Restriction map for pF1KE2657
 (Circular) (Six-base) MAPSORT of: pF1KE2657.seq  Check: 8681  from: 1  to: 3704



pF1KE2657  3704 bp

 With 182 enzymes: SgfI * 

                        December 21, 2016 11:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    472     472 
   Size:       3704
 
AccI GT'mk_AC

Cuts at:    742     742 
   Size:       3704
 
Acc65I G'GTAC_C

Cuts at:    720     720 
   Size:       3704
 
AclI AA'CG_TT

Cuts at:   3544    3544 
   Size:       3704
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1529    1961    2708    2976    1529 
   Size:        432     747     268    2257

  Fragments arranged by size: 

                2257     747     432     268
 
AflIII A'CryG_T

Cuts at:   1056    2160    3137    1056 
   Size:       1104     977    1623

  Fragments arranged by size: 

                1623    1104     977
 
AgeI A'CCGG_T

Cuts at:   2092    2092 
   Size:       3704
 
AlwNI CAG_nnn'CTG

Cuts at:    432     435     457    1009    2576     432 
   Size:          3      22     552    1567    1560

  Fragments arranged by size: 

                1567    1560     552      22       3
 
ApaLI G'TGCA_C

Cuts at:    199    2474     199 
   Size:       2275    1429
 
ApoI r'AATT_y

Cuts at:    708    3057    3313     708 
   Size:       2349     256    1099

  Fragments arranged by size: 

                2349    1099     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3704
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3704
 
AvaI C'yCGr_G

Cuts at:    167     724     167 
   Size:        557    3147
 
BamHI G'GATC_C

Cuts at:    729     729 
   Size:       3704
 
BanI G'GyrC_C

Cuts at:    330     460     681     720    1134    1382    1417     330 
   Size:        130     221      39     414     248      35    2617

  Fragments arranged by size: 

                2617     414     248     221     130      39      35
 
BanII G_rGCy'C

Cuts at:    145     267     718    1748     145 
   Size:        122     451    1030    2101

  Fragments arranged by size: 

                2101    1030     451     122
 
BbeI G_GCGC'C

Cuts at:    334    1138    1386     334 
   Size:        804     248    2652

  Fragments arranged by size: 

                2652     804     248
 
BciVI GTATCCnnnnn_n'

Cuts at:   1602    2363    3704    1602 
   Size:        761    1341    1602

  Fragments arranged by size: 

                1602    1341     761
 
BclI T'GATC_A

Cuts at:   1224    1224 
   Size:       3704
 
BglI GCCn_nnn'nGGC

Cuts at:    179     179 
   Size:       3704
 
BglII A'GATC_T

Cuts at:   1219    3256    1219 
   Size:       2037    1667
 
BlpI GC'TnA_GC

Cuts at:    618     816     618 
   Size:        198    3506
 
Bme1580I G_kGCm'C

Cuts at:    203    1329    1422    2478     203 
   Size:       1126      93    1056    1429

  Fragments arranged by size: 

                1429    1126    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    190     497     916     997    1332    3476     190 
   Size:        307     419      81     335    2144     418

  Fragments arranged by size: 

                2144     419     418     335     307      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    286    2071     286 
   Size:       1785    1919
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    334     473     334 
   Size:        139    3565
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    529     880    1759    2251    2549     529 
   Size:        351     879     492     298    1684

  Fragments arranged by size: 

                1684     879     492     351     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    283    3408     283 
   Size:       3125     579
 
BsaAI yAC'GTr

Cuts at:   1057    1057 
   Size:       3704
 
BsaBI GATnn'nnATC

Cuts at:      6    1218    3255       6 
   Size:       1212    2037     455

  Fragments arranged by size: 

                2037    1212     455
 
BsaHI Gr'CG_yC

Cuts at:    331    1135    1383    2085    3581     331 
   Size:        804     248     702    1496     454

  Fragments arranged by size: 

                1496     804     702     454     248
 
BsaWI w'CCGG_w

Cuts at:    628     893    1414    2092    2366    2513    2924     628 
   Size:        265     521     678     274     147     411    1408

  Fragments arranged by size: 

                1408     678     521     411     274     265     147
 
BseYI C'CCAG_C

Cuts at:    341     588    1129    1887    2464    2960     341 
   Size:        247     541     758     577     496    1085

  Fragments arranged by size: 

                1085     758     577     541     496     247
 
BsiEI CG_ry'CG

Cuts at:    104    1246    1292    2500     104 
   Size:       1142      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1142      46
 
