Restriction map for pF1KE2413
 (Linear) (Six-base) MAPSORT of: KIEE2413.seq  Check: 952  from: 1  to: 5931



KIEE2413.orf

 With 182 enzymes: SgfI * 

                        July 23, 2012 18:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     375    3091    5394    5931 
   Size:        375    2716    2303     537

  Fragments arranged by size: 

                2716    2303     537     375
 
AatII G_ACGT'C

Cuts at:      0    1350    5931 
   Size:       1350    4581
 
AccI GT'mk_AC

Cuts at:      0    3137    3356    5931 
   Size:       3137     219    2575

  Fragments arranged by size: 

                3137    2575     219
 
Acc65I G'GTAC_C

Cuts at:      0    2526    5355    5588    5931 
   Size:       2526    2829     233     343

  Fragments arranged by size: 

                2829    2526     343     233
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32    1307    3836    4045    4624    4834    5931 
   Size:         32    1275    2529     209     579     210    1097

  Fragments arranged by size: 

                2529    1275    1097     579     210     209      32
 
AflIII A'CryG_T

Cuts at:      0    1625    2084    2252    3152    3764    5931 
   Size:       1625     459     168     900     612    2167

  Fragments arranged by size: 

                2167    1625     900     612     459     168
 
AgeI A'CCGG_T

Cuts at:      0    1423    5931 
   Size:       1423    4508
 
AhdI GACnn_n'nnGTC

Cuts at:      0    5865    5931 
   Size:       5865      66
 
AleI CACnn'nnGTG

Cuts at:      0    1828    5931 
   Size:       1828    4103
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     234    1882    3070    5789    5931 
   Size:        234    1648    1188    2719     142

  Fragments arranged by size: 

                2719    1648    1188     234     142
 
AlwNI CAG_nnn'CTG

Cuts at:      0      64    1520    1913    2121    2302    4647    4692    5421 
   Size:         64    1456     393     208     181    2345      45     729

Cuts at:   5421    5931 
   Size:        510

  Fragments arranged by size: 

                2345    1456     729     510     393     208     181      64
                  45
 
ApaI G_GGCC'C

Cuts at:      0      95     550     917    1753    4366    5084    5492    5931 
   Size:         95     455     367     836    2613     718     408     439

  Fragments arranged by size: 

                2613     836     718     455     439     408     367      95
            
 
ApaLI G'TGCA_C

Cuts at:      0     873    5931 
   Size:        873    5058
 
ApoI r'AATT_y

Cuts at:      0    3073    4381    4744    4783    5199    5481    5931 
   Size:       3073    1308     363      39     416     282     450

  Fragments arranged by size: 

                3073    1308     450     416     363     282      39
 
AvaI C'yCGr_G

Cuts at:      0      88     206     660     766    5735    5931 
   Size:         88     118     454     106    4969     196

  Fragments arranged by size: 

                4969     454     196     118     106      88
 
AvrII C'CTAG_G

Cuts at:      0     132    5931 
   Size:        132    5799
 
BamHI G'GATC_C

Cuts at:      0     811    3935    5140    5931 
   Size:        811    3124    1205     791

  Fragments arranged by size: 

                3124    1205     811     791
 
BanI G'GyrC_C

Cuts at:      0     702    1840    2367    2526    5348    5355    5461    5588 
   Size:        702    1138     527     159    2822       7     106     127

Cuts at:   5588    5931 
   Size:        343

  Fragments arranged by size: 

                2822    1138     702     527     343     159     127     106
                   7
 
BanII G_rGCy'C

Cuts at:      0      41      95     550     767     917    1473    1753    2688 
   Size:         41      54     455     217     150     556     280     935

Cuts at:   2688    3127    3850    4366    5023    5084    5172    5331    5492 
   Size:        439     723     516     657      61      88     159     161

Cuts at:   5492    5502    5931 
   Size:         10     429

  Fragments arranged by size: 

                 935     723     657     556     516     455     439     429
                 280     217     161     159     150      88      61      54
                  41      10
 
BbeI G_GCGC'C

Cuts at:      0    1844    5931 
   Size:       1844    4087
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     845     960    1202    1608    1760    2607    3675    5931 
   Size:        845     115     242     406     152     847    1068    2256

  Fragments arranged by size: 

