Restriction map for pF1KB9838
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BamHI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BpuAI       
BsaBI       BsaI        BsaWI       BseAI       BsiHKAI     
BsiWI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrDI       BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DrdI        Eam1105I    
EarI        Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HinfI       HpaI        KpnI        Ksp632I     MaeI        
MaeIII      MamI        MfeI        MluI        MroI        
MseI        MunI        NdeI        NheI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmeI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfuI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TfiI        Tru9I       Tsp45I      
Tsp509I     TspRI       Tth111I     Van91I      XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AlwI          342  GGATC                GGATC(4/5)
AlwNI         198  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          297  GGGCCC               GGGCC^C
BanII         297  GGGCCC               G[GA]GC[CT]^C
BbrPI         655  CACGTG               CAC^GTG
BlpI          162  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      162  GCTCAGC              GC^T[ACGT]AGC
BsaAI         655  CACGTG               [CT]AC^GT[GA]
BsgI          823  GTGCAG               GTGCAG(16/14)
BsmAI         261  GTCTC                GTCTC(1/5)
BsmBI         260  CGTCTC               CGTCTC(1/5)
Bsp1407I      923  TGTACA               T^GTACA
BsrGI         923  TGTACA               T^GTACA
BsrI          350  ACTGG                ACTGG(1/-1)
BstAPI        167  GCACGCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         612  CCACCACCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         342  GGATCT               [GA]^GATC[CT]
CelII         162  GCTCAGC              GC^T[ACGT]AGC
DpnII         343  GATC                 ^GATC
DraIII        652  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         921  CCTGTACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII         61  GTCAAC               GT[CT]^[GA]AC
HindII         61  GTCAAC               GT[CT]^[GA]AC
MboI          343  GATC                 ^GATC
MflI          342  GGATCT               [GA]^GATC[CT]
NcoI          618  CCATGG               C^CATGG
NdeII         343  GATC                 ^GATC
NotI          472  GCGGCCGC             GC^GGCCGC
PmaCI         655  CACGTG               CAC^GTG
PmlI          655  CACGTG               CAC^GTG
PpuMI         917  GGGACCT              [GA]G^G[AT]CC[CT]
Sau3AI        343  GATC                 ^GATC
SfaNI         369  GCATC                GCATC(5/9)
SfiI          952  GGCCGGGGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI         222  CACCGGCG             C[GA]^CCGG[CT]G
SspBI         923  TGTACA               T^GTACA
XhoII         342  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

Aor51HI       116  AGCGCT               AGC^GCT
              671  AGCGCT              
AvaII         918  GGACC                G^G[AT]CC
             1018  GGTCC               
BalI           47  TGGCCA               TGG^CCA
              905  TGGCCA              
BsiEI         473  CGGCCG               CG[GA][CT]^CG
             1040  CGGCCG              
BstF5I        753  CATCC                GGATG(2/0)
              353  GGATG               
DdeI          163  CTCAG                C^T[ACGT]AG
              263  CTCAG               
EagI          473  CGGCCG               C^GGCCG
             1040  CGGCCG              
EclXI         473  CGGCCG               C^GGCCG
             1040  CGGCCG              
Eco47III      116  AGCGCT               AGC^GCT
              671  AGCGCT              
Eco52I        473  CGGCCG               C^GGCCG
             1040  CGGCCG              
EcoT14I       618  CCATGG               C^C[AT][AT]GG
             1031  CCAAGG              
FokI          753  CATCC                GGATG(9/13)
              353  GGATG               
HphI           90  GGTGA                GGTGA(8/7)
              372  TCACC               
MaeII          59  ACGT                 A^CGT
              656  ACGT                
MboII          93  GAAGA                GAAGA(8/7)
             1049  TCTTC               
MluNI          47  TGGCCA               TGG^CCA
              905  TGGCCA              
MscI           47  TGGCCA               TGG^CCA
              905  TGGCCA              
NaeI          330  GCCGGC               GCC^GGC
              544  GCCGGC              
NgoMIV        330  GCCGGC               G^CCGGC
              544  GCCGGC              
PvuII         688  CAGCTG               CAG^CTG
              880  CAGCTG              
SmlI          760  CTCAAG               C^T[CT][GA]AG
              781  CTCAAG              
StyI          618  CCATGG               C^C[AT][AT]GG
             1031  CCAAGG              
XcmI           35  CCAGCAGCCCGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              609  CCACCACCACCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AvaI        BanI        BbeI        BbvI        BcnI        
BglI        BmyI        BpmI        BsaHI       BsaJI       
BseRI       BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BsrBI       BsrFI       BssHII      BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DraII       DsaI        EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaII       ItaI        KasI        KspI        MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NlaIII      NlaIV       
RsaI        SacII       Sau96I      ScrFI       SfoI        
SmaI        TaqI        TseI        TthHB8I     XmaCI       
XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com