BsiHKAI G_wGCw'C

Cuts at:    203     368     718    1496    1686    2478     203 
   Size:        165     350     778     190     792    1429

  Fragments arranged by size: 

                1429     792     778     350     190     165
 
BsmI GAATG_Cn'

Cuts at:    182     553     182 
   Size:        371    3333
 
Bsp1286I G_dGCh'C

Cuts at:    145     203     267     368     718    1329    1422    1496    1686 
   Size:         58      64     101     350     611      93      74     190

Cuts at:   1686    1748    2478     145 
   Size:         62     730    1371

  Fragments arranged by size: 

                1371     730     611     350     190     101      93      74
                  64      62      58
 
BspEI T'CCGG_A

Cuts at:    628    2924     628 
   Size:       2296    1408
 
BspHI T'CATG_A

Cuts at:    476    2880     476 
   Size:       2404    1300
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    472     754     968    1270    1651     472 
   Size:        282     214     302     381    2525

  Fragments arranged by size: 

                2525     381     302     282     214
 
BsrBI CCG'CTC

Cuts at:     50     687    1996    2050    3534    3699      50 
   Size:        637    1309      54    1484     165      55

  Fragments arranged by size: 

                1484    1309     637     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1616    1616 
   Size:       3704
 
BsrFI r'CCGG_y

Cuts at:   1702    1883    2092    1702 
   Size:        181     209    3314

  Fragments arranged by size: 

                3314     209     181
 
BssSI C'ACGA_G

Cuts at:    409    1975    2333     409 
   Size:       1566     358    1780

  Fragments arranged by size: 

                1780    1566     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    188     467    1081     188 
   Size:        279     614    2811

  Fragments arranged by size: 

                2811     614     279
 
BstBI TT'CG_AA

Cuts at:   2065    2065 
   Size:       3704
 
BstEII G'GTnAC_C

Cuts at:    372     372 
   Size:       3704
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114     114 
   Size:       3704
 
BstYI r'GATC_y

Cuts at:      1     729    1196    1219    1554    1800    2812    2898    2910 
   Size:        728     467      23     335     246    1012      86      12

Cuts at:   2910    3256       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     728     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3704
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1654    1835    1998    1654 
   Size:        181     163    3360

  Fragments arranged by size: 

                3360     181     163
 
BtsI GCAGTG_nn'

Cuts at:    377     578     947     377 
   Size:        201     369    3134

  Fragments arranged by size: 

                3134     369     201
 
DraI TTT'AAA

Cuts at:    703     703 
   Size:       3704
 
DraIII CAC_nnn'GTG

Cuts at:    671     671 
   Size:       3704
 
DrdI GACnn_nn'nnGTC

Cuts at:   1410    2268    1410 
   Size:        858    2846
 
EaeI y'GGCC_r

Cuts at:    656    1289    1463    1854    1881    3226     656 
   Size:        633     174     391      27    1345    1134

  Fragments arranged by size: 

                1345    1134     633     391     174      27
 
EagI C'GGCC_G

Cuts at:   1289    1289 
   Size:       3704
 
EarI CTCTTCn'nnn_

Cuts at:   1727    1937    1727 
   Size:        210    3494
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1912    2222    2368    3514    1912 
   Size:        310     146    1146    2102

  Fragments arranged by size: 

                2102    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    716     716 
   Size:       3704
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    334     473    1529    1961    2708    2976     334 
   Size:        139    1056     432     747     268    1062

  Fragments arranged by size: 

                1062    1056     747     432     268     139
 
EcoO109I rG'GnC_Cy

Cuts at:    129     636     843     129 
   Size:        507     207    2990

  Fragments arranged by size: 

                2990     507     207
 
EcoRI G'AATT_C

Cuts at:    708     708 
   Size:       3704
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1723    1723 
   Size:       3704
 
FspI TGC'GCA

Cuts at:   1485    1485 
   Size:       3704
 
HaeII r_GCGC'y

Cuts at:    334    1138    1386    2408    3387     334 
   Size:        804     248    1022     979     651

  Fragments arranged by size: 

                1022     979     804     651     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    601     633    1546    1576    1578    1608     601 
   Size:         32     913      30       2      30    2697

  Fragments arranged by size: 

                2697     913      32      30      30       2
 
HincII GTy'rAC

Cuts at:    743     743 
   Size:       3704
 
KasI G'GCGC_C

Cuts at:    330    1134    1382     330 
   Size:        804     248    2652

  Fragments arranged by size: 

