Miyakogusa Predicted Gene

Lj1g3v2139480.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v2139480.1 tr|G7LFS5|G7LFS5_MEDTR MAC/Perforin domain
containing protein OS=Medicago truncatula
GN=MTR_8g095110,70.82,0,SUBFAMILY NOT NAMED,NULL; FAMILY NOT
NAMED,NULL; MACPF,Membrane attack complex component/perforin
(M,NODE_5738_length_1336_cov_54.420658.path2.1
         (349 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G24290.2 | Symbols:  | MAC/Perforin domain-containing protein...   312   2e-85
AT1G28380.1 | Symbols: NSL1 | MAC/Perforin domain-containing pro...   295   4e-80
AT4G24290.1 | Symbols:  | MAC/Perforin domain-containing protein...   234   6e-62
AT1G14780.1 | Symbols:  | MAC/Perforin domain-containing protein...   234   9e-62
AT1G29690.1 | Symbols: CAD1 | MAC/Perforin domain-containing pro...   220   1e-57

>AT4G24290.2 | Symbols:  | MAC/Perforin domain-containing protein |
           chr4:12594856-12597815 FORWARD LENGTH=606
          Length = 606

 Score =  312 bits (800), Expect = 2e-85,   Method: Compositional matrix adjust.
 Identities = 166/373 (44%), Positives = 228/373 (61%), Gaps = 36/373 (9%)

Query: 9   QAAQKAINSIGLGFDITQDINFDSCKW---ESRLIFVNE--EQCHDLEIPGGVSIPNVPN 63
           +AA+ AI SIG G+D+  D+    CK    +SRL+ + E  + C ++ +PGG+SIPNV  
Sbjct: 9   KAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNC-EIVLPGGISIPNVSK 67

Query: 64  SIKCVRGESIRIQSDVLTSVQMLEHFNQEMGLGGKIASGHFCASFGLPIRYLQDLA-SMS 122
           SIKC +GE +R +SD+L   QM E FNQE+ L GKI SG F A F     + +D A + +
Sbjct: 68  SIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKN 127

Query: 123 FAYDGWFVKCYAIELERYHWELLDHVKEAVPSSWDPEAIARFIKRFGTHVIVGVSMGGKD 182
            A+DG F+  Y++ L++    L +HVK+AVPS+WDP A+ARFI  +GTH+IV V MGGKD
Sbjct: 128 LAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKD 187

Query: 183 VLYVRQEEHASYLDPTSIKKFMTDTAGMVFKDC--------------------------I 216
           V+Y +Q+ H+S L P  ++K + + A   F +                            
Sbjct: 188 VIYAKQQ-HSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFA 246

Query: 217 DTDCLASEDFCNKKENHFVIHSRRGGSSQKMNHSEWLDTIDSEPDVISVLLLPLGHLFKN 276
           DT  L S  + NK++  F+   R G  ++ + H+EWL T+  EPDVIS+  +P+  L   
Sbjct: 247 DTSSLGS--YANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNG 304

Query: 277 IKGGGFVNHALNLYSRYKPPIEALQHFLEFQLPRQWAPVLSETSLGSRRKHQMNIWLRFS 336
           + G GF++HA+NLY RYKPPIE L  FLEFQLPRQWAPV SE  LG +RK Q    L+FS
Sbjct: 305 VPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFS 364

Query: 337 ILGPKLYINTIPV 349
             GPKLY+NT PV
Sbjct: 365 FFGPKLYVNTTPV 377


>AT1G28380.1 | Symbols: NSL1 | MAC/Perforin domain-containing
           protein | chr1:9963696-9966060 FORWARD LENGTH=612
          Length = 612

 Score =  295 bits (754), Expect = 4e-80,   Method: Compositional matrix adjust.
 Identities = 157/371 (42%), Positives = 224/371 (60%), Gaps = 32/371 (8%)

Query: 10  AAQKAINSIGLGFDITQDINFDSCKWE---SRLIFVNEEQCHDLEIPGGVSIPNVPNSIK 66
           AA+KA++ IGLG+D+  D+ F +CK     SRL+ ++  +  DL  PGG+ + NV +SIK
Sbjct: 14  AAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIK 73

