Miyakogusa Predicted Gene

Lj0g3v0067399.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0067399.1 Non Chatacterized Hit- tr|F0ZAP4|F0ZAP4_DICPU
Putative uncharacterized protein OS=Dictyostelium
purp,36.31,1e-18,S5A_REDUCTASE,3-oxo-5-alpha-steroid 4-dehydrogenase,
C-terminal; 3-OXO-5-ALPHA-STEROID 4-DEHYDROGENA,CUFF.3192.1
         (270 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G16010.1 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase ...   314   5e-86
AT2G38050.1 | Symbols: DET2, DWF6, ATDET2 | 3-oxo-5-alpha-steroi...    77   1e-14
AT3G55360.1 | Symbols: CER10, ECR, ATTSC13, TSC13 | 3-oxo-5-alph...    65   6e-11
AT1G72590.1 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase ...    64   9e-11
AT2G16530.1 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase ...    59   5e-09
AT2G16530.2 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase ...    58   5e-09

>AT5G16010.1 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase
           family protein | chr5:5227982-5229012 FORWARD LENGTH=268
          Length = 268

 Score =  314 bits (804), Expect = 5e-86,   Method: Compositional matrix adjust.
 Identities = 150/252 (59%), Positives = 194/252 (76%), Gaps = 1/252 (0%)

Query: 20  VTAMSVLSFVALGNSGFSEIRGNHLNYSKFWNANTGGAAAAQRQVKLSSRTGMLWLYTPA 79
           +  MSV+   AL N G+SEIRGNHL YSKF  +++      +R   +SSR GML LYTPA
Sbjct: 17  LNCMSVVGVAALANIGWSEIRGNHLKYSKFGVSSSSPQPQKERFGSISSRNGMLLLYTPA 76

Query: 80  FLAGAASFWVFPDEGLRSTLVQSAVTLHFLKRVLEVLFVHKYSGSMLLDSAIPITTSYFM 139
           FLA A+SF+V P + LR  L++SA+ LHF KRV EVLF+HKYSG M +DSA+ I++SYF 
Sbjct: 77  FLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYSGGMAIDSALTISSSYFS 136

Query: 140 SAVTMIYAQHLTRELPEPPVNLMYPGIVLFLVGIIGNFYHHYLLSKLRGK-GEKEYKIPK 198
           S   M+Y+Q+LT  L EP  ++   G+V+F+VGI+GN YHH LL+KLR + G+KEYKIPK
Sbjct: 137 STALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLLAKLRKEDGKKEYKIPK 196

Query: 199 GGLFDLVVCPHYLFEIIVFYGVSFMSQTLYSVSFAVGTTFYLLGRSYATRKWYLSKFEDF 258
           GGLFD+++CPHYLFEI+VF+    +SQT+YS SFA+GT  YL+GRSYATR WYLSKF+DF
Sbjct: 197 GGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIGRSYATRTWYLSKFDDF 256

Query: 259 PNHVKAVIPFLF 270
           P H+KA+IPF+F
Sbjct: 257 PKHIKALIPFVF 268


>AT2G38050.1 | Symbols: DET2, DWF6, ATDET2 | 3-oxo-5-alpha-steroid
           4-dehydrogenase family protein | chr2:15921303-15922176
           REVERSE LENGTH=262
          Length = 262

 Score = 77.0 bits (188), Expect = 1e-14,   Method: Compositional matrix adjust.
 Identities = 40/107 (37%), Positives = 64/107 (59%), Gaps = 1/107 (0%)

Query: 165 GIVLFLVGIIGNFYHHYLLSKLRGKGEKEYKIPKGGLFDLVVCPHYLFEIIVFYGVSFMS 224
           G+V+F+ G+  N      L +L+ +    Y IP+GG F+LV CP+Y  E I + G + M+
Sbjct: 156 GMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMT 215

Query: 225 QTLYSVSFAVGTTFYLLGRSYATRKWYLSKF-EDFPNHVKAVIPFLF 270
            +   + F + T   L  R+ A+ KWY++KF E++P   KAVIPF++
Sbjct: 216 WSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY 262


>AT3G55360.1 | Symbols: CER10, ECR, ATTSC13, TSC13 |
           3-oxo-5-alpha-steroid 4-dehydrogenase family protein |
           chr3:20521186-20522856 REVERSE LENGTH=310
          Length = 310

 Score = 64.7 bits (156), Expect = 6e-11,   Method: Compositional matrix adjust.
 Identities = 55/173 (31%), Positives = 82/173 (47%), Gaps = 9/173 (5%)

