Miyakogusa Predicted Gene

Lj1g3v3381400.2

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v3381400.2 tr|I1K753|I1K753_SOYBN Bifunctional dihydrofolate
reductase-thymidylate synthase OS=Glycine max GN=G,86.21,0,seg,NULL;
Thymidylate synthase/dCMP hydroxymethylase,Thymidylate synthase/dCMP
hydroxymethylase doma,CUFF.30578.2
         (524 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G16370.1 | Symbols: THY-1 | thymidylate synthase 1 | chr2:708...   782   0.0  
AT4G34570.1 | Symbols: THY-2 | thymidylate synthase 2 | chr4:165...   777   0.0  
AT2G21550.1 | Symbols:  | Bifunctional dihydrofolate reductase/t...   536   e-153

>AT2G16370.1 | Symbols: THY-1 | thymidylate synthase 1 |
           chr2:7082038-7084333 REVERSE LENGTH=519
          Length = 519

 Score =  782 bits (2020), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 367/500 (73%), Positives = 419/500 (83%), Gaps = 6/500 (1%)

Query: 25  QRTYQVVVAATKDMGIGKDGKLPWRLFTDLKFFKEITTATSDPGKKNAIVMGRKTWESIP 84
           QRTYQVVVAATK+MGIGKDGKLPW L TDLKFFK+IT  TSD  KKNA+VMGRKTWESIP
Sbjct: 19  QRTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDITLTTSDSSKKNAVVMGRKTWESIP 78

Query: 85  LKYRPLPGRLNVVLTRSGSFDIGTAENVVICGSIXXXXXXXXXXPYCLSIEKVFVIGGGQ 144
           +KYRPL GRLNVVLTRSG FDI   ENVV C S+          PYCLSIE+VFVIGGG 
Sbjct: 79  IKYRPLSGRLNVVLTRSGGFDIANTENVVTCSSVDSALDLLAAPPYCLSIERVFVIGGGD 138

Query: 145 IFREVLNAPGCEAIHFTEIHASIECDTFMPPVDSTKYQLWYSSFPKVENDIRYSFATYVR 204
           I RE LN P C+AIH TEI  S++CDTF+P +D++ YQ W SSFP  EN +R+ F T+VR
Sbjct: 139 ILREALNRPSCDAIHLTEIDTSVDCDTFIPAIDTSVYQPWSSSFPVTENGLRFCFTTFVR 198

Query: 205 VQSSAAESLSQNTDPLFDNNSDSLKFEVQNFDFLPEMILQRHEEYMYLRLIQEIISEXXX 264
           V+SSA ES  ++      N S SL+F+ + F FLP+M+  +HEE++YL ++++IIS    
Sbjct: 199 VKSSADESSDES------NGSQSLQFDGKKFLFLPKMVFDQHEEFLYLNMVEDIISNGNV 252

Query: 265 XXXXXXXXXLSKFGCQMRFNLRRSFPLLTTKKVFWRGVVEELLWFISGSTNAKVLQEKGI 324
                    LSKFGCQM+FNLRRSFPLLTTK+VFWRGVVEELLWFISGSTNAKVLQEKGI
Sbjct: 253 KNDRTGTGTLSKFGCQMKFNLRRSFPLLTTKRVFWRGVVEELLWFISGSTNAKVLQEKGI 312

Query: 325 HIWDGNASREYLDSIGLTEREEGDLGPVYGFQWRHFGARYSDMHKDYSGQGYDQLLDVIN 384
           HIWDGNASREYLD IGLTEREEGDLGPVYGFQWRHFGA+Y+DMH DY+GQG+DQL+DVI+
Sbjct: 313 HIWDGNASREYLDGIGLTEREEGDLGPVYGFQWRHFGAKYTDMHADYTGQGFDQLVDVID 372

Query: 385 KIKHNPNDRRIILSAWNPVDLKLMALPPCHMFAQFFVANGELSCQMYQRSADMGLGVPFN 444
           KIK+NP+DRRII+SAWNP DLKLMALPPCHMFAQF+VA GELSCQMYQRSADMGLGVPFN
Sbjct: 373 KIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVAEGELSCQMYQRSADMGLGVPFN 432

