Restriction map for pF1KE2398
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AflIII      AhdI        Alw44I      AlwNI       AocI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvrII       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaI        
BseRI       BsiWI       BsmAI       BsmBI       BsoBI       
BspDI       BspLU11I    BsrBI       BsrDI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
DraIII      DrdI        Eam1105I    EarI        Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        HindIII     HpaI        KasI        KpnI        
Ksp632I     MaeIII      MamI        MfeI        MluI        
MunI        NarI        NcoI        NdeI        NheI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
SalI        SapI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        SspI        StuI        SwaI        Tsp45I      
Tsp509I     TspRI       Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII         766  GACGTC               GACGT^C
AgeI          533  ACCGGT               A^CCGGT
Aor51HI       506  AGCGCT               AGC^GCT
ApaI          734  GGGCCC               GGGCC^C
AviII           8  TGCGCA               TGC^GCA
BamHI         167  GGATCC               G^GATCC
BbsI          207  GAAGAC               GAAGAC(2/6)
BclI          329  TGATCA               T^GATCA
BpuAI         207  GAAGAC               GAAGAC(2/6)
BsgI          338  GTGCAG               GTGCAG(16/14)
BsmI          215  GAATGC               GAATGC(1/-1)
Bsp1407I      518  TGTACA               T^GTACA
BspHI         326  TCATGA               T^CATGA
BsrGI         518  TGTACA               T^GTACA
BstAPI        504  GCAGCGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI          286  CGGACCG              CG^G[AT]CCG
DraI          393  TTTAAA               TTT^AAA
DsaI          743  CCGCGG               C^C[GA][CT]GG
Eco47III      506  AGCGCT               AGC^GCT
EcoT14I       414  CCAAGG               C^C[AT][AT]GG
FbaI          329  TGATCA               T^GATCA
FspI            8  TGCGCA               TGC^GCA
KspI          743  CCGCGG               CCGC^GG
MseI          394  TTAA                 T^TAA
NotI          745  GCGGCCGC             GC^GGCCGC
PinAI         533  ACCGGT               A^CCGGT
PstI          664  CTGCAG               CTGCA^G
RcaI          326  TCATGA               T^CATGA
RsrII         286  CGGACCG              CG^G[AT]CCG
SacI         1004  GAGCTC               GAGCT^C
SacII         743  CCGCGG               CCGC^GG
SbfI          663  CCTGCAGG             CCTGCA^GG
ScaI          971  AGTACT               AGT^ACT
SfcI          664  CTGCAG               C^T[GA][CT]AG
SmlI          271  CTCAAG               C^T[CT][GA]AG
Sse8387I      663  CCTGCAGG             CCTGCA^GG
SspBI         518  TGTACA               T^GTACA
StyI          414  CCAAGG               C^C[AT][AT]GG
TaqI         1002  TCGA                 T^CGA
TfiI          483  GAATC                G^A[AT]TC
Tru9I         394  TTAA                 T^TAA
TthHB8I      1002  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccIII        554  TCCGGA               T^CCGGA
              857  TCCGGA              
AspHI         175  GAGCAC               G[AT]GC[AT]^C
             1004  GAGCTC              
BfaI          122  CTAG                 C^TAG
              430  CTAG                
BglI           62  GCCGAGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              881  GCCTGGCCGGC         
BpmI          172  CTGGAG               CTGGAG(16/14)
              300  CTGGAG              
BseAI         554  TCCGGA               T^CCGGA
              857  TCCGGA              
BsiEI         746  CGGCCG               CG[GA][CT]^CG
              828  CGGCCG              
BsiHKAI       175  GAGCAC               G[AT]GC[AT]^C
             1004  GAGCTC              
BsmFI          56  GGGAC                GGGAC(10/14)
              705  GTCCC               
BspEI         554  TCCGGA               T^CCGGA
              857  TCCGGA              
BspMI         113  ACCTGC               ACCTGC(4/8)
              672  GCAGGT              
BsrI          246  CCAGT                ACTGG(1/-1)
              633  CCAGT               
BstYI         167  GGATCC               [GA]^GATC[CT]
              234  AGATCC              
DdeI          717  CTCAG                C^T[ACGT]AG
              791  CTCAG               
EagI          746  CGGCCG               C^GGCCG
              828  CGGCCG              
EclXI         746  CGGCCG               C^GGCCG
              828  CGGCCG              
Eco52I        746  CGGCCG               C^GGCCG
              828  CGGCCG              
HgaI           85  GACGC                GACGC(5/10)
              317  GCGTC               
HincII        319  GTCAAC               GT[CT]^[GA]AC
              676  GTCAAC              
HindII        319  GTCAAC               GT[CT]^[GA]AC
              676  GTCAAC              
MaeI          122  CTAG                 C^TAG
              430  CTAG                
MboII         207  GAAGA                GAAGA(8/7)
              965  TCTTC               
MflI          167  GGATCC               [GA]^GATC[CT]
              234  AGATCC              
MroI          554  TCCGGA               T^CCGGA
              857  TCCGGA              
MslI          138  CACCCACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              327  CATGATCATG          
NaeI          830  GCCGGC               GCC^GGC
              886  GCCGGC              
NgoMIV        830  GCCGGC               G^CCGGC
              886  GCCGGC              
PflMI          30  CCAGGCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              941  CCATTTCTTGG         
PleI          199  GACTC                GAGTC(4/5)
              228  GAGTC               
Van91I         30  CCAGGCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              941  CCATTTCTTGG         
XcmI          752  CCACGACCCCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              983  CCAAGCGCCAGCTGG     
XhoII         167  GGATCC               [GA]^GATC[CT]
              234  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AvaII       BalI        BanII       BbvI        
BcnI        BmyI        BsaHI       BsaJI       BsaWI       
BsiYI       BslI        Bsp1286I    BsrFI       BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DpnII       DraII       EaeI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MboI        MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TseI        
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