Restriction map for pF1KE5297
 (Circular) (Six-base) MAPSORT of: pF1KE5297.seq  Check: 5832  from: 1  to: 6524



pF1KE5297  6524 bp

 With 182 enzymes: SgfI * 

                        December 2, 2014 13:35  ..

AccI GT'mk_AC

Cuts at:    779    3562     779 
   Size:       2783    3741
 
Acc65I G'GTAC_C

Cuts at:   2510    3540    2510 
   Size:       1030    5494
 
AclI AA'CG_TT

Cuts at:    835    6364     835 
   Size:       5529     995
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    463     675     809     858    1279    1635    4349    4781    5528 
   Size:        212     134      49     421     356    2714     432     747

Cuts at:   5528    5796     463 
   Size:        268    1191

  Fragments arranged by size: 

                2714    1191     747     432     421     356     268     212
                 134      49
 
AfeI AGC'GCT

Cuts at:   2333    2333 
   Size:       6524
 
AflIII A'CryG_T

Cuts at:    743     782    2099    2345    2364    3876    4980    5957     743 
   Size:         39    1317     246      19    1512    1104     977    1310

  Fragments arranged by size: 

                1512    1317    1310    1104     977     246      39      19
            
 
AgeI A'CCGG_T

Cuts at:   4912    4912 
   Size:       6524
 
AleI CACnn'nnGTG

Cuts at:    742    1633    2104    2617    2951     742 
   Size:        891     471     513     334    4315

  Fragments arranged by size: 

                4315     891     513     471     334
 
AlwNI CAG_nnn'CTG

Cuts at:    206     537    1257    1617    2967    3021    3829    5396     206 
   Size:        331     720     360    1350      54     808    1567    1334

  Fragments arranged by size: 

                1567    1350    1334     808     720     360     331      54
            
 
ApaI G_GGCC'C

Cuts at:   3212    3212 
   Size:       6524
 
ApaLI G'TGCA_C

Cuts at:   1759    2742    5294    1759 
   Size:        983    2552    2989

  Fragments arranged by size: 

                2989    2552     983
 
ApoI r'AATT_y

Cuts at:    574    2683    2698    3528    5877    6133     574 
   Size:       2109      15     830    2349     256     965

  Fragments arranged by size: 

                2349    2109     965     830     256      15
 
AseI AT'TA_AT

Cuts at:     20    1088      20 
   Size:       1068    5456
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6524
 
AvaI C'yCGr_G

Cuts at:   2066    2393    2822    2937    3544    2066 
   Size:        327     429     115     607    5046

  Fragments arranged by size: 

                5046     607     429     327     115
 
BamHI G'GATC_C

Cuts at:   1142    3549    1142 
   Size:       2407    4117
 
BanI G'GyrC_C

Cuts at:    286     658    1324    1737    2389    2510    3439    3540    3954 
   Size:        372     666     413     652     121     929     101     414

Cuts at:   3954    4202    4237     286 
   Size:        248      35    2573

  Fragments arranged by size: 

                2573     929     666     652     414     413     372     248
                 121     101      35
 
BanII G_rGCy'C

Cuts at:   1705    2431    3058    3212    3538    4568    1705 
   Size:        726     627     154     326    1030    3661

  Fragments arranged by size: 

                3661    1030     726     627     326     154
 
BbeI G_GCGC'C

Cuts at:    662    1328    1741    3443    3958    4206     662 
   Size:        666     413    1702     515     248    2980

  Fragments arranged by size: 

                2980    1702     666     515     413     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    259     798    1641    1904    2541    3084     259 
   Size:        539     843     263     637     543    3699

  Fragments arranged by size: 

                3699     843     637     543     539     263
 
BbvCI CC'TCA_GC

Cuts at:   1021    1597    3453    1021 
   Size:        576    1856    4092

  Fragments arranged by size: 

                4092    1856     576
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2403    2437    2403 
   Size:         34    6490
 
BciVI GTATCCnnnnn_n'

Cuts at:   1759    4422    5183    6524    1759 
   Size:       2663     761    1341    1759

  Fragments arranged by size: 

                2663    1759    1341     761
 
BclI T'GATC_A

Cuts at:    617    1928    4044     617 
   Size:       1311    2116    3097

  Fragments arranged by size: 

                3097    2116    1311
 
BglI GCCn_nnn'nGGC

Cuts at:    556     556 
   Size:       6524
 
BglII A'GATC_T

Cuts at:   1472    4039    6076    1472 
   Size:       2567    2037    1920

  Fragments arranged by size: 

