Restriction map for pF1KE2356
 (Linear) (Six-base) MAPSORT of: KIEE2356.seq  Check: 1760  from: 1  to: 8175



KIEE2356.orf

 With 182 enzymes: SgfI * 

                        December 2, 2013 11:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4422    5746    8175 
   Size:       4422    1324    2429

  Fragments arranged by size: 

                4422    2429    1324
 
AatII G_ACGT'C

Cuts at:      0    4256    8175 
   Size:       4256    3919
 
AccI GT'mk_AC

Cuts at:      0     935    8175 
   Size:        935    7240
 
Acc65I G'GTAC_C

Cuts at:      0    5098    8175 
   Size:       5098    3077
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     971    2125    5255    5920    5998    8175 
   Size:        400     571    1154    3130     665      78    2177

  Fragments arranged by size: 

                3130    2177    1154     665     571     400      78
 
AflIII A'CryG_T

Cuts at:      0     296    5891    8175 
   Size:        296    5595    2284

  Fragments arranged by size: 

                5595    2284     296
 
AgeI A'CCGG_T

Cuts at:      0    7558    8175 
   Size:       7558     617
 
AleI CACnn'nnGTG

Cuts at:      0    1798    2641    3907    4456    5890    8175 
   Size:       1798     843    1266     549    1434    2285

  Fragments arranged by size: 

                2285    1798    1434    1266     843     549
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     889    5803    8175 
   Size:        889    4914    2372

  Fragments arranged by size: 

                4914    2372     889
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1579    1611    8175 
   Size:       1579      32    6564

  Fragments arranged by size: 

                6564    1579      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     492    2443    3772    4525    6458    7010    8175 
   Size:        492    1951    1329     753    1933     552    1165

  Fragments arranged by size: 

                1951    1933    1329    1165     753     552     492
 
ApaI G_GGCC'C

Cuts at:      0    1832    2434    2623    3104    3304    4377    4450    4535 
   Size:       1832     602     189     481     200    1073      73      85

Cuts at:   4535    4966    5695    6854    7927    8175 
   Size:        431     729    1159    1073     248

  Fragments arranged by size: 

                1832    1159    1073    1073     729     602     481     431
                 248     200     189      85      73
 
ApaLI G'TGCA_C

Cuts at:      0    1918    7330    8014    8175 
   Size:       1918    5412     684     161

  Fragments arranged by size: 

                5412    1918     684     161
 
ApoI r'AATT_y

Cuts at:      0    3709    8175 
   Size:       3709    4466
 
AvaI C'yCGr_G

Cuts at:      0     220     310     319     653    2290    3207    3462    3885 
   Size:        220      90       9     334    1637     917     255     423

Cuts at:   3885    4376    4741    5125    5415    5811    5952    7016    7307 
   Size:        491     365     384     290     396     141    1064     291

Cuts at:   7307    7325    7341    7543    7602    7919    8175 
   Size:         18      16     202      59     317     256

  Fragments arranged by size: 

                1637    1064     917     491     423     396     384     365
                 334     317     291     290     256     255     220     202
                 141      90      59      18      16       9
 
BamHI G'GATC_C

Cuts at:      0     503    6694    8175 
   Size:        503    6191    1481

  Fragments arranged by size: 

                6191    1481     503
 
BanI G'GyrC_C

Cuts at:      0     516     574     690     793    1366    1507    1620    1792 
   Size:        516      58     116     103     573     141     113     172

Cuts at:   1792    2170    2880    3138    3232    3628    3664    3928    4135 
   Size:        378     710     258      94     396      36     264     207

Cuts at:   4135    4422    4495    4569    4951    5098    5278    5316    5458 
   Size:        287      73      74     382     147     180      38     142

Cuts at:   5458    5734    5878    6316    6429    6937    7194    7303    7450 
   Size:        276     144     438     113     508     257     109     147

Cuts at:   7450    7990    8175 
   Size:        540     185

  Fragments arranged by size: 

                 710     573     540     516     508     438     396     382
                 378     287     276     264     258     257     207     185
                 180     172     147     147     144     142     141     116
                 113     113     109     103      94      74      73      58
                  38      36
 
