SLC18A3 ORF clone FHC23839

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC23839

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Clone info.

Species

human

Clone Name

pFN21AE6496 Sequence (with vector) Restriction Map

Product ID

FHC23839

ORF Sequence

DNA sequence (1596 bp)
Protein sequence (532 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6496

Symbol

SLC18A3

Alias : CMS21, VACHT

Description

solute carrier family 18 (vesicular acetylcholine transporter), member 3

Original Clone ID

cp96496

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1596 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 532 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7231.1 3.1e-199 99.8 SLC18A3 CCDS6013.1 8.5e-55 38.3 SLC18A1 CCDS7599.1 1.3e-53 37.7 SLC18A2 CCDS47815.1 1.6e-49 40.0 SLC18A1 CCDS47814.1 4.8e-28 36.0 SLC18A1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011701 93 399 PF07690 Major facilitator superfamily ProfileScan IPR020846 35 481 PS50850 Major facilitator superfamily domain
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 36 LVIVCVALLLDNMLYMVIVPIVP 58 PRIMARY 23 2 151 YDVPLLIGLGVMFASTVLFAFAE 173 PRIMARY 23 3 214 LGVALAFISFGSLVAPPFGGILY 236 SECONDARY 23 4 244 PFLVLAAVSLFDALLLLAVAKPF 266 PRIMARY 23 5 289 PYIAVVAGALTTCNIPLAFLEPT 311 SECONDARY 23 6 329 MAWLPAFVPHVLGVYLTVRLAAR 351 SECONDARY 23 7 355 LQWLYGALGLAVIGASSCIVPAC 377 PRIMARY 23 8 386 SLCGLCFGIALVDTALLPTLAFL 408 PRIMARY 23 9 419 SVYAIADISYSVAYALGPIVAGH 441 PRIMARY 23 10 454 GMGLANLLYAPVLLLLRNVGLLT 476 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003046 532 600336 99.8 100.0 NP_003044 525 193002 38.3 91.4 NP_001129163 525 193002 38.3 91.4 NP_003045 514 193001 37.7 93.4 XP_011542927 497 193002 35.8 91.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6496 Download

>KIEE6496  1596 bp
ATGGAATCCGCGGAACCTGCGGGCCAGGCCCGGGCGGCGGCCACCAAGCTGTCGGAGGCT
GTGGGCGCGGCGCTGCAGGAGCCCCGGCGGCAGAGGCGCCTGGTGCTTGTTATCGTGTGC
GTGGCGCTGTTACTGGACAACATGCTGTACATGGTCATCGTGCCCATAGTGCCCGACTAC
ATCGCCCACATGCGCGGGGGCGGCGAGGGCCCCACCCGGACTCCCGAGGTGTGGGAGCCC
ACCCTGCCGCTGCCCACTCCGGCCAATGCCAGCGCCTACACGGCCAACACCTCGGCGTCC
CCGACAGCTGCGTGGCCAGCGGGCTCAGCCCTTCGGCCCCGCTACCCTACGGAGAGCGAA
GACGTGAAGATCGGGGTGCTGTTTGCTTCCAAGGCTATCCTGCAGCTGCTAGTGAACCCC
TTGAGCGGGCCCTTCATCGACCGCATGAGCTACGACGTGCCGCTGCTGATCGGCCTGGGC
GTCATGTTCGCCTCTACAGTCCTGTTCGCCTTCGCCGAGGACTACGCCACGCTGTTCGCG
GCGCGCAGCCTGCAGGGCCTGGGCTCAGCCTTCGCCGACACGTCTGGCATAGCCATGATC
GCCGATAAGTACCCGGAGGAGCCGGAGCGCAGTCGTGCACTGGGCGTGGCGCTGGCCTTC
ATTAGCTTCGGAAGCCTAGTGGCCCCGCCCTTCGGGGGCATCCTCTATGAGTTCGCCGGC
AAGCGCGTGCCCTTCTTGGTGCTAGCTGCCGTGTCGCTCTTTGACGCGCTGTTGCTGCTG
GCAGTGGCCAAACCCTTCTCGGCGGCTGCACGGGCTCGGGCCAACCTGCCAGTGGGCACT
CCCATCCACCGCCTCATGCTAGACCCCTACATTGCCGTGGTGGCCGGCGCGCTCACCACC
TGTAACATTCCCCTCGCCTTCCTCGAACCCACCATTGCCACGTGGATGAAGCATACGATG
GCGGCTTCCGAGTGGGAGATGGGCATGGCCTGGCTGCCGGCCTTCGTGCCTCATGTGCTG
GGCGTCTACCTCACCGTGCGCCTGGCGGCGCGCTACCCACACCTGCAGTGGCTGTACGGC
GCGCTTGGGCTGGCTGTGATCGGCGCCAGCTCGTGCATCGTGCCCGCCTGCCGCTCCTTC
GCGCCGCTAGTGGTCTCACTATGCGGCCTCTGTTTTGGCATAGCCCTAGTCGACACAGCA
CTGCTGCCCACGCTCGCCTTCCTGGTGGACGTGCGCCATGTCTCAGTCTATGGCAGCGTC
TACGCCATCGCCGACATCTCCTATTCGGTGGCCTACGCGCTCGGGCCCATAGTGGCAGGC
CACATTGTGCACTCGCTGGGCTTTGAGCAGCTCAGCCTTGGCATGGGACTGGCCAACCTG
CTCTATGCTCCCGTCTTGCTGCTGCTCCGCAACGTGGGCCTCCTGACGCGCTCCCGTTCC
GAGCGCGATGTGCTGCTTGATGAGCCACCGCAAGGTCTGTACGATGCGGTGCGCCTGCGT
GAGCGTCCTGTGTCTGGCCAGGACGGCGAGCCTCGCAGCCCGCCTGGCCCTTTTGATGAG
TGCGAGGACGACTACAACTACTACTACACCCGCAGC