Query: 105 TLHFLKRVLEVLFVHKYSGSMLLDSAIPITTSYFMSAVTMIYAQHLTRELPEPPVNL-MY 163
             H+ KR+LE  FVH++S +      +    +Y+ S    I A ++   L  P  +L M 
Sbjct: 139 CFHYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYI-AYYVNHPLYTPVSDLQMK 197

Query: 164 PGIVLFLVGIIGNFYHHYLLSKLRG-KGEKEYKIPKGGLFDLVVCPHYLFEIIVFYGVSF 222
            G    LV  + NFY H LL  LR   G   Y+IP+G LF++V C +Y  EI  + G + 
Sbjct: 198 IGFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNI 257

Query: 223 MSQTLYSVSF----AVGTTFYLLGRSYATRKWYLSKF--EDFPNHVKAVIPFL 269
            +QT+    F    A+  T + LG+    RK +  K     +P     + PFL
Sbjct: 258 ATQTIAGYVFLAVAALIMTNWALGKHSRLRKIFDGKDGKPKYPRRWVILPPFL 310


>AT1G72590.1 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase
           family protein | chr1:27335601-27336989 FORWARD
           LENGTH=320
          Length = 320

 Score = 64.3 bits (155), Expect = 9e-11,   Method: Compositional matrix adjust.
 Identities = 54/210 (25%), Positives = 91/210 (43%), Gaps = 36/210 (17%)

Query: 96  RSTLVQSAVTLHFLKRVLEVLFVHKYSGSMLLDSAIPITTSYFMSAVTMIYAQHLTRELP 155
           R+  +   + +H L+RV+E  +V KYS S  +     +   ++  A  +    ++  E+ 
Sbjct: 112 RAVFLLLLMEIHVLRRVIESFYVFKYSTSARMHILAYVGALFYYVAAPLSLCSNIAPEVA 171

Query: 156 E--------------------------PPV----NLMYPGIVLFLVGIIGNFYHHYLLSK 185
                                       P+    +L + G  +FL G I     H +L  
Sbjct: 172 RFVGSQVAEFIASGKSHSHDFNLLLSISPLMKLGSLQWIGGAIFLWGWIHQRRCHAILGS 231

Query: 186 LRG--KGEKEYKIPKGGLFDLVVCPHYLFEIIVFYGVSFMSQTLYSVSFAVGTTFYLLGR 243
           LR      KEY IP G  F++V CPH+L EI+++ G+  +S     +S  +   F     
Sbjct: 232 LREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYLGL-LISSGGTDISIWLLFGFVAANL 290

Query: 244 SYA---TRKWYLSKFEDFPNHVKAVIPFLF 270
           +YA   T +WYL KFE++P    A+ P ++
Sbjct: 291 TYAAGETHRWYLQKFENYPASRHAIFPHVY 320


>AT2G16530.1 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase
           family protein | chr2:7163276-7165064 REVERSE LENGTH=343
          Length = 343

 Score = 58.5 bits (140), Expect = 5e-09,   Method: Compositional matrix adjust.
 Identities = 39/118 (33%), Positives = 60/118 (50%), Gaps = 10/118 (8%)

Query: 160 NLMYPGIVLFLVGIIGNFYHHYLLSKLRGKGE--KEYKIPKGGLFDLVVCPHYLFEIIVF 217
           +L + G  +FL G I     H +L  LR      KEY IP G  F +V  PH+L EI+++
Sbjct: 229 SLQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLY 288

Query: 218 YGVSFMSQ----TLYSV-SFAVGTTFYLLGRSYATRKWYLSKFEDFPNHVKAVIPFLF 270
            G+   S     T++ +  F      Y  G    T +WYL KFE++P +  A+ P+++
Sbjct: 289 AGLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 343


>AT2G16530.2 | Symbols:  | 3-oxo-5-alpha-steroid 4-dehydrogenase
           family protein | chr2:7163276-7165064 REVERSE LENGTH=342
          Length = 342

 Score = 58.2 bits (139), Expect = 5e-09,   Method: Compositional matrix adjust.
 Identities = 39/118 (33%), Positives = 60/118 (50%), Gaps = 10/118 (8%)

Query: 160 NLMYPGIVLFLVGIIGNFYHHYLLSKLRGKGE--KEYKIPKGGLFDLVVCPHYLFEIIVF 217
           +L + G  +FL G I     H +L  LR      KEY IP G  F +V  PH+L EI+++
Sbjct: 228 SLQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLY 287

Query: 218 YGVSFMSQ----TLYSV-SFAVGTTFYLLGRSYATRKWYLSKFEDFPNHVKAVIPFLF 270
            G+   S     T++ +  F      Y  G    T +WYL KFE++P +  A+ P+++
Sbjct: 288 AGLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 342