Restriction map for pF1KE3300
 (Linear) (Six-base) MAPSORT of: KIEE3300.seq  Check: 6458  from: 1  to: 7665



KIEE3300.orf

 With 182 enzymes: SgfI * 

                        May 26, 2016 12:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     355     529     589     703    1054    1204    1405    1771 
   Size:        355     174      60     114     351     150     201     366

Cuts at:   1771    1858    2083    2110    2143    2308    2341    2917    2998 
   Size:         87     225      27      33     165      33     576      81

Cuts at:   2998    3025    3145    3484    3598    3736    3769    3970    4120 
   Size:         27     120     339     114     138      33     201     150

Cuts at:   4120    6707    6833    7140    7665 
   Size:       2587     126     307     525

  Fragments arranged by size: 

                2587     576     525     366     355     351     339     307
                 225     201     201     174     165     150     150     138
                 126     120     114     114      87      81      60      33
                  33      33      27      27
 
AatII G_ACGT'C

Cuts at:      0     716    1358    5012    5621    6062    7665 
   Size:        716     642    3654     609     441    1603

  Fragments arranged by size: 

                3654    1603     716     642     609     441
 
AccI GT'mk_AC

Cuts at:      0    2603    5027    7665 
   Size:       2603    2424    2638

  Fragments arranged by size: 

                2638    2603    2424
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1909    2331    2907    4482    4602    5384    5410    6163 
   Size:       1909     422     576    1575     120     782      26     753

Cuts at:   6163    7665 
   Size:       1502

  Fragments arranged by size: 

                1909    1575    1502     782     753     576     422     120
                  26
 
AfeI AGC'GCT

Cuts at:      0    3787    5114    7665 
   Size:       3787    1327    2551

  Fragments arranged by size: 

                3787    2551    1327
 
AflIII A'CryG_T

Cuts at:      0    2324    2413    2473    2900    4030    4409    7665 
   Size:       2324      89      60     427    1130     379    3256

  Fragments arranged by size: 

                3256    2324    1130     427     379      89      60
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3693    7557    7665 
   Size:       3693    3864     108

  Fragments arranged by size: 

                3864    3693     108
 
AleI CACnn'nnGTG

Cuts at:      0     725    1654    2992    3185    3730    4720    4807    5092 
   Size:        725     929    1338     193     545     990      87     285

Cuts at:   5092    7665 
   Size:       2573

  Fragments arranged by size: 

                2573    1338     990     929     725     545     285     193
                  87
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1117    1450    2467    5561    5908    7665 
   Size:       1117     333    1017    3094     347    1757

  Fragments arranged by size: 

                3094    1757    1117    1017     347     333
 
AlwNI CAG_nnn'CTG

Cuts at:      0     465    1320    1493    1946    2510    3050    3263    3422 
   Size:        465     855     173     453     564     540     213     159

Cuts at:   3422    3458    4169    4479    5541    5937    7101    7188    7335 
   Size:         36     711     310    1062     396    1164      87     147

Cuts at:   7335    7443    7665 
   Size:        108     222

  Fragments arranged by size: 

                1164    1062     855     711     564     540     465     453
                 396     310     222     213     173     159     147     108
                  87      36
 
ApaI G_GGCC'C

Cuts at:      0     163     287    1219    1339    4129    4141    6879    7342 
   Size:        163     124     932     120    2790      12    2738     463

Cuts at:   7342    7665 
   Size:        323

  Fragments arranged by size: 

                2790    2738     932     463     323     163     124     120
                  12
 
ApaLI G'TGCA_C

Cuts at:      0    1453    1659    2328    2904    3132    3178    3646    7357 
   Size:       1453     206     669     576     228      46     468    3711

Cuts at:   7357    7665 
   Size:        308

  Fragments arranged by size: 

                3711    1453     669     576     468     308     228     206
                  46
 
ApoI r'AATT_y

Cuts at:      0    4255    6882    7665 
   Size:       4255    2627     783

  Fragments arranged by size: 

                4255    2627     783
 
AvaI C'yCGr_G

Cuts at:      0     556    1203    1953    2113    4354    4696    6206    6757 
   Size:        556     647     750     160    2241     342    1510     551

Cuts at:   6757    7665 
   Size:        908

  Fragments arranged by size: 

                2241    1510     908     750     647     556     551     342
                 160
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    4032    4065    7665 
   Size:       4032      33    3600

  Fragments arranged by size: 

                4032    3600      33
 
BamHI G'GATC_C

Cuts at:      0    2930    7665 
   Size:       2930    4735
 
BanI G'GyrC_C

Cuts at:      0      15     343     577     691     808    1039    1255    1618 
   Size:         15     328     234     114     117     231     216     363

