Restriction map for pF1KE5297
 (Linear) (Six-base) MAPSORT of: KIEE5297.seq  Check: 5286  from: 1  to: 3411



KIEE5297.orf

 With 182 enzymes: SgfI * 

                        December 2, 2014 13:35  ..

AccI GT'mk_AC

Cuts at:      0     671    3411 
   Size:        671    2740
 
Acc65I G'GTAC_C

Cuts at:      0    2402    3411 
   Size:       2402    1009
 
AclI AA'CG_TT

Cuts at:      0     727    3411 
   Size:        727    2684
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     567     701     750    1171    1527    3411 
   Size:        355     212     134      49     421     356    1884

  Fragments arranged by size: 

                1884     421     356     355     212     134      49
 
AfeI AGC'GCT

Cuts at:      0    2225    3411 
   Size:       2225    1186
 
AflIII A'CryG_T

Cuts at:      0     635     674    1991    2237    2256    3411 
   Size:        635      39    1317     246      19    1155

  Fragments arranged by size: 

                1317    1155     635     246      39      19
 
AleI CACnn'nnGTG

Cuts at:      0     634    1525    1996    2509    2843    3411 
   Size:        634     891     471     513     334     568

  Fragments arranged by size: 

                 891     634     568     513     471     334
 
AlwNI CAG_nnn'CTG

Cuts at:      0      98     429    1149    1509    2859    2913    3411 
   Size:         98     331     720     360    1350      54     498

  Fragments arranged by size: 

                1350     720     498     360     331      98      54
 
ApaI G_GGCC'C

Cuts at:      0    3104    3411 
   Size:       3104     307
 
ApaLI G'TGCA_C

Cuts at:      0    1651    2634    3411 
   Size:       1651     983     777

  Fragments arranged by size: 

                1651     983     777
 
ApoI r'AATT_y

Cuts at:      0     466    2575    2590    3411 
   Size:        466    2109      15     821

  Fragments arranged by size: 

                2109     821     466      15
 
AseI AT'TA_AT

Cuts at:      0     980    3411 
   Size:        980    2431
 
AvaI C'yCGr_G

Cuts at:      0    1958    2285    2714    2829    3411 
   Size:       1958     327     429     115     582

  Fragments arranged by size: 

                1958     582     429     327     115
 
BamHI G'GATC_C

Cuts at:      0    1034    3411 
   Size:       1034    2377
 
BanI G'GyrC_C

Cuts at:      0     178     550    1216    1629    2281    2402    3331    3411 
   Size:        178     372     666     413     652     121     929      80

  Fragments arranged by size: 

                 929     666     652     413     372     178     121      80
            
 
BanII G_rGCy'C

Cuts at:      0    1597    2323    2950    3104    3411 
   Size:       1597     726     627     154     307

  Fragments arranged by size: 

                1597     726     627     307     154
 
BbeI G_GCGC'C

Cuts at:      0     554    1220    1633    3335    3411 
   Size:        554     666     413    1702      76

  Fragments arranged by size: 

                1702     666     554     413      76
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     151     690    1533    1796    2433    2976    3411 
   Size:        151     539     843     263     637     543     435

  Fragments arranged by size: 

                 843     637     543     539     435     263     151
 
BbvCI CC'TCA_GC

Cuts at:      0     913    1489    3345    3411 
   Size:        913     576    1856      66

  Fragments arranged by size: 

                1856     913     576      66
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2295    2329    3411 
   Size:       2295      34    1082

  Fragments arranged by size: 

                2295    1082      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1651    3411 
   Size:       1651    1760
 
BclI T'GATC_A

Cuts at:      0     509    1820    3411 
   Size:        509    1311    1591

  Fragments arranged by size: 

                1591    1311     509
 
BglI GCCn_nnn'nGGC

Cuts at:      0     448    3411 
   Size:        448    2963
 
BglII A'GATC_T

Cuts at:      0    1364    3411 
   Size:       1364    2047
 
BlpI GC'TnA_GC

Cuts at:      0    1417    2095    2770    3411 
   Size:       1417     678     675     641

  Fragments arranged by size: 

                1417     678     675     641
 
Bme1580I G_kGCm'C

Cuts at:      0    1655    2284    2638    3104    3411 
   Size:       1655     629     354     466     307

  Fragments arranged by size: 

                1655     629     466     354     307
 
BmgBI CAC'GTC

Cuts at:      0     891    1994    3411 
   Size:        891    1103    1417

  Fragments arranged by size: 

                1417    1103     891
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2349    3411 
   Size:       2349    1062
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      72      94     586    2167    2566    2950    3411 
   Size:         72      22     492    1581     399     384     461

  Fragments arranged by size: 

