Miyakogusa Predicted Gene

Lj0g3v0252379.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0252379.1 Non Chatacterized Hit- tr|I1J7P0|I1J7P0_SOYBN
Uncharacterized protein OS=Glycine max PE=4
SV=1,80.53,0,MACPF,Membrane attack complex component/perforin (MACPF)
domain; membrane-attack complex / perforin,,CUFF.16554.1
         (584 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G29690.1 | Symbols: CAD1 | MAC/Perforin domain-containing pro...   692   0.0  
AT4G24290.2 | Symbols:  | MAC/Perforin domain-containing protein...   439   e-123
AT1G28380.1 | Symbols: NSL1 | MAC/Perforin domain-containing pro...   395   e-110
AT1G14780.1 | Symbols:  | MAC/Perforin domain-containing protein...   382   e-106
AT4G24290.1 | Symbols:  | MAC/Perforin domain-containing protein...   221   9e-58

>AT1G29690.1 | Symbols: CAD1 | MAC/Perforin domain-containing
           protein | chr1:10379310-10381861 REVERSE LENGTH=561
          Length = 561

 Score =  692 bits (1785), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 343/575 (59%), Positives = 419/575 (72%), Gaps = 33/575 (5%)

Query: 10  TATNALQALGRGFDVNFDTRLLYCKGVSGSRLVEIDEEHPRDLCLYEDVVVPNVSRDIGS 69
           T  NA+QALGRGFDV  D RLLYCKG  GSRLV I+E   RDL L    ++PNV  DI  
Sbjct: 20  TLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDC 79

Query: 70  SPESNERQSSGVCSFQEMVEYFNRKANISGSYPLGSFNSAFSFTGSKYVDDVATKTLSSD 129
           S  ++  Q   VCSF EM E FN ++ + G+ PLG FN+ F++TGS  VD  +TK+L+  
Sbjct: 80  SRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALV 139

Query: 130 GFFIPLAKLQLNKPRLLLQENVKRAIPVNWDPPSLASFIENFGTHVITSITIGGKDVIYV 189
           G+FIPL  ++L K  L+L   ++RA+P +WDP SLASFIEN+GTH++TS+TIGG+DV+Y+
Sbjct: 140 GYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYI 199

Query: 190 KQHNTSPLSRLEMKNYIRDIGNQRFSDINSHTSLGQTKSKDKGVDPFSFNSQGIYPQPTT 249
           +QH +SPL   E++NY+ D+   RF +  S +  G  K KDK                  
Sbjct: 200 RQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDK------------------ 241

Query: 250 ATYPTGKEDVTVIFRRRGGDDLEQNHSIWINTVKSSPDIIEMTFCPITDLLDEVPGKEHL 309
                   D+TVIFRRRGGDDLEQ+H+ W  TV ++PDII MTF PI  LL+ VPG  HL
Sbjct: 242 --------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 293

Query: 310 THAIGLYLEYKPPIEELRYFLEFQIPRVWAPMHDRIPGQQRKEPVCPSLQFSIMGQKLYV 369
           T AI LYLEYKPPIE+L+YFL++QI R WAP    +   QRKEPVC SLQFS+MG KL++
Sbjct: 294 TRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL---QRKEPVCSSLQFSLMGPKLFI 350

Query: 370 SQEQITVGRRPVTGIRLCLEGSKHNRLSVHLQHLVSLPKILQPYWDSHVAIGAPKWQGPE 429
           S +Q+TVGR+PVTG+RL LEGSK NRLS+HLQHLVSLPKILQP+WDSHV IGAPKWQGPE
Sbjct: 351 SADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPE 410

Query: 430 EQDSRWFEPVKWKNFSHVSTAPVENPETFIADFSGVCVVTGAQLGVWDFGSRNVLYMKLL 489
           EQDSRWFEP+KWKNFSHVST+P+E+ ET I D SGV +VTGAQLGVWDFGS+NVL++KLL
Sbjct: 411 EQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLL 470

Query: 490 YSRLPGCTIRRSLWDHIPNKPLKTVSVGNTSNPDESSISSRENTTTGNKLVKYVDLSEMS 549
           +S++PGCTIRRS+WDH P      +  G    P  SS +   +  +G KL K VD SEM 
Sbjct: 471 FSKVPGCTIRRSVWDHTPVASSGRLEPG---GPSTSSSTEEVSGQSG-KLAKIVDSSEML 526