Cuts at:   1618    1846    1957    2071    2296    2467    2986    3100    3586 
   Size:        228     111     114     225     171     519     114     486

Cuts at:   3586    3724    3838    3902    3958    4024    5407    5749    6734 
   Size:        138     114      64      56      66    1383     342     985

Cuts at:   6734    6841    6954    6990    7509    7665 
   Size:        107     113      36     519     156

  Fragments arranged by size: 

                1383     985     519     519     486     363     342     328
                 234     231     228     225     216     171     156     138
                 117     114     114     114     114     113     111     107
                  66      64      56      36      15
 
BanII G_rGCy'C

Cuts at:      0     163     287    1153    1219    1303    1339    2629    2761 
   Size:        163     124     866      66      84      36    1290     132

Cuts at:   2761    2971    3362    3706    4129    4141    4214    4228    4268 
   Size:        210     391     344     423      12      73      14      40

Cuts at:   4268    4787    5357    5483    6381    6862    6879    7267    7342 
   Size:        519     570     126     898     481      17     388      75

Cuts at:   7342    7384    7665 
   Size:         42     281

  Fragments arranged by size: 

                1290     898     866     570     519     481     423     391
                 388     344     281     210     163     132     126     124
                  84      75      73      66      42      40      36      17
                  14      12
 
BbeI G_GCGC'C

Cuts at:      0      19    1043    3906    4028    5753    7665 
   Size:         19    1024    2863     122    1725    1912

  Fragments arranged by size: 

                2863    1912    1725    1024     122      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    4806    5919    7665 
   Size:       4806    1113    1746

  Fragments arranged by size: 

                4806    1746    1113
 
BbvCI CC'TCA_GC

Cuts at:      0     190    7665 
   Size:        190    7475
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2026    2060    3576    3610    3868    3902    7665 
   Size:       2026      34    1516      34     258      34    3763

  Fragments arranged by size: 

                3763    2026    1516     258      34      34      34
 
BfrBI ATG'CAT

Cuts at:      0    1127    4088    5976    6315    7665 
   Size:       1127    2961    1888     339    1350

  Fragments arranged by size: 

                2961    1888    1350    1127     339
 
BglI GCCn_nnn'nGGC

Cuts at:      0      98     392     575     707     740     923    1553    1730 
   Size:         98     294     183     132      33     183     630     177

Cuts at:   1730    1778    1844    2117    2342    2576    2639    2690    2804 
   Size:         48      66     273     225     234      63      51     114

Cuts at:   2804    2918    3095    3146    3260    3404    3632    3770    4013 
   Size:        114     177      51     114     144     228     138     243

Cuts at:   4013    4076    4178    6013    7286    7665 
   Size:         63     102    1835    1273     379

  Fragments arranged by size: 

                1835    1273     630     379     294     273     243     234
                 228     225     183     183     177     177     144     138
                 132     114     114     114     102      98      66      63
                  63      51      51      48      33
 
BlpI GC'TnA_GC

Cuts at:      0    4786    7266    7665 
   Size:       4786    2480     399

  Fragments arranged by size: 

                4786    2480     399
 
Bme1580I G_kGCm'C

Cuts at:      0     163     270     287     346     662     694    1219    1339 
   Size:        163     107      17      59     316      32     525     120

Cuts at:   1339    1457    1549    1663    1849    1960    2074    2332    2413 
   Size:        118      92     114     186     111     114     258      81

Cuts at:   2413    2502    2818    2908    3136    3182    3256    3290    3589 
   Size:         89     316      90     228      46      74      34     299

Cuts at:   3589    3637    3650    3961    4009    4129    4141    4249    4631 
   Size:         48      13     311      48     120      12     108     382

Cuts at:   4631    5412    6394    6689    6737    6879    7342    7361    7665 
   Size:        781     982     295      48     142     463      19     304

  Fragments arranged by size: 

                 982     781     525     463     382     316     316     311
                 304     299     295     258     228     186     163     142
                 120     120     118     114     114     111     108     107
                  92      90      89      81      74      59      48      48
                  48      46      34      32      19      17      13      12
            
 
BmgBI CAC'GTC

Cuts at:      0    1236    3285    5094    6636    7326    7397    7665 
   Size:       1236    2049    1809    1542     690      71     268

  Fragments arranged by size: 

                2049    1809    1542    1236     690     268      71
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1571    3231    6108    7345    7431    7665 
   Size:       1571    1660    2877    1237      86     234

  Fragments arranged by size: 

                2877    1660    1571    1237     234      86
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     615    1386    2574    3093    3579    7623    7665 
   Size:        615     771    1188     519     486    4044      42