                2567    2037    1920
 
BlpI GC'TnA_GC

Cuts at:   1525    2203    2878    3636    1525 
   Size:        678     675     758    4413

  Fragments arranged by size: 

                4413     758     678     675
 
Bme1580I G_kGCm'C

Cuts at:   1763    2392    2746    3212    4149    4242    5298    1763 
   Size:        629     354     466     937      93    1056    2989

  Fragments arranged by size: 

                2989    1056     937     629     466     354      93
 
BmgBI CAC'GTC

Cuts at:    999    2102     999 
   Size:       1103    5421
 
BmrI ACTGGGnnnn_n'

Cuts at:   3736    3817    4152    6296    3736 
   Size:         81     335    2144    3964

  Fragments arranged by size: 

                3964    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2457    4891    2457 
   Size:       2434    4090
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    180     202     694    2275    2674    3058     180 
   Size:         22     492    1581     399     384    3646

  Fragments arranged by size: 

                3646    1581     492     399     384      22
 
Bpu10I CC'TnA_GC

Cuts at:   1021    1597    3453    1021 
   Size:        576    1856    4092

  Fragments arranged by size: 

                4092    1856     576
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3700    4579    5071    5369    3700 
   Size:        879     492     298    4855

  Fragments arranged by size: 

                4855     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    821    2445    6228     821 
   Size:       1624    3783    1117

  Fragments arranged by size: 

                3783    1624    1117
 
BsaAI yAC'GTr

Cuts at:   1521    2367    3877    1521 
   Size:        846    1510    4168

  Fragments arranged by size: 

                4168    1510     846
 
BsaBI GATnn'nnATC

Cuts at:      6    4038    6075       6 
   Size:       4032    2037     455

  Fragments arranged by size: 

                4032    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    659    1325    1738    2666    3440    3955    4203    4905    6401 
   Size:        666     413     928     774     515     248     702    1496

Cuts at:   6401     659 
   Size:        782

  Fragments arranged by size: 

                1496     928     782     774     702     666     515     413
                 248
 
BsaWI w'CCGG_w

Cuts at:   2195    3356    3713    4234    4912    5186    5333    5744    2195 
   Size:       1161     357     521     678     274     147     411    2975

  Fragments arranged by size: 

                2975    1161     678     521     411     357     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    178     208    1786    1816    2272    2302     178 
   Size:         30    1578      30     456      30    4400

  Fragments arranged by size: 

                4400    1578     456      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    466     568    1210    1585    2281    2368    3247    3250    3430 
   Size:        102     642     375     696      87     879       3     180

Cuts at:   3430     466 
   Size:       3560

  Fragments arranged by size: 

                3560     879     696     642     375     180     102      87
                   3
 
BseYI C'CCAG_C

Cuts at:    190     654    2561    2870    2960    3949    4707    5284    5780 
   Size:        464    1907     309      90     989     758     577     496

Cuts at:   5780     190 
   Size:        934

  Fragments arranged by size: 

                1907     989     934     758     577     496     464     309
                  90
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    268     748    1501    2742    2975    3253     268 
   Size:        480     753    1241     233     278    3539

  Fragments arranged by size: 

                3539    1241     753     480     278     233
 
BsiEI CG_ry'CG

Cuts at:    104    4066    4112    5320     104 
   Size:       3962      46    1208    1308

  Fragments arranged by size: 

                3962    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1526    1763    2746    3538    4316    4506    5298    1526 
   Size:        237     983     792     778     190     792    2752

  Fragments arranged by size: 

                2752     983     792     792     778     237     190
 
BsmI GAATG_Cn'

Cuts at:    804    1682    3426     804 
   Size:        878    1744    3902

  Fragments arranged by size: 

                3902    1744     878
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2821    2821 
   Size:       6524
 
Bsp1286I G_dGCh'C

Cuts at:   1526    1705    1763    2392    2431    2746    3058    3212    3538 
   Size:        179      58     629      39     315     312     154     326

Cuts at:   3538    4149    4242    4316    4506    4568    5298    1526 
   Size:        611      93      74     190      62     730    2752

  Fragments arranged by size: 

                2752     730     629     611     326     315     312     190
                 179     154      93      74      62      58      39
 
BspEI T'CCGG_A

Cuts at:   5744    5744 
   Size:       6524
 
BspHI T'CATG_A

Cuts at:   5700    5700 
   Size:       6524
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    707    1523    3574    3788    4090    4471     707 
   Size:        816    2051     214     302     381    2760

