++A+
Sbjct: 426 EKKCNNRNRELLDAVNSSGKLFMSHTALSGKIVLRCAIGAPLTEEKHVKEAWKIIQEEAS 485

Query: 483 ALL 485
            LL
Sbjct: 486 YLL 488


>AT4G28680.1 | Symbols: TYRDC, TYRDC1 | L-tyrosine decarboxylase |
           chr4:14155248-14158546 FORWARD LENGTH=545
          Length = 545

 Score =  662 bits (1709), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 308/483 (63%), Positives = 379/483 (78%), Gaps = 4/483 (0%)

Query: 6   LRPMDAEQLREQGHKMVDFIADYYKTIEN----YPVLSQVEPGYLGKLLPDSAPTYPESL 61
           ++PMD+E LREQGH MVDFIADYYK +++    +PVLSQV+PGYL  +LPDSAP  PESL
Sbjct: 57  MKPMDSELLREQGHIMVDFIADYYKNLQDSPQDFPVLSQVQPGYLRDMLPDSAPERPESL 116

Query: 62  QQVLDDVKEKILPGVTHWQSPNYFAYFPSNSSIAGFLGEMLSAGINIVGFSWITSPAATE 121
           +++LDDV +KI+PG+THWQSP+YFAY+ S++S+AGFLGEML+AG+++VGF+W+TSPAATE
Sbjct: 117 KELLDDVSKKIMPGITHWQSPSYFAYYASSTSVAGFLGEMLNAGLSVVGFTWLTSPAATE 176

Query: 122 LETIVLDWLAKALNLPDNFYSTGQGGGVIQGTASEXXXXXXXXXRDKILRRVGRDALPKL 181
           LE IVLDWLAK L LPD+F STG GGGVIQGT  E         RD+IL++VG+  LP+L
Sbjct: 177 LEIIVLDWLAKLLQLPDHFLSTGNGGGVIQGTGCEAVLVVVLAARDRILKKVGKTLLPQL 236

Query: 182 VTYASDQTHSALQKACQIAGLNPELCRLLKTDSSTNFALSPDVLSEAISRDIASGLIPFF 241
           V Y SDQTHS+ +KAC I G++ E  RLLKTDSSTN+ + P+ L EAIS D+A G IPFF
Sbjct: 237 VVYGSDQTHSSFRKACLIGGIHEENIRLLKTDSSTNYGMPPESLEEAISHDLAKGFIPFF 296

Query: 242 LCATVGTTSSTAVDPLPALAEIAKTNTIWFHVDAAYAGSASICPEYRHHIDGVEEADSFN 301
           +CATVGTTSS AVDPL  L  IAK   IW HVDAAYAG+A ICPEYR  IDG+E ADSFN
Sbjct: 297 ICATVGTTSSAAVDPLVPLGNIAKKYGIWLHVDAAYAGNACICPEYRKFIDGIENADSFN 356

Query: 302 MNAHKWFLTNFDCSLLWVKDRSALIQSLSTNPEFLKNKATQGNLVIDYKDWQIPLGRRFR 361
           MNAHKW   N  CS LWVKDR +LI +L TNPE+L+ K ++ + V++YKDWQI L RRFR
Sbjct: 357 MNAHKWLFANQTCSPLWVKDRYSLIDALKTNPEYLEFKVSKKDTVVNYKDWQISLSRRFR 416

Query: 362 SLKLWMVLRLYGLEGLQSHIRSHIAMAAYFEELVGQDTRFKAVAPRTFSLICFRLLPPSN 421
           SLKLWMVLRLYG E L++ IR H+ +A +FE+ V QD  F+ V  R FSL+CFRL P   
Sbjct: 417 SLKLWMVLRLYGSENLRNFIRDHVNLAKHFEDYVAQDPSFEVVTTRYFSLVCFRLAPVDG 476