BanII G_rGCy'C

Cuts at:      0     203    1526    1699    1832    2257    2351    2434    2623 
   Size:        203    1323     173     133     425      94      83     189

Cuts at:   2623    2863    3104    3304    4015    4294    4377    4450    4535 
   Size:        240     241     200     711     279      83      73      85

Cuts at:   4535    4966    5069    5231    5393    5545    5695    5821    5969 
   Size:        431     103     162     162     152     150     126     148

Cuts at:   5969    6314    6854    7262    7544    7867    7927    8175 
   Size:        345     540     408     282     323      60     248

  Fragments arranged by size: 

                1323     711     540     431     425     408     345     323
                 282     279     248     241     240     203     200     189
                 173     162     162     152     150     148     133     126
                 103      94      85      83      83      73      60
 
BbeI G_GCGC'C

Cuts at:      0     578    4426    5282    8175 
   Size:        578    3848     856    2893

  Fragments arranged by size: 

                3848    2893     856     578
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2332    5428    8175 
   Size:       2332    3096    2747

  Fragments arranged by size: 

                3096    2747    2332
 
BbvCI CC'TCA_GC

Cuts at:      0     190    2896    3407    5344    8175 
   Size:        190    2706     511    1937    2831

  Fragments arranged by size: 

                2831    2706    1937     511     190
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     345     379    3453    3487    4899    4933    8175 
   Size:        345      34    3074      34    1412      34    3242

  Fragments arranged by size: 

                3242    3074    1412     345      34      34      34
 
BclI T'GATC_A

Cuts at:      0    4994    8175 
   Size:       4994    3181
 
BglI GCCn_nnn'nGGC

Cuts at:      0     658    1757    2695    5994    6773    8175 
   Size:        658    1099     938    3299     779    1402

  Fragments arranged by size: 

                3299    1402    1099     938     779     658
 
BglII A'GATC_T

Cuts at:      0    4730    8175 
   Size:       4730    3445
 
BlpI GC'TnA_GC

Cuts at:      0     229    8175 
   Size:        229    7946
 
Bme1580I G_kGCm'C

Cuts at:      0     521     695     798    1025    1371    1555    1832    1922 
   Size:        521     174     103     227     346     184     277      90

Cuts at:   1922    2195    2249    2434    2623    3104    3304    3707    4007 
   Size:        273      54     185     189     481     200     403     300

Cuts at:   4007    4138    4377    4450    4535    4966    5695    5881    5949 
   Size:        131     239      73      85     431     729     186      68

Cuts at:   5949    6839    6854    6893    6942    7308    7334    7455    7927 
   Size:        890      15      39      49     366      26     121     472

Cuts at:   7927    8018    8175 
   Size:         91     157

  Fragments arranged by size: 

                 890     729     521     481     472     431     403     366
                 346     300     277     273     239     227     200     189
                 186     185     184     174     157     131     121     103
                  91      90      85      73      68      54      49      39
                  26      15
 
BmgBI CAC'GTC

Cuts at:      0    1923    2394    5892    8175 
   Size:       1923     471    3498    2283

  Fragments arranged by size: 

                3498    2283    1923     471
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     624     759    1819    4907    5304    8175 
   Size:        624     135    1060    3088     397    2871

  Fragments arranged by size: 

                3088    2871    1060     624     397     135
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1848    4185    5247    6753    8175 
   Size:       1848    2337    1062    1506    1422

  Fragments arranged by size: 

                2337    1848    1506    1422    1062
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310    1303    2050    3064    3514    4099    4262    5681 
   Size:        310     993     747    1014     450     585     163    1419

Cuts at:   5681    5789    6991    7231    7851    8175 
   Size:        108    1202     240     620     324

  Fragments arranged by size: 

                1419    1202    1014     993     747     620     585     450
                 324     310     240     163     108
 
Bpu10I CC'TnA_GC

Cuts at:      0     190     971    2896    3407    4344    5344    5605    8175 
   Size:        190     781    1925     511     937    1000     261    2570

  Fragments arranged by size: 