  Fragments arranged by size: 

                2760    2051     816     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1004    4816    4870    6354    6519      50 
   Size:        954    3812      54    1484     165      55

  Fragments arranged by size: 

                3812    1484     954     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2648    4436    2648 
   Size:       1788    4736
 
BsrFI r'CCGG_y

Cuts at:    702    1344    2152    3171    3380    4522    4703    4912     702 
   Size:        642     808    1019     209    1142     181     209    2314

  Fragments arranged by size: 

                2314    1142    1019     808     642     209     209     181
            
 
BsrGI T'GTAC_A

Cuts at:    746    1826     746 
   Size:       1080    5444
 
BssSI C'ACGA_G

Cuts at:   4795    5153    4795 
   Size:        358    6166
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2127    3901    2127 
   Size:       1774    4750
 
BstBI TT'CG_AA

Cuts at:   4885    4885 
   Size:       6524
 
BstEII G'GTnAC_C

Cuts at:   2625    2625 
   Size:       6524
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1059    1059 
   Size:       6524
 
BstYI r'GATC_y

Cuts at:      1     878    1142    1253    1472    1875    2162    3549    4016 
   Size:        877     264     111     219     403     287    1387     467

Cuts at:   4016    4039    4374    4620    5632    5718    5730    6076       1 
   Size:         23     335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1387    1012     877     467     449     403     346     335
                 287     264     246     219     111      86      23      12
            
 
Bsu36I CC'TnA_GG

Cuts at:   2465    2465 
   Size:       6524
 
BtgI C'CryG_G

Cuts at:    107     230     942    1328    1535    1677    2073    2312    2594 
   Size:        123     712     386     207     142     396     239     282

Cuts at:   2594    2630    2660     107 
   Size:         36      30    3971

  Fragments arranged by size: 

                3971     712     396     386     282     239     207     142
                 123      36      30
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4474    4655    4818    4474 
   Size:        181     163    6180

  Fragments arranged by size: 

                6180     181     163
 
BtsI GCAGTG_nn'

Cuts at:    864    1621    2725    3767     864 
   Size:        757    1104    1042    3621

  Fragments arranged by size: 

                3621    1104    1042     757
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    212     247     212 
   Size:         35    6489
 
DraI TTT'AAA

Cuts at:    770    1137    3523     770 
   Size:        367    2386    3771

  Fragments arranged by size: 

                3771    2386     367
 
DraIII CAC_nnn'GTG

Cuts at:   2370    2370 
   Size:       6524
 
DrdI GACnn_nn'nnGTC

Cuts at:   1378    2779    4230    5088    1378 
   Size:       1401    1451     858    2814

  Fragments arranged by size: 

                2814    1451    1401     858
 
EaeI y'GGCC_r

Cuts at:   1366    1637    2150    3173    4109    4283    4674    4701    6046 
   Size:        271     513    1023     936     174     391      27    1345

Cuts at:   6046    1366 
   Size:       1844

  Fragments arranged by size: 

                1844    1345    1023     936     513     391     271     174
                  27
 
EagI C'GGCC_G

Cuts at:   4109    4109 
   Size:       6524
 
EarI CTCTTCn'nnn_

Cuts at:   4547    4757    4547 
   Size:        210    6314
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1171    1501    3367    4732    5042    5188    6334    1171 
   Size:        330    1866    1365     310     146    1146    1361

  Fragments arranged by size: 

                1866    1365    1361    1146     330     310     146
 
EcoICRI GAG'CTC

Cuts at:   3536    3536 
   Size:       6524
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    180     202     463     675     694     809     858    1279    1635 
   Size:         22     261     212      19     115      49     421     356

Cuts at:   1635    2275    2674    3058    4349    4781    5528    5796     180 
   Size:        640     399     384    1291     432     747     268     908

  Fragments arranged by size: 

                1291     908     747     640     432     421     399     384
                 356     268     261     212     115      49      22      19
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1150    1150 
   Size:       6524
 
EcoO109I rG'GnC_Cy

Cuts at:    366    2244    2819    3123    3209    3663     366 
   Size:       1878     575     304      86     454    3227

  Fragments arranged by size: 

                3227    1878     575     454     304      86
 
EcoRI G'AATT_C

Cuts at:    574    2683    3528     574 
   Size:       2109     845    3570

  Fragments arranged by size: 

                3570    2109     845
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    660    4543     660 
   Size:       3883    2641
 
