Miyakogusa Predicted Gene

Lj0g3v0208009.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0208009.1 tr|G7J608|G7J608_MEDTR Neutral ceramidase
OS=Medicago truncatula GN=MTR_3g079190 PE=4
SV=1,84.98,0,CERAMIDASE,Neutral/alkaline nonlysosomal ceramidase;
Ceramidase_alk,Neutral/alkaline nonlysosomal ce,CUFF.13352.1
         (408 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G38010.1 | Symbols:  | Neutral/alkaline non-lysosomal ceramid...   367   e-101
AT1G07380.1 | Symbols:  | Neutral/alkaline non-lysosomal ceramid...   348   4e-96
AT2G38010.2 | Symbols:  | Neutral/alkaline non-lysosomal ceramid...   344   5e-95
AT5G58980.1 | Symbols:  | Neutral/alkaline non-lysosomal ceramid...   343   2e-94

>AT2G38010.1 | Symbols:  | Neutral/alkaline non-lysosomal ceramidase
           | chr2:15906862-15909867 FORWARD LENGTH=757
          Length = 757

 Score =  367 bits (941), Expect = e-101,   Method: Compositional matrix adjust.
 Identities = 184/346 (53%), Positives = 228/346 (65%), Gaps = 14/346 (4%)

Query: 1   MSRENKLISGDNKGVAARLFEDLFASQXXXXXXX------XXXXVPDIGQ----LVKKAQ 50
           MSR N LISGDNKG AAR  ED F +                  V D  +    L+  A 
Sbjct: 230 MSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRNIPRRVSTIVSDFSRNRDRLLDIAA 289

Query: 51  SIKATGGKDCNQHTSQASKVRKNDGSLFVGSFCQSNVGDVSPNVLGAFCIDTGHPCDFNH 110
           + K++ G   ++     ++VR      FV +FCQSN GDVSPN LG FCIDTG PCDFNH
Sbjct: 290 TYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNH 349

Query: 111 SSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKTAVDLFESASEELTGNIDYRHAYLNFT 170
           S+CNG ++LC GRGPGYPDE  ST+IIGE+QFK AV+LF  A+E+L G I Y+HAYL+F+
Sbjct: 350 STCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFS 409

Query: 171 NIEVELDT----NKVVKTCXXXXXXXXXXXXXXXXXXXXFQQGDTEINPFLKTVRDTLKE 226
           N++V +      ++ VKTC                    F+QGD + N F + VR+ L+ 
Sbjct: 410 NLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRT 469

Query: 227 PSQYQVGCQNPKAVLLSTGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMSGRRLR 286
           P   QV CQ PK +LL TGEM +PY WAP+ILPIQILR+G+L+ILSVPGEFTTM+GRRLR
Sbjct: 470 PGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLR 529

Query: 287 EAVKKTLISSSNGEFNNETHVVVAGLTNTYSQYIATFEEYQQQRYE 332
           +A+K  LISS   EF+N  HVV+AGLTNTYSQYIATFEEY+ QRYE
Sbjct: 530 DAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYE 575


>AT1G07380.1 | Symbols:  | Neutral/alkaline non-lysosomal ceramidase
           | chr1:2264829-2268306 REVERSE LENGTH=779
          Length = 779

 Score =  348 bits (893), Expect = 4e-96,   Method: Compositional matrix adjust.
 Identities = 176/355 (49%), Positives = 222/355 (62%), Gaps = 23/355 (6%)

Query: 1   MSRENKLISGDNKGVAARLFEDLFASQXXXXXXXXXXXVPDIG---------------QL 45
           MSR N LISGDNKG A+R  ED +                +I                +L
Sbjct: 241 MSRTNSLISGDNKGAASRFMEDWYEQNTAERSYSEEFISDEIPRRVSSLIENHQDSHHEL 300

Query: 46  VKKAQSIKATGGKDCNQHTSQASKVR----KNDGSLFVGSFCQSNVGDVSPNVLGAFCID 101
           ++ A   ++  GK   + +S A +VR    K D   FV +FCQ+N GDVSPNVLGAFC+D
Sbjct: 301 LELASYFESQPGKPVTRISSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLD 360

Query: 102 TGHPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKTAVDLFESASEELTGNID 161
           TG PCDFNHS+C G +++C GRGPGYPDE  ST+IIGERQFK A++LF  ASE+L G +D
Sbjct: 361 TGLPCDFNHSTCGGKNEMCYGRGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVD 420

Query: 162 YRHAYLNFTNIEVEL----DTNKVVKTCXXXXXXXXXXXXXXXXXXXXFQQGDTEINPFL 217
           YRH Y++F+ + V L      ++VVKTC                    F QGD + NPF 
Sbjct: 421 YRHVYVDFSQLNVTLPKKDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFW 480