                2570    1925    1000     937     781     511     261     190
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133    1510    2320    3199    6457    7246    7839    8175 
   Size:        133    1377     810     879    3258     789     593     336

  Fragments arranged by size: 

                3258    1377     879     810     789     593     336     133
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     607    2093    3217    3768    4114    7152    8175 
   Size:        607    1486    1124     551     346    3038    1023

  Fragments arranged by size: 

                3038    1486    1124    1023     607     551     346
 
BsaAI yAC'GTr

Cuts at:      0    1649    2190    3861    4790    5193    6039    6354    6888 
   Size:       1649     541    1671     929     403     846     315     534

Cuts at:   6888    8049    8175 
   Size:       1161     126

  Fragments arranged by size: 

                1671    1649    1161     929     846     541     534     403
                 315     126
 
BsaBI GATnn'nnATC

Cuts at:      0    5509    8175 
   Size:       5509    2666
 
BsaHI Gr'CG_yC

Cuts at:      0      26     478     575    2123    3755    4253    4423    5279 
   Size:         26     452      97    1548    1632     498     170     856

Cuts at:   5279    6119    7109    7421    8175 
   Size:        840     990     312     754

  Fragments arranged by size: 

                1632    1548     990     856     840     754     498     452
                 312     170      97      26
 
BsaWI w'CCGG_w

Cuts at:      0    7558    7792    8175 
   Size:       7558     234     383

  Fragments arranged by size: 

                7558     383     234
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     283     313     412     442    3970    4000    7674    7704 
   Size:        283      30      99      30    3528      30    3674      30

Cuts at:   7704    8175 
   Size:        471

  Fragments arranged by size: 

                3674    3528     471     283      99      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     727     979    1573    3109    3973    5236    5239    5255 
   Size:        727     252     594    1536     864    1263       3      16

Cuts at:   5255    5320    5977    6637    7369    7842    7884    8053    8175 
   Size:         65     657     660     732     473      42     169     122

  Fragments arranged by size: 

                1536    1263     864     732     727     660     657     594
                 473     252     169     122      65      42      16       3
            
 
BseYI C'CCAG_C

Cuts at:      0     521    1033    1582    4356    4443    4518    4602    6924 
   Size:        521     512     549    2774      87      75      84    2322

Cuts at:   6924    7030    7504    7739    8175 
   Size:        106     474     235     436

  Fragments arranged by size: 

                2774    2322     549     521     512     474     436     235
                 106      87      84      75
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     919    1296    2105    2731    3751    3961    4197 
   Size:        400     519     377     809     626    1020     210     236

Cuts at:   4197    5398    5482    5724    6737    7312    7663    8175 
   Size:       1201      84     242    1013     575     351     512

  Fragments arranged by size: 

                1201    1020    1013     809     626     575     519     512
                 400     377     351     242     236     210      84
 
BsiEI CG_ry'CG

Cuts at:      0    3743    6863    8175 
   Size:       3743    3120    1312

  Fragments arranged by size: 

                3743    3120    1312
 
BsiHKAI G_wGCw'C

Cuts at:      0     146     230     332    1058    1526    1922    3878    4345 
   Size:        146      84     102     726     468     396    1956     467

Cuts at:   4345    5069    5171    5393    6038    7172    7334    8018    8175 
   Size:        724     102     222     645    1134     162     684     157

  Fragments arranged by size: 

                1956    1134     726     724     684     645     468     467
                 396     222     162     157     146     102     102      84
            
 
BsmI GAATG_Cn'

Cuts at:      0     741    2953    7599    8175 
   Size:        741    2212    4646     576

  Fragments arranged by size: 

                4646    2212     741     576
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    4981    7116    7234    7246    7371    8175 
   Size:       4981    2135     118      12     125     804

  Fragments arranged by size: 

                4981    2135     804     125     118      12
 
Bsp1286I G_dGCh'C

Cuts at:      0     146     203     230     332     521     695     798    1025 
   Size:        146      57      27     102     189     174     103     227

Cuts at:   1025    1058    1371    1526    1555    1699    1832    1922    2195 
   Size:         33     313     155      29     144     133      90     273