                2256    1068     847     845     406     242     152     115
            
 
BbvCI CC'TCA_GC

Cuts at:      0    2560    2578    2647    2669    5007    5525    5931 
   Size:       2560      18      69      22    2338     518     406

  Fragments arranged by size: 

                2560    2338     518     406      69      22      18
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     233     243     267     277    3277    3311    3523    3557 
   Size:        233      10      24      10    3000      34     212      34

Cuts at:   3557    4493    4527    5931 
   Size:        936      34    1404

  Fragments arranged by size: 

                3000    1404     936     233     212      34      34      34
                  24      10      10
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    4474    5931 
   Size:       4474    1457
 
BclI T'GATC_A

Cuts at:      0    4292    4316    5931 
   Size:       4292      24    1615

  Fragments arranged by size: 

                4292    1615      24
 
BfrBI ATG'CAT

Cuts at:      0    3292    5931 
   Size:       3292    2639
 
BglI GCCn_nnn'nGGC

Cuts at:      0      55     642    1301    1665    3229    3496    3839    4852 
   Size:         55     587     659     364    1564     267     343    1013

Cuts at:   4852    5931 
   Size:       1079

  Fragments arranged by size: 

                1564    1079    1013     659     587     364     343     267
                  55
 
BglII A'GATC_T

Cuts at:      0    3335    4667    5931 
   Size:       3335    1332    1264

  Fragments arranged by size: 

                3335    1332    1264
 
Bme1580I G_kGCm'C

Cuts at:      0      88      95     195     213     550     877     917    1560 
   Size:         88       7     100      18     337     327      40     643

Cuts at:   1560    1753    3426    4366    4498    5084    5132    5351    5492 
   Size:        193    1673     940     132     586      48     219     141

Cuts at:   5492    5931 
   Size:        439

  Fragments arranged by size: 

                1673     940     643     586     439     337     327     219
                 193     141     132     100      88      48      40      18
                   7
 
BmgBI CAC'GTC

Cuts at:      0     797    3153    5931 
   Size:        797    2356    2778

  Fragments arranged by size: 

                2778    2356     797
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      35    1605    1923    5078    5117    5931 
   Size:         35    1570     318    3155      39     814

  Fragments arranged by size: 

                3155    1570     814     318      39      35
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     372    1272    3876    5931 
   Size:        372     900    2604    2055

  Fragments arranged by size: 

                2604    2055     900     372
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     559    2859    4648    5097    5931 
   Size:        400     159    2300    1789     449     834

  Fragments arranged by size: 

                2300    1789     834     449     400     159
 
Bpu10I CC'TnA_GC

Cuts at:      0     143    2560    2578    2647    2669    5007    5501    5525 
   Size:        143    2417      18      69      22    2338     494      24

Cuts at:   5525    5931 
   Size:        406

  Fragments arranged by size: 

                2417    2338     494     406     143      69      24      22
                  18
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415    1211    2941    3095    3478    5931 
   Size:        415     796    1730     154     383    2453

  Fragments arranged by size: 

                2453    1730     796     415     383     154
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1432    1813    5001    5732    5931 
   Size:       1432     381    3188     731     199

  Fragments arranged by size: 

                3188    1432     731     381     199
 
BsaAI yAC'GTr

Cuts at:      0     396    5637    5931 
   Size:        396    5241     294

  Fragments arranged by size: 

                5241     396     294
 
BsaBI GATnn'nnATC

Cuts at:      0    3388    4657    5931 
   Size:       3388    1269    1274

  Fragments arranged by size: 

                3388    1274    1269
 
BsaHI Gr'CG_yC

Cuts at:      0     566    1106    1347    1841    4823    5931 
   Size:        566     540     241     494    2982    1108

  Fragments arranged by size: 

                2982    1108     566     540     494     241
 
BsaWI w'CCGG_w

Cuts at:      0    1423    2990    4592    5931 
   Size:       1423    1567    1602    1339

  Fragments arranged by size: 

                1602    1567    1423    1339
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2782    2812    5931 
   Size:       2782      30    3119

  Fragments arranged by size: 

                3119    2782      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     844    1474    1477    1876    1975    2092    2407    2440 
   Size:        844     630       3     399      99     117     315      33

Cuts at:   2440    2443    2446    2449    2452    4738    4741    4933    4936 
   Size:          3       3       3       3    2286       3     192       3