                1581     492     461     399     384      72      22
 
Bpu10I CC'TnA_GC

Cuts at:      0     913    1489    3345    3411 
   Size:        913     576    1856      66

  Fragments arranged by size: 

                1856     913     576      66
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     713    2337    3411 
   Size:        713    1624    1074

  Fragments arranged by size: 

                1624    1074     713
 
BsaAI yAC'GTr

Cuts at:      0    1413    2259    3411 
   Size:       1413     846    1152

  Fragments arranged by size: 

                1413    1152     846
 
BsaHI Gr'CG_yC

Cuts at:      0     551    1217    1630    2558    3332    3411 
   Size:        551     666     413     928     774      79

  Fragments arranged by size: 

                 928     774     666     551     413      79
 
BsaWI w'CCGG_w

Cuts at:      0    2087    3248    3411 
   Size:       2087    1161     163

  Fragments arranged by size: 

                2087    1161     163
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      70     100    1678    1708    2164    2194    3411 
   Size:         70      30    1578      30     456      30    1217

  Fragments arranged by size: 

                1578    1217     456      70      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     358     460    1102    1477    2173    2260    3139    3142 
   Size:        358     102     642     375     696      87     879       3

Cuts at:   3142    3322    3411 
   Size:        180      89

  Fragments arranged by size: 

                 879     696     642     375     358     180     102      89
                  87       3
 
BseYI C'CCAG_C

Cuts at:      0      82     546    2453    2762    2852    3411 
   Size:         82     464    1907     309      90     559

  Fragments arranged by size: 

                1907     559     464     309      90      82
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     640    1393    2634    2867    3145    3411 
   Size:        160     480     753    1241     233     278     266

  Fragments arranged by size: 

                1241     753     480     278     266     233     160
 
BsiHKAI G_wGCw'C

Cuts at:      0    1418    1655    2638    3411 
   Size:       1418     237     983     773

  Fragments arranged by size: 

                1418     983     773     237
 
BsmI GAATG_Cn'

Cuts at:      0     696    1574    3318    3411 
   Size:        696     878    1744      93

  Fragments arranged by size: 

                1744     878     696      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2713    3411 
   Size:       2713     698
 
Bsp1286I G_dGCh'C

Cuts at:      0    1418    1597    1655    2284    2323    2638    2950    3104 
   Size:       1418     179      58     629      39     315     312     154

Cuts at:   3104    3411 
   Size:        307

  Fragments arranged by size: 

                1418     629     315     312     307     179     154      58
                  39
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     599    1415    3411 
   Size:        599     816    1996

  Fragments arranged by size: 

                1996     816     599
 
BsrBI CCG'CTC

Cuts at:      0     896    3411 
   Size:        896    2515
 
BsrDI GCAATG_nn'

Cuts at:      0    2540    3411 
   Size:       2540     871
 
BsrFI r'CCGG_y

Cuts at:      0     594    1236    2044    3063    3272    3411 
   Size:        594     642     808    1019     209     139

  Fragments arranged by size: 

                1019     808     642     594     209     139
 
BsrGI T'GTAC_A

Cuts at:      0     638    1718    3411 
   Size:        638    1080    1693

  Fragments arranged by size: 

                1693    1080     638
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2019    3411 
   Size:       2019    1392
 
BstEII G'GTnAC_C

Cuts at:      0    2517    3411 
   Size:       2517     894
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     951    3411 
   Size:        951    2460
 
BstYI r'GATC_y

Cuts at:      0     770    1034    1145    1364    1767    2054    3411 
   Size:        770     264     111     219     403     287    1357

  Fragments arranged by size: 

                1357     770     403     287     264     219     111
 
Bsu36I CC'TnA_GG

Cuts at:      0    2357    3411 
   Size:       2357    1054
 
BtgI C'CryG_G

Cuts at:      0     122     834    1220    1427    1569    1965    2204    2486 
   Size:        122     712     386     207     142     396     239     282

Cuts at:   2486    2522    2552    3411 
   Size:         36      30     859

  Fragments arranged by size: 

                 859     712     396     386     282     239     207     142
                 122      36      30
 
BtsI GCAGTG_nn'

Cuts at:      0     756    1513    2617    3411 
   Size:        756     757    1104     794

  Fragments arranged by size: 

                1104     794     757     756
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     104     139    3411 
   Size:        104      35    3272

  Fragments arranged by size: 

                3272     104      35
 
DraI TTT'AAA

Cuts at:      0     662    1029    3411 
   Size:        662     367    2382

  Fragments arranged by size: 

                2382     662     367
 
DraIII CAC_nnn'GTG

Cuts at:      0    2262    3411 
   Size:       2262    1149
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1270    2671    3411 
   Size:       1270    1401     740