FspI TGC'GCA

Cuts at:   4305    4305 
   Size:       6524
 
HaeII r_GCGC'y

Cuts at:    662    1328    1741    2335    3443    3958    4206    5228    6207 
   Size:        666     413     594    1108     515     248    1022     979

Cuts at:   6207     662 
   Size:        979

  Fragments arranged by size: 

                1108    1022     979     979     666     594     515     413
                 248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    418     450    2069    2101    4366    4396    4398    4428     418 
   Size:         32    1619      32    2265      30       2      30    2514

  Fragments arranged by size: 

                2514    2265    1619      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    839    3563     839 
   Size:       2724    3800
 
HindIII A'AGCT_T

Cuts at:   1750    1750 
   Size:       6524
 
KasI G'GCGC_C

Cuts at:    658    1324    1737    3439    3954    4202     658 
   Size:        666     413    1702     515     248    2980

  Fragments arranged by size: 

                2980    1702     666     515     413     248
 
KpnI G_GTAC'C

Cuts at:   2514    3544    2514 
   Size:       1030    5494
 
MluI A'CGCG_T

Cuts at:   5957    5957 
   Size:       6524
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2468    5195    5379    2468 
   Size:       2727     184    3613

  Fragments arranged by size: 

                3613    2727     184
 
MscI TGG'CCA

Cuts at:   1368    1639    4285    1368 
   Size:        271    2646    3607

  Fragments arranged by size: 

                3607    2646     271
 
MslI CAynn'nnrTG

Cuts at:    742    1633    2104    2599    2617    2887    2951    4513    4640 
   Size:        891     471     495      18     270      64    1562     127

Cuts at:   4640     742 
   Size:       2626

  Fragments arranged by size: 

                2626    1562     891     495     471     270     127      64
                  18
 
MspA1I CmG'CkG

Cuts at:    206     732     944    2505    2561    2617    2662    2874    3129 
   Size:        526     212    1561      56      56      45     212     255

Cuts at:   3129    3635    3949    4309    5322    5567     206 
   Size:        506     314     360    1013     245    1163

  Fragments arranged by size: 

                1561    1163    1013     526     506     360     314     255
                 245     212     212      56      56      45
 
NaeI GCC'GGC

Cuts at:   3173    3382    4705    3173 
   Size:        209    1323    4992

  Fragments arranged by size: 

                4992    1323     209
 
NarI GG'CG_CC

Cuts at:    659    1325    1738    3440    3955    4203     659 
   Size:        666     413    1702     515     248    2980

  Fragments arranged by size: 

                2980    1702     666     515     413     248
 
NcoI C'CATG_G

Cuts at:    107    1535    1677    2312    2594     107 
   Size:       1428     142     635     282    4037

  Fragments arranged by size: 

                4037    1428     635     282     142
 
NgoMIV G'CCGG_C

Cuts at:   3171    3380    4703    3171 
   Size:        209    1323    4992

  Fragments arranged by size: 

                4992    1323     209
 
NspI r_CATG'y

Cuts at:    507     786    1448    2349    3577    4077    4984     507 
   Size:        279     662     901    1228     500     907    2047

  Fragments arranged by size: 

                2047    1228     907     901     662     500     279
 
PasI CC'CwG_GG

Cuts at:   1068    1068 
   Size:       6524
 
PciI A'CATG_T

Cuts at:    782    2345    4980     782 
   Size:       1563    2635    2326

  Fragments arranged by size: 

                2635    2326    1563
 
PflMI CCAn_nnn'nTGG

Cuts at:    537    2967     537 
   Size:       2430    4094
 
PfoI T'CCnGG_A

Cuts at:    671    2276    3179     671 
   Size:       1605     903    4016

  Fragments arranged by size: 

                4016    1605     903
 
PmeI GTTT'AAAC

Cuts at:   3523    3523 
   Size:       6524
 
PmlI CAC'GTG

Cuts at:   1521    2367    1521 
   Size:        846    5678
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5663    5695    5663 
   Size:         32    6492
 
PpuMI rG'GwC_Cy

Cuts at:    366    2244    2819    3123     366 
   Size:       1878     575     304    3767

  Fragments arranged by size: 

                3767    1878     575     304
 
PshAI GACnn'nnGTC

Cuts at:   3325    3325 
   Size:       6524
 
PspOMI G'GGCC_C

Cuts at:   3208    3208 
   Size:       6524
 
PspXI vC'TCGA_Gb

Cuts at:   2937    2937 
   Size:       6524
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   3142    3174    3142 
   Size:         32    6492
 