Cuts at:   4936    4966    5878    5905    5931 
   Size:         30     912      27      26

  Fragments arranged by size: 

                2286     912     844     630     399     315     192     117
                  99      33      30      27      26       3       3       3
                   3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0      96     194     463    1410    3500    5000    5659    5931 
   Size:         96      98     269     947    2090    1500     659     272

  Fragments arranged by size: 

                2090    1500     947     659     272     269      98      96
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     652     697    2498    2773    2989    3069    3076    3251 
   Size:        652      45    1801     275     216      80       7     175

Cuts at:   3251    5617    5931 
   Size:       2366     314

  Fragments arranged by size: 

                2366    1801     652     314     275     216     175      80
                  45       7
 
BsiHKAI G_wGCw'C

Cuts at:      0     431     458     680     734     877    1007    1649    1871 
   Size:        431      27     222      54     143     130     642     222

Cuts at:   1871    3716    3850    5305    5331    5931 
   Size:       1845     134    1455      26     600

  Fragments arranged by size: 

                1845    1455     642     600     431     222     222     143
                 134     130      54      27      26
 
BsmI GAATG_Cn'

Cuts at:      0     791    3121    5931 
   Size:        791    2330    2810

  Fragments arranged by size: 

                2810    2330     791
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     415    3143    4452    5897    5931 
   Size:        415    2728    1309    1445      34

  Fragments arranged by size: 

                2728    1445    1309     415      34
 
Bsp1286I G_dGCh'C

Cuts at:      0      41      88      95     195     213     431     458     550 
   Size:         41      47       7     100      18     218      27      92

Cuts at:    550     680     734     767     877     917    1007    1473    1560 
   Size:        130      54      33     110      40      90     466      87

Cuts at:   1560    1649    1753    1871    2688    3127    3426    3716    3850 
   Size:         89     104     118     817     439     299     290     134

Cuts at:   3850    4366    4498    5023    5084    5132    5172    5305    5331 
   Size:        516     132     525      61      48      40     133      26

Cuts at:   5331    5351    5492    5502    5931 
   Size:         20     141      10     429

  Fragments arranged by size: 

                 817     525     516     466     439     429     299     290
                 218     141     134     133     132     130     118     110
                 104     100      92      90      89      87      61      54
                  48      47      41      40      40      33      27      26
                  20      18      10       7
 
BspEI T'CCGG_A

Cuts at:      0    2990    4592    5931 
   Size:       2990    1602    1339

  Fragments arranged by size: 

                2990    1602    1339
 
BspHI T'CATG_A

Cuts at:      0     689    1505    3008    3380    5931 
   Size:        689     816    1503     372    2551

  Fragments arranged by size: 

                2551    1503     816     689     372
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     375    1208    3091    5380    5394    5834    5931 
   Size:        375     833    1883    2289      14     440      97

  Fragments arranged by size: 

                2289    1883     833     440     375      97      14
 
BsrBI CCG'CTC

Cuts at:      0    3177    5931 
   Size:       3177    2754
 
BsrDI GCAATG_nn'

Cuts at:      0     486     716    2264    2337    3368    3943    4010    4535 
   Size:        486     230    1548      73    1031     575      67     525

Cuts at:   4535    5931 
   Size:       1396

  Fragments arranged by size: 

                1548    1396    1031     575     525     486     230      73
                  67
 
BsrFI r'CCGG_y

Cuts at:      0    1423    5931 
   Size:       1423    4508
 
BsrGI T'GTAC_A

Cuts at:      0    1483    5931 
   Size:       1483    4448
 
BssSI C'ACGA_G

Cuts at:      0     727    5931 
   Size:        727    5204
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     675    2302    2967    5931 
   Size:        675    1627     665    2964

  Fragments arranged by size: 

                2964    1627     675     665
 
BstEII G'GTnAC_C

Cuts at:      0     200    1902    4731    5931 
   Size:        200    1702    2829    1200

  Fragments arranged by size: 

                2829    1702    1200     200
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     318    1830    2271    2889    5931 
   Size:        318    1512     441     618    3042

  Fragments arranged by size: 

                3042    1512     618     441     318
 
BstYI r'GATC_y

Cuts at:      0     811    1676    1892    2117    2340    2829    3335    3935 
   Size:        811     865     216     225     223     489     506     600