ORF Protein

Cloned ORF protein sequence for pFN21AE6496 Download

>KIEE6496  532 aa
MESAEPAGQARAAATKLSEAVGAALQEPRRQRRLVLVIVCVALLLDNMLYMVIVPIVPDY
IAHMRGGGEGPTRTPEVWEPTLPLPTPANASAYTANTSASPTAAWPAGSALRPRYPTESE
DVKIGVLFASKAILQLLVNPLSGPFIDRMSYDVPLLIGLGVMFASTVLFAFAEDYATLFA
ARSLQGLGSAFADTSGIAMIADKYPEEPERSRALGVALAFISFGSLVAPPFGGILYEFAG
KRVPFLVLAAVSLFDALLLLAVAKPFSAAARARANLPVGTPIHRLMLDPYIAVVAGALTT
CNIPLAFLEPTIATWMKHTMAASEWEMGMAWLPAFVPHVLGVYLTVRLAARYPHLQWLYG
ALGLAVIGASSCIVPACRSFAPLVVSLCGLCFGIALVDTALLPTLAFLVDVRHVSVYGSV
YAIADISYSVAYALGPIVAGHIVHSLGFEQLSLGMGLANLLYAPVLLLLRNVGLLTRSRS
ERDVLLDEPPQGLYDAVRLRERPVSGQDGEPRSPPGPFDECEDDYNYYYTRS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6496 Download

>pFN21AE6496  6301 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAATCCGCGGAACCTGCGGGCCAGGCCCGGGC
GGCGGCCACCAAGCTGTCGGAGGCTGTGGGCGCGGCGCTGCAGGAGCCCCGGCGGCAGAG
GCGCCTGGTGCTTGTTATCGTGTGCGTGGCGCTGTTACTGGACAACATGCTGTACATGGT
CATCGTGCCCATAGTGCCCGACTACATCGCCCACATGCGCGGGGGCGGCGAGGGCCCCAC
CCGGACTCCCGAGGTGTGGGAGCCCACCCTGCCGCTGCCCACTCCGGCCAATGCCAGCGC
CTACACGGCCAACACCTCGGCGTCCCCGACAGCTGCGTGGCCAGCGGGCTCAGCCCTTCG
GCCCCGCTACCCTACGGAGAGCGAAGACGTGAAGATCGGGGTGCTGTTTGCTTCCAAGGC
TATCCTGCAGCTGCTAGTGAACCCCTTGAGCGGGCCCTTCATCGACCGCATGAGCTACGA
CGTGCCGCTGCTGATCGGCCTGGGCGTCATGTTCGCCTCTACAGTCCTGTTCGCCTTCGC
CGAGGACTACGCCACGCTGTTCGCGGCGCGCAGCCTGCAGGGCCTGGGCTCAGCCTTCGC
CGACACGTCTGGCATAGCCATGATCGCCGATAAGTACCCGGAGGAGCCGGAGCGCAGTCG
TGCACTGGGCGTGGCGCTGGCCTTCATTAGCTTCGGAAGCCTAGTGGCCCCGCCCTTCGG
GGGCATCCTCTATGAGTTCGCCGGCAAGCGCGTGCCCTTCTTGGTGCTAGCTGCCGTGTC
GCTCTTTGACGCGCTGTTGCTGCTGGCAGTGGCCAAACCCTTCTCGGCGGCTGCACGGGC
TCGGGCCAACCTGCCAGTGGGCACTCCCATCCACCGCCTCATGCTAGACCCCTACATTGC
CGTGGTGGCCGGCGCGCTCACCACCTGTAACATTCCCCTCGCCTTCCTCGAACCCACCAT
TGCCACGTGGATGAAGCATACGATGGCGGCTTCCGAGTGGGAGATGGGCATGGCCTGGCT
GCCGGCCTTCGTGCCTCATGTGCTGGGCGTCTACCTCACCGTGCGCCTGGCGGCGCGCTA
CCCACACCTGCAGTGGCTGTACGGCGCGCTTGGGCTGGCTGTGATCGGCGCCAGCTCGTG
CATCGTGCCCGCCTGCCGCTCCTTCGCGCCGCTAGTGGTCTCACTATGCGGCCTCTGTTT
TGGCATAGCCCTAGTCGACACAGCACTGCTGCCCACGCTCGCCTTCCTGGTGGACGTGCG
CCATGTCTCAGTCTATGGCAGCGTCTACGCCATCGCCGACATCTCCTATTCGGTGGCCTA
CGCGCTCGGGCCCATAGTGGCAGGCCACATTGTGCACTCGCTGGGCTTTGAGCAGCTCAG
CCTTGGCATGGGACTGGCCAACCTGCTCTATGCTCCCGTCTTGCTGCTGCTCCGCAACGT
GGGCCTCCTGACGCGCTCCCGTTCCGAGCGCGATGTGCTGCTTGATGAGCCACCGCAAGG
TCTGTACGATGCGGTGCGCCTGCGTGAGCGTCCTGTGTCTGGCCAGGACGGCGAGCCTCG
CAGCCCGCCTGGCCCTTTTGATGAGTGCGAGGACGACTACAACTACTACTACACCCGCAG
CGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
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