Miyakogusa Predicted Gene
- Lj0g3v0063869.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj0g3v0063869.1 Non Chatacterized Hit- tr|D7TF60|D7TF60_VITVI
Putative uncharacterized protein OS=Vitis vinifera
GN=,83.41,0,AA_TRNA_LIGASE_I,Aminoacyl-tRNA synthetase, class I,
conserved site; TRNASYNTHVAL,Valine-tRNA ligase,CUFF.2934.1
(426 letters)
Database: TAIR10_pep
35,386 sequences; 14,482,855 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G16715.1 | Symbols: EMB2247 | ATP binding;valine-tRNA ligases... 665 0.0
AT1G14610.1 | Symbols: TWN2, VALRS | valyl-tRNA synthetase / val... 310 2e-84
AT5G49030.1 | Symbols: OVA2 | tRNA synthetase class I (I, L, M a... 103 3e-22
AT5G49030.3 | Symbols: OVA2 | tRNA synthetase class I (I, L, M a... 103 3e-22
AT5G49030.2 | Symbols: OVA2 | tRNA synthetase class I (I, L, M a... 102 4e-22
AT4G10320.1 | Symbols: | tRNA synthetase class I (I, L, M and V... 76 5e-14
AT4G04350.1 | Symbols: EMB2369 | tRNA synthetase class I (I, L, ... 67 2e-11
>AT5G16715.1 | Symbols: EMB2247 | ATP binding;valine-tRNA
ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP
binding;aminoacyl-tRNA ligases | chr5:5485353-5493229
FORWARD LENGTH=974
Length = 974
Score = 665 bits (1716), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 317/393 (80%), Positives = 344/393 (87%), Gaps = 6/393 (1%)
Query: 34 TRRRAIPCCRRFTTTRLLAVAASENGVFTSPEIAKSFDFSAEERIYNWWESQGYFKPTFD 93
+R +I RRF+ + AAS N VFTSPE +K+FDFS+EE+IY WWESQGYFKP FD
Sbjct: 41 SRLNSIFSQRRFS----FSAAASGNNVFTSPETSKTFDFSSEEKIYKWWESQGYFKPNFD 96
Query: 94 RGSDPFVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMKGRPTLWLPGTDHAGIATQLV 153
+G PFVI MPPPNVTGSLHMGHAMFVTLEDIMVRYNRM GRPTLWLPGTDHAGIATQLV
Sbjct: 97 QGGSPFVIPMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMNGRPTLWLPGTDHAGIATQLV 156
Query: 154 VERMLASEGIKRVEMTRDEFTTRVWQWKEKYGGTITNQIKRLGASCDWSREHFTLDEQLS 213
VE+MLASEGIKRV++ RDEFT RVW+WKEKYGGTITNQIKRLGASCDWSRE FTLDEQLS
Sbjct: 157 VEKMLASEGIKRVDLGRDEFTKRVWEWKEKYGGTITNQIKRLGASCDWSRERFTLDEQLS 216
Query: 214 HAVVEAFVRLHEKGLIYQGSYMVNWSPTLQTAVSDLEVEYSEESGYLYYIRYRIAGGSRS 273
AVVEAFV+LH+KGLIYQGSYMVNWSP LQTAVSDLEVEYSEE G+LY+I+YR+AG
Sbjct: 217 RAVVEAFVKLHDKGLIYQGSYMVNWSPNLQTAVSDLEVEYSEEPGFLYHIKYRVAGS--P 274
Query: 274 DYLTVATTRPETLFGDVALAVNPHDDRYSKYIGQMAIVPQTFGRHVPIIYDKHVDKEFGT 333
D+LT+ATTRPETLFGDVALAV+P DDRYSKY+GQ AIVP T+GRHVPII DK+VDK+FGT
Sbjct: 275 DFLTIATTRPETLFGDVALAVHPEDDRYSKYVGQTAIVPMTYGRHVPIIADKYVDKDFGT 334
Query: 334 GVLKISPGHDHNDYLLARKLGLPILNVMNKDGTLNDVAGLYSGLDRFXXXXXXXXXXXXT 393
GVLKISPGHDHNDYLLARKLGLPILNVMNKD TLNDVAGL+ GLDRF
Sbjct: 335 GVLKISPGHDHNDYLLARKLGLPILNVMNKDATLNDVAGLFCGLDRFEVREKLWADLEEI 394
Query: 394 GLAVKKEPHTLRVPRSQRGGEVIEPLVSKQWFV 426
GLAVKKEPHTLRVPRSQRGGEVIEPLVSKQWFV
Sbjct: 395 GLAVKKEPHTLRVPRSQRGGEVIEPLVSKQWFV 427
>AT1G14610.1 | Symbols: TWN2, VALRS | valyl-tRNA synthetase /
valine--tRNA ligase (VALRS) | chr1:5008502-5014486
REVERSE LENGTH=1108
Length = 1108
Score = 310 bits (793), Expect = 2e-84, Method: Compositional matrix adjust.
