Miyakogusa Predicted Gene

Lj1g3v3690180.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v3690180.1 tr|D0VY45|D0VY45_LOTJA Homocitrate synthase
OS=Lotus japonicus GN=Fen1 PE=2
SV=1,87.91,0,AIPM_HOMOCIT_SYNTH_1,Alpha-isopropylmalate/homocitrate
synthase, conserved site; AIPM_HOMOCIT_SYNTH_,CUFF.31214.1
         (547 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G74040.1 | Symbols: IMS1, MAML-3, IPMS2 | 2-isopropylmalate s...   732   0.0  
AT1G18500.1 | Symbols: MAML-4, IPMS1 | methylthioalkylmalate syn...   720   0.0  
AT5G23010.1 | Symbols: MAM1, IMS3 | methylthioalkylmalate syntha...   532   e-151
AT5G23020.1 | Symbols: MAM-L, MAM3, IMS2 | 2-isopropylmalate syn...   529   e-150

>AT1G74040.1 | Symbols: IMS1, MAML-3, IPMS2 | 2-isopropylmalate
           synthase 1 | chr1:27842258-27845566 FORWARD LENGTH=631
          Length = 631

 Score =  732 bits (1889), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 353/545 (64%), Positives = 431/545 (79%), Gaps = 13/545 (2%)

Query: 15  RNRPEYIPGRIPDSTYVRILDTTLRDGEQAPGAAMTRDQKLEVARQLVKLGVDIIEAGFP 74
           R RP YIP RI D  YVRI DTTLRDGEQ+PGA +T  +KL++ARQL KLGVDIIEAGFP
Sbjct: 71  RRRPNYIPNRISDPNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFP 130

Query: 75  SASKEDFMAVKMIAEEVGNLTDDDGYVPVIGGVCRCNEKDIATTWEALKHAKRPRILTFI 134
           +ASK+DF AVK IAE VGN  D++GYVPVI G+ RCN+KDI T WEA+K+AKRPRI TFI
Sbjct: 131 AASKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIETAWEAVKYAKRPRIHTFI 190

Query: 135 ATSPIHMELKLRKSKDQVLEIARDMVKYARSLGCTDIGFAAEDAARSDREFLYQILGEVI 194
           ATS IH++ KL+KSK++V+EIAR+MV++ARSLGC D+ F+ EDA RS+RE+LY+ILGEVI
Sbjct: 191 ATSDIHLKYKLKKSKEEVIEIARNMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVI 250

Query: 195 KAGATTLTIPDTVGIAMPFEYGKLIADIKENTPGIENAIISTHCHNDLGLATSNTIEGAR 254
           KAGATTL IPDTVGI +P E+G+LIADIK NTPGI+N IISTHC NDLGL+T+NT+ GA 
Sbjct: 251 KAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQNDLGLSTANTLSGAH 310

Query: 255 YGARQLEVTINGIGERAGNASLEEVVMALTCRGNDILGGLHTRINTRHILKTSKMVEKYS 314
            GARQ+EVTINGIGERAGNASLEEVVMA+ CRG+ +LGGL T I+TRHI+ TSKMVE+Y+
Sbjct: 311 SGARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYT 370

Query: 315 GLHLQPHKALVGANAFLHESGIHQDGMLKHRGTYEIISPEDIGLDRSVGDTIVLGKLSGR 374
           G+  QPHKA+VGANAF HESGIHQDGMLKH+GTYEI+SPE+IGL+RS    IVLGKLSGR
Sbjct: 371 GMQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGR 430

Query: 375 QALRNRLEELGYKLKDTEVESVFWQFKAVAETKKRITDADLRALVSNEALDAQPIWRLGD 434
            AL++RL ELGY L D ++ ++FW+FKAVAE KKR+TDADL ALVS+E    + +W+L D
Sbjct: 431 HALKDRLNELGYVLDDGQLSNLFWRFKAVAEQKKRVTDADLIALVSDEVFQPEAVWKLLD 490

