Restriction map for pF1KE2032
 (Linear) (Six-base) MAPSORT of: KIEE2032.seq  Check: 2472  from: 1  to: 1866



KIEE2032.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:53  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1122    1560    1866 
   Size:       1122     438     306

  Fragments arranged by size: 

                1122     438     306
 
AclI AA'CG_TT

Cuts at:      0    1541    1866 
   Size:       1541     325
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1404    1423    1540    1846    1866 
   Size:       1404      19     117     306      20

  Fragments arranged by size: 

                1404     306     117      20      19
 
AflIII A'CryG_T

Cuts at:      0    1640    1812    1866 
   Size:       1640     172      54

  Fragments arranged by size: 

                1640     172      54
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1714    1866 
   Size:       1714     152
 
AleI CACnn'nnGTG

Cuts at:      0    1813    1866 
   Size:       1813      53
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1164    1866 
   Size:       1164     702
 
ApaLI G'TGCA_C

Cuts at:      0      64    1866 
   Size:         64    1802
 
ApoI r'AATT_y

Cuts at:      0     462    1866 
   Size:        462    1404
 
AvaI C'yCGr_G

Cuts at:      0     287     990    1866 
   Size:        287     703     876

  Fragments arranged by size: 

                 876     703     287
 
BamHI G'GATC_C

Cuts at:      0    1622    1866 
   Size:       1622     244
 
BanI G'GyrC_C

Cuts at:      0     668    1866 
   Size:        668    1198
 
BanII G_rGCy'C

Cuts at:      0     695    1064    1102    1729    1866 
   Size:        695     369      38     627     137

  Fragments arranged by size: 

                 695     627     369     137      38
 
BbeI G_GCGC'C

Cuts at:      0     672    1866 
   Size:        672    1194
 
BbvCI CC'TCA_GC

Cuts at:      0      92     326    1866 
   Size:         92     234    1540

  Fragments arranged by size: 

                1540     234      92
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1480    1829    1866 
   Size:       1480     349      37

  Fragments arranged by size: 

                1480     349      37
 
BclI T'GATC_A

Cuts at:      0    1851    1866 
   Size:       1851      15
 
BglI GCCn_nnn'nGGC

Cuts at:      0     214    1866 
   Size:        214    1652
 
BglII A'GATC_T

Cuts at:      0    1337    1866 
   Size:       1337     529
 
Bme1580I G_kGCm'C

Cuts at:      0      68    1866 
   Size:         68    1798
 
BmgBI CAC'GTC

Cuts at:      0       9     191    1529    1866 
   Size:          9     182    1338     337

  Fragments arranged by size: 

                1338     337     182       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1553    1866 
   Size:       1553     313
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     302    1866 
   Size:        302    1564
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     187     241     588    1060    1462    1866 
   Size:         97      90      54     347     472     402     404

  Fragments arranged by size: 

                 472     404     402     347      97      90      54
 
Bpu10I CC'TnA_GC

Cuts at:      0      92     326     733    1866 
   Size:         92     234     407    1133

  Fragments arranged by size: 

                1133     407     234      92
 
BsaBI GATnn'nnATC

Cuts at:      0    1342    1627    1866 
   Size:       1342     285     239

  Fragments arranged by size: 

                1342     285     239
 
BsaHI Gr'CG_yC

Cuts at:      0     283     669    1866 
   Size:        283     386    1197

  Fragments arranged by size: 

                1197     386     283
 
BsaWI w'CCGG_w

Cuts at:      0    1145    1866 
   Size:       1145     721
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     160     175     190     205     677     707     799     829 
   Size:        160      15      15      15     472      30      92      30

Cuts at:    829    1153    1183    1866 
   Size:        324      30     683

  Fragments arranged by size: 

                 683     472     324     160      92      30      30      30
                  15      15      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      80     160     199     217     411     543     820     889 
   Size:         80      80      39      18     194     132     277      69

Cuts at:    889     901     904    1866 
   Size:         12       3     962

  Fragments arranged by size: 

                 962     277     194     132      80      80      69      39
                  18      12       3
 
BseYI C'CCAG_C

Cuts at:      0    1196    1508    1866 
   Size:       1196     312     358

  Fragments arranged by size: 

                1196     358     312
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     784    1866 
   Size:        213     571    1082

  Fragments arranged by size: 

                1082     571     213
 
BsiEI CG_ry'CG

Cuts at:      0     106    1600    1866 
   Size:        106    1494     266

  Fragments arranged by size: 