  Fragments arranged by size: 

                4044    1188     771     615     519     486      42
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1806    5053    5473    5704    5863    6052    6628    6846 
   Size:       1806    3247     420     231     159     189     576     218

Cuts at:   6846    7593    7665 
   Size:        747      72

  Fragments arranged by size: 

                3247    1806     747     576     420     231     218     189
                 159      72
 
Bpu10I CC'TnA_GC

Cuts at:      0     190    7003    7514    7665 
   Size:        190    6813     511     151

  Fragments arranged by size: 

                6813     511     190     151
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    5365    6430    6511    7665 
   Size:       5365    1065      81    1154

  Fragments arranged by size: 

                5365    1154    1065      81
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      79    1795    2707    5164    5449    5779    6217    7665 
   Size:         79    1716     912    2457     285     330     438    1448

  Fragments arranged by size: 

                2457    1716    1448     912     438     330     285      79
            
 
BsaAI yAC'GTr

Cuts at:      0     225     657    2325    2414    2474    2903    4031    5214 
   Size:        225     432    1668      89      60     429    1128    1183

Cuts at:   5214    7665 
   Size:       2451

  Fragments arranged by size: 

                2451    1668    1183    1128     432     429     225      89
                  60
 
BsaBI GATnn'nnATC

Cuts at:      0    5938    7665 
   Size:       5938    1727
 
BsaHI Gr'CG_yC

Cuts at:      0      16     713    1040    1355    1391    1748    3830    3903 
   Size:         16     697     327     315      36     357    2082      73

Cuts at:   3903    4025    4925    5009    5603    5618    5750    5989    6059 
   Size:        122     900      84     594      15     132     239      70

Cuts at:   6059    7447    7604    7665 
   Size:       1388     157      61

  Fragments arranged by size: 

                2082    1388     900     697     594     357     327     315
                 239     157     132     122      84      73      70      61
                  36      16      15
 
BsaWI w'CCGG_w

Cuts at:      0    5468    5942    7650    7665 
   Size:       5468     474    1708      15

  Fragments arranged by size: 

                5468    1708     474      15
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     361     391    1135    1165    1408    1438    1717    1747 
   Size:        361      30     744      30     243      30     279      30

Cuts at:   1747    1804    1834    2007    2037    2743    2773    3604    3634 
   Size:         57      30     173      30     706      30     831      30

Cuts at:   3634    4648    4678    7453    7483    7665 
   Size:       1014      30    2775      30     182

  Fragments arranged by size: 

                2775    1014     831     744     706     361     279     243
                 182     173      57      30      30      30      30      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2842    3183    3559    4351    4882    4885    5055    5491 
   Size:       2842     341     376     792     531       3     170     436

Cuts at:   5491    5722    6196    7461    7665 
   Size:        231     474    1265     204

  Fragments arranged by size: 

                2842    1265     792     531     474     436     376     341
                 231     204     170       3
 
BseYI C'CCAG_C

Cuts at:      0      74     502    1251    1296    2503    3256    3289    3451 
   Size:         74     428     749      45    1207     753      33     162

Cuts at:   3451    4418    4910    4932    5124    6384    6481    6771    7105 
   Size:        967     492      22     192    1260      97     290     334

Cuts at:   7105    7124    7145    7342    7492    7665 
   Size:         19      21     197     150     173

  Fragments arranged by size: 

                1260    1207     967     753     749     492     428     334
                 290     197     192     173     162     150      97      74
                  45      33      22      21      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1176    1483    2550    2748    2862    2943    3462    3741 
   Size:       1176     307    1067     198     114      81     519     279

Cuts at:   3741    3874    4452    4783    5077    5182    5185    5743    6079 
   Size:        133     578     331     294     105       3     558     336

Cuts at:   6079    6355    6478    7033    7159    7174    7348    7665 
   Size:        276     123     555     126      15     174     317

  Fragments arranged by size: 

                1176    1067     578     558     555     519     336     331
                 317     307     294     279     276     198     174     133
                 126     123     114     105      81      15       3
 
BsiEI CG_ry'CG

Cuts at:      0    4712    4863    5225    5723    6786    7665 
   Size:       4712     151     362     498    1063     879

  Fragments arranged by size: 

                4712    1063     879     498     362     151
 
BsiHKAI G_wGCw'C

Cuts at:      0    1249    1457    1663    1822    2332    2908    2971    3136 
   Size:       1249     208     206     159     510     576      63     165

Cuts at:   3136    3182    3448    3622    3650    4787    6140    7009    7267 
   Size:         46     266     174      28    1137    1353     869     258