Query: 67  CVRGESIRIQSDVLTSVQMLEHFNQEMGLGGKIASGHFCASFGLPIRYLQDLASM-SFAY 125
           C +GE  R++SD+L+  QM E FNQ+M L GKI SG F   F     + +D +S+ + AY
Sbjct: 74  CDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAY 133

Query: 126 DGWFVKCYAIELERYHWELLDHVKEAVPSSWDPEAIARFIKRFGTHVIVGVSMGGKDVLY 185
           DGWF+  Y++E+ R    L D VK  VPSSWD  A+A FI+++GTHV+VGV+MGGKDV++
Sbjct: 134 DGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIH 193

Query: 186 VRQEEHASYLDPTSIKKFMTDTAGMVF-KDCIDTDCLAS----------------EDFCN 228
           V+Q   +++ +P  I+K +       F  D +++   AS                + F  
Sbjct: 194 VKQMRKSNH-EPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPFGT 252

Query: 229 KKENHFVIHS----------RRGGSSQKMNHSEWLDTIDSEPDVISVLLLPLGHLFKNIK 278
              +  V+H+          RRGG     +H  WL T+   P+VIS+  +P+  L   + 
Sbjct: 253 SVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLP 312

Query: 279 GGGFVNHALNLYSRYKPPIEALQHFLEFQLPRQWAPVLSETSLGSRRKHQMNIWLRFSIL 338
           G GF++HA+NLY RYKPPIE L  FLEFQLPRQWAPV  +  LG RR  Q +  L+FS++
Sbjct: 313 GTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLM 372

Query: 339 GPKLYINTIPV 349
           GPKLY+NT  V
Sbjct: 373 GPKLYVNTSKV 383


>AT4G24290.1 | Symbols:  | MAC/Perforin domain-containing protein |
           chr4:12594856-12596590 FORWARD LENGTH=350
          Length = 350

 Score =  234 bits (597), Expect = 6e-62,   Method: Compositional matrix adjust.
 Identities = 129/319 (40%), Positives = 188/319 (58%), Gaps = 36/319 (11%)

Query: 9   QAAQKAINSIGLGFDITQDINFDSCKW---ESRLIFVNE--EQCHDLEIPGGVSIPNVPN 63
           +AA+ AI SIG G+D+  D+    CK    +SRL+ + E  + C ++ +PGG+SIPNV  
Sbjct: 9   KAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNC-EIVLPGGISIPNVSK 67

Query: 64  SIKCVRGESIRIQSDVLTSVQMLEHFNQEMGLGGKIASGHFCASFGLPIRYLQDLA-SMS 122
           SIKC +GE +R +SD+L   QM E FNQE+ L GKI SG F A F     + +D A + +
Sbjct: 68  SIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKN 127

Query: 123 FAYDGWFVKCYAIELERYHWELLDHVKEAVPSSWDPEAIARFIKRFGTHVIVGVSMGGKD 182
            A+DG F+  Y++ L++    L +HVK+AVPS+WDP A+ARFI  +GTH+IV V MGGKD
Sbjct: 128 LAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKD 187

Query: 183 VLYVRQEEHASYLDPTSIKKFMTDTAGMVFKDC--------------------------I 216
           V+Y +Q +H+S L P  ++K + + A   F +                            
Sbjct: 188 VIYAKQ-QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFA 246

Query: 217 DTDCLASEDFCNKKENHFVIHSRRGGSSQKMNHSEWLDTIDSEPDVISVLLLPLGHLFKN 276
           DT  L S  + NK++  F+   R G  ++ + H+EWL T+  EPDVIS+  +P+  L   
Sbjct: 247 DTSSLGS--YANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNG 304

Query: 277 IKGGGFVNHALNLYSRYKP 295
           + G GF++HA+NLY R KP
Sbjct: 305 VPGSGFLSHAINLYLRCKP 323


>AT1G14780.1 | Symbols:  | MAC/Perforin domain-containing protein |
           chr1:5091020-5093873 FORWARD LENGTH=627
          Length = 627

 Score =  234 bits (596), Expect = 9e-62,   Method: Compositional matrix adjust.
 Identities = 137/377 (36%), Positives = 204/377 (54%), Gaps = 40/377 (10%)