Query: 218 KTVRDTLKEPSQYQVGCQNPKAVLLSTGEMFDPYPWAPAILPIQILRLGKLIILSVPGEF 277
           + VR+ LK P + Q+ C  PK +LL TGEM  PY WAP+IL +Q+LR+G+L ILSVPGEF
Sbjct: 481 RLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEF 540

Query: 278 TTMSGRRLREAVKKTLISSSNGEFNNETHVVVAGLTNTYSQYIATFEEYQQQRYE 332
           TTM+GRRLR AVK  L +S N + + E HVV+AGL N YSQY+ TFEEYQ QRYE
Sbjct: 541 TTMAGRRLRYAVKTQLKNSGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYE 595


>AT2G38010.2 | Symbols:  | Neutral/alkaline non-lysosomal ceramidase
           | chr2:15906862-15909867 FORWARD LENGTH=792
          Length = 792

 Score =  344 bits (883), Expect = 5e-95,   Method: Compositional matrix adjust.
 Identities = 164/293 (55%), Positives = 207/293 (70%), Gaps = 4/293 (1%)

Query: 44  QLVKKAQSIKATGGKDCNQHTSQASKVRKNDGSLFVGSFCQSNVGDVSPNVLGAFCIDTG 103
           +L+  A + K++ G   ++     ++VR      FV +FCQSN GDVSPN LG FCIDTG
Sbjct: 318 RLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTG 377

Query: 104 HPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKTAVDLFESASEELTGNIDYR 163
            PCDFNHS+CNG ++LC GRGPGYPDE  ST+IIGE+QFK AV+LF  A+E+L G I Y+
Sbjct: 378 LPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQ 437

Query: 164 HAYLNFTNIEVELDT----NKVVKTCXXXXXXXXXXXXXXXXXXXXFQQGDTEINPFLKT 219
           HAYL+F+N++V +      ++ VKTC                    F+QGD + N F + 
Sbjct: 438 HAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRL 497

Query: 220 VRDTLKEPSQYQVGCQNPKAVLLSTGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTT 279
           VR+ L+ P   QV CQ PK +LL TGEM +PY WAP+ILPIQILR+G+L+ILSVPGEFTT
Sbjct: 498 VRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTT 557

Query: 280 MSGRRLREAVKKTLISSSNGEFNNETHVVVAGLTNTYSQYIATFEEYQQQRYE 332
           M+GRRLR+A+K  LISS   EF+N  HVV+AGLTNTYSQYIATFEEY+ QRYE
Sbjct: 558 MAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYE 610


>AT5G58980.1 | Symbols:  | Neutral/alkaline non-lysosomal ceramidase
           | chr5:23811449-23814395 REVERSE LENGTH=733
          Length = 733

 Score =  343 bits (879), Expect = 2e-94,   Method: Compositional matrix adjust.
 Identities = 176/338 (52%), Positives = 218/338 (64%), Gaps = 19/338 (5%)

Query: 11  DNKGVAARLFEDLFASQ--------XXXXXXXXXXXVPDIGQLVKKAQSIKATGGKDCNQ 62
           D  G  AR+ ED F  +                    P    L++ A S+ +TGGK   +
Sbjct: 215 DQWGPVARIMEDWFERENGCRSVDVESPRRVSSIISDPYDQDLMEMASSLLSTGGKTVTR 274

Query: 63  HTSQASKVRKN----DGSLFVGSFCQSNVGDVSPNVLGAFCIDTGHPCDFNHSSCNGNDQ 118
            +S A +VR      D   FV +FCQ+N GDVSPNVLGAFCIDTG PC+FN S+C G ++
Sbjct: 275 MSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNE 334

Query: 119 LCVGRGPGYPDEILSTKIIGERQFKTAVDLFESASEELTGNIDYRHAYLNFTNIEVELD- 177
            C GRGPGYPDE  ST+IIGERQFK A DLF  ASEE+ G +DYRHAY++F+ +EV ++ 
Sbjct: 335 QCYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTING 394

Query: 178 ---TNKVVKTCXXXXXXXXXXXXXXXXXXXXFQQGDTEINPFLKTVRDTLKEPSQYQVGC 234
               ++VVKTC                    F+QGD + NPF + VR+ LK P++ QV C
Sbjct: 395 QNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRC 454

Query: 235 QNPKAVLLSTGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMSGRRLREAVKKTLI 294
           Q PK +LL TGEM  PY WAP+ILP+QILR+G+L+IL VPGEFTTM+GRRLR+AVK  L 
Sbjct: 455 QRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLK 514

Query: 295 SSSNGEFNNETHVVVAGLTNTYSQYIATFEEYQQQRYE 332
             SNG    E  VV+AGLTN+YSQYIATFEEYQ QRYE
Sbjct: 515 EGSNGR---EFSVVIAGLTNSYSQYIATFEEYQVQRYE 549