Cuts at:   7267    7361    7520    7665 
   Size:         94     159     145

  Fragments arranged by size: 

                1353    1249    1137     869     576     510     266     258
                 208     206     174     165     159     159     145      94
                  63      46      28
 
BsmI GAATG_Cn'

Cuts at:      0    4150    5101    7575    7646    7665 
   Size:       4150     951    2474      71      19

  Fragments arranged by size: 

                4150    2474     951      71      19
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1374    5680    6820    7590    7611    7665 
   Size:       1374    4306    1140     770      21      54

  Fragments arranged by size: 

                4306    1374    1140     770      54      21
 
Bsp1286I G_dGCh'C

Cuts at:      0     163     270     287     346     662     694    1153    1219 
   Size:        163     107      17      59     316      32     459      66

Cuts at:   1219    1249    1303    1339    1457    1549    1663    1822    1849 
   Size:         30      54      36     118      92     114     159      27

Cuts at:   1849    1960    2074    2332    2413    2502    2629    2761    2818 
   Size:        111     114     258      81      89     127     132      57

Cuts at:   2818    2908    2971    3136    3182    3256    3290    3362    3448 
   Size:         90      63     165      46      74      34      72      86

Cuts at:   3448    3589    3622    3637    3650    3706    3961    4009    4129 
   Size:        141      33      15      13      56     255      48     120

Cuts at:   4129    4141    4214    4228    4249    4268    4631    4787    5357 
   Size:         12      73      14      21      19     363     156     570

Cuts at:   5357    5412    5483    6140    6381    6394    6689    6737    6862 
   Size:         55      71     657     241      13     295      48     125

Cuts at:   6862    6879    7009    7267    7342    7361    7384    7520    7665 
   Size:         17     130     258      75      19      23     136     145

  Fragments arranged by size: 

                 657     570     459     363     316     295     258     258
                 255     241     165     163     159     156     145     141
                 136     132     130     127     125     120     118     114
                 114     111     107      92      90      89      86      81
                  75      74      73      72      71      66      63      59
                  57      56      55      54      48      48      46      36
                  34      33      32      30      27      23      21      19
                  19      17      17      15      14      13      13      12
            
 
BspEI T'CCGG_A

Cuts at:      0    5942    7650    7665 
   Size:       5942    1708      15

  Fragments arranged by size: 

                5942    1708      15
 
BspHI T'CATG_A

Cuts at:      0    5654    7665 
   Size:       5654    2011
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      97     355     362     529     589     703     940    1054 
   Size:         97     258       7     167      60     114     237     114

Cuts at:   1054    1204    1405    1771    1858    2083    2110    2143    2308 
   Size:        150     201     366      87     225      27      33     165

Cuts at:   2308    2341    2917    2998    3025    3145    3484    3598    3736 
   Size:         33     576      81      27     120     339     114     138

Cuts at:   3736    3769    3970    4060    4120    5564    6707    6833    7140 
   Size:         33     201      90      60    1444    1143     126     307

Cuts at:   7140    7196    7665 
   Size:         56     469

  Fragments arranged by size: 

                1444    1143     576     469     366     339     307     258
                 237     225     201     201     167     165     150     138
                 126     120     114     114     114      97      90      87
                  81      60      60      56      33      33      33      27
                  27       7
 
BsrBI CCG'CTC

Cuts at:      0       9     648    5652    6414    6947    7665 
   Size:          9     639    5004     762     533     718

  Fragments arranged by size: 

                5004     762     718     639     533       9
 
BsrFI r'CCGG_y

Cuts at:      0     524     748    2633    2689    2732    3268    3517    4534 
   Size:        524     224    1885      56      43     536     249    1017

Cuts at:   4534    4711    5045    5190    5279    5519    5718    6785    7665 
   Size:        177     334     145      89     240     199    1067     880

  Fragments arranged by size: 

                1885    1067    1017     880     536     524     334     249
                 240     224     199     177     145      89      56      43
            
 
BssSI C'ACGA_G

Cuts at:      0      56     721    7665 
   Size:         56     665    6944

  Fragments arranged by size: 

                6944     665      56
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     200     253    1493    1607    1946    2057    2171    2885 
   Size:        200      53    1240     114     339     111     114     714

Cuts at:   2885    3050    3944    4169    4187    4479    5055    6563    7188 
   Size:        165     894     225      18     292     576    1508     625

Cuts at:   7188    7221    7665 
   Size:         33     444

  Fragments arranged by size: 

                1508    1240     894     714     625     576     444     339
                 292     225     200     165     114     114     111      53
                  33      18
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1401    1629    5094    5961    6703    7350    7665 
   Size:       1401     228    3465     867     742     647     315