Identities = 165/384 (42%), Positives = 229/384 (59%), Gaps = 23/384 (5%)
Query: 63 SPEIAKSFD-FSAEERIYNWWESQGYFKPTFDRGSDPFVISMPPPNVTGSLHMGHAMFVT 121
S ++AK + + E+ Y WWE FK PFVI +PPPNVTG+LH+GHA+
Sbjct: 133 SSQMAKQYSPATVEKSWYAWWEKSDLFKADAKSSKPPFVIVLPPPNVTGALHIGHALTSA 192
Query: 122 LEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVER-MLASEGIKRVEMTRDEFTTRVWQW 180
+ED ++R+ RM G LW+PG DHAGIATQ+VVE+ ++ G+ R ++ R+EF VW+W
Sbjct: 193 IEDTIIRWKRMSGYNALWVPGVDHAGIATQVVVEKKIMRDRGMTRHDVGREEFVKEVWKW 252
Query: 181 KEKYGGTITNQIKRLGASCDWSREHFTLDEQLSHAVVEAFVRLHEKGLIYQGSYMVNWSP 240
K +YGGTI Q++RLGAS DWSRE FT+DEQ S AV EAFVRL+++GLIY+ +VNW
Sbjct: 253 KNQYGGTILTQLRRLGASLDWSRECFTMDEQRSKAVTEAFVRLYKEGLIYRDIRLVNWDC 312
Query: 241 TLQTAVSDLEVEYSE----------------ESGYLYYIRYRIAGGSRSDYLTVATTRPE 284
L+TA+SD+EVEY + E G L Y + GG + VATTR E
Sbjct: 313 ILRTAISDVEVEYIDIKEKTLLKVPGYEKPVEFGLLTSFAYPLEGGLGE--VIVATTRVE 370
Query: 285 TLFGDVALAVNPHDDRYSKYIGQMAIVPQTFGRHVPIIYDK-HVDKEFGTGVLKISPGHD 343
T+ GD A+A++P D RY G+ A+ P GR +PII D VD FGTG +KI+P HD
Sbjct: 371 TMLGDTAIAIHPDDARYKHLHGKFAVHPFN-GRKLPIICDGILVDPNFGTGCVKITPAHD 429
Query: 344 HNDYLLARKLGLPILNVMNKDGTLNDVAGL-YSGLDRFXXXXXXXXXXXXTGLAVKKEPH 402
ND + ++ L +N+ DG +N G ++G+ RF GL + +
Sbjct: 430 PNDCEVGKRHKLEFINIFTDDGKINTNGGSDFAGMPRFAAREAVVEALQKQGLYRGAKNN 489
Query: 403 TLRVPRSQRGGEVIEPLVSKQWFV 426
+R+ R +VIEP++ QW+V
Sbjct: 490 EMRLGLCSRTNDVIEPMIKPQWYV 513
>AT5G49030.1 | Symbols: OVA2 | tRNA synthetase class I (I, L, M and
V) family protein | chr5:19875091-19882291 REVERSE
LENGTH=1093
Length = 1093
Score = 103 bits (256), Expect = 3e-22, Method: Compositional matrix adjust.