Cuts at:   2195    2249    2257    2351    2434    2623    2863    3104    3304 
   Size:         54       8      94      83     189     240     241     200

Cuts at:   3304    3707    3878    4007    4015    4138    4294    4345    4377 
   Size:        403     171     129       8     123     156      51      32

Cuts at:   4377    4450    4535    4966    5069    5171    5231    5393    5545 
   Size:         73      85     431     103     102      60     162     152

Cuts at:   5545    5695    5821    5881    5949    5969    6038    6314    6839 
   Size:        150     126      60      68      20      69     276     525

Cuts at:   6839    6854    6893    6942    7172    7262    7308    7334    7455 
   Size:         15      39      49     230      90      46      26     121

Cuts at:   7455    7544    7867    7927    8018    8175 
   Size:         89     323      60      91     157

  Fragments arranged by size: 

                 525     431     403     323     313     276     273     241
                 240     230     227     200     189     189     174     171
                 162     157     156     155     152     150     146     144
                 133     129     126     123     121     103     103     102
                 102      94      91      90      90      89      85      83
                  73      69      68      60      60      60      57      54
                  51      49      46      39      33      32      29      27
                  26      20      15       8       8
 
BspEI T'CCGG_A

Cuts at:      0    7792    8175 
   Size:       7792     383
 
BspHI T'CATG_A

Cuts at:      0    4608    8175 
   Size:       4608    3567
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     185    1332    3371    4422    4814    5746    6196    8175 
   Size:        185    1147    2039    1051     392     932     450    1979

  Fragments arranged by size: 

                2039    1979    1147    1051     932     450     392     185
            
 
BsrBI CCG'CTC

Cuts at:      0    2295    3061    4132    8175 
   Size:       2295     766    1071    4043

  Fragments arranged by size: 

                4043    2295    1071     766
 
BsrDI GCAATG_nn'

Cuts at:      0     144     701    1173    1961    6207    7043    7715    8175 
   Size:        144     557     472     788    4246     836     672     460

  Fragments arranged by size: 

                4246     836     788     672     557     472     460     144
            
 
BsrFI r'CCGG_y

Cuts at:      0      28    1213    1256    1510    2038    2689    3023    4823 
   Size:         28    1185      43     254     528     651     334    1800

Cuts at:   4823    5017    5993    6385    6985    7558    7597    8175 
   Size:        194     976     392     600     573      39     578

  Fragments arranged by size: 

                1800    1185     976     651     600     578     573     528
                 392     334     254     194      43      39      28
 
BsrGI T'GTAC_A

Cuts at:      0    8072    8175 
   Size:       8072     103
 
BssSI C'ACGA_G

Cuts at:      0     340    2081    2969    3869    5218    7411    8175 
   Size:        340    1741     888     900    1349    2193     764

  Fragments arranged by size: 

                2193    1741    1349     900     888     764     340
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     492    2411    3702    8175 
   Size:        492    1919    1291    4473

  Fragments arranged by size: 

                4473    1919    1291     492
 
BstEII G'GTnAC_C

Cuts at:      0    1047    1638    3130    3804    6381    6759    8175 
   Size:       1047     591    1492     674    2577     378    1416

  Fragments arranged by size: 

                2577    1492    1416    1047     674     591     378
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3334    3421    4458    5536    6488    7050    7417    7462 
   Size:       3334      87    1037    1078     952     562     367      45

Cuts at:   7462    7827    7911    8175 
   Size:        365      84     264

  Fragments arranged by size: 

                3334    1078    1037     952     562     367     365     264
                  87      84      45
 
BstYI r'GATC_y

Cuts at:      0     107     503    2552    3572    3832    4730    4859    6005 
   Size:        107     396    2049    1020     260     898     129    1146

Cuts at:   6005    6089    6476    6694    7478    8175 
   Size:         84     387     218     784     697

  Fragments arranged by size: 

                2049    1146    1020     898     784     697     396     387
                 260     218     129     107      84
 
Bsu36I CC'TnA_GG

Cuts at:      0     710    7667    8175 
   Size:        710    6957     508

  Fragments arranged by size: 