Query: 435 LQVTCGTVGFSTATVKLFSIDGSMHFACSIGTGPVDSAYKAINHIVKEPVKLVKYTLGAI 494
           +Q+TCGT+G ST+TVKL   DG  H ACS+GTGPVD+AYKA++ IVKEP  L++Y++ A+
Sbjct: 491 MQITCGTLGLSTSTVKLADSDGKEHVACSVGTGPVDAAYKAVDLIVKEPATLLEYSMNAV 550

Query: 495 TGCMDATATTSVEISHEDMNHP------------IFXXXXXXXXXXXXXXEAYLSALNNM 542
           T  +DA ATT V I   D N+              F              +AY+ ALN M
Sbjct: 551 TEGIDAIATTRVLI-RGDNNYSSTNAVTGESVERTFSGTGAGMDIVVSSVKAYVGALNKM 609

Query: 543 LRFYQ 547
           L F +
Sbjct: 610 LGFKE 614


>AT1G18500.1 | Symbols: MAML-4, IPMS1 | methylthioalkylmalate
           synthase-like 4 | chr1:6369347-6372861 FORWARD
           LENGTH=631
          Length = 631

 Score =  720 bits (1858), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 345/541 (63%), Positives = 425/541 (78%), Gaps = 13/541 (2%)

Query: 19  EYIPGRIPDSTYVRILDTTLRDGEQAPGAAMTRDQKLEVARQLVKLGVDIIEAGFPSASK 78
           EYIP RI D  YVR+ DTTLRDGEQ+PGA +T  +KL++ARQL KLGVDIIEAGFP+ASK
Sbjct: 77  EYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASK 136

Query: 79  EDFMAVKMIAEEVGNLTDDDGYVPVIGGVCRCNEKDIATTWEALKHAKRPRILTFIATSP 138
           +DF AVK IAE VGN  D++GYVPVI G+ RCN+KDI   W+A+K+AKRPRI TFIATS 
Sbjct: 137 DDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIATSD 196

Query: 139 IHMELKLRKSKDQVLEIARDMVKYARSLGCTDIGFAAEDAARSDREFLYQILGEVIKAGA 198
           IH+E KL+K+K +V+EIAR MV++ARSLGC D+ F+ EDA RS+RE+LY+ILGEVIKAGA
Sbjct: 197 IHLEYKLKKTKAEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGA 256

Query: 199 TTLTIPDTVGIAMPFEYGKLIADIKENTPGIENAIISTHCHNDLGLATSNTIEGARYGAR 258
           TTL IPDTVGI +P E+G+LI D+K NTPGIEN +ISTHC NDLGL+T+NT+ GA  GAR
Sbjct: 257 TTLNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAGAR 316

Query: 259 QLEVTINGIGERAGNASLEEVVMALTCRGNDILGGLHTRINTRHILKTSKMVEKYSGLHL 318
           Q+EVTINGIGERAGNASLEEVVMA+ CRG+ +LGGL T I+TRHI+ TSKMVE+Y+G+  
Sbjct: 317 QMEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQT 376

Query: 319 QPHKALVGANAFLHESGIHQDGMLKHRGTYEIISPEDIGLDRSVGDTIVLGKLSGRQALR 378
           QPHKA+VGANAF HESGIHQDGMLKH+GTYEII PE+IGL+RS    IVLGKLSGR AL+
Sbjct: 377 QPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALK 436

Query: 379 NRLEELGYKLKDTEVESVFWQFKAVAETKKRITDADLRALVSNEALDAQPIWRLGDLQVT 438
           +RL ELGY+L D ++ ++FW+FK VAE KKR+TDAD+ ALVS+E    + +W+L D+Q+T
Sbjct: 437 DRLTELGYQLDDEQLSTIFWRFKTVAEQKKRVTDADIIALVSDEVFQPEAVWKLLDIQIT 496

