Restriction map for pF1KB8518
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvrII       
BalI        BbeI        BbrPI       BcgI        BclI        
BfrI        BlnI        BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BspEI       BspHI       
BspLU11I    BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstZ17I     CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoRV       FbaI        FseI        HaeII       
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        MvnI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    StuI        
SwaI        Tth111I     XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII        1398  GACGTC               GACGT^C
AcyI         1398  GACGTC               G[GA]^CG[CT]C
AocI         1030  CCTGAGG              CC^T[ACGT]AGG
ApaI         2077  GGGCCC               GGGCC^C
AvaI          586  CTCGGG               C^[CT]CG[GA]G
AviII        1216  TGCGCA               TGC^GCA
BamHI         638  GGATCC               G^GATCC
BanI         1779  GGCACC               G^G[CT][GA]CC
BbvCI        1108  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1707  GTATCC               GTATCC(6/5)
BglII        2381  AGATCT               A^GATCT
BsaAI        2440  TACGTG               [CT]AC^GT[GA]
BsaHI        1398  GACGTC               G[GA]^CG[CT]C
BsaI         2064  GGTCTC               GGTCTC(1/5)
BsoBI         586  CTCGGG               C^[CT]CG[GA]G
BspDI         563  ATCGAT               AT^CGAT
BspMI        2121  GCAGGT               ACCTGC(4/8)
BsrBI        1672  CCGCTC               CCGCTC(-3/-3)
BsrDI        2112  CATTGC               GCAATG(2/0)
BsrFI        1097  GCCGGC               [GA]^CCGG[CT]
BstEII       1981  GGTGACC              G^GT[ACGT]ACC
BstPI        1981  GGTGACC              G^GT[ACGT]ACC
BstXI        2117  CCAGGCAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1030  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1097  GCCGGC               [GA]^CCGG[CT]
ClaI          563  ATCGAT               AT^CGAT
Eco81I       1030  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1981  GGTGACC              G^GT[ACGT]ACC
EcoRI        2561  GAATTC               G^AATTC
FauI          645  GCGGG                CCCGC(4/6)
FspI         1216  TGCGCA               TGC^GCA
Hin1I        1398  GACGTC               G[GA]^CG[CT]C
HincII         13  GTCAAC               GT[CT]^[GA]AC
HindII         13  GTCAAC               GT[CT]^[GA]AC
HindIII      1306  AAGCTT               A^AGCTT
NaeI         1097  GCCGGC               GCC^GGC
NgoMIV       1097  GCCGGC               G^CCGGC
PflMI         725  CCATCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1325  GGGACCC              [GA]G^G[AT]CC[CT]
SapI         1209  GCTCTTC              GCTCTTC(1/4)
SphI         1316  GCATGC               GCATG^C
SspI         1515  AATATT               AAT^ATT
TaqI          564  TCGA                 T^CGA
TthHB8I       564  TCGA                 T^CGA
Van91I        725  CCATCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1356  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

BbsI          268  GAAGAC               GAAGAC(2/6)
             1245  GAAGAC              
BcnI          218  CCCGG                CC^[GC]GG
              231  CCGGG               
BglI         1882  GCCCTCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2192  GCCTGGTGGGC         
BlpI         1695  GCTCAGC              GC^T[ACGT]AGC
             2098  GCTGAGC             
Bpu1102I     1695  GCTCAGC              GC^T[ACGT]AGC
             2098  GCTGAGC             
BpuAI         268  GAAGAC               GAAGAC(2/6)
             1245  GAAGAC              
BsmI         1297  GAATGC               GAATGC(1/-1)
              710  GCATTC              
Bsp1407I      697  TGTACA               T^GTACA
             2492  TGTACA              
BsrGI         697  TGTACA               T^GTACA
             2492  TGTACA              
BstAPI        284  GCATTTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              988  GCAAAAGATGC         
CelII        1695  GCTCAGC              GC^T[ACGT]AGC
             2098  GCTGAGC             
CfoI          950  GCGC                 GCG^C
             1217  GCGC                
DraII        1325  GGGACCC              [GA]G^G[ACGT]CC[CT]
             2077  GGGCCCC             
DraIII       2017  CACACCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2019  CACCGTGTG           
DsaI         1331  CCACGG               C^C[GA][CT]GG
             2483  CCGTGG              
EaeI          299  CGGCCA               [CT]^GGCC[GA]
             1099  CGGCCA              
EcoO109I     1325  GGGACCC              [GA]G^G[ACGT]CC[CT]
             2077  GGGCCCC             
EcoT14I       884  CCAAGG               C^C[AT][AT]GG
             1711  CCAAGG              
HgaI         2284  GACGC                GACGC(5/10)
             2505  GCGTC               
HhaI          950  GCGC                 GCG^C
             1217  GCGC                
HinP1I        950  GCGC                 G^CGC
             1217  GCGC                
MseI          203  TTAA                 T^TAA
              406  TTAA                
NciI          218  CCCGG                CC^[GC]GG
              231  CCGGG               
ScaI          877  AGTACT               AGT^ACT
             1187  AGTACT              
SmlI          522  CTTGAG               C^T[CT][GA]AG
              801  CTCAAG              
SspBI         697  TGTACA               T^GTACA
             2492  TGTACA              
StyI          884  CCAAGG               C^C[AT][AT]GG
             1711  CCAAGG              
Tru9I         203  TTAA                 T^TAA
              406  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BanII       
BbvI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       EarI        EcoRII      EcoT22I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       XcmI        XhoII       
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