  Fragments arranged by size: 

                3465    1401     867     742     647     315     228
 
BstYI r'GATC_y

Cuts at:      0    2930    5933    7665 
   Size:       2930    3003    1732

  Fragments arranged by size: 

                3003    2930    1732
 
Bsu36I CC'TnA_GG

Cuts at:      0    5309    7665 
   Size:       5309    2356
 
BtgI C'CryG_G

Cuts at:      0     510     570     704     945    1035    1086    1550    1839 
   Size:        510      60     134     241      90      51     464     289

Cuts at:   1839    2178    2573    2661    3525    3628    3834    3968    3987 
   Size:        339     395      88     864     103     206     134      19

Cuts at:   3987    5003    5013    5174    5372    5483    5570    5628    6017 
   Size:       1016      10     161     198     111      87      58     389

Cuts at:   6017    6596    6618    7665 
   Size:        579      22    1047

  Fragments arranged by size: 

                1047    1016     864     579     510     464     395     389
                 339     289     241     206     198     161     134     134
                 111     103      90      88      87      60      58      51
                  22      19      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      81     180    6282    7665 
   Size:         81      99    6102    1383

  Fragments arranged by size: 

                6102    1383      99      81
 
BtsI GCAGTG_nn'

Cuts at:      0     492    1455    1683    1794    2679    3387    3873    4402 
   Size:        492     963     228     111     885     708     486     529

Cuts at:   4402    4489    4629    5056    5077    5530    6649    7665 
   Size:         87     140     427      21     453    1119    1016

  Fragments arranged by size: 

                1119    1016     963     885     708     529     492     486
                 453     427     228     140     111      87      21
 
ClaI AT'CG_AT

Cuts at:      0    1700    1976    2036    7665 
   Size:       1700     276      60    5629

  Fragments arranged by size: 

                5629    1700     276      60
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     525     560    6907    6942    7665 
   Size:        525      35    6347      35     723

  Fragments arranged by size: 

                6347     723     525      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     228    3531    5923    7137    7665 
   Size:        228    3303    2392    1214     528

  Fragments arranged by size: 

                3303    2392    1214     528     228
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    5623    7665 
   Size:       5623    2042
 
EaeI y'GGCC_r

Cuts at:      0     116     456     546     750    1089    1506    3515    4157 
   Size:        116     340      90     204     339     417    2009     642

Cuts at:   4157    4506    4709    4833    4860    5096    5222    5292    5720 
   Size:        349     203     124      27     236     126      70     428

Cuts at:   5720    5801    6014    6200    6749    6783    7118    7278    7665 
   Size:         81     213     186     549      34     335     160     387

  Fragments arranged by size: 

                2009     642     549     428     417     387     349     340
                 339     335     236     213     204     203     186     160
                 126     124     116      90      81      70      34      27
            
 
EagI C'GGCC_G

Cuts at:      0    4709    4860    5222    5720    6783    7665 
   Size:       4709     151     362     498    1063     882

  Fragments arranged by size: 

                4709    1063     882     498     362     151
 
EarI CTCTTCn'nnn_

Cuts at:      0    4549    5699    7665 
   Size:       4549    1150    1966

  Fragments arranged by size: 

                4549    1966    1150
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    5001    6078    7665 
   Size:       5001    1077    1587

  Fragments arranged by size: 

                5001    1587    1077
 
EcoICRI GAG'CTC

Cuts at:      0    2969    4785    7265    7665 
   Size:       2969    1816    2480     400

  Fragments arranged by size: 

                2969    2480    1816     400
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1806    1909    2331    2907    4482    4602    5053    5384 
   Size:       1806     103     422     576    1575     120     451     331

Cuts at:   5384    5410    5473    5704    5863    6052    6163    6628    6846 
   Size:         26      63     231     159     189     111     465     218

Cuts at:   6846    7593    7665 
   Size:        747      72

  Fragments arranged by size: 

                1806    1575     747     576     465     451     422     331
                 231     218     189     159     120     111     103      72
                  63      26
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2143    5362    5842    7665 
   Size:       2143    3219     480    1823

  Fragments arranged by size: 

                3219    2143    1823     480
 
EcoO109I rG'GnC_Cy

Cuts at:      0     149     174     284     929    1336    1903    2183    3429 
   Size:        149      25     110     645     407     567     280    1246

Cuts at:   3429    4126    4138    4202    5448    6036    6522    6875    6876 
   Size:        697      12      64    1246     588     486     353       1

Cuts at:   6876    6997    7014    7098    7339    7665 
   Size:        121      17      84     241     326

  Fragments arranged by size: 