                6957     710     508
 
BtgI C'CryG_G

Cuts at:      0      68      97     302     641    1140    1328    1895    3368 
   Size:         68      29     205     339     499     188     567    1473

Cuts at:   3368    4217    4941    5610    5684    5885    6876    7694    7820 
   Size:        849     724     669      74     201     991     818     126

Cuts at:   7820    7970    8175 
   Size:        150     205

  Fragments arranged by size: 

                1473     991     849     818     724     669     567     499
                 339     205     205     201     188     150     126      74
                  68      29
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      59     195     819    1238    1892    6423    8175 
   Size:         59     136     624     419     654    4531    1752

  Fragments arranged by size: 

                4531    1752     654     624     419     136      59
 
BtsI GCAGTG_nn'

Cuts at:      0    1202    2828    8175 
   Size:       1202    1626    5347

  Fragments arranged by size: 

                5347    1626    1202
 
ClaI AT'CG_AT

Cuts at:      0    7433    8175 
   Size:       7433     742
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1173    1208    6230    6265    6858    6893    7713    7748 
   Size:       1173      35    5022      35     593      35     820      35

Cuts at:   7748    8175 
   Size:        427

  Fragments arranged by size: 

                5022    1173     820     593     427      35      35      35
                  35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1047    3138    3790    6042    6948    7749    8175 
   Size:       1047    2091     652    2252     906     801     426

  Fragments arranged by size: 

                2252    2091    1047     906     801     652     426
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     759    4252    7144    8140    8175 
   Size:        759    3493    2892     996      35

  Fragments arranged by size: 

                3493    2892     996     759      35
 
EaeI y'GGCC_r

Cuts at:      0    1065    1172    1749    4416    4460    4821    5681    6879 
   Size:       1065     107     577    2667      44     361     860    1198

Cuts at:   6879    6983    8175 
   Size:        104    1192

  Fragments arranged by size: 

                2667    1198    1192    1065     860     577     361     107
                 104      44
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     100     265    3571    6435    8175 
   Size:        100     165    3306    2864    1740

  Fragments arranged by size: 

                3306    2864    1740     165     100
 
EcoICRI GAG'CTC

Cuts at:      0    1524    5067    5391    8175 
   Size:       1524    3543     324    2784

  Fragments arranged by size: 

                3543    2784    1524     324
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     400     971    1303    2050    2125    3064    3514 
   Size:        310      90     571     332     747      75     939     450

Cuts at:   3514    4099    4262    5255    5681    5789    5920    5998    6991 
   Size:        585     163     993     426     108     131      78     993

Cuts at:   6991    7231    7851    8175 
   Size:        240     620     324

  Fragments arranged by size: 

                 993     993     939     747     620     585     571     450
                 426     332     324     310     240     163     131     108
                  90      78      75
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      85    3799    8175 
   Size:         85    3714    4376

  Fragments arranged by size: 

                4376    3714      85
 
EcoO109I rG'GnC_Cy

Cuts at:      0      78     627     945    1732    1828    1829    2431    2619 
   Size:         78     549     318     787      96       1     602     188

Cuts at:   2619    2620    2844    2916    3100    3101    3300    3301    3769 
   Size:          1     224      72     184       1     199       1     468

Cuts at:   3769    3981    4143    4165    4373    4447    4532    4596    4963 
   Size:        212     162      22     208      74      85      64     367

Cuts at:   4963    5348    5691    5858    6645    6850    6851    7035    7057 
   Size:        385     343     167     787     205       1     184      22

Cuts at:   7057    7509    7607    7923    7924    8169    8175 
   Size:        452      98     316       1     245       6

  Fragments arranged by size: 

                 787     787     602     549     468     452     385     367
                 343     318     316     245     224     212     208     205
                 199     188     184     184     167     162      98      96
                  85      78      74      72      64      22      22       6
                   1       1       1       1       1       1
 
EcoRV GAT'ATC

Cuts at:      0    3117    8175 
   Size:       3117    5058
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2274    2880    4032    7980    8175 
   Size:       2274     606    1152    3948     195

  Fragments arranged by size: 