Query: 445 IASYALLTCMIAHVCELVPGDFVHVIGDAHVYHNHVRPLQEQLLNLPKPFPTLKINSEKK 504
           IASY+LLTCM+AHVC+LVPGDF+HV+GDAHVY  HVRPLQEQLLNLPKPFP +KIN EKK
Sbjct: 433 IASYSLLTCMLAHVCDLVPGDFIHVLGDAHVYKTHVRPLQEQLLNLPKPFPVMKINPEKK 492

Query: 505 DIDSFVAADFKLVGYNPHQK 524
            IDSFVA+DF L GY+PH+K
Sbjct: 493 QIDSFVASDFDLTGYDPHKK 512


>AT4G34570.1 | Symbols: THY-2 | thymidylate synthase 2 |
           chr4:16511129-16514110 REVERSE LENGTH=565
          Length = 565

 Score =  777 bits (2007), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 369/501 (73%), Positives = 413/501 (82%), Gaps = 6/501 (1%)

Query: 25  QRTYQVVVAATKDMGIGKDGKLPWRLFTDLKFFKEITTATSDPGKKNAIVMGRKTWESIP 84
           Q TYQVVVAATK+MGIGKDGKLPW L TDLKFFK++T +TSD  KKNA+VMGRKTWESIP
Sbjct: 63  QSTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDLTLSTSDSAKKNAVVMGRKTWESIP 122

Query: 85  LKYRPLPGRLNVVLTRSGSFDIGTAENVVICGSIXXXXXXXXXXPYCLSIEKVFVIGGGQ 144
            KYRPL GRLNVVL+RS  FDI   ENVV C SI          P+ LSIEKVFVIGGG 
Sbjct: 123 KKYRPLSGRLNVVLSRSSGFDIANTENVVTCSSIDSALDLLAAPPFSLSIEKVFVIGGGD 182

Query: 145 IFREVLNAPGCEAIHFTEIHASIECDTFMPPVDSTKYQLWYSSFPKVENDIRYSFATYVR 204
           I RE LN P CEAIH TEI  SI+CDTF+P VD++ YQ W SSFP  EN +R+SF T+VR
Sbjct: 183 ILREALNKPSCEAIHITEIDTSIDCDTFIPTVDTSAYQPWCSSFPICENGLRFSFTTHVR 242

Query: 205 VQSSAAESLSQNTDPLFDNNSDSLKFEVQNFD-FLPEMILQRHEEYMYLRLIQEIISEXX 263
           V+SS+A   S  +D      S  L+ + + F   LP+MI  RHEEY+YL L++EIIS   
Sbjct: 243 VKSSSAGEASDESD-----GSKVLQVDWKKFSSVLPKMIFDRHEEYLYLNLVKEIISNGN 297

Query: 264 XXXXXXXXXXLSKFGCQMRFNLRRSFPLLTTKKVFWRGVVEELLWFISGSTNAKVLQEKG 323
                     LSKFGCQM+FNLRR+FPLLTTK+VFWRGVVEELLWFISGSTNAKVLQEKG
Sbjct: 298 LKDDRTGTGTLSKFGCQMKFNLRRNFPLLTTKRVFWRGVVEELLWFISGSTNAKVLQEKG 357

Query: 324 IHIWDGNASREYLDSIGLTEREEGDLGPVYGFQWRHFGARYSDMHKDYSGQGYDQLLDVI 383
           I IWDGNASR YLD IGLTEREEGDLGPVYGFQWRHFGA+Y+DMH DY+GQG+DQLLDVI
Sbjct: 358 IRIWDGNASRAYLDGIGLTEREEGDLGPVYGFQWRHFGAKYTDMHADYTGQGFDQLLDVI 417

Query: 384 NKIKHNPNDRRIILSAWNPVDLKLMALPPCHMFAQFFVANGELSCQMYQRSADMGLGVPF 443
           NKIK+NP+DRRII+SAWNP DLKLMALPPCHMFAQF+VANGELSCQMYQRSADMGLGVPF
Sbjct: 418 NKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPF 477

Query: 444 NIASYALLTCMIAHVCELVPGDFVHVIGDAHVYHNHVRPLQEQLLNLPKPFPTLKINSEK 503
           NIASY+LLTC++AHVC+LVPGDF+HVIGDAHVY NHVRPLQEQL N PKPFP LKIN EK
Sbjct: 478 NIASYSLLTCILAHVCDLVPGDFIHVIGDAHVYKNHVRPLQEQLENPPKPFPVLKINPEK 537