                1494     266     106
 
BsiHKAI G_wGCw'C

Cuts at:      0      68    1064    1866 
   Size:         68     996     802

  Fragments arranged by size: 

                 996     802      68
 
BsmI GAATG_Cn'

Cuts at:      0     544    1866 
   Size:        544    1322
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     181    1453    1519    1767    1866 
   Size:        181    1272      66     248      99

  Fragments arranged by size: 

                1272     248     181      99      66
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     695    1064    1102    1729    1866 
   Size:         68     627     369      38     627     137

  Fragments arranged by size: 

                 627     627     369     137      68      38
 
BspEI T'CCGG_A

Cuts at:      0    1145    1866 
   Size:       1145     721
 
BspHI T'CATG_A

Cuts at:      0    1718    1866 
   Size:       1718     148
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1122    1560    1589    1866 
   Size:       1122     438      29     277

  Fragments arranged by size: 

                1122     438     277      29
 
BsrBI CCG'CTC

Cuts at:      0    1596    1866 
   Size:       1596     270
 
BsrDI GCAATG_nn'

Cuts at:      0    1385    1866 
   Size:       1385     481
 
BsrFI r'CCGG_y

Cuts at:      0    1599    1866 
   Size:       1599     267
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     383    1137    1866 
   Size:        383     754     729

  Fragments arranged by size: 

                 754     729     383
 
BstEII G'GTnAC_C

Cuts at:      0     678    1866 
   Size:        678    1188
 
BstYI r'GATC_y

Cuts at:      0    1337    1622    1866 
   Size:       1337     285     244

  Fragments arranged by size: 

                1337     285     244
 
BtgI C'CryG_G

Cuts at:      0     230     491     881    1866 
   Size:        230     261     390     985

  Fragments arranged by size: 

                 985     390     261     230
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     595    1866 
   Size:        595    1271
 
BtsI GCAGTG_nn'

Cuts at:      0     640    1215    1866 
   Size:        640     575     651

  Fragments arranged by size: 

                 651     640     575
 
DraIII CAC_nnn'GTG

Cuts at:      0    1440    1866 
   Size:       1440     426
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1198    1866 
   Size:       1198     668
 
EaeI y'GGCC_r

Cuts at:      0     558     827    1597    1866 
   Size:        558     269     770     269

  Fragments arranged by size: 

                 770     558     269     269
 
EagI C'GGCC_G

Cuts at:      0    1597    1866 
   Size:       1597     269
 
EarI CTCTTCn'nnn_

Cuts at:      0    1144    1717    1866 
   Size:       1144     573     149

  Fragments arranged by size: 

                1144     573     149
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1810    1866 
   Size:       1810      56
 
EcoICRI GAG'CTC

Cuts at:      0    1062    1866 
   Size:       1062     804
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     187     241     588    1060    1404    1423    1462 
   Size:         97      90      54     347     472     344      19      39

Cuts at:   1462    1540    1846    1866 
   Size:         78     306      20

  Fragments arranged by size: 

                 472     347     344     306      97      90      78      54
                  39      20      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1667    1866 
   Size:       1667     199
 
EcoO109I rG'GnC_Cy

Cuts at:      0     496     729     980    1200    1708    1866 
   Size:        496     233     251     220     508     158

  Fragments arranged by size: 

                 508     496     251     233     220     158
 
EcoRV GAT'ATC

Cuts at:      0    1322    1866 
   Size:       1322     544
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1746    1866 
   Size:       1746     120
 
FspI TGC'GCA

Cuts at:      0     154    1273    1866 
   Size:        154    1119     593

  Fragments arranged by size: 

                1119     593     154
 
FspAI rTGC'GCAy

Cuts at:      0    1273    1866 
   Size:       1273     593
 
HaeII r_GCGC'y

Cuts at:      0     672    1866 
   Size:        672    1194
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     739     771     901     933    1018    1050    1700    1732 
   Size:        739      32     130      32      85      32     650      32

Cuts at:   1732    1866 
   Size:        134

  Fragments arranged by size: 

                 739     650     134     130      85      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     295    1866 
   Size:        295    1571
 
KasI G'GCGC_C

Cuts at:      0     668    1866 
   Size:        668    1198
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1310    1866 
   Size:       1310     556
 