Cuts at:   3935    4391    4667    5140    5599    5931 
   Size:        456     276     473     459     332

  Fragments arranged by size: 

                 865     811     600     506     489     473     459     456
                 332     276     225     223     216
 
BstZ17I GTA'TAC

Cuts at:      0    3138    5931 
   Size:       3138    2793
 
Bsu36I CC'TnA_GG

Cuts at:      0     347    1888    2537    5931 
   Size:        347    1541     649    3394

  Fragments arranged by size: 

                3394    1541     649     347
 
BtgI C'CryG_G

Cuts at:      0     897     983    1025    1650    2793    3306    3662    4736 
   Size:        897      86      42     625    1143     513     356    1074

Cuts at:   4736    5664    5931 
   Size:        928     267

  Fragments arranged by size: 

                1143    1074     928     897     625     513     356     267
                  86      42
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     824    1463    1620    1950    3708    3984    4236    5931 
   Size:        824     639     157     330    1758     276     252    1695

  Fragments arranged by size: 

                1758    1695     824     639     330     276     252     157
            
 
BtsI GCAGTG_nn'

Cuts at:      0     123     188     362     721    1580    2300    2658    2977 
   Size:        123      65     174     359     859     720     358     319

Cuts at:   2977    3268    5611    5685    5931 
   Size:        291    2343      74     246

  Fragments arranged by size: 

                2343     859     720     359     358     319     291     246
                 174     123      74      65
 
ClaI AT'CG_AT

Cuts at:      0    3287    5931 
   Size:       3287    2644
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3810    3845    4563    4598    5931 
   Size:       3810      35     718      35    1333

  Fragments arranged by size: 

                3810    1333     718      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     675    4506    5931 
   Size:        675    3831    1425

  Fragments arranged by size: 

                3831    1425     675
 
EaeI y'GGCC_r

Cuts at:      0     185     230     556     634     907    1309    1319    1475 
   Size:        185      45     326      78     273     402      10     156

Cuts at:   1475    2282    2663    2984    3831    5759    5931 
   Size:        807     381     321     847    1928     172

  Fragments arranged by size: 

                1928     847     807     402     381     326     321     273
                 185     172     156      78      45      10
 
EarI CTCTTCn'nnn_

Cuts at:      0     220     739    1229    1283    1993    1999    2227    2320 
   Size:        220     519     490      54     710       6     228      93

Cuts at:   2320    2384    2441    2450    2459    2465    3067    3700    3895 
   Size:         64      57       9       9       6     602     633     195

Cuts at:   3895    3998    4064    4217    4845    4925    5561    5665    5931 
   Size:        103      66     153     628      80     636     104     266

  Fragments arranged by size: 

                 710     636     633     628     602     519     490     266
                 228     220     195     153     104     103      93      80
                  66      64      57      54       9       9       6       6
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1248    1522    3499    4806    4833    5931 
   Size:       1248     274    1977    1307      27    1098

  Fragments arranged by size: 

                1977    1307    1248    1098     274      27
 
EcoICRI GAG'CTC

Cuts at:      0    3848    5329    5931 
   Size:       3848    1481     602

  Fragments arranged by size: 

                3848    1481     602
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     400     559    1307    2859    3836    4045    4624 
   Size:         32     368     159     748    1552     977     209     579

Cuts at:   4624    4648    4834    5097    5931 
   Size:         24     186     263     834

  Fragments arranged by size: 

                1552     977     834     748     579     368     263     209
                 186     159      32      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     382    5931 
   Size:        382    5549
 
EcoO109I rG'GnC_Cy

Cuts at:      0      61      92     137     546     547     657     834     913 
   Size:         61      31      45     409       1     110     177      79

Cuts at:    913    1076    1251    1548    1750    2097    2673    2780    4363 
   Size:        163     175     297     202     347     576     107    1583

Cuts at:   4363    4882    4899    5488    5931 
   Size:        519      17     589     443

  Fragments arranged by size: 

                1583     589     576     519     443     409     347     297
                 202     177     175     163     110     107      79      61
                  45      31      17       1
 
EcoRI G'AATT_C

Cuts at:      0    4381    5931 
   Size:       4381    1550
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2064    3144    3762    5421    5931 
   Size:       2064    1080     618    1659     510

  Fragments arranged by size: 