                3948    2274    1152     606     195
 
FspI TGC'GCA

Cuts at:      0    6052    8175 
   Size:       6052    2123
 
HaeII r_GCGC'y

Cuts at:      0     168     578    4426    5125    5282    5985    6519    6690 
   Size:        168     410    3848     699     157     703     534     171

Cuts at:   6690    6818    7031    8175 
   Size:        128     213    1144

  Fragments arranged by size: 

                3848    1144     703     699     534     410     213     171
                 168     157     128
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      49      81     283     315    3562    3594    3817    3849 
   Size:         49      32     202      32    3247      32     223      32

Cuts at:   3849    5680    5712    7618    7650    8175 
   Size:       1831      32    1906      32     525

  Fragments arranged by size: 

                3247    1906    1831     525     223     202      49      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    4095    4257    5622    8175 
   Size:       4095     162    1365    2553

  Fragments arranged by size: 

                4095    2553    1365     162
 
KasI G'GCGC_C

Cuts at:      0     574    4422    5278    8175 
   Size:        574    3848     856    2897

  Fragments arranged by size: 

                3848    2897     856     574
 
KpnI G_GTAC'C

Cuts at:      0    5102    8175 
   Size:       5102    3073
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1385    3560    8175 
   Size:       1385    2175    4615

  Fragments arranged by size: 

                4615    2175    1385
 
MscI TGG'CCA

Cuts at:      0    1067    1174    1751    6881    8175 
   Size:       1067     107     577    5130    1294

  Fragments arranged by size: 

                5130    1294    1067     577     107
 
MslI CAynn'nnrTG

Cuts at:      0     430     436    1798    2149    2527    2530    2578    2641 
   Size:        430       6    1362     351     378       3      48      63

Cuts at:   2641    2821    3907    4456    5467    5585    5785    5890    7048 
   Size:        180    1086     549    1011     118     200     105    1158

Cuts at:   7048    7415    7996    8002    8175 
   Size:        367     581       6     173

  Fragments arranged by size: 

                1362    1158    1086    1011     581     549     430     378
                 367     351     200     180     173     118     105      63
                  48       6       6       3
 
MspA1I CmG'CkG

Cuts at:      0      14     489     525     816    1310    1517    2062    2089 
   Size:         14     475      36     291     494     207     545      27

Cuts at:   2089    2180    2800    2889    4634    5247    5868    5928    6924 
   Size:         91     620      89    1745     613     621      60     996

Cuts at:   6924    7296    7647    8175 
   Size:        372     351     528

  Fragments arranged by size: 

                1745     996     621     620     613     545     528     494
                 475     372     351     291     207      91      89      60
                  36      27      14
 
NaeI GCC'GGC

Cuts at:      0      30    1215    1258    1512    2691    3025    6987    7599 
   Size:         30    1185      43     254    1179     334    3962     612

Cuts at:   7599    8175 
   Size:        576

  Fragments arranged by size: 

                3962    1185    1179     612     576     334     254      43
                  30
 
NarI GG'CG_CC

Cuts at:      0     575    4423    5279    8175 
   Size:        575    3848     856    2896

  Fragments arranged by size: 

                3848    2896     856     575
 
NcoI C'CATG_G

Cuts at:      0    1328    4941    5610    7694    8175 
   Size:       1328    3613     669    2084     481

  Fragments arranged by size: 

                3613    2084    1328     669     481
 
NgoMIV G'CCGG_C

Cuts at:      0      28    1213    1256    1510    2689    3023    6985    7597 
   Size:         28    1185      43     254    1179     334    3962     612

Cuts at:   7597    8175 
   Size:        578

  Fragments arranged by size: 

                3962    1185    1179     612     578     334     254      43
                  28
 
NspI r_CATG'y

Cuts at:      0     246    1354    3087    5039    6070    6287    8175 
   Size:        246    1108    1733    1952    1031     217    1888

  Fragments arranged by size: 

                1952    1888    1733    1108    1031     246     217
 
PasI CC'CwG_GG

Cuts at:      0    1113    2623    4285    4327    5536    7927    8175 
   Size:       1113    1510    1662      42    1209    2391     248