  Fragments arranged by size: 

                1401    1270     740
 
EaeI y'GGCC_r

Cuts at:      0    1258    1529    2042    3065    3411 
   Size:       1258     271     513    1023     346

  Fragments arranged by size: 

                1258    1023     513     346     271
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1063    1393    3259    3411 
   Size:       1063     330    1866     152

  Fragments arranged by size: 

                1866    1063     330     152
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      72      94     355     567     586     701     750    1171 
   Size:         72      22     261     212      19     115      49     421

Cuts at:   1171    1527    2167    2566    2950    3411 
   Size:        356     640     399     384     461

  Fragments arranged by size: 

                 640     461     421     399     384     356     261     212
                 115      72      49      22      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1042    3411 
   Size:       1042    2369
 
EcoO109I rG'GnC_Cy

Cuts at:      0     258    2136    2711    3015    3101    3411 
   Size:        258    1878     575     304      86     310

  Fragments arranged by size: 

                1878     575     310     304     258      86
 
EcoRI G'AATT_C

Cuts at:      0     466    2575    3411 
   Size:        466    2109     836

  Fragments arranged by size: 

                2109     836     466
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     552    3411 
   Size:        552    2859
 
HaeII r_GCGC'y

Cuts at:      0     554    1220    1633    2227    3335    3411 
   Size:        554     666     413     594    1108      76

  Fragments arranged by size: 

                1108     666     594     554     413      76
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     310     342    1961    1993    3411 
   Size:        310      32    1619      32    1418

  Fragments arranged by size: 

                1619    1418     310      32      32
 
HincII GTy'rAC

Cuts at:      0     731    3411 
   Size:        731    2680
 
HindIII A'AGCT_T

Cuts at:      0    1642    3411 
   Size:       1642    1769
 
KasI G'GCGC_C

Cuts at:      0     550    1216    1629    3331    3411 
   Size:        550     666     413    1702      80

  Fragments arranged by size: 

                1702     666     550     413      80
 
KpnI G_GTAC'C

Cuts at:      0    2406    3411 
   Size:       2406    1005
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2360    3411 
   Size:       2360    1051
 
MscI TGG'CCA

Cuts at:      0    1260    1531    3411 
   Size:       1260     271    1880

  Fragments arranged by size: 

                1880    1260     271
 
MslI CAynn'nnrTG

Cuts at:      0     634    1525    1996    2491    2509    2779    2843    3411 
   Size:        634     891     471     495      18     270      64     568

  Fragments arranged by size: 

                 891     634     568     495     471     270      64      18
            
 
MspA1I CmG'CkG

Cuts at:      0      98     624     836    2397    2453    2509    2554    2766 
   Size:         98     526     212    1561      56      56      45     212

Cuts at:   2766    3021    3411 
   Size:        255     390

  Fragments arranged by size: 

                1561     526     390     255     212     212      98      56
                  56      45
 
NaeI GCC'GGC

Cuts at:      0    3065    3274    3411 
   Size:       3065     209     137

  Fragments arranged by size: 

                3065     209     137
 
NarI GG'CG_CC

Cuts at:      0     551    1217    1630    3332    3411 
   Size:        551     666     413    1702      79

  Fragments arranged by size: 

                1702     666     551     413      79
 
NcoI C'CATG_G

Cuts at:      0    1427    1569    2204    2486    3411 
   Size:       1427     142     635     282     925

  Fragments arranged by size: 

                1427     925     635     282     142
 
NgoMIV G'CCGG_C

Cuts at:      0    3063    3272    3411 
   Size:       3063     209     139

  Fragments arranged by size: 

                3063     209     139
 
NspI r_CATG'y

Cuts at:      0     399     678    1340    2241    3411 
   Size:        399     279     662     901    1170

  Fragments arranged by size: 

                1170     901     662     399     279
 
PasI CC'CwG_GG

Cuts at:      0     960    3411 
   Size:        960    2451
 
PciI A'CATG_T

Cuts at:      0     674    2237    3411 
   Size:        674    1563    1174

  Fragments arranged by size: 

                1563    1174     674
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     429    2859    3411 
   Size:        429    2430     552

  Fragments arranged by size: 

                2430     552     429
 
PfoI T'CCnGG_A

Cuts at:      0     563    2168    3071    3411 
   Size:        563    1605     903     340

  Fragments arranged by size: 

                1605     903     563     340
 
PmlI CAC'GTG

Cuts at:      0    1413    2259    3411 
   Size:       1413     846    1152

  Fragments arranged by size: 

                1413    1152     846
 
PpuMI rG'GwC_Cy

Cuts at:      0     258    2136    2711    3015    3411 
   Size:        258    1878     575     304     396