Query: 422 SEDHGNKLNRDLLESVNSTGKIFITHTVLSGEYILRLAVGAPLTEKRHVTMAWQILQDKA 481
            ED  N+ NR+LL +VNSTGKIFI+HT LSG+++LR AVGAPLTE++HVT AWQI+Q  A
Sbjct: 477 DEDQCNERNRELLAAVNSTGKIFISHTALSGKFVLRFAVGAPLTEEKHVTEAWQIIQKHA 536

Query: 482 TAL 484
           +  
Sbjct: 537 SKF 539


>AT4G28680.2 | Symbols: TYRDC, TYRDC1 | L-tyrosine decarboxylase |
           chr4:14155248-14158546 FORWARD LENGTH=547
          Length = 547

 Score =  659 bits (1701), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 309/485 (63%), Positives = 379/485 (78%), Gaps = 6/485 (1%)

Query: 6   LRPMDAEQLREQGHKMVDFIADYYKTIEN----YPVLSQVEPGYLGKLLPDSAPTYPESL 61
           ++PMD+E LREQGH MVDFIADYYK +++    +PVLSQV+PGYL  +LPDSAP  PESL
Sbjct: 57  MKPMDSELLREQGHIMVDFIADYYKNLQDSPQDFPVLSQVQPGYLRDMLPDSAPERPESL 116

Query: 62  QQVLDDVKEKILPGVTHWQSPNYFAYFPSNSSIAGFLGEMLSAGINIVGFSWITSPAATE 121
           +++LDDV +KI+PG+THWQSP+YFAY+ S++S+AGFLGEML+AG+++VGF+W+TSPAATE
Sbjct: 117 KELLDDVSKKIMPGITHWQSPSYFAYYASSTSVAGFLGEMLNAGLSVVGFTWLTSPAATE 176

Query: 122 LETIVLDWLAKALNLPDNFYSTGQGGGVIQGTASEXXXXXXXXXRDKILRRVGRDALPKL 181
           LE IVLDWLAK L LPD+F STG GGGVIQGT  E         RD+IL++VG+  LP+L
Sbjct: 177 LEIIVLDWLAKLLQLPDHFLSTGNGGGVIQGTGCEAVLVVVLAARDRILKKVGKTLLPQL 236

Query: 182 VTYASDQTHSALQKACQIAGLNPELCRLLKTDSSTNFALSPDVLSEAISRDIASGLIPFF 241
           V Y SDQTHS+ +KAC I G++ E  RLLKTDSSTN+ + P+ L EAIS D+A G IPFF
Sbjct: 237 VVYGSDQTHSSFRKACLIGGIHEENIRLLKTDSSTNYGMPPESLEEAISHDLAKGFIPFF 296

Query: 242 LCATVGTTSSTAVDPLPALAEIAKTNTIWFHVDAAYAGSASICPEYRHHIDGVEEADSFN 301
           +CATVGTTSS AVDPL  L  IAK   IW HVDAAYAG+A ICPEYR  IDG+E ADSFN
Sbjct: 297 ICATVGTTSSAAVDPLVPLGNIAKKYGIWLHVDAAYAGNACICPEYRKFIDGIENADSFN 356

Query: 302 MNAHKWFLTNFDCSLLWVKDRSALIQSLSTNPEFL--KNKATQGNLVIDYKDWQIPLGRR 359
           MNAHKW   N  CS LWVKDR +LI +L TNPE+L  K K ++ + V++YKDWQI L RR
Sbjct: 357 MNAHKWLFANQTCSPLWVKDRYSLIDALKTNPEYLEFKVKVSKKDTVVNYKDWQISLSRR 416

Query: 360 FRSLKLWMVLRLYGLEGLQSHIRSHIAMAAYFEELVGQDTRFKAVAPRTFSLICFRLLPP 419
           FRSLKLWMVLRLYG E L++ IR H+ +A +FE+ V QD  F+ V  R FSL+CFRL P 
Sbjct: 417 FRSLKLWMVLRLYGSENLRNFIRDHVNLAKHFEDYVAQDPSFEVVTTRYFSLVCFRLAPV 476