                2652     804     248
 
KpnI G_GTAC'C

Cuts at:    724     724 
   Size:       3704
 
MluI A'CGCG_T

Cuts at:   3137    3137 
   Size:       3704
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2375    2559    2375 
   Size:        184    3520
 
MscI TGG'CCA

Cuts at:   1465    1465 
   Size:       3704
 
MslI CAynn'nnrTG

Cuts at:    112     247     304     658    1693    1820     112 
   Size:        135      57     354    1035     127    1996

  Fragments arranged by size: 

                1996    1035     354     135     127      57
 
MspA1I CmG'CkG

Cuts at:    432     815    1129    1489    2502    2747     432 
   Size:        383     314     360    1013     245    1389

  Fragments arranged by size: 

                1389    1013     383     360     314     245
 
NaeI GCC'GGC

Cuts at:   1885    1885 
   Size:       3704
 
NarI GG'CG_CC

Cuts at:    331    1135    1383     331 
   Size:        804     248    2652

  Fragments arranged by size: 

                2652     804     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3704
 
NgoMIV G'CCGG_C

Cuts at:   1883    1883 
   Size:       3704
 
NspI r_CATG'y

Cuts at:    757    1257    2164     757 
   Size:        500     907    2297

  Fragments arranged by size: 

                2297     907     500
 
PciI A'CATG_T

Cuts at:   2160    2160 
   Size:       3704
 
PflMI CCAn_nnn'nTGG

Cuts at:    457     457 
   Size:       3704
 
PmeI GTTT'AAAC

Cuts at:    703     703 
   Size:       3704
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2843    2875    2843 
   Size:         32    3672
 
PpuMI rG'GwC_Cy

Cuts at:    129     636     129 
   Size:        507    3197
 
PstI C_TGCA'G

Cuts at:    560     751    1436     560 
   Size:        191     685    2828

  Fragments arranged by size: 

                2828     685     191
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3704
 
PvuII CAG'CTG

Cuts at:    432    1129    1489     432 
   Size:        697     360    2647

  Fragments arranged by size: 

                2647     697     360
 
RsrII CG'GwC_CG

Cuts at:   1899    1899 
   Size:       3704
 
SacI G_AGCT'C

Cuts at:    718     718 
   Size:       3704
 
SalI G'TCGA_C

Cuts at:    741     741 
   Size:       3704
 
SanDI GG'GwC_CC

Cuts at:    636     636 
   Size:       3704
 
SapI GCTCTTCn'nnn_

Cuts at:   1727    1937    1727 
   Size:        210    3494
 
SbfI CC_TGCA'GG

Cuts at:    751     751 
   Size:       3704
 
ScaI AGT'ACT

Cuts at:   3221    3221 
   Size:       3704
 
SexAI A'CCwGG_T

Cuts at:    368     368 
   Size:       3704
 
SfcI C'TryA_G

Cuts at:     33     556     747    1432    2425    2616      33 
   Size:        523     191     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     523     191     191
 
SfoI GGC'GCC

Cuts at:    332    1136    1384     332 
   Size:        804     248    2652

  Fragments arranged by size: 

                2652     804     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3704
 
SmaI CCC'GGG

Cuts at:    169     726     169 
   Size:        557    3147
 
SmlI C'TyrA_G

Cuts at:    508     859    1774    2266    2528     508 
   Size:        351     915     492     262    1684

  Fragments arranged by size: 

                1684     915     492     351     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3704
 
SphI G_CATG'C

Cuts at:    757    1257     757 
   Size:        500    3204
 
StyI C'CwwG_G

Cuts at:    107     838    1192     107 
   Size:        731     354    2619

  Fragments arranged by size: 

                2619     731     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1287    1420    1879    2088    1287 
   Size:        133     459     209    2903

  Fragments arranged by size: 

                2903     459     209     133
 
TatI w'GTAC_w

Cuts at:   3219    3219 
   Size:       3704
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3221    3253    3221 
   Size:         32    3672
 
Tth111I GACn'n_nGTC

Cuts at:   1501    1501 
   Size:       3704
 
XbaI T'CTAG_A

Cuts at:    735     735 
   Size:       3704
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    408    1199     408 
   Size:        791    2913
 
XmaI C'CCGG_G

Cuts at:    167     724     167 
   Size:        557    3147
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI    PflMI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI    BmgBI
     BmtI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI    BsmBI    BsrGI
   BssHII  BstZ17I   Bsu36I     ClaI    CspCI    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PfoI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
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