  Fragments arranged by size: 

                2391    1662    1510    1209    1113     248      42
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     642    1649    3055    4734    4947    5196    5294    5616 
   Size:        642    1007    1406    1679     213     249      98     322

Cuts at:   5616    6846    8175 
   Size:       1230    1329

  Fragments arranged by size: 

                1679    1406    1329    1230    1007     642     322     249
                 213      98
 
PfoI T'CCnGG_A

Cuts at:      0    4239    4769    7124    7247    8175 
   Size:       4239     530    2355     123     928

  Fragments arranged by size: 

                4239    2355     928     530     123
 
PmlI CAC'GTG

Cuts at:      0    1649    3861    5193    6039    6354    6888    8175 
   Size:       1649    2212    1332     846     315     534    1287

  Fragments arranged by size: 

                2212    1649    1332    1287     846     534     315
 
PpuMI rG'GwC_Cy

Cuts at:      0      78     627     945    1732    2844    3981    4165    4596 
   Size:         78     549     318     787    1112    1137     184     431

Cuts at:   4596    5858    6645    7057    7509    8169    8175 
   Size:       1262     787     412     452     660       6

  Fragments arranged by size: 

                1262    1137    1112     787     787     660     549     452
                 431     412     318     184      78       6
 
PshAI GACnn'nnGTC

Cuts at:      0    2132    4406    8175 
   Size:       2132    2274    3769

  Fragments arranged by size: 

                3769    2274    2132
 
PspOMI G'GGCC_C

Cuts at:      0    1828    2430    2619    3100    3300    4373    4446    4531 
   Size:       1828     602     189     481     200    1073      73      85

Cuts at:   4531    4962    5691    6850    7923    8175 
   Size:        431     729    1159    1073     252

  Fragments arranged by size: 

                1828    1159    1073    1073     729     602     481     431
                 252     200     189      85      73
 
PspXI vC'TCGA_Gb

Cuts at:      0     220     310     319    7543    8175 
   Size:        220      90       9    7224     632

  Fragments arranged by size: 

                7224     632     220      90       9
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     238     270    8056    8088    8175 
   Size:        238      32    7786      32      87

  Fragments arranged by size: 

                7786     238      87      32      32
 
PstI C_TGCA'G

Cuts at:      0     182     881    1454    2374    2439    4408    4433    7981 
   Size:        182     699     573     920      65    1969      25    3548

Cuts at:   7981    8175 
   Size:        194

  Fragments arranged by size: 

                3548    1969     920     699     573     194     182      65
                  25
 
PvuII CAG'CTG

Cuts at:      0     525     816    2089    5247    5868    5928    7296    7647 
   Size:        525     291    1273    3158     621      60    1368     351

Cuts at:   7647    8175 
   Size:        528

  Fragments arranged by size: 

                3158    1368    1273     621     528     525     351     291
                  60
 
SacI G_AGCT'C

Cuts at:      0    1526    5069    5393    8175 
   Size:       1526    3543     324    2782

  Fragments arranged by size: 

                3543    2782    1526     324
 
SanDI GG'GwC_CC

Cuts at:      0     627    2844    4165    6645    7509    8175 
   Size:        627    2217    1321    2480     864     666

  Fragments arranged by size: 

                2480    2217    1321     864     666     627
 
SbfI CC_TGCA'GG

Cuts at:      0    4433    8175 
   Size:       4433    3742
 
ScaI AGT'ACT

Cuts at:      0    1609    8175 
   Size:       1609    6566
 
SexAI A'CCwGG_T

Cuts at:      0     789    5357    7131    8175 
   Size:        789    4568    1774    1044

  Fragments arranged by size: 

                4568    1774    1044     789
 
SfcI C'TryA_G

Cuts at:      0     178     877    1450    2370    2435    3645    4404    4429 
   Size:        178     699     573     920      65    1210     759      25

Cuts at:   4429    5775    6819    7872    7977    8175 
   Size:       1346    1044    1053     105     198

  Fragments arranged by size: 

                1346    1210    1053    1044     920     759     699     573
                 198     178     105      65      25
 