Identities = 71/224 (31%), Positives = 113/224 (50%), Gaps = 15/224 (6%)
Query: 75 EERIYNWWESQGYFKPTFDRGSD-PFVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMK 133
E + WE FK D + F++ PP G LHMGHA+ L+DI+ RY ++
Sbjct: 123 EPELQKLWEENQVFKRVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQ 182
Query: 134 GRPTLWLPGTDHAGIATQLVVERMLASEGIKRVEMTRDEFTTRVWQWKEKYGGTITNQIK 193
++PG D G+ +L V + L E R E+T + + ++ + T K
Sbjct: 183 NYKVQYVPGWDCHGLPIELKVLQSLDQE--VRKELTPLKLRAKAAKFAKATVKTQMESFK 240
Query: 194 RLGASCDWSREHFTLDEQLSHAVVEAFVRLHEKGLIYQGSYMVNWSPTLQTAVSDLEVEY 253
R G DW+ + TLD + A +E F ++ KG IY+G V+WSP+ +TA+++ E+EY
Sbjct: 241 RFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEY 300
Query: 254 SEE--SGYLYYIRYRIAGGSRSD---------YLTVATTRPETL 286
E S +Y I +++ GG+++ YL V TT P T+
Sbjct: 301 PEGHISKSIYAI-FKLVGGAKTSLLDEFIPNIYLAVWTTTPWTM 343
>AT5G49030.3 | Symbols: OVA2 | tRNA synthetase class I (I, L, M and
V) family protein | chr5:19875091-19883251 REVERSE
LENGTH=1279
Length = 1279
Score = 103 bits (256), Expect = 3e-22, Method: Compositional matrix adjust.
Identities = 71/224 (31%), Positives = 113/224 (50%), Gaps = 15/224 (6%)
Query: 75 EERIYNWWESQGYFKPTFDRGSD-PFVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMK 133
E + WE FK D + F++ PP G LHMGHA+ L+DI+ RY ++
Sbjct: 309 EPELQKLWEENQVFKRVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQ 368
Query: 134 GRPTLWLPGTDHAGIATQLVVERMLASEGIKRVEMTRDEFTTRVWQWKEKYGGTITNQIK 193
++PG D G+ +L V + L E R E+T + + ++ + T K
Sbjct: 369 NYKVQYVPGWDCHGLPIELKVLQSLDQE--VRKELTPLKLRAKAAKFAKATVKTQMESFK 426
Query: 194 RLGASCDWSREHFTLDEQLSHAVVEAFVRLHEKGLIYQGSYMVNWSPTLQTAVSDLEVEY 253
R G DW+ + TLD + A +E F ++ KG IY+G V+WSP+ +TA+++ E+EY
Sbjct: 427 RFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEY 486
Query: 254 SEE--SGYLYYIRYRIAGGSRSD---------YLTVATTRPETL 286
E S +Y I +++ GG+++ YL V TT P T+
Sbjct: 487 PEGHISKSIYAI-FKLVGGAKTSLLDEFIPNIYLAVWTTTPWTM 529
>AT5G49030.2 | Symbols: OVA2 | tRNA synthetase class I (I, L, M and
V) family protein | chr5:19876365-19882291 REVERSE
LENGTH=955
Length = 955
Score = 102 bits (255), Expect = 4e-22, Method: Compositional matrix adjust.
Identities = 71/224 (31%), Positives = 113/224 (50%), Gaps = 15/224 (6%)
Query: 75 EERIYNWWESQGYFKPTFDRGSD-PFVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMK 133
E + WE FK D + F++ PP G LHMGHA+ L+DI+ RY ++
Sbjct: 123 EPELQKLWEENQVFKRVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQ 182
Query: 134 GRPTLWLPGTDHAGIATQLVVERMLASEGIKRVEMTRDEFTTRVWQWKEKYGGTITNQIK 193
++PG D G+ +L V + L E R E+T + + ++ + T K
Sbjct: 183 NYKVQYVPGWDCHGLPIELKVLQSLDQE--VRKELTPLKLRAKAAKFAKATVKTQMESFK 240
Query: 194 RLGASCDWSREHFTLDEQLSHAVVEAFVRLHEKGLIYQGSYMVNWSPTLQTAVSDLEVEY 253
R G DW+ + TLD + A +E F ++ KG IY+G V+WSP+ +TA+++ E+EY
Sbjct: 241 RFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEY 300
Query: 254 SEE--SGYLYYIRYRIAGGSRSD---------YLTVATTRPETL 286
E S +Y I +++ GG+++ YL V TT P T+
Sbjct: 301 PEGHISKSIYAI-FKLVGGAKTSLLDEFIPNIYLAVWTTTPWTM 343
>AT4G10320.1 | Symbols: | tRNA synthetase class I (I, L, M and V)
family protein | chr4:6397526-6404509 REVERSE
LENGTH=1190
Length = 1190
Score = 75.9 bits (185), Expect = 5e-14, Method: Compositional matrix adjust.