Query: 12  QKAINSIGLGFDITQDINFDSCK------WESRLIFVNEEQCHDLEIPGGVSIPNVPNSI 65
           + A+ S+G GFD+T D     CK       + RL+ +++ Q  +L IPG     NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 66  KCVRGESIRIQSDVLTSVQMLEHFNQEMGLGGKIASGHFCASFGLPI-RYLQDLASM-SF 123
            C +GE  R +SD+L   +M E+FNQ   + GKI SG+F A+FG     +  D A++ S 
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 124 AYDGWFVKCYAIELER-YHWELLDHVKEAVPSSWDPEAIARFIKRFGTHVIVGVSMGGKD 182
             D   V  + + +       L D V+ AVPSSWDP+ +ARFI+R+GTHVI GVS+GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 183 VLYVRQEEHASYLDPTSIKKFMTDTAGMVFK-DCI------------------------- 216
           V+ VRQ++ +S LD   ++  + D    +F   C+                         
Sbjct: 190 VVVVRQDK-SSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNV 248

Query: 217 --DTDCLASEDFCNKKENHF-VIHSRRGGSSQKMNHSEWLDTIDSEPDVISVLLLPLGHL 273
             D   +A  +F    +N   VI ++RGG  +  +HSEWL T+  +PD I+   +P+  L
Sbjct: 249 FDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSL 308

Query: 274 FKNIKGGGFVNHALNLYSRYKPPIEALQHFLEFQLPRQWAPVLSETSLGSRRKHQMNI-W 332
            K++ G G ++HA++LY RYKPP+  LQ+FL+F  PR WAPV ++   G+          
Sbjct: 309 LKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPA 368

Query: 333 LRFSILGPKLYINTIPV 349
           L  + +GPKLY+NT PV
Sbjct: 369 LHINFMGPKLYVNTTPV 385


>AT1G29690.1 | Symbols: CAD1 | MAC/Perforin domain-containing
           protein | chr1:10379310-10381861 REVERSE LENGTH=561
          Length = 561

 Score =  220 bits (560), Expect = 1e-57,   Method: Compositional matrix adjust.
 Identities = 122/338 (36%), Positives = 190/338 (56%), Gaps = 10/338 (2%)

Query: 12  QKAINSIGLGFDITQDINFDSCKWE--SRLIFVNEEQCHDLEIPGGVSIPNVPNSIKCVR 69
           + AI ++G GFD+T D+    CK    SRL+ + E Q  DLE+  G  +PNVP  I C R
Sbjct: 22  RNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSR 81

Query: 70  GESIRIQSDVLTSVQMLEHFNQEMGLGGKIASGHFCASFGLPIRYLQDLASM-SFAYDGW 128
           G S   +  V +  +M E FN   G+ G I  G F A F     +  D AS  S A  G+
Sbjct: 82  GNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGY 141

Query: 129 FVKCYAIELERYHWELLDHVKEAVPSSWDPEAIARFIKRFGTHVIVGVSMGGKDVLYVRQ 188
           F+  Y ++L +    L + ++ AVPSSWDP ++A FI+ +GTH++  V++GG+DV+Y+RQ
Sbjct: 142 FIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQ 201

Query: 189 EEHASYLDPTSIKKFMTDTAGMVFKDCIDTDCLASEDFCNKKENHFVIHSRRGGSSQKMN 248
            + +S L  + I+ ++ D     F +           +  K ++  VI  RRGG   + +
Sbjct: 202 HQ-SSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKY--KDKDITVIFRRRGGDDLEQS 258

Query: 249 HSEWLDTIDSEPDVISVLLLPLGHLFKNIKGGGFVNHALNLYSRYKPPIEALQHFLEFQL 308
           H+ W +T+ + PD+I++   P+  L + + G   +  A+ LY  YKPPIE LQ+FL++Q+
Sbjct: 259 HARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIEDLQYFLDYQI 318

Query: 309 PRQWAPVLSETSLGSRRKHQMNIWLRFSILGPKLYINT 346
            R WAP  S      +RK  +   L+FS++GPKL+I+ 
Sbjct: 319 ARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFISA 352