Query: 550 KGPQDPPGHWLVTGGKLGVEKGKIVLRLKYSLLNY 584
           KGPQD PGHWLVTG KLGVEKGKIVLR+KYSLLNY
Sbjct: 527 KGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561


>AT4G24290.2 | Symbols:  | MAC/Perforin domain-containing protein |
           chr4:12594856-12597815 FORWARD LENGTH=606
          Length = 606

 Score =  439 bits (1128), Expect = e-123,   Method: Compositional matrix adjust.
 Identities = 252/613 (41%), Positives = 366/613 (59%), Gaps = 53/613 (8%)

Query: 1   MAEHVAALHTATNALQALGRGFDVNFDTRLLYCKGVS-GSRLVEIDE-EHPRDLCLYEDV 58
           MA  + A   A  A+ ++G G+D+  D RL YCKG S  SRL++I E +   ++ L   +
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 59  VVPNVSRDIGSSPESNERQSSGVCSFQEMVEYFNRKANISGSYPLGSFNSAFSFTGSKYV 118
            +PNVS+ I        R  S +  FQ+M E FN++ +++G  P G FN+ F F+     
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 119 DDVATKTLSSDGFFIPLAKLQLNKPRLLLQENVKRAIPVNWDPPSLASFIENFGTHVITS 178
           D   TK L+ DG FI L  + L+K ++LL+E+VK+A+P  WDP +LA FI+ +GTH+I S
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 179 ITIGGKDVIYVKQHNTSPLSRLEMKNYIRDIGNQRFSDINSHTSLG--------QTKSKD 230
           + +GGKDVIY KQ ++S L   +++  ++++ ++RF + +   + G        + ++K+
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE 240

Query: 231 KGVDPFSFNSQGIYPQPTTATYPTGKEDVTVIFRRRGGDDLEQ-NHSIWINTVKSSPDII 289
           + +     +S G Y           KED   + +RRGG+D     H+ W+ TV+  PD+I
Sbjct: 241 QRLRFADTSSLGSY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVI 291

Query: 290 EMTFCPITDLLDEVPGKEHLTHAIGLYLEYKPPIEELRYFLEFQIPRVWAPMHDRIP-GQ 348
            M+F PIT LL+ VPG   L+HAI LYL YKPPIEEL  FLEFQ+PR WAP+   +P G 
Sbjct: 292 SMSFIPITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGP 351

Query: 349 QRKEPVCPSLQFSIMGQKLYVSQEQITVGRRPVTGIRLCLEGSKHNRLSVHLQHLVSLPK 408
           QRK+  C SLQFS  G KLYV+   + VG+RP+TG+RL LEG + NRL++HLQHL SLPK
Sbjct: 352 QRKQQSCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPK 411

Query: 409 ILQPYWDSHVAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPVENPETFIADFSGVCVV 468
           I Q   D + +I        E  D R++E V WKN+SHV T PVE+ +        + VV
Sbjct: 412 IYQLEDDLNRSIRQ------ESHDRRYYEKVNWKNYSHVCTEPVESDDD-------LSVV 458

Query: 469 TGAQLGVWDFGSRNVLYMKLLYSRLPGCT-IRRSLWDH----IPNKPLKTVSVGN----- 518
           TGAQL V   G +NVL+++L +SR+ G T ++ S WD      P   L +  + +     
Sbjct: 459 TGAQLHVESHGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAA 518

Query: 519 ---TSNPDESSISSR------ENTTTGNKLVKYVDLSEMSKGPQDPPGHWLVTGGKLGVE 569
                 P + +I+S          T   KL+K+VD SEM++GPQ+ PG+W+V+G +L VE
Sbjct: 519 QKPPPRPADVNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVE 578

Query: 570 KGKIVLRLKYSLL 582
           KGKI L++KYSL 
Sbjct: 579 KGKISLKVKYSLF 591


>AT1G28380.1 | Symbols: NSL1 | MAC/Perforin domain-containing
           protein | chr1:9963696-9966060 FORWARD LENGTH=612
          Length = 612

 Score =  395 bits (1016), Expect = e-110,   Method: Compositional matrix adjust.
 Identities = 236/609 (38%), Positives = 337/609 (55%), Gaps = 47/609 (7%)

