Miyakogusa Predicted Gene

Lj0g3v0136369.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0136369.1 Non Chatacterized Hit- tr|I1LNG1|I1LNG1_SOYBN
Uncharacterized protein OS=Glycine max PE=4
SV=1,81.1,0,ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE,NULL;
no description,Aldolase-type TIM barrel; n,CUFF.8459.1
         (455 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G26380.1 | Symbols:  | Melibiase family protein | chr3:966014...   539   e-153
AT5G08370.2 | Symbols: AtAGAL2, AGAL2 | alpha-galactosidase 2 | ...    69   8e-12
AT5G08370.1 | Symbols: AtAGAL2, AGAL2 | alpha-galactosidase 2 | ...    69   9e-12
AT3G56310.2 | Symbols:  | Melibiase family protein | chr3:208828...    67   2e-11
AT3G56310.1 | Symbols:  | Melibiase family protein | chr3:208828...    67   2e-11
AT5G08380.1 | Symbols: AtAGAL1, AGAL1 | alpha-galactosidase 1 | ...    59   8e-09

>AT3G26380.1 | Symbols:  | Melibiase family protein |
           chr3:9660140-9663145 FORWARD LENGTH=647
          Length = 647

 Score =  539 bits (1389), Expect = e-153,   Method: Compositional matrix adjust.
 Identities = 265/462 (57%), Positives = 332/462 (71%), Gaps = 15/462 (3%)

Query: 1   MSHGFMSVNTQFGAGKAFLRSLYEQYAAWGVDFVKHDCVFGDDLDLNEISYVSEVLRELG 60
           MSHGFMSVNT+ GAGKAFLRSLY QYA WGVDF+KHDCVFG D ++ EI+YVSEVL+EL 
Sbjct: 190 MSHGFMSVNTKLGAGKAFLRSLYRQYAEWGVDFIKHDCVFGTDFNIEEITYVSEVLKELD 249

Query: 61  RPIVYSLSPGTSVTPAMAKDISGLVNMYRITADDWDNWGDVKTHFDVTRDFSTANMIGAK 120
           RP++YS+SPGTSVTP MAK++S LVNMYRIT DDWD W DV  HFD++RD S ++MIGA+
Sbjct: 250 RPVLYSISPGTSVTPTMAKEVSQLVNMYRITGDDWDTWKDVTAHFDISRDLSASSMIGAR 309

Query: 121 GLMGNSWPDLDMLPFGWLTDPGSNEGPHRYTNLNLQEQRTQMTLWSMAKTPLMYGGDVRK 180
           GL G SWPDLDMLP GWLTD GSN GPHR  NLNL+EQ++QMTLWS+AK+PLM+GGDVR 
Sbjct: 310 GLQGKSWPDLDMLPLGWLTDQGSNVGPHRACNLNLEEQKSQMTLWSIAKSPLMFGGDVRN 369

Query: 181 IDSTTYDLITNPTLLEINSFSSNNMELPYITILKSEDQDLGRQMRRSYRE----KETSYK 236
           +D+TTY+LITNPTLLEINS+SSNN E PYIT  +     + R   + Y      K  S K
Sbjct: 370 LDATTYNLITNPTLLEINSYSSNNKEFPYITATR-----VSRNKHKGYPHHPTGKNISTK 424

Query: 237 YSLGLTSCSESKASGWTSESYNGYLERICWKRSFKNKHLAPFCVQKRELNLTFKKESMYH 296
           ++ GLTSC E KA+ W     N    +ICW +    K   PFC+  R+  L   K+  ++
Sbjct: 425 HAFGLTSCKEQKANTWFIVDKN--RGQICWNQHSSEKLEKPFCLYNRKALLASDKKLKHN 482

Query: 297 EDRQRGHHLVAANRIKFCLDASPRRKLSSLEFRRCTFSPCRLDSNQMWELNPNGTLVNSY 356
           +  Q   HL   ++ + CL AS ++KL+S ++ +   SPC+LD+NQMWEL+ NGTL NSY
Sbjct: 483 QLYQGKLHLHTNDKAQSCLAASSQQKLTSKDYSQGALSPCKLDANQMWELHSNGTLENSY 542