Query: 439 CGTVGFSTATVKLFSIDGSMHFACSIGTGPVDSAYKAINHIVKEPVKLVKYTLGAITGCM 498
           CGT+G STATVKL   DG  H ACSIGTGPVDSAYKA++ IVKEP  L++Y++ A+T  +
Sbjct: 497 CGTLGLSTATVKLADADGKEHVACSIGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGI 556

Query: 499 DATATTSV------------EISHEDMNHPIFXXXXXXXXXXXXXXEAYLSALNNMLRFY 546
           DA ATT V             I+ E++    F              +AY+ ALN M+ F 
Sbjct: 557 DAIATTRVLIRGSNKYSSTNAITGEEVQR-TFSGTGAGMDIVVSSVKAYVGALNKMMDFK 615

Query: 547 Q 547
           +
Sbjct: 616 E 616


>AT5G23010.1 | Symbols: MAM1, IMS3 | methylthioalkylmalate synthase
           1 | chr5:7703173-7706769 FORWARD LENGTH=506
          Length = 506

 Score =  532 bits (1370), Expect = e-151,   Method: Compositional matrix adjust.
 Identities = 245/405 (60%), Positives = 326/405 (80%), Gaps = 1/405 (0%)

Query: 18  PEYIPGRIPDSTYVRILDTTLRDGEQAPGAAMTRDQKLEVARQLVKLGVDIIEAGFPSAS 77
           PEYIP ++PD  YVR+ DTTLRDGEQ+PG ++T  QKLE+ARQL KL VDI+E GFP +S
Sbjct: 72  PEYIPNKLPDGNYVRVFDTTLRDGEQSPGGSLTPPQKLEIARQLAKLRVDIMEVGFPGSS 131

Query: 78  KEDFMAVKMIAEEVGNLTDDD-GYVPVIGGVCRCNEKDIATTWEALKHAKRPRILTFIAT 136
           +E+   +K IA+ VGN  D++ GYVPVI  + RC  +DI  TWEALK+AKRPRIL F +T
Sbjct: 132 EEELETIKTIAKTVGNEVDEETGYVPVICAIARCKHRDIEATWEALKYAKRPRILVFTST 191

Query: 137 SPIHMELKLRKSKDQVLEIARDMVKYARSLGCTDIGFAAEDAARSDREFLYQILGEVIKA 196
           S IHM+ KL+K++++V+E+A   +++A+SLG  DI F  ED  RSD++FL +ILGE IKA
Sbjct: 192 SDIHMKYKLKKTQEEVIEMAVSSIRFAKSLGFNDIQFGCEDGGRSDKDFLCKILGEAIKA 251

Query: 197 GATTLTIPDTVGIAMPFEYGKLIADIKENTPGIENAIISTHCHNDLGLATSNTIEGARYG 256
           G T +TI DTVGI MP EYG+L+  +K NTPGI++ +++ HCHNDLGLAT+N+I G R G
Sbjct: 252 GVTVVTIGDTVGINMPHEYGELVTYLKANTPGIDDVVVAVHCHNDLGLATANSIAGIRAG 311

Query: 257 ARQLEVTINGIGERAGNASLEEVVMALTCRGNDILGGLHTRINTRHILKTSKMVEKYSGL 316
           ARQ+EVTINGIGER+GNASLEEVVMAL CRG  ++ G++T+I+TR I+ TSKMV++Y+GL
Sbjct: 312 ARQVEVTINGIGERSGNASLEEVVMALKCRGAYVINGVYTKIDTRQIMATSKMVQEYTGL 371

Query: 317 HLQPHKALVGANAFLHESGIHQDGMLKHRGTYEIISPEDIGLDRSVGDTIVLGKLSGRQA 376
           ++Q HK +VGAN F+HESGIHQDG+LK+R TYEI+SPEDIG+ +S    +VLGKLSGR A
Sbjct: 372 YVQAHKPIVGANCFVHESGIHQDGILKNRSTYEILSPEDIGIVKSQNSGLVLGKLSGRHA 431

