Miyakogusa Predicted Gene

Lj1g3v0130120.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v0130120.1 Non Chatacterized Hit- tr|I1LFY1|I1LFY1_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.48450
PE,92.16,0,seg,NULL; Peptidase_M16_C,Peptidase M16, C-terminal;
Peptidase_M16,Peptidase M16, N-terminal; PITRIL,CUFF.25269.1
         (1020 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G42390.1 | Symbols:  | Insulinase (Peptidase family M16) fami...  1628   0.0  
AT5G56730.1 | Symbols:  | Insulinase (Peptidase family M16) prot...    62   2e-09

>AT5G42390.1 | Symbols:  | Insulinase (Peptidase family M16) family
            protein | chr5:16945308-16952647 FORWARD LENGTH=1265
          Length = 1265

 Score = 1628 bits (4217), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 784/1026 (76%), Positives = 887/1026 (86%), Gaps = 7/1026 (0%)

Query: 1    MIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHAPTSTKD-DDDLLPCVLDALNEI 59
            MIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIH+PT TKD +DDL P VLDALNEI
Sbjct: 241  MIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTHTKDSEDDLFPSVLDALNEI 300

Query: 60   AFHPKFLSSRIEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQIKK 119
            AFHPKFLSSR+EKERRAILSELQMMNTIEYRVDCQLLQHLHSENKL +RFPIGLEEQIKK
Sbjct: 301  AFHPKFLSSRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLGRRFPIGLEEQIKK 360

Query: 120  WDADKIRKFHECWYFPANATLYIVGDVDNISKTVYQIEAVFGQTGLDNEKG-SVATPSAF 178
            WD DKIRKFHE WYFPANATLYIVGD+DNI + V+ IEAVFG+ GLDNE   S  +P AF
Sbjct: 361  WDVDKIRKFHERWYFPANATLYIVGDIDNIPRIVHNIEAVFGKNGLDNESTPSSPSPGAF 420

Query: 179  GAMASFLVPKLSVGLGGNSIERSANTMDQSKISKKERHAVRPPVKHNWYLPGSSTNLKPP 238
            GAMA+FLVPKL  GLGG       NT DQSK+ K+ERHA+RPPV+HNW LPG+S +LKPP
Sbjct: 421  GAMANFLVPKLPAGLGGTFSNEKTNTADQSKMIKRERHAIRPPVEHNWSLPGTSVDLKPP 480

Query: 239  QIFQHELLQNFSINMFCKIPVNKVRTYRDLRNVLMKRIFLSALHFRINTRYKSSNPPFTS 298
            QIF+HELLQNF+INMFCKIPV+KV+T+ DLRNVLMKRIFLSALHFRINTRYKSSNPPFTS
Sbjct: 481  QIFKHELLQNFAINMFCKIPVSKVQTFGDLRNVLMKRIFLSALHFRINTRYKSSNPPFTS 540

Query: 299  VELDHSDSGREGCTVTTLTITAEPKNWQNAIRVAVHEVRRLKEFGVTQGELTRYLDALLK 358
            VELDHSDSGREGCTVTTLT+TAEP+NWQNA++VAV EVRRLKEFGVT+GELTRY+DALLK
Sbjct: 541  VELDHSDSGREGCTVTTLTVTAEPQNWQNAVKVAVQEVRRLKEFGVTRGELTRYMDALLK 600

Query: 359  DSEHLAAMIDNVSSVDNLDFIMESDALGHKVMDQRQGHESLLSLAGTVTLDEVNSVGAKV 418
            DSEHLAAMIDNVSSVDNLDFIMESDAL H VMDQ QGHE+L+++AGTVTL+EVN+VGAKV
Sbjct: 601  DSEHLAAMIDNVSSVDNLDFIMESDALSHTVMDQTQGHETLVAVAGTVTLEEVNTVGAKV 660

Query: 419  LEFIADFGXXXXXXXXXXXXXXXXXFHIEGAGETEFKISSTEITDAIKAGLDYPIQPEPE 478
            LEFI+DFG                  H++G GE++F IS  EI +++K+GL  PI+ EPE
Sbjct: 661  LEFISDFGRPTAPLPAAIVACVPTKVHVDGVGESDFNISPDEIIESVKSGLLAPIEAEPE 720

Query: 479  LEVPKELIPSSKLEELKEQRNPTFIPVSPETNATKLHDKETGITRRRLANGIPVNYKISK 538
            LEVPKELI  S+L+EL  QRNP F+P+ P +  TKLHDKETGIT+ RL+NGI VNYK S 
Sbjct: 721  LEVPKELISQSQLKELTLQRNPCFVPI-PGSGLTKLHDKETGITQLRLSNGIAVNYKKST 779

Query: 539  SETQSGVMRLIVGGGRAAETPESRGSVIVGVRTLSEGGRVGNFSREQVELFCVNHLINCS 598
            +E+++GVMRLIVGGGRAAET +S+G+V+VGVRTLSEGGRVG+FSREQVELFCVNHLINCS
Sbjct: 780  TESRAGVMRLIVGGGRAAETSDSKGAVVVGVRTLSEGGRVGDFSREQVELFCVNHLINCS 839

Query: 599  LESTEEFISMEFRFTLRDNGMRAAFQLLHMVLENSVWLDDAFDRARQLYLSYYRSIPKSL 658
            LESTEEFI+MEFRFTLRDNGM+AAFQLLHMVLE SVWL+DAFDRARQLYLSY+RSIPKSL
Sbjct: 840  LESTEEFIAMEFRFTLRDNGMQAAFQLLHMVLERSVWLEDAFDRARQLYLSYFRSIPKSL 899