Identities = 64/238 (26%), Positives = 103/238 (43%), Gaps = 12/238 (5%)
Query: 67 AKSFDFS-AEERIYNWWESQGYFKPTFDRGSD--PFVISMPPPNVTGSLHMGHAMFVTLE 123
K F F EE + ++W FK R + ++ PP TG H GH + T++
Sbjct: 7 GKEFSFPRQEEDVLSFWTEIDAFKTQLKRTENLPEYIFYDGPPFATGLPHYGHILAGTIK 66
Query: 124 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKR----VEMTRDEFTTRVWQ 179
DI+ RY M G G D G+ + ++R L IKR ++M D++
Sbjct: 67 DIVTRYQTMTGHHVTRRFGWDCHGLPVENEIDRKL---NIKRRDEVIKMGIDKYNEECRS 123
Query: 180 WKEKYGGTITNQIKRLGASCDWSREHFTLDEQLSHAVVEAFVRLHEKGLIYQGSYMVNWS 239
+Y I R G D+ ++ T+D +V F +L EK L+Y+G ++ +S
Sbjct: 124 IVTRYVAEWEKVITRCGRWIDFKNDYKTMDLPFMESVWWVFSQLWEKNLVYRGFKVMPYS 183
Query: 240 PTLQTAVSDLEV--EYSEESGYLYYIRYRIAGGSRSDYLTVATTRPETLFGDVALAVN 295
+T +S+ E Y E + + + G + TT P TL ++AL VN
Sbjct: 184 TGCKTPLSNFEAGQNYKEVPDPEIMVTFPVIGDQDNAAFVAWTTTPWTLPSNLALCVN 241
>AT4G04350.1 | Symbols: EMB2369 | tRNA synthetase class I (I, L, M
and V) family protein | chr4:2128129-2133030 FORWARD
LENGTH=973
Length = 973
Score = 67.4 bits (163), Expect = 2e-11, Method: Compositional matrix adjust.
Identities = 59/200 (29%), Positives = 85/200 (42%), Gaps = 32/200 (16%)
Query: 62 TSPEIAKSFDF-SAEERIYNWWESQGYFKPTFD-RGSDP--FVISMPPPNVTGSLHMGHA 117
TS E+ + + F E + +WE F+ D S P +V+ M P LH+GH
Sbjct: 77 TSIELKRVYPFHEIEPKWQRYWEDNRIFRTPDDVDTSKPKFYVLDMFPYPSGAGLHVGHP 136
Query: 118 MFVTLEDIMVRYNRMKGRPTLWLPGTDHAGI-ATQLVVE-----RMLASEGIKRVEMTRD 171
+ T DI+ R RM+G L G D G+ A Q +E + + I R +
Sbjct: 137 LGYTATDILARLRRMQGYNVLHPMGWDAFGLPAEQYAIETGTHPKTTTLKNIDRFRL--- 193
Query: 172 EFTTRVWQWKEKYGGTITNQIKRLGASCDWSREHFTLDEQLSHAVVEAFVRLHEKGLIYQ 231
Q+K LG S DW RE T + F++L++KGL YQ
Sbjct: 194 -------------------QLKSLGFSYDWDRELSTTEPDYYKWTQWIFLQLYKKGLAYQ 234
Query: 232 GSYMVNWSPTLQTAVSDLEV 251
VNW P L T +++ EV
Sbjct: 235 AEVPVNWCPALGTVLANEEV 254