  Fragments arranged by size: 

                1878     575     396     304     258
 
PshAI GACnn'nnGTC

Cuts at:      0    3217    3411 
   Size:       3217     194
 
PspOMI G'GGCC_C

Cuts at:      0    3100    3411 
   Size:       3100     311
 
PspXI vC'TCGA_Gb

Cuts at:      0    2829    3411 
   Size:       2829     582
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    3034    3066    3411 
   Size:       3034      32     345

  Fragments arranged by size: 

                3034     345      32
 
PstI C_TGCA'G

Cuts at:      0     305    2550    2909    3026    3184    3206    3411 
   Size:        305    2245     359     117     158      22     205

  Fragments arranged by size: 

                2245     359     305     205     158     117      22
 
PvuII CAG'CTG

Cuts at:      0     624    2509    2766    3411 
   Size:        624    1885     257     645

  Fragments arranged by size: 

                1885     645     624     257
 
RsrII CG'GwC_CG

Cuts at:      0    1050    3411 
   Size:       1050    2361
 
SacII CC_GC'GG

Cuts at:      0     837    2555    3411 
   Size:        837    1718     856

  Fragments arranged by size: 

                1718     856     837
 
SexAI A'CCwGG_T

Cuts at:      0    2893    3411 
   Size:       2893     518
 
SfcI C'TryA_G

Cuts at:      0     301    2546    2905    3022    3180    3202    3411 
   Size:        301    2245     359     117     158      22     209

  Fragments arranged by size: 

                2245     359     301     209     158     117      22
 
SfoI GGC'GCC

Cuts at:      0     552    1218    1631    3333    3411 
   Size:        552     666     413    1702      78

  Fragments arranged by size: 

                1702     666     552     413      78
 
SgrAI Cr'CCGG_yG

Cuts at:      0    3272    3411 
   Size:       3272     139
 
SmaI CCC'GGG

Cuts at:      0    1960    2287    3411 
   Size:       1960     327    1124

  Fragments arranged by size: 

                1960    1124     327
 
SmlI C'TyrA_G

Cuts at:      0    2829    3411 
   Size:       2829     582
 
SphI G_CATG'C

Cuts at:      0    1340    3411 
   Size:       1340    2071
 
SspI AAT'ATT

Cuts at:      0     742    3411 
   Size:        742    2669
 
StuI AGG'CCT

Cuts at:      0    1468    2009    2413    3411 
   Size:       1468     541     404     998

  Fragments arranged by size: 

                1468     998     541     404
 
StyI C'CwwG_G

Cuts at:      0     866    1427    1569    2204    2486    3411 
   Size:        866     561     142     635     282     925

  Fragments arranged by size: 

                 925     866     635     561     282     142
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     492    1067    1399    1573    2042    3411 
   Size:        492     575     332     174     469    1369

  Fragments arranged by size: 

                1369     575     492     469     332     174
 
TatI w'GTAC_w

Cuts at:      0     311     638    1718    3411 
   Size:        311     327    1080    1693

  Fragments arranged by size: 

                1693    1080     327     311
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2617    2649    3411 
   Size:       2617      32     762

  Fragments arranged by size: 

                2617     762      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1003    3411 
   Size:       1003    2408
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     216     731     785    3087    3411 
   Size:        216     515      54    2302     324

  Fragments arranged by size: 

                2302     515     324     216      54
 
XhoI C'TCGA_G

Cuts at:      0    2829    3411 
   Size:       2829     582
 
XmaI C'CCGG_G

Cuts at:      0    1958    2285    3411 
   Size:       1958     327    1126

  Fragments arranged by size: 

                1958    1126     327
 
XmnI GAAnn'nnTTC

Cuts at:      0     973    1156    3411 
   Size:        973     183    2255

  Fragments arranged by size: 

                2255     973     183
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AleI    AlwNI
     ApaI    ApaLI     ApoI     AseI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI     DraI
   DraIII     DrdI     EaeI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI    PvuII    RsrII    SacII    SexAI     SfcI
     SfoI    SgrAI     SmaI     SmlI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AgeI     AhdI     AlfI     AloI     AscI
    AsiSI    AvrII     BaeI    BfrBI     BmrI     BmtI    BpuEI    BsaBI
    BsiEI    BsiWI    BspEI    BspHI   BssHII    BssSI    BstBI  BstZ17I
    BtgZI     ClaI     EagI     EarI  EcoICRI    EcoRV     FseI     FspI
    FspAI     HpaI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PmeI     PpiI     PsiI     PvuI     SacI     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SgfI    SnaBI     SpeI
     SrfI     SwaI     XbaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com