Query: 420 SNSEDHGNKLNRDLLESVNSTGKIFITHTVLSGEYILRLAVGAPLTEKRHVTMAWQILQD 479
              ED  N+ NR+LL +VNSTGKIFI+HT LSG+++LR AVGAPLTE++HVT AWQI+Q 
Sbjct: 477 DGDEDQCNERNRELLAAVNSTGKIFISHTALSGKFVLRFAVGAPLTEEKHVTEAWQIIQK 536

Query: 480 KATAL 484
            A+  
Sbjct: 537 HASKF 541


>AT4G28680.4 | Symbols: TYRDC | L-tyrosine decarboxylase |
           chr4:14155184-14158546 FORWARD LENGTH=538
          Length = 538

 Score =  659 bits (1699), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 308/485 (63%), Positives = 380/485 (78%), Gaps = 6/485 (1%)

Query: 6   LRPMDAEQLREQGHKMVDFIADYYKTIEN----YPVLSQVEPGYLGKLLPDSAPTYPESL 61
           ++PMD+E LREQGH MVDFIADYYK +++    +PVLSQV+PGYL  +LPDSAP  PESL
Sbjct: 48  MKPMDSELLREQGHIMVDFIADYYKNLQDSPQDFPVLSQVQPGYLRDMLPDSAPERPESL 107

Query: 62  QQVLDDVKEKILPGVTHWQSPNYFAYFPSNSSIAGFLGEMLSAGINIVGFSWITSPAATE 121
           +++LDDV +KI+PG+THWQSP+YFAY+ S++S+AGFLGEML+AG+++VGF+W+TSPAATE
Sbjct: 108 KELLDDVSKKIMPGITHWQSPSYFAYYASSTSVAGFLGEMLNAGLSVVGFTWLTSPAATE 167

Query: 122 LETIVLDWLAKALNLPDNFYSTGQG--GGVIQGTASEXXXXXXXXXRDKILRRVGRDALP 179
           LE IVLDWLAK L LPD+F STG+G  GGVIQGT  E         RD+IL++VG+  LP
Sbjct: 168 LEIIVLDWLAKLLQLPDHFLSTGKGNGGGVIQGTGCEAVLVVVLAARDRILKKVGKTLLP 227

Query: 180 KLVTYASDQTHSALQKACQIAGLNPELCRLLKTDSSTNFALSPDVLSEAISRDIASGLIP 239
           +LV Y SDQTHS+ +KAC I G++ E  RLLKTDSSTN+ + P+ L EAIS D+A G IP
Sbjct: 228 QLVVYGSDQTHSSFRKACLIGGIHEENIRLLKTDSSTNYGMPPESLEEAISHDLAKGFIP 287

Query: 240 FFLCATVGTTSSTAVDPLPALAEIAKTNTIWFHVDAAYAGSASICPEYRHHIDGVEEADS 299
           FF+CATVGTTSS AVDPL  L  IAK   IW HVDAAYAG+A ICPEYR  IDG+E ADS
Sbjct: 288 FFICATVGTTSSAAVDPLVPLGNIAKKYGIWLHVDAAYAGNACICPEYRKFIDGIENADS 347

Query: 300 FNMNAHKWFLTNFDCSLLWVKDRSALIQSLSTNPEFLKNKATQGNLVIDYKDWQIPLGRR 359
           FNMNAHKW   N  CS LWVKDR +LI +L TNPE+L+ K ++ + V++YKDWQI L RR
Sbjct: 348 FNMNAHKWLFANQTCSPLWVKDRYSLIDALKTNPEYLEFKVSKKDTVVNYKDWQISLSRR 407

