Restriction map for pF1KE1963
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AgeI        AhdI        AlwI        AlwNI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvrII       BamHI       BbvCI       
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsaWI       BseAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstXI       BstYI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraIII      Eam1105I    EcoRI       EcoRV       EcoT22I     
FbaI        HincII      HindII      HindIII     HpaI        
KpnI        MamI        MfeI        MflI        MroI        
MseI        MunI        NcoI        NdeI        NheI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PflMI       PinAI       PleI        PmeI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SbfI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tth111I     Van91I      XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AatII         901  GACGTC               GACGT^C
AflIII       1028  ACGCGT               A^C[GA][CT]GT
Alw44I       1008  GTGCAC               G^TGCAC
AocI         1321  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       857  AGCGCT               AGC^GCT
ApaLI        1008  GTGCAC               G^TGCAC
AviII         545  TGCGCA               TGC^GCA
BbeI           91  GGCGCC               GGCGC^C
BbrPI        1084  CACGTG               CAC^GTG
BbsI          877  GAAGAC               GAAGAC(2/6)
BfaI          418  CTAG                 C^TAG
BpuAI         877  GAAGAC               GAAGAC(2/6)
BsaAI        1084  CACGTG               [CT]AC^GT[GA]
BsaI          421  GAGACC               GGTCTC(1/5)
BspMI         329  ACCTGC               ACCTGC(4/8)
BsrBI         223  GAGCGG               CCGCTC(-3/-3)
BsrFI         952  GCCGGC               [GA]^CCGG[CT]
BssSI        1069  CACGAG               CACGAG(-5/-1)
BstEII        618  GGTCACC              G^GT[ACGT]ACC
BstPI         618  GGTCACC              G^GT[ACGT]ACC
Bsu36I       1321  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        952  GCCGGC               [GA]^CCGG[CT]
DpnII         705  GATC                 ^GATC
DrdI         1356  GACGTACCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          334  CTCTTC               CTCTTC(1/4)
Eco47III      857  AGCGCT               AGC^GCT
Eco81I       1321  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       618  GGTCACC              G^GT[ACGT]ACC
FseI          951  GGCCGGCC             GGCCGG^CC
FspI          545  TGCGCA               TGC^GCA
KasI           91  GGCGCC               G^GCGCC
Ksp632I       334  CTCTTC               CTCTTC(1/4)
MaeI          418  CTAG                 C^TAG
MboI          705  GATC                 ^GATC
MluI         1028  ACGCGT               A^CGCGT
MslI         1476  CATCGACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          952  GCCGGC               GCC^GGC
NarI           91  GGCGCC               GG^CGCC
NdeII         705  GATC                 ^GATC
NgoMIV        952  GCCGGC               G^CCGGC
NotI          247  GCGGCCGC             GC^GGCCGC
PaeR7I        481  CTCGAG               C^TCGAG
PmaCI        1084  CACGTG               CAC^GTG
PmlI         1084  CACGTG               CAC^GTG
PshAI         601  GACAAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         1273  GAGCTC               GAGCT^C
SapI          333  GCTCTTC              GCTCTTC(1/4)
Sau3AI        705  GATC                 ^GATC
ScaI          467  AGTACT               AGT^ACT
SfiI          981  GGCCAGCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI           91  GGCGCC               GGC^GCC
SmlI          481  CTCGAG               C^T[CT][GA]AG
Tsp509I      1167  AATT                 ^AATT
XbaI          417  TCTAGA               T^CTAGA
XcmI         1475  CCATCGACGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          481  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI          784  GTCTAC               GT^[AC][GT]AC
             1369  GTCTAC              
BalI          980  TGGCCA               TGG^CCA
             1472  TGGCCA              
BanI           91  GGCGCC               G^G[CT][GA]CC
             1246  GGTGCC              
BcgI          759  CGAGGAGATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              256  GCAGTCCCCTCG        
BsgI         1279  CTGCAC               GTGCAG(16/14)
             1336  GTGCAG              
BsmBI         903  CGTCTC               CGTCTC(1/5)
              391  GAGACG              
EagI          248  CGGCCG               C^GGCCG
             1331  CGGCCG              
EclXI         248  CGGCCG               C^GGCCG
             1331  CGGCCG              
Eco52I        248  CGGCCG               C^GGCCG
             1331  CGGCCG              
EcoNI         201  CCTCATCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1287  CCTGTCTGAGG         
HinfI         735  GATTC                G^A[ACGT]TC
             1305  GATTC               
KspI          175  CCGCGG               CCGC^GG
              554  CCGCGG              
MaeIII        619  GTCAC                ^GT[ACGT]AC
             1394  GTGAC               
MboII         877  GAAGA                GAAGA(8/7)
              335  TCTTC               
MluNI         980  TGGCCA               TGG^CCA
             1472  TGGCCA              
MscI          980  TGGCCA               TGG^CCA
             1472  TGGCCA              
PstI          134  CTGCAG               CTGCA^G
              254  CTGCAG              
SacII         175  CCGCGG               CCGC^GG
              554  CCGCGG              
SexAI         614  ACCTGGT              A^CC[AT]GGT
              668  ACCAGGT             
SfaNI         148  GCATC                GCATC(5/9)
             1430  GCATC               
SfcI          134  CTGCAG               C^T[GA][CT]AG
              254  CTGCAG              
StuI          839  AGGCCT               AGG^CCT
             1238  AGGCCT              
TfiI          735  GATTC                G^A[AT]TC
             1305  GATTC               
Tsp45I        619  GTCAC                ^GT[GC]AC
             1394  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
ApaI        AspHI       AvaI        AvaII       BanII       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       HphI        ItaI        MaeII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NlaIII      NlaIV       PpuMI       
PvuII       RsaI        Sau96I      ScrFI       SmaI        
StyI        TaqI        TseI        TspRI       TthHB8I     
XmaCI       XmaI        
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