Query: 377 LRNRLEELGYKLKDTEVESVFWQFKAVAETKKRITDADLRALVSN 421
           +++RL+ELGY+L D ++ +VF  F+ + + KKRITDADL+ALV++
Sbjct: 432 VKDRLKELGYELDDEKLNAVFSLFRDLTKNKKRITDADLKALVTS 476


>AT5G23020.1 | Symbols: MAM-L, MAM3, IMS2 | 2-isopropylmalate
           synthase 2 | chr5:7718203-7721634 FORWARD LENGTH=503
          Length = 503

 Score =  529 bits (1362), Expect = e-150,   Method: Compositional matrix adjust.
 Identities = 244/412 (59%), Positives = 330/412 (80%), Gaps = 1/412 (0%)

Query: 13  IKRNRPEYIPGRIPDSTYVRILDTTLRDGEQAPGAAMTRDQKLEVARQLVKLGVDIIEAG 72
           I   RPEYIP ++P   YVR+LDTTLRDGEQ+PGAA+T  QKLE+ARQL KL VDI+E G
Sbjct: 67  IMERRPEYIPNKLPHKNYVRVLDTTLRDGEQSPGAALTPPQKLEIARQLAKLRVDIMEVG 126

Query: 73  FPSASKEDFMAVKMIAEEVGNLTDDD-GYVPVIGGVCRCNEKDIATTWEALKHAKRPRIL 131
           FP +S+E+F A+K IA+ VGN  D++ GYVPVI G+ RC ++DI  TWEALK+AKRPR++
Sbjct: 127 FPVSSEEEFEAIKTIAKTVGNEVDEETGYVPVICGIARCKKRDIEATWEALKYAKRPRVM 186

Query: 132 TFIATSPIHMELKLRKSKDQVLEIARDMVKYARSLGCTDIGFAAEDAARSDREFLYQILG 191
            F +TS IHM+ KL+K+K++V+E+A + VKYA+SLG  DI F  ED  R++++F+ +ILG
Sbjct: 187 LFTSTSEIHMKYKLKKTKEEVIEMAVNSVKYAKSLGFKDIQFGCEDGGRTEKDFICKILG 246

Query: 192 EVIKAGATTLTIPDTVGIAMPFEYGKLIADIKENTPGIENAIISTHCHNDLGLATSNTIE 251
           E IKAGATT+   DTVGI MP E+G+L+A + ENTPG ++ + + HCHNDLG+AT+NTI 
Sbjct: 247 ESIKAGATTVGFADTVGINMPQEFGELVAYVIENTPGADDIVFAIHCHNDLGVATANTIS 306

Query: 252 GARYGARQLEVTINGIGERAGNASLEEVVMALTCRGNDILGGLHTRINTRHILKTSKMVE 311
           G   GARQ+EVTINGIGER+GNA LEEVVMAL CRG  ++ G++T+I++R I+ TSKMV+
Sbjct: 307 GICAGARQVEVTINGIGERSGNAPLEEVVMALKCRGESLMDGVYTKIDSRQIMATSKMVQ 366

Query: 312 KYSGLHLQPHKALVGANAFLHESGIHQDGMLKHRGTYEIISPEDIGLDRSVGDTIVLGKL 371
           +++G+++QPHK +VG N F+HESGIHQDG+LK+R TYEI+SPED+G+ +S    IVLGKL
Sbjct: 367 EHTGMYVQPHKPIVGDNCFVHESGIHQDGILKNRSTYEILSPEDVGIVKSENSGIVLGKL 426

Query: 372 SGRQALRNRLEELGYKLKDTEVESVFWQFKAVAETKKRITDADLRALVSNEA 423
           SGR A+++RL+ELGY++ D +   +F +++ + + KKRITDADL+ALV N A
Sbjct: 427 SGRHAVKDRLKELGYEISDEKFNDIFSRYRELTKDKKRITDADLKALVVNGA 478