Query: 504 KDIDSFVAADFKLVGYNPHQK 524
           KDIDSFVA DF+L+GY+PH+K
Sbjct: 538 KDIDSFVADDFELIGYDPHKK 558


>AT2G21550.1 | Symbols:  | Bifunctional dihydrofolate
           reductase/thymidylate synthase | chr2:9227209-9230189
           FORWARD LENGTH=492
          Length = 492

 Score =  536 bits (1382), Expect = e-153,   Method: Compositional matrix adjust.
 Identities = 263/494 (53%), Positives = 347/494 (70%), Gaps = 25/494 (5%)

Query: 25  QRTYQVVVAATKDMGIGKDGKLPWRLFTDLKFFKEITTATSDPGKKNAIVMGRKTWESIP 84
           +R+YQVV+AAT+DMG+G D KLPW L ++ +FF+++TT TSDP K+NA +MGRK+WES P
Sbjct: 21  RRSYQVVIAATRDMGLGMDMKLPWDLPSEYQFFQDVTTRTSDPTKRNATIMGRKSWESTP 80

Query: 85  LKYRPLPGRLNVVLTRSGSFDIGTAENVVICGSIXXXXXXXXXXPYCLSIEKVFVIGGGQ 144
           L+ RPLPGRLN+VLT+S   +I   ENV++  S+          PY LSIEKVFVIGGG+
Sbjct: 81  LEIRPLPGRLNIVLTKSSCHNIAIDENVLVSSSMESALELLATEPYSLSIEKVFVIGGGE 140

Query: 145 IFREVLNAPGCEAIHFTEIHASIECDTFMPPVDSTKYQLWYSSFPKVENDIRYSFATYVR 204
           + R  +NA  C+AIH TEI  S+ CD F P VD++ Y+ WYSSFP VEN IRYSF TYVR
Sbjct: 141 LLRNYMNASICDAIHLTEIDISVPCDAFAPRVDTSLYRPWYSSFPVVENGIRYSFNTYVR 200

Query: 205 VQSSAAESLSQNTDPLFDNNSDSLKFEVQNFDFLPEMILQRHEEYMYLRLIQEIISEXXX 264
            + +   S  + +    D         ++ + FLP+M+ +RHEE+ YL L+Q IIS    
Sbjct: 201 RKDAIVGSGEKKSVAESD---------LKEYSFLPKMVFERHEEFGYLNLVQNIISS--- 248

Query: 265 XXXXXXXXXLSKFGCQMRFNLRRSFPLLTTKKVFWRGVVEELLWFISGSTNAKVLQEKGI 324
                    LSKFGCQMRFNLR++FPLLTTKK+FW GVVEE+L  ISGS N K   E G 
Sbjct: 249 -GDMNDNSTLSKFGCQMRFNLRKTFPLLTTKKIFWLGVVEEILQLISGSNNPK---ENGS 304

Query: 325 HIWDGNASREYLDSIGLTEREE-GDLGPVYGFQWRHFGARYSDMHKDYSGQGYDQLLDVI 383
           HIWD + ++EYLDS G+   EE GD   ++G  W+H  AR+         Q + QL DVI
Sbjct: 305 HIWDTDEAKEYLDSFGVNATEEDGDNPFLHGLHWKHCDARFVI-------QEFSQLSDVI 357

Query: 384 NKIKHNPNDRRIILSAWNPVDLKLMALPPCHMFAQFFVANGELSCQMYQRSADMGLGVPF 443
           NKIK+NP+D+RI+L+A NP+D KL ++ PCH F QF+VANGE+SCQ+YQ S +  +G+PF
Sbjct: 358 NKIKNNPHDQRIMLAACNPLDFKL-SVSPCHTFTQFYVANGEVSCQIYQSSTEASIGIPF 416

Query: 444 NIASYALLTCMIAHVCELVPGDFVHVIGDAHVYHNHVRPLQEQLLNLPKPFPTLKINSEK 503
           +IA+Y+LLTC+IAHVC+L  GDF+HVIG A++   HV+ +Q+QL   PKPFP LKIN EK
Sbjct: 417 SIATYSLLTCIIAHVCDLGAGDFIHVIGQAYINKAHVKAIQKQLQISPKPFPILKINPEK 476

Query: 504 KDIDSFVAADFKLV 517
           K +D+F A+D +L+
Sbjct: 477 KKMDNFEASDLELM 490