Query: 360 FRSLKLWMVLRLYGLEGLQSHIRSHIAMAAYFEELVGQDTRFKAVAPRTFSLICFRLLPP 419
           FRSLKLWMVLRLYG E L++ IR H+ +A +FE+ V QD  F+ V  R FSL+CFRL P 
Sbjct: 408 FRSLKLWMVLRLYGSENLRNFIRDHVNLAKHFEDYVAQDPSFEVVTTRYFSLVCFRLAPV 467

Query: 420 SNSEDHGNKLNRDLLESVNSTGKIFITHTVLSGEYILRLAVGAPLTEKRHVTMAWQILQD 479
              ED  N+ NR+LL +VNSTGKIFI+HT LSG+++LR AVGAPLTE++HVT AWQI+Q 
Sbjct: 468 DGDEDQCNERNRELLAAVNSTGKIFISHTALSGKFVLRFAVGAPLTEEKHVTEAWQIIQK 527

Query: 480 KATAL 484
            A+  
Sbjct: 528 HASKF 532


>AT4G28680.3 | Symbols: TYRDC | L-tyrosine decarboxylase |
           chr4:14155248-14158546 FORWARD LENGTH=547
          Length = 547

 Score =  658 bits (1698), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 308/485 (63%), Positives = 380/485 (78%), Gaps = 6/485 (1%)

Query: 6   LRPMDAEQLREQGHKMVDFIADYYKTIEN----YPVLSQVEPGYLGKLLPDSAPTYPESL 61
           ++PMD+E LREQGH MVDFIADYYK +++    +PVLSQV+PGYL  +LPDSAP  PESL
Sbjct: 57  MKPMDSELLREQGHIMVDFIADYYKNLQDSPQDFPVLSQVQPGYLRDMLPDSAPERPESL 116

Query: 62  QQVLDDVKEKILPGVTHWQSPNYFAYFPSNSSIAGFLGEMLSAGINIVGFSWITSPAATE 121
           +++LDDV +KI+PG+THWQSP+YFAY+ S++S+AGFLGEML+AG+++VGF+W+TSPAATE
Sbjct: 117 KELLDDVSKKIMPGITHWQSPSYFAYYASSTSVAGFLGEMLNAGLSVVGFTWLTSPAATE 176

Query: 122 LETIVLDWLAKALNLPDNFYSTGQG--GGVIQGTASEXXXXXXXXXRDKILRRVGRDALP 179
           LE IVLDWLAK L LPD+F STG+G  GGVIQGT  E         RD+IL++VG+  LP
Sbjct: 177 LEIIVLDWLAKLLQLPDHFLSTGKGNGGGVIQGTGCEAVLVVVLAARDRILKKVGKTLLP 236

Query: 180 KLVTYASDQTHSALQKACQIAGLNPELCRLLKTDSSTNFALSPDVLSEAISRDIASGLIP 239
           +LV Y SDQTHS+ +KAC I G++ E  RLLKTDSSTN+ + P+ L EAIS D+A G IP
Sbjct: 237 QLVVYGSDQTHSSFRKACLIGGIHEENIRLLKTDSSTNYGMPPESLEEAISHDLAKGFIP 296

Query: 240 FFLCATVGTTSSTAVDPLPALAEIAKTNTIWFHVDAAYAGSASICPEYRHHIDGVEEADS 299
           FF+CATVGTTSS AVDPL  L  IAK   IW HVDAAYAG+A ICPEYR  IDG+E ADS
Sbjct: 297 FFICATVGTTSSAAVDPLVPLGNIAKKYGIWLHVDAAYAGNACICPEYRKFIDGIENADS 356

Query: 300 FNMNAHKWFLTNFDCSLLWVKDRSALIQSLSTNPEFLKNKATQGNLVIDYKDWQIPLGRR 359
           FNMNAHKW   N  CS LWVKDR +LI +L TNPE+L+ K ++ + V++YKDWQI L RR
Sbjct: 357 FNMNAHKWLFANQTCSPLWVKDRYSLIDALKTNPEYLEFKVSKKDTVVNYKDWQISLSRR 416