Query: 357 SGLCATMESVKAT-ITSGGFRSWIATGRAGKVYVAFFNLSEQKAVMSVQTSDLAKVLPGK 415
           SGLCA +  VKA   +S G RSWIATGR G+VYVAFFNL+++K  +S + SD+A  L GK
Sbjct: 543 SGLCAVLNPVKAAEASSNGVRSWIATGRRGEVYVAFFNLNQEKTKISAKISDIATALRGK 602

Query: 416 D---FSSCKGSEIWSGSDTVIMQGTLSTAVEVHGSALFVLNC 454
                +SC   E+WSG D    + ++S  VE HG ALFVL+C
Sbjct: 603 KNLVGASCTSHELWSGKDFGPTKDSVSIQVEPHGPALFVLHC 644


>AT5G08370.2 | Symbols: AtAGAL2, AGAL2 | alpha-galactosidase 2 |
           chr5:2691116-2693441 REVERSE LENGTH=370
          Length = 370

 Score = 68.9 bits (167), Expect = 8e-12,   Method: Compositional matrix adjust.
 Identities = 51/175 (29%), Positives = 82/175 (46%), Gaps = 25/175 (14%)

Query: 26  YAAWGVDFVKHD-CVFGDDLDLNEISYVSEVLRELGRPIVYSLSPGTSVTPAM-AKDISG 83
           +A+WG+D++K+D C             +S+ L   GR I +SL       PA  A DI  
Sbjct: 124 FASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG- 182

Query: 84  LVNMYRITADDWDNWGDVKTHFDVTRDFSTANMIGAKGLMGNSWPDLDMLPFGWLTDPGS 143
             N +R T D  DNW  +    D    +++    G       SW D DML  G       
Sbjct: 183 --NSWRTTGDIQDNWKSMTLIADQNDRWASYARPG-------SWNDPDMLEVG------- 226

Query: 144 NEGPHRYTNLNLQEQRTQMTLWSMAKTPLMYGGDVRKIDSTTYDLITNPTLLEIN 198
           N G      +  +E  +  ++W++AK PL+ G D+R +D  T++L++N  ++ +N
Sbjct: 227 NGG------MTKEEYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVN 275


>AT5G08370.1 | Symbols: AtAGAL2, AGAL2 | alpha-galactosidase 2 |
           chr5:2691116-2693441 REVERSE LENGTH=396
          Length = 396

 Score = 68.6 bits (166), Expect = 9e-12,   Method: Compositional matrix adjust.
 Identities = 51/175 (29%), Positives = 82/175 (46%), Gaps = 25/175 (14%)

Query: 26  YAAWGVDFVKHD-CVFGDDLDLNEISYVSEVLRELGRPIVYSLSPGTSVTPAM-AKDISG 83
           +A+WG+D++K+D C             +S+ L   GR I +SL       PA  A DI  
Sbjct: 150 FASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG- 208

Query: 84  LVNMYRITADDWDNWGDVKTHFDVTRDFSTANMIGAKGLMGNSWPDLDMLPFGWLTDPGS 143
             N +R T D  DNW  +    D    +++    G       SW D DML  G       
Sbjct: 209 --NSWRTTGDIQDNWKSMTLIADQNDRWASYARPG-------SWNDPDMLEVG------- 252

Query: 144 NEGPHRYTNLNLQEQRTQMTLWSMAKTPLMYGGDVRKIDSTTYDLITNPTLLEIN 198
           N G      +  +E  +  ++W++AK PL+ G D+R +D  T++L++N  ++ +N
Sbjct: 253 NGG------MTKEEYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVN 301


>AT3G56310.2 | Symbols:  | Melibiase family protein |
           chr3:20882886-20885745 FORWARD LENGTH=413
          Length = 413

 Score = 67.4 bits (163), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 53/177 (29%), Positives = 85/177 (48%), Gaps = 29/177 (16%)