Query: 7   ALHTATNALQALGRGFDVNFDTRLLYCKGV-SGSRLVEIDEEHPRDLCLYEDVVVPNVSR 65
           A   A  A+  +G G+D+  D R   CK    GSRLVEID    RDL     +VV NVS 
Sbjct: 11  AHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSS 70

Query: 66  DIGSSPESNERQSSGVCSFQEMVEYFNRKANISGSYPLGSFNSAFSFTGSKYVDDVATKT 125
            I        R  S + SF +M E FN+   +SG  P G FN+ F+F+     D  + KT
Sbjct: 71  SIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKT 130

Query: 126 LSSDGFFIPLAKLQLNKPRLLLQENVKRAIPVNWDPPSLASFIENFGTHVITSITIGGKD 185
           L+ DG+FI L  +++ + +L L++ VKR +P +WD  +LA FIE +GTHV+  +T+GGKD
Sbjct: 131 LAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKD 190

Query: 186 VIYVKQHNTSPLSRLEMKNYIRDIGNQRFS----DINSHTSLGQTKSKDK-----GVDPF 236
           VI+VKQ   S     E++  ++  G++RF     +  S  S+   K K++     G+ PF
Sbjct: 191 VIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPF 250

Query: 237 SFNSQGIYPQPTTATYPTGKEDVTVIFRRRGGDDLEQNHSIWINTVKSSPDIIEMTFCPI 296
             +        +     T  E++  +  RRGG DL Q+H  W++TV  +P++I M F PI
Sbjct: 251 GTSVS------SAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPI 304

Query: 297 TDLLDEVPGKEHLTHAIGLYLEYKPPIEELRYFLEFQIPRVWAPMHDRIP-GQQRKEPVC 355
           T LL  +PG   L+HA+ LYL YKPPIEEL  FLEFQ+PR WAP++  +P G +R +   
Sbjct: 305 TSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSS 364

Query: 356 PSLQFSIMGQKLYVSQEQITVGRRPVTGIRLCLEGSKHNRLSVHLQHLVSLPKILQ-PYW 414
           PSLQFS+MG KLYV+  ++  G RPVTG+R  LEG K N L++HLQHL + P  L   + 
Sbjct: 365 PSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHD 424

Query: 415 DSHVAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPVENPETFIADFSGVCVVTGAQLG 474
           D++  I  P  +G       ++ PVKW  FSHV T PV+       D +   +VT A L 
Sbjct: 425 DTYEPIEEPVEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLE 475

Query: 475 VWDFGSRNVLYMKLLYSRLPGCTIRRSLWDHIPNKPLKT---------VSVGNTSNPDES 525
           V   G R VL+++L +S       R+S WD++     K+         +S G + NP  +
Sbjct: 476 VKGMGMRKVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATT 535

Query: 526 SISSRENTTTG-----------NKLVKYVDLSEMSKGPQDPPGHWLVTGGKLGVEKGKIV 574
              S+ +  +             KL+  VD  E+ +GP++ PG+W+VTG KL VE GKI 
Sbjct: 536 KPQSKIDINSAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKIS 595

Query: 575 LRLKYSLLN 583
           ++ KYSLL 
Sbjct: 596 IKAKYSLLT 604


>AT1G14780.1 | Symbols:  | MAC/Perforin domain-containing protein |
           chr1:5091020-5093873 FORWARD LENGTH=627
          Length = 627

 Score =  382 bits (980), Expect = e-106,   Method: Compositional matrix adjust.
 Identities = 228/621 (36%), Positives = 345/621 (55%), Gaps = 64/621 (10%)

Query: 14  ALQALGRGFDVNFDTRLLYCKGVSGS----RLVEIDEEHPRDLCLYEDVVVPNVSRDIGS 69
           A+++LG+GFD+  D RL YCK   GS    RLV +D+   R+L +    V  NVS DI  
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 70  SPESNERQSSGVCSFQEMVEYFNRKANISGSYPLGSFNSAFSFTGSKYVDDVA-TKTLSS 128
                 R  S +  F +M EYFN++++++G  P G+FN+ F F    +  D A  K+L  
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 129 DGFFIPLAKLQLNKP-RLLLQENVKRAIPVNWDPPSLASFIENFGTHVITSITIGGKDVI 187
           D   + L  L ++ P RL L + V+ A+P +WDP  LA FIE +GTHVIT +++GG+DV+
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 188 YVKQHNTSPLSRLEMKNYIRDIGNQRFSDINSHTSLGQTKSKDKGVD--------PFSFN 239
            V+Q  +S L    +++++ D+G+Q F+     + L  T+  +K           P +FN
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLFTG----SCLLSTRRLNKAYHHSHSQPKFPEAFN 247