Query: 360 FRSLKLWMVLRLYGLEGLQSHIRSHIAMAAYFEELVGQDTRFKAVAPRTFSLICFRLLPP 419
           FRSLKLWMVLRLYG E L++ IR H+ +A +FE+ V QD  F+ V  R FSL+CFRL P 
Sbjct: 417 FRSLKLWMVLRLYGSENLRNFIRDHVNLAKHFEDYVAQDPSFEVVTTRYFSLVCFRLAPV 476

Query: 420 SNSEDHGNKLNRDLLESVNSTGKIFITHTVLSGEYILRLAVGAPLTEKRHVTMAWQILQD 479
              ED  N+ NR+LL +VNSTGKIFI+HT LSG+++LR AVGAPLTE++HVT AWQI+Q 
Sbjct: 477 DGDEDQCNERNRELLAAVNSTGKIFISHTALSGKFVLRFAVGAPLTEEKHVTEAWQIIQK 536

Query: 480 KATAL 484
            A+  
Sbjct: 537 HASKF 541


>AT1G43710.1 | Symbols: emb1075 | Pyridoxal phosphate
           (PLP)-dependent transferases superfamily protein |
           chr1:16486534-16488298 REVERSE LENGTH=482
          Length = 482

 Score = 55.1 bits (131), Expect = 1e-07,   Method: Compositional matrix adjust.
 Identities = 73/323 (22%), Positives = 129/323 (39%), Gaps = 44/323 (13%)

Query: 80  QSPNYFAYFPSNSSIA-GFLGEMLSAGINIVGFSWITSPAATE---LETIVLDWLAKALN 135
           ++ N+  Y P N     G LG++    IN +G  +I S         E  VLDW A+   
Sbjct: 102 RTKNHLGY-PYNLDFDYGALGQLQHFSINNLGDPFIESNYGVHSRPFEVGVLDWFARLWE 160

Query: 136 LPDNFYSTGQGGGVIQGTASEXXXXXXXXXRDKILRRVGRDALPKLVTYASDQTHSALQK 195
           +  + Y      G I    +E                VGR+  P  + YAS ++H ++ K
Sbjct: 161 IERDDY-----WGYITNCGTEGNLHGIL---------VGREMFPDGILYASRESHYSVFK 206

Query: 196 ACQIAGLNPELCRLLKTDSSTNFALSPDVLSEAISRDIASGLIPFFLCATVGTTSSTAVD 255
           A ++  +  E     K D+  +  +  D L + +   +A+   P  L   +GTT   AVD
Sbjct: 207 AARMYRMECE-----KVDTLMSGEIDCDDLRKKL---LANKDKPAILNVNIGTTVKGAVD 258

Query: 256 PLPALAEIAKT-----NTIWFHVDAAYAGSASICPEYRHHIDGVEEADSFNMNAHKWFLT 310
            L  + +  +      +  + H D A  G      +    +   +   S +++ HK+   
Sbjct: 259 DLDLVIKTLEECGFSHDRFYIHCDGALFGLMMPFVKRAPKVTFNKPIGSVSVSGHKFVGC 318

Query: 311 NFDCSLLWVKDRSALIQSLSTNPEFLKNKATQGNLVIDYKDWQIPLGRRFRSLKLWMVLR 370
              C +     R   I+ LS+N E+L   A++   ++  ++   PL        LW  L 
Sbjct: 319 PMPCGVQIT--RMEHIKVLSSNVEYL---ASRDATIMGSRNGHAPLF-------LWYTLN 366

Query: 371 LYGLEGLQSHIRSHIAMAAYFEE 393
             G +G Q  ++  +  A Y ++
Sbjct: 367 RKGYKGFQKEVQKCLRNAHYLKD 389