Query: 26  YAAWGVDFVKHDCVFGDDLDLNEISY---VSEVLRELGRPIVYSLSPGTSVTPAM-AKDI 81
           +A+WGVD++K+D  F  +L +  I     + + L   GR I YSL       PA+ AK++
Sbjct: 158 FASWGVDYLKYDNCF--NLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPALWAKEV 215

Query: 82  SGLVNMYRITADDWDNWGDVKTHFDVTRDFSTANMIGAKGLMGNSWPDLDMLPFGWLTDP 141
               N +R T D  D W  + T  D+   +  A   G  G     W D DML  G     
Sbjct: 216 G---NSWRTTDDINDTWASMTTIADLNNKW--AAYAGPGG-----WNDPDMLEIG----- 260

Query: 142 GSNEGPHRYTNLNLQEQRTQMTLWSMAKTPLMYGGDVRKIDSTTYDLITNPTLLEIN 198
             N G      +  +E R   ++W++ K PL+ G DVR + + T ++++N  ++ +N
Sbjct: 261 --NGG------MTYEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVN 309


>AT3G56310.1 | Symbols:  | Melibiase family protein |
           chr3:20882886-20885745 FORWARD LENGTH=437
          Length = 437

 Score = 67.4 bits (163), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 53/177 (29%), Positives = 85/177 (48%), Gaps = 29/177 (16%)

Query: 26  YAAWGVDFVKHDCVFGDDLDLNEISY---VSEVLRELGRPIVYSLSPGTSVTPAM-AKDI 81
           +A+WGVD++K+D  F  +L +  I     + + L   GR I YSL       PA+ AK++
Sbjct: 182 FASWGVDYLKYDNCF--NLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPALWAKEV 239

Query: 82  SGLVNMYRITADDWDNWGDVKTHFDVTRDFSTANMIGAKGLMGNSWPDLDMLPFGWLTDP 141
               N +R T D  D W  + T  D+   +  A   G  G     W D DML  G     
Sbjct: 240 G---NSWRTTDDINDTWASMTTIADLNNKW--AAYAGPGG-----WNDPDMLEIG----- 284

Query: 142 GSNEGPHRYTNLNLQEQRTQMTLWSMAKTPLMYGGDVRKIDSTTYDLITNPTLLEIN 198
             N G      +  +E R   ++W++ K PL+ G DVR + + T ++++N  ++ +N
Sbjct: 285 --NGG------MTYEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVN 333


>AT5G08380.1 | Symbols: AtAGAL1, AGAL1 | alpha-galactosidase 1 |
           chr5:2694851-2697616 REVERSE LENGTH=410
          Length = 410

 Score = 58.9 bits (141), Expect = 8e-09,   Method: Compositional matrix adjust.
 Identities = 46/176 (26%), Positives = 78/176 (44%), Gaps = 23/176 (13%)

Query: 24  EQYAAWGVDFVKHD-CVFGDDLDLNEISYVSEVLRELGRPIVYSLSPGTSVTPAMAKDIS 82
           + +A WG+D++K+D C             ++  L + GRPI +SL     + PA+     
Sbjct: 162 KTFAEWGIDYLKYDNCNSDGSKPTVRYPVMTRALMKSGRPIFHSLCEWGDMHPALWGSPV 221

Query: 83  GLVNMYRITADDWDNWGDVKTHFDVTRDFSTANMIGAKGLMGNSWPDLDMLPFGWLTDPG 142
           G  N +R T D  D W  + +  D+       N + A+      W D DML  G      
Sbjct: 222 G--NSWRTTNDIKDTWLSMISIADM-------NEVYAEHARPGGWNDPDMLEVG------ 266

Query: 143 SNEGPHRYTNLNLQEQRTQMTLWSMAKTPLMYGGDVRKIDSTTYDLITNPTLLEIN 198
            N G      +   E     ++W+++K PL+ G D+R +   T +++ N  ++ IN
Sbjct: 267 -NGG------MTKDEYIVHFSIWAISKAPLLLGCDIRNMTKETMEIVANKEVIAIN 315