                1246    1246     697     645     588     567     486     407
                 353     326     280     241     149     121     110      84
                  64      25      17      12       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    4632    7665 
   Size:       4632    3033
 
FspI TGC'GCA

Cuts at:      0    3314    4756    4879    5560    6358    7665 
   Size:       3314    1442     123     681     798    1307

  Fragments arranged by size: 

                3314    1442    1307     798     681     123
 
HaeII r_GCGC'y

Cuts at:      0      19      37    1025    1043    3789    3906    4028    5116 
   Size:         19      18     988      18    2746     117     122    1088

Cuts at:   5116    5280    5753    5838    6772    7073    7665 
   Size:        164     473      85     934     301     592

  Fragments arranged by size: 

                2746    1088     988     934     592     473     301     164
                 122     117      85      19      18      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1231    1263    1334    1366    2257    2289    2371    2403 
   Size:       1231      32      71      32     891      32      82      32

Cuts at:   2403    3430    3462    3679    3711    3799    3831    4201    4233 
   Size:       1027      32     217      32      88      32     370      32

Cuts at:   4233    6625    6657    6916    6948    7543    7575    7665 
   Size:       2392      32     259      32     595      32      90

  Fragments arranged by size: 

                2392    1231    1027     891     595     370     259     217
                  90      88      82      71      32      32      32      32
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     537     972    1359    1467    2289    2604    3330    3687 
   Size:        537     435     387     108     822     315     726     357

Cuts at:   3687    3804    5601    6063    7665 
   Size:        117    1797     462    1602

  Fragments arranged by size: 

                1797    1602     822     726     537     462     435     387
                 357     315     117     108
 
KasI G'GCGC_C

Cuts at:      0      15    1039    3902    4024    5749    7665 
   Size:         15    1024    2863     122    1725    1916

  Fragments arranged by size: 

                2863    1916    1725    1024     122      15
 
MluI A'CGCG_T

Cuts at:      0    4409    7665 
   Size:       4409    3256
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     461    1178    2300    2414    4001    5756    7604    7613 
   Size:        461     717    1122     114    1587    1755    1848       9

Cuts at:   7613    7665 
   Size:         52

  Fragments arranged by size: 

                1848    1755    1587    1122     717     461     114      52
                   9
 
MscI TGG'CCA

Cuts at:      0     458     548    5294    7120    7665 
   Size:        458      90    4746    1826     545

  Fragments arranged by size: 

                4746    1826     545     458      90
 
MslI CAynn'nnrTG

Cuts at:      0     725    1055    1432    1654    2992    3185    3730    4720 
   Size:        725     330     377     222    1338     193     545     990

Cuts at:   4720    4807    5092    5212    5543    7624    7665 
   Size:         87     285     120     331    2081      41

  Fragments arranged by size: 

                2081    1338     990     725     545     377     331     330
                 285     222     193     120      87      41
 
MspA1I CmG'CkG

Cuts at:      0     263     383     608     845     900    1511    2108    2351 
   Size:        263     120     225     237      55     611     597     243

Cuts at:   2351    2675    2927    3245    3260    3395    3755    3908    3989 
   Size:        324     252     318      15     135     360     153      81

Cuts at:   3989    4329    4752    5015    5199    5551    5630    5672    5892 
   Size:        340     423     263     184     352      79      42     220

Cuts at:   5892    6369    6384    6869    7047    7277    7665 
   Size:        477      15     485     178     230     388

  Fragments arranged by size: 

                 611     597     485     477     423     388     360     352
                 340     324     318     263     263     252     243     237
                 230     225     220     184     178     153     135     120
                  81      79      55      42      15      15
 
NaeI GCC'GGC

Cuts at:      0     526    2635    2691    2734    3519    4536    4713    5192 
   Size:        526    2109      56      43     785    1017     177     479

Cuts at:   5192    5281    5720    6787    7665 
   Size:         89     439    1067     878

  Fragments arranged by size: 

                2109    1067    1017     878     785     526     479     439
                 177      89      56      43
 
NarI GG'CG_CC

Cuts at:      0      16    1040    3903    4025    5750    7665 
   Size:         16    1024    2863     122    1725    1915

  Fragments arranged by size: 

                2863    1915    1725    1024     122      16
 
NcoI C'CATG_G

Cuts at:      0     510    1086    5003    5570    6618    7665 
   Size:        510     576    3917     567    1048    1047

  Fragments arranged by size: 

                3917    1048    1047     576     567     510
 
NdeI CA'TA_TG

Cuts at:      0    6275    7665 
   Size:       6275    1390
 
NgoMIV G'CCGG_C

Cuts at:      0     524    2633    2689    2732    3517    4534    4711    5190 
   Size:        524    2109      56      43     785    1017     177     479