Query: 240 SQGIYPQPTTATYPT----GKEDVTVIFRRRGGDDLEQNHSIWINTVKSSPDIIEMTFCP 295
              ++    T  +       +  +TVI  +RGGD   ++HS W+ TV   PD I   F P
Sbjct: 248 ---VFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIP 304

Query: 296 ITDLLDEVPGKEHLTHAIGLYLEYKPPIEELRYFLEFQIPRVWAPMHDRIP--GQQRKEP 353
           IT LL +VPG   L+HA+ LYL YKPP+ +L+YFL+F  PR WAP+H+ +P         
Sbjct: 305 ITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMAS 364

Query: 354 VCPSLQFSIMGQKLYVSQEQITVGRRPVTGIRLCLEGSKHNRLSVHLQHL-VSLPKILQP 412
             P+L  + MG KLYV+   +T  + PVTG+R  LEG K NRL++HLQHL  +   + + 
Sbjct: 365 AYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEK 424

Query: 413 YWDSHVAIGAPKWQGPEE--QDSRWFEPVKWKNFSHVSTAPVENPETFIADFSG------ 464
             D H+      W+G ++   + R+FEP+  K FSHV T PV+    +I   S       
Sbjct: 425 ITDEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQND 478

Query: 465 -VCVVTGAQLGVWDFGSRNVLYMKLLYSRLPGCTIRRSLWDHIP-------------NKP 510
              +VTGAQL V   GS++VL+++L Y+++    + ++ W H P             + P
Sbjct: 479 VAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMP 538

Query: 511 LKTVSV-GNTSNPDESSISSRENTTTG-------NKLVKYVDLSEMSKGPQDPPGHWLVT 562
           L + SV  N    D++ +        G       NK+VK+VDLS++ +GPQ  PGHWLVT
Sbjct: 539 LTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLVT 598

Query: 563 GGKLGVEKGKIVLRLKYSLLN 583
           G +L ++KGK+ L +K++LL+
Sbjct: 599 GVRLYLDKGKLCLHVKFALLH 619


>AT4G24290.1 | Symbols:  | MAC/Perforin domain-containing protein |
           chr4:12594856-12596590 FORWARD LENGTH=350
          Length = 350

 Score =  221 bits (564), Expect = 9e-58,   Method: Compositional matrix adjust.
 Identities = 130/346 (37%), Positives = 200/346 (57%), Gaps = 24/346 (6%)

Query: 1   MAEHVAALHTATNALQALGRGFDVNFDTRLLYCKGVS-GSRLVEIDE-EHPRDLCLYEDV 58
           MA  + A   A  A+ ++G G+D+  D RL YCKG S  SRL++I E +   ++ L   +
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 59  VVPNVSRDIGSSPESNERQSSGVCSFQEMVEYFNRKANISGSYPLGSFNSAFSFTGSKYV 118
            +PNVS+ I        R  S +  FQ+M E FN++ +++G  P G FN+ F F+     
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 119 DDVATKTLSSDGFFIPLAKLQLNKPRLLLQENVKRAIPVNWDPPSLASFIENFGTHVITS 178
           D   TK L+ DG FI L  + L+K ++LL+E+VK+A+P  WDP +LA FI+ +GTH+I S
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 179 ITIGGKDVIYVKQHNTSPLSRLEMKNYIRDIGNQRFSDINSHTSLG--------QTKSKD 230
           + +GGKDVIY KQ ++S L   +++  ++++ ++RF + +   + G        + ++K+
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE 240

Query: 231 KGVDPFSFNSQGIYPQPTTATYPTGKEDVTVIFRRRGGDDLEQ-NHSIWINTVKSSPDII 289
           + +     +S G Y           KED   + +RRGG+D     H+ W+ TV+  PD+I
Sbjct: 241 QRLRFADTSSLGSY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVI 291

Query: 290 EMTFCPITDLLDEVPGKEHLTHAIGLYLEYKPPIEELR---YFLEF 332
            M+F PIT LL+ VPG   L+HAI LYL  K PI  LR   +FLE 
Sbjct: 292 SMSFIPITSLLNGVPGSGFLSHAINLYLRCK-PINHLRFVHFFLEL 336