SfoI GGC'GCC

Cuts at:      0     576    4424    5280    8175 
   Size:        576    3848     856    2895

  Fragments arranged by size: 

                3848    2895     856     576
 
SmaI CCC'GGG

Cuts at:      0     655    3209    4378    5813    7018    7309    7327    8175 
   Size:        655    2554    1169    1435    1205     291      18     848

  Fragments arranged by size: 

                2554    1435    1205    1169     848     655     291      18
            
 
SmlI C'TyrA_G

Cuts at:      0     148     220     310     319    1525    2335    3214    6472 
   Size:        148      72      90       9    1206     810     879    3258

Cuts at:   6472    7261    7543    7854    8175 
   Size:        789     282     311     321

  Fragments arranged by size: 

                3258    1206     879     810     789     321     311     282
                 148      90      72       9
 
StuI AGG'CCT

Cuts at:      0     622    1448    2014    2333    4076    4643    5495    5603 
   Size:        622     826     566     319    1743     567     852     108

Cuts at:   5603    7942    8175 
   Size:       2339     233

  Fragments arranged by size: 

                2339    1743     852     826     622     567     566     319
                 233     108
 
StyI C'CwwG_G

Cuts at:      0     773    1025    1328    1457    1502    2681    2729    3188 
   Size:        773     252     303     129      45    1179      48     459

Cuts at:   3188    3260    4146    4941    5009    5610    5699    6020    7637 
   Size:         72     886     795      68     601      89     321    1617

Cuts at:   7637    7694    7769    8175 
   Size:         57      75     406

  Fragments arranged by size: 

                1617    1179     886     795     773     601     459     406
                 321     303     252     129      89      75      72      68
                  57      48      45
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     187    2677    4048    4513    4939    5496    6646    6916 
   Size:        187    2490    1371     465     426     557    1150     270

Cuts at:   6916    8175 
   Size:       1259

  Fragments arranged by size: 

                2490    1371    1259    1150     557     465     426     270
                 187
 
TatI w'GTAC_w

Cuts at:      0    1607    2495    2792    3383    3944    4223    8072    8175 
   Size:       1607     888     297     591     561     279    3849     103

  Fragments arranged by size: 

                3849    1607     888     591     561     297     279     103
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      82     114    1368    1400    3776    3808    7674    7706 
   Size:         82      32    1254      32    2376      32    3866      32

Cuts at:   7706    7801    7833    8175 
   Size:         95      32     342

  Fragments arranged by size: 

                3866    2376    1254     342      95      82      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2848    7845    8175 
   Size:       2848    4997     330

  Fragments arranged by size: 

                4997    2848     330
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     639    3528    4674    7644    7827    8001    8175 
   Size:        639    2889    1146    2970     183     174     174

  Fragments arranged by size: 

                2970    2889    1146     639     183     174     174
 
XhoI C'TCGA_G

Cuts at:      0     220     310     319    7543    8175 
   Size:        220      90       9    7224     632

  Fragments arranged by size: 

                7224     632     220      90       9
 
XmaI C'CCGG_G

Cuts at:      0     653    3207    4376    5811    7016    7307    7325    8175 
   Size:        653    2554    1169    1435    1205     291      18     850

  Fragments arranged by size: 

                2554    1435    1205    1169     850     653     291      18
            
 
XmnI GAAnn'nnTTC

Cuts at:      0     121    7963    8175 
   Size:        121    7842     212

  Fragments arranged by size: 

                7842     212     121
 
ZraI GAC'GTC

Cuts at:      0    4254    8175 
   Size:       4254    3921
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI   AflIII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EaeI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI    PvuII     SacI    SanDI     SbfI
     ScaI    SexAI     SfcI     SfoI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AclI     AfeI    AflII     AhdI     AscI     AseI    AsiSI    AvrII
     BaeI    BciVI    BfrBI     BmtI    BsiWI   BssHII    BstBI  BstZ17I
     DraI     EagI     EarI    EcoRI     FseI    FspAI  HindIII     HpaI
     MfeI     MluI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PciI     PmeI     PpiI     PsiI     PvuI    RsrII    SacII     SalI
     SapI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     XbaI
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