Cuts at:   5190    5279    5718    6785    7665 
   Size:         89     439    1067     880

  Fragments arranged by size: 

                2109    1067    1017     880     785     524     479     439
                 177      89      56      43
 
NotI GC'GGCC_GC

Cuts at:      0    5222    7665 
   Size:       5222    2443
 
NsiI A_TGCA'T

Cuts at:      0    1129    4090    5978    6317    7665 
   Size:       1129    2961    1888     339    1348

  Fragments arranged by size: 

                2961    1888    1348    1129     339
 
NspI r_CATG'y

Cuts at:      0     221    1127    1276    1437    2022    4088    4693    5565 
   Size:        221     906     149     161     585    2066     605     872

Cuts at:   5565    5899    5976    6030    6315    6588    7629    7665 
   Size:        334      77      54     285     273    1041      36

  Fragments arranged by size: 

                2066    1041     906     872     605     585     334     285
                 273     221     161     149      77      54      36
 
PasI CC'CwG_GG

Cuts at:      0     270    2227    3144    5589    7665 
   Size:        270    1957     917    2445    2076

  Fragments arranged by size: 

                2445    2076    1957     917     270
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     228    1236    2579    3263    4281    7665 
   Size:        228    1008    1343     684    1018    3384

  Fragments arranged by size: 

                3384    1343    1018    1008     684     228
 
PfoI T'CCnGG_A

Cuts at:      0     783    3697    4280    5867    7665 
   Size:        783    2914     583    1587    1798

  Fragments arranged by size: 

                2914    1798    1587     783     583
 
PmlI CAC'GTG

Cuts at:      0     225    2414    2474    2903    4031    7665 
   Size:        225    2189      60     429    1128    3634

  Fragments arranged by size: 

                3634    2189    1128     429     225      60
 
PpuMI rG'GwC_Cy

Cuts at:      0     174     929    1903    3429    4202    5448    6036    6522 
   Size:        174     755     974    1526     773    1246     588     486

Cuts at:   6522    7665 
   Size:       1143

  Fragments arranged by size: 

                1526    1246    1143     974     773     755     588     486
                 174
 
PshAI GACnn'nnGTC

Cuts at:      0     779    7665 
   Size:        779    6886
 
PspOMI G'GGCC_C

Cuts at:      0     159     283    1215    1335    4125    4137    6875    7338 
   Size:        159     124     932     120    2790      12    2738     463

Cuts at:   7338    7665 
   Size:        327

  Fragments arranged by size: 

                2790    2738     932     463     327     159     124     120
                  12
 
PstI C_TGCA'G

Cuts at:      0     262     667     997    1228    1325    2515    4390    4397 
   Size:        262     405     330     231      97    1190    1875       7

Cuts at:   4397    4484    5671    6944    7028    7193    7226    7665 
   Size:         87    1187    1273      84     165      33     439

  Fragments arranged by size: 

                1875    1273    1190    1187     439     405     330     262
                 231     165      97      87      84      33       7
 
PvuII CAG'CTG

Cuts at:      0     263     900    3755    4752    5199    6369    7665 
   Size:        263     637    2855     997     447    1170    1296

  Fragments arranged by size: 

                2855    1296    1170     997     637     447     263
 
SacI G_AGCT'C

Cuts at:      0    2971    4787    7267    7665 
   Size:       2971    1816    2480     398

  Fragments arranged by size: 

                2971    2480    1816     398
 
SacII CC_GC'GG

Cuts at:      0    3990    5016    5631    7665 
   Size:       3990    1026     615    2034

  Fragments arranged by size: 

                3990    2034    1026     615
 
SalI G'TCGA_C

Cuts at:      0    2602    7665 
   Size:       2602    5063
 
ScaI AGT'ACT

Cuts at:      0     871    4495    4603    7665 
   Size:        871    3624     108    3062

  Fragments arranged by size: 

                3624    3062     871     108
 
SexAI A'CCwGG_T

Cuts at:      0    3431    3737    6350    7133    7665 
   Size:       3431     306    2613     783     532

  Fragments arranged by size: 

                3431    2613     783     532     306
 
SfcI C'TryA_G

Cuts at:      0     258     663     993    1224    1321    2511    2694    3126 
   Size:        258     405     330     231      97    1190     183     432

Cuts at:   3126    3750    4287    4386    4393    4480    5667    6940    7024 
   Size:        624     537      99       7      87    1187    1273      84

Cuts at:   7024    7189    7222    7504    7665 
   Size:        165      33     282     161

  Fragments arranged by size: 