PstI C_TGCA'G

Cuts at:    413    2658    3017    3134    3292    3314    3571    4256     413 
   Size:       2245     359     117     158      22     257     685    2681

  Fragments arranged by size: 

                2681    2245     685     359     257     158     117      22
            
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6524
 
PvuII CAG'CTG

Cuts at:    732    2617    2874    3949    4309     732 
   Size:       1885     257    1075     360    2947

  Fragments arranged by size: 

                2947    1885    1075     360     257
 
RsrII CG'GwC_CG

Cuts at:   1158    4719    1158 
   Size:       3561    2963
 
SacI G_AGCT'C

Cuts at:   3538    3538 
   Size:       6524
 
SacII CC_GC'GG

Cuts at:    945    2663     945 
   Size:       1718    4806
 
SalI G'TCGA_C

Cuts at:   3561    3561 
   Size:       6524
 
SapI GCTCTTCn'nnn_

Cuts at:   4547    4757    4547 
   Size:        210    6314
 
SbfI CC_TGCA'GG

Cuts at:   3571    3571 
   Size:       6524
 
ScaI AGT'ACT

Cuts at:   6041    6041 
   Size:       6524
 
SexAI A'CCwGG_T

Cuts at:   3001    3001 
   Size:       6524
 
SfcI C'TryA_G

Cuts at:     33     409    2654    3013    3130    3288    3310    3567    4252 
   Size:        376    2245     359     117     158      22     257     685

Cuts at:   4252    5245    5436      33 
   Size:        993     191    1121

  Fragments arranged by size: 

                2245    1121     993     685     376     359     257     191
                 158     117      22
 
SfoI GGC'GCC

Cuts at:    660    1326    1739    3441    3956    4204     660 
   Size:        666     413    1702     515     248    2980

  Fragments arranged by size: 

                2980    1702     666     515     413     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6524
 
SgrAI Cr'CCGG_yG

Cuts at:   3380    3380 
   Size:       6524
 
SmaI CCC'GGG

Cuts at:   2068    2395    3546    2068 
   Size:        327    1151    5046

  Fragments arranged by size: 

                5046    1151     327
 
SmlI C'TyrA_G

Cuts at:   2937    3679    4594    5086    5348    2937 
   Size:        742     915     492     262    4113

  Fragments arranged by size: 

                4113     915     742     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6524
 
SphI G_CATG'C

Cuts at:   1448    3577    4077    1448 
   Size:       2129     500    3895

  Fragments arranged by size: 

                3895    2129     500
 
SspI AAT'ATT

Cuts at:    850     850 
   Size:       6524
 
StuI AGG'CCT

Cuts at:   1576    2117    2521    1576 
   Size:        541     404    5579

  Fragments arranged by size: 

                5579     541     404
 
StyI C'CwwG_G

Cuts at:    107     974    1535    1677    2312    2594    3658    4012     107 
   Size:        867     561     142     635     282    1064     354    2619

  Fragments arranged by size: 

                2619    1064     867     635     561     354     282     142
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    600    1175    1507    1681    2150    4107    4240    4699    4908 
   Size:        575     332     174     469    1957     133     459     209

Cuts at:   4908     600 
   Size:       2216

  Fragments arranged by size: 

                2216    1957     575     469     459     332     209     174
                 133
 
TatI w'GTAC_w

Cuts at:    419     746    1826    6039     419 
   Size:        327    1080    4213     904

  Fragments arranged by size: 

                4213    1080     904     327
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2725    2757    6041    6073    2725 
   Size:         32    3284      32    3176

  Fragments arranged by size: 

                3284    3176      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1111    4321    1111 
   Size:       3210    3314
 
XbaI T'CTAG_A

Cuts at:   3555    3555 
   Size:       6524
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    324     839     893    3195    4019     324 
   Size:        515      54    2302     824    2829

  Fragments arranged by size: 

                2829    2302     824     515      54
 
XhoI C'TCGA_G

Cuts at:   2937    2937 
   Size:       6524
 
XmaI C'CCGG_G

Cuts at:   2066    2393    3544    2066 
   Size:        327    1151    5046

  Fragments arranged by size: 

                5046    1151     327
 
XmnI GAAnn'nnTTC

Cuts at:   1081    1264    1081 
   Size:        183    6341
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AleI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AlfI     AloI     AscI    AvrII
     BaeI    BfrBI     BmtI    BsiWI   BssHII  BstZ17I     ClaI    EcoRV
     FseI    FspAI     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PsiI    SanDI     SfiI    SnaBI     SrfI     SwaI
     ZraI
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