MscI TGG'CCA

Cuts at:      0     560    1866 
   Size:        560    1306
 
MslI CAynn'nnrTG

Cuts at:      0    1813    1866 
   Size:       1813      53
 
MspA1I CmG'CkG

Cuts at:      0      24     117     760     768    1196    1742    1866 
   Size:         24      93     643       8     428     546     124

  Fragments arranged by size: 

                 643     546     428     124      93      24       8
 
NarI GG'CG_CC

Cuts at:      0     669    1866 
   Size:        669    1197
 
NspI r_CATG'y

Cuts at:      0      79     308     358    1122    1644    1816    1866 
   Size:         79     229      50     764     522     172      50

  Fragments arranged by size: 

                 764     522     229     172      79      50      50
 
PasI CC'CwG_GG

Cuts at:      0    1235    1866 
   Size:       1235     631
 
PciI A'CATG_T

Cuts at:      0    1640    1812    1866 
   Size:       1640     172      54

  Fragments arranged by size: 

                1640     172      54
 
PfoI T'CCnGG_A

Cuts at:      0    1064    1866 
   Size:       1064     802
 
PpuMI rG'GwC_Cy

Cuts at:      0     496     980    1200    1708    1866 
   Size:        496     484     220     508     158

  Fragments arranged by size: 

                 508     496     484     220     158
 
PstI C_TGCA'G

Cuts at:      0    1571    1866 
   Size:       1571     295
 
PvuII CAG'CTG

Cuts at:      0      24     760     768    1866 
   Size:         24     736       8    1098

  Fragments arranged by size: 

                1098     736      24       8
 
SacI G_AGCT'C

Cuts at:      0    1064    1866 
   Size:       1064     802
 
SanDI GG'GwC_CC

Cuts at:      0     496    1708    1866 
   Size:        496    1212     158

  Fragments arranged by size: 

                1212     496     158
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1717    1866 
   Size:       1717     149
 
SbfI CC_TGCA'GG

Cuts at:      0    1571    1866 
   Size:       1571     295
 
SexAI A'CCwGG_T

Cuts at:      0    1294    1866 
   Size:       1294     572
 
SfcI C'TryA_G

Cuts at:      0     454     512     575    1567    1866 
   Size:        454      58      63     992     299

  Fragments arranged by size: 

                 992     454     299      63      58
 
SfoI GGC'GCC

Cuts at:      0     670    1866 
   Size:        670    1196
 
SmlI C'TyrA_G

Cuts at:      0     287    1866 
   Size:        287    1579
 
StuI AGG'CCT

Cuts at:      0     208    1866 
   Size:        208    1658
 
StyI C'CwwG_G

Cuts at:      0     725    1126    1866 
   Size:        725     401     740

  Fragments arranged by size: 

                 740     725     401
 
TatI w'GTAC_w

Cuts at:      0     257    1866 
   Size:        257    1609
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     160     175     192     207     423     455     677     709 
   Size:        160      15      17      15     216      32     222      32

Cuts at:    709     799     831    1153    1185    1802    1834    1866 
   Size:         90      32     322      32     617      32      32

  Fragments arranged by size: 

                 617     322     222     216     160      90      32      32
                  32      32      32      32      17      15      15
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     138    1866 
   Size:        138    1728
 
XhoI C'TCGA_G

Cuts at:      0     287    1866 
   Size:        287    1579
 

 Enzymes that do cut:

     AarI     AclI     AcuI   AflIII     AhdI     AleI    AlwNI    ApaLI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI    BbvCI    BciVI
     BclI     BglI    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BstAPI   BstEII    BstYI     BtgI    BtgZI
     BtsI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FspI    FspAI    HaeII
    Hin4I  HindIII     KasI     MmeI     MscI     MslI   MspA1I     NarI
     NspI     PasI     PciI     PfoI    PpuMI     PstI    PvuII     SacI
    SanDI     SapI     SbfI    SexAI     SfcI     SfoI     SmlI     StuI
     StyI     TatI     TstI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AlfI     AloI
     ApaI     AscI     AseI    AsiSI    AvrII     BaeI     BbsI     BcgI
    BfrBI     BlpI     BmtI    BpuEI     BsaI    BsaAI    BsiWI    BsrGI
   BssHII    BssSI    BstBI    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
     DraI    EcoRI     FseI   HincII     HpaI     KpnI     MfeI     MluI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI     ScaI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII  Tth111I     XbaI     XmaI     XmnI     ZraI
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