Query: 659  ERSTAHKLMVAMLDGDERFIEPTPKSLENLTLQTVKDAVMNQFVGDNMEVSIVGDFTEEE 718
            ER+TAHKLM+AML+GDERF+EPTPKSL++L L++VKDAVM+ FVGDNMEVSIVGDF+EEE
Sbjct: 900  ERATAHKLMIAMLNGDERFVEPTPKSLQSLNLESVKDAVMSHFVGDNMEVSIVGDFSEEE 959

Query: 719  IESCILDYLGTAQATRNFKREQEFYPPSFRSSPSDLLSQEVFLKDTDERACAYIAGPAPN 778
            IE CILDYLGT +A+ +  +     P  FR   + L  Q+VFLKDTDERACAYIAGPAPN
Sbjct: 960  IERCILDYLGTVKASHDSAKPPGSEPILFRQPTAGLQFQQVFLKDTDERACAYIAGPAPN 1019

Query: 779  RWGFTVDGKDLLESINNAATINDDGAKSDAPQAGG----LQRSLRGHPLFFGITMGLLSE 834
            RWGFTVDG DL +S++     +D   KS+     G    LQ+ LR HPLFFG+TMGLL+E
Sbjct: 1020 RWGFTVDGDDLFQSVSKLPVAHDGLLKSEEQLLEGGDRELQKKLRAHPLFFGVTMGLLAE 1079

Query: 835  IINSRLFTNVRDSLGLTYDVSFELNLFDRLKLGWYVISVTSTPSKVHKAVDACKNVLRGL 894
            IINSRLFT VRDSLGLTYDVSFELNLFDRL LGWYVISVTSTP KV+KAVDACK+VLRGL
Sbjct: 1080 IINSRLFTTVRDSLGLTYDVSFELNLFDRLNLGWYVISVTSTPGKVYKAVDACKSVLRGL 1139

Query: 895  HSNKITDRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDISCIKDLTFLYDA 954
            HSN+I  RELDRAKRTLLMRHEAE+KSNAYWL LLAHLQASSVPRK++SCIK+L  LY+A
Sbjct: 1140 HSNQIAPRELDRAKRTLLMRHEAELKSNAYWLNLLAHLQASSVPRKELSCIKELVSLYEA 1199

Query: 955  ATIEDIYRAYEQLKVDENSLYSCIGIAGAEAVQDIAAPLVEEEPIDTYPGVIPVGRGLST 1014
            A+IEDIY AY QL+VDE+SLYSCIGIAGA+A ++I     EEEP D + GV+PVGRG S 
Sbjct: 1200 ASIEDIYLAYNQLRVDEDSLYSCIGIAGAQAGEEITVLSEEEEPEDVFSGVVPVGRGSSM 1259

Query: 1015 MTRPTT 1020
             TRPTT
Sbjct: 1260 TTRPTT 1265


>AT5G56730.1 | Symbols:  | Insulinase (Peptidase family M16) protein
           | chr5:22946906-22952576 REVERSE LENGTH=956
          Length = 956

 Score = 62.0 bits (149), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 76/340 (22%), Positives = 135/340 (39%), Gaps = 78/340 (22%)

Query: 1   MIEHVAFLGSKKRE-----KLLGT-----GARSNAYTDFHHTVFHIHAPTSTKDDDDLLP 50
           ++EH+AF  + +       K L +     G   NA T    T++ +  P    D  +LL 
Sbjct: 86  IVEHLAFSATTRYTNHDIVKFLESIGAEFGPCQNAMTTADETIYELFVPV---DKPELLS 142

Query: 51  CVLDALNEIAFHPKFLSSRIEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFP 110
             +  L E +   +     +EKER A++ E +       R+     Q +   +K ++R P
Sbjct: 143 QAISILAEFSSEIRVSKEDLEKERGAVMEEYRGNRNATGRMQDSHWQLMMEGSKYAERLP 202

Query: 111 IGLEEQIKKWDADKIRKFHECWYFPANATLYIVGDVDNISKTVYQIEAVFGQTGLDNEKG 170
           IGLE+ I+   A  +++F++ WY   N  +  VGD  +    V  I     +T  ++++ 
Sbjct: 203 IGLEKVIRSVPAATVKQFYQKWYHLCNMAVVAVGDFPDTKTVVDLI-----KTHFEDKRS 257

Query: 171 SVATPSAFGAMASFLVPKLSVGLGGNSIERSANTMDQSKISK-KERHAVRPPVKHNWYLP 229
           S   P     +  F VP                + ++++ S   E  A    V  ++ +P
Sbjct: 258 SSEPPQ----IPVFPVP----------------SHEETRFSCFVESEAAGSAVMISYKMP 297

Query: 230 GSSTNLKPPQIFQHELLQNFSINMFCKIPVNKVRTYRDLRNVLMKRIFLSALHFRINTRY 289
                                        V+ ++T +D R++L + +FL AL+ R+    
Sbjct: 298 -----------------------------VSDLKTVKDYRDMLAESMFLHALNQRLFKIS 328

Query: 290 KSSNPPF--TSVELD--------HSDSGREGCTVTTLTIT 319
           +  +PPF   SV  D        H  S RE   V  L ++
Sbjct: 329 RRKDPPFFACSVAADVLVARVRLHGFSEREISVVRALMMS 368