                1273    1190    1187     624     537     432     405     330
                 282     258     231     183     165     161      99      97
                  87      84      33       7
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    7286    7665 
   Size:       7286     379
 
SfoI GGC'GCC

Cuts at:      0      17    1041    3904    4026    5751    7665 
   Size:         17    1024    2863     122    1725    1914

  Fragments arranged by size: 

                2863    1914    1725    1024     122      17
 
SgrAI Cr'CCGG_yG

Cuts at:      0    5190    5279    7665 
   Size:       5190      89    2386

  Fragments arranged by size: 

                5190    2386      89
 
SmaI CCC'GGG

Cuts at:      0     558    6208    7665 
   Size:        558    5650    1457

  Fragments arranged by size: 

                5650    1457     558
 
SmlI C'TyrA_G

Cuts at:      0    5380    6445    6526    7665 
   Size:       5380    1065      81    1139

  Fragments arranged by size: 

                5380    1139    1065      81
 
SphI G_CATG'C

Cuts at:      0    1127    1276    1437    5899    5976    6030    6315    6588 
   Size:       1127     149     161    4462      77      54     285     273

Cuts at:   6588    7629    7665 
   Size:       1041      36

  Fragments arranged by size: 

                4462    1127    1041     285     273     161     149      77
                  54      36
 
SrfI GCCC'GGGC

Cuts at:      0     558    7665 
   Size:        558    7107
 
StuI AGG'CCT

Cuts at:      0     133     478    4948    5062    6494    7657    7665 
   Size:        133     345    4470     114    1432    1163       8

  Fragments arranged by size: 

                4470    1432    1163     345     133     114       8
 
StyI C'CwwG_G

Cuts at:      0     510    1086    5003    5570    6230    6518    6618    7254 
   Size:        510     576    3917     567     660     288     100     636

Cuts at:   7254    7665 
   Size:        411

  Fragments arranged by size: 

                3917     660     636     576     567     510     411     288
                 100
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     516     640     733     958    1243    2219    2674    2731 
   Size:        516     124      93     225     285     976     455      57

Cuts at:   2731    3250    3465    3769    3856    6733    6763    7138    7665 
   Size:        519     215     304      87    2877      30     375     527

  Fragments arranged by size: 

                2877     976     527     519     516     455     375     304
                 285     225     215     124      93      87      57      30
            
 
TatI w'GTAC_w

Cuts at:      0     371     869    4493    4601    6344    7665 
   Size:        371     498    3624     108    1743    1321

  Fragments arranged by size: 

                3624    1743    1321     498     371     108
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     361     393     491     523    1408    1440    1717    1749 
   Size:        361      32      98      32     885      32     277      32

Cuts at:   1749    2232    2264    2629    2661    2922    2954    4564    4596 
   Size:        483      32     365      32     261      32    1610      32

Cuts at:   4596    4648    4680    7453    7485    7665 
   Size:         52      32    2773      32     180

  Fragments arranged by size: 

                2773    1610     885     483     365     361     277     261
                 180      98      52      32      32      32      32      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1747    7665 
   Size:       1747    5918
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2478    3151    5028    5838    6237    6432    6759    6852 
   Size:       2478     673    1877     810     399     195     327      93

Cuts at:   6852    7113    7281    7665 
   Size:        261     168     384

  Fragments arranged by size: 

                2478    1877     810     673     399     384     327     261
                 195     168      93
 
XmaI C'CCGG_G

Cuts at:      0     556    6206    7665 
   Size:        556    5650    1459

  Fragments arranged by size: 

                5650    1459     556
 
XmnI GAAnn'nnTTC

Cuts at:      0    4495    7665 
   Size:       4495    3170
 
ZraI GAC'GTC

Cuts at:      0     714    1356    5010    5619    6060    7665 
   Size:        714     642    3654     609     441    1605

  Fragments arranged by size: 

                3654    1605     714     642     609     441
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AhdI     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BssSI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
     FspI    HaeII    Hin4I   HincII     KasI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI
     NsiI     NspI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI
   PspOMI     PstI    PvuII     SacI    SacII     SalI     ScaI    SexAI
     SfcI     SfiI     SfoI    SgrAI     SmaI     SmlI     SphI     SrfI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AloI     AscI     AseI    AsiSI
    AvrII    BciVI     BclI    BglII     BmtI    BsiWI    BsrDI    BsrGI
   BssHII    BstBI   BstEII  BstZ17I     DraI    EcoRI    EcoRV     FseI
    FspAI  HindIII     HpaI     KpnI     MfeI     NheI     NruI     PacI
     PciI     PmeI     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII
    SanDI     SapI     SbfI     SgfI    SnaBI     SpeI     SspI     SwaI
     XbaI     XhoI
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