                2064    1659    1080     618     510
 
FspI TGC'GCA

Cuts at:      0    5617    5931 
   Size:       5617     314
 
HaeII r_GCGC'y

Cuts at:      0     470     906    1844    2549    2882    5430    5931 
   Size:        470     436     938     705     333    2548     501

  Fragments arranged by size: 

                2548     938     705     501     470     436     333
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     320     352     823     855    4186    4218    4284    4316 
   Size:        320      32     471      32    3331      32      66      32

Cuts at:   4316    5056    5088    5851    5883    5931 
   Size:        740      32     763      32      48

  Fragments arranged by size: 

                3331     763     740     471     320      66      48      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1631    3189    5931 
   Size:       1631    1558    2742

  Fragments arranged by size: 

                2742    1631    1558
 
HindIII A'AGCT_T

Cuts at:      0    3913    5931 
   Size:       3913    2018
 
KasI G'GCGC_C

Cuts at:      0    1840    5931 
   Size:       1840    4091
 
KpnI G_GTAC'C

Cuts at:      0    2530    5359    5592    5931 
   Size:       2530    2829     233     339

  Fragments arranged by size: 

                2829    2530     339     233
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     839    2807    5168    5374    5931 
   Size:        383     456    1968    2361     206     557

  Fragments arranged by size: 

                2361    1968     557     456     383     206
 
MscI TGG'CCA

Cuts at:      0     187     232     909    1477    2284    2986    3833    5761 
   Size:        187      45     677     568     807     702     847    1928

Cuts at:   5761    5931 
   Size:        170

  Fragments arranged by size: 

                1928     847     807     702     677     568     187     170
                  45
 
MslI CAynn'nnrTG

Cuts at:      0     511    1828    1864    2269    2609    2887    3052    3394 
   Size:        511    1317      36     405     340     278     165     342

Cuts at:   3394    4003    4033    4039    4376    4439    5931 
   Size:        609      30       6     337      63    1492

  Fragments arranged by size: 

                1492    1317     609     511     405     342     340     337
                 278     165      63      36      30       6
 
MspA1I CmG'CkG

Cuts at:      0     100     198     708     899    1173    1317    1481    2028 
   Size:        100      98     510     191     274     144     164     547

Cuts at:   2028    2577    2668    2795    5529    5553    5578    5931 
   Size:        549      91     127    2734      24      25     353

  Fragments arranged by size: 

                2734     549     547     510     353     274     191     164
                 144     127     100      98      91      25      24
 
NarI GG'CG_CC

Cuts at:      0    1841    5931 
   Size:       1841    4090
 
NcoI C'CATG_G

Cuts at:      0     983    1025    3662    5664    5931 
   Size:        983      42    2637    2002     267

  Fragments arranged by size: 

                2637    2002     983     267      42
 
NdeI CA'TA_TG

Cuts at:      0    2169    3294    5931 
   Size:       2169    1125    2637

  Fragments arranged by size: 

                2637    2169    1125
 
NsiI A_TGCA'T

Cuts at:      0    3294    5931 
   Size:       3294    2637
 
NspI r_CATG'y

Cuts at:      0    2025    2965    4038    4539    5931 
   Size:       2025     940    1073     501    1392

  Fragments arranged by size: 

                2025    1392    1073     940     501
 
PasI CC'CwG_GG

Cuts at:      0     324    1043    3426    4411    5931 
   Size:        324     719    2383     985    1520

  Fragments arranged by size: 

                2383    1520     985     719     324
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      52     378    1913    2709    4349    5931 
   Size:         52     326    1535     796    1640    1582

  Fragments arranged by size: 

                1640    1582    1535     796     326      52
 
PfoI T'CCnGG_A

Cuts at:      0    1679    3170    4179    4766    5023    5931 
   Size:       1679    1491    1009     587     257     908

  Fragments arranged by size: 

                1679    1491    1009     908     587     257
 
PmlI CAC'GTG

Cuts at:      0    5637    5931 
   Size:       5637     294
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    5832    5864    5931 
   Size:       5832      32      67

  Fragments arranged by size: 

                5832      67      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     137     657     834    1076    1251    1548    2673    4882 
   Size:        137     520     177     242     175     297    1125    2209

Cuts at:   4882    5931 
   Size:       1049

  Fragments arranged by size: 

                2209    1125    1049     520     297     242     177     175
                 137
 
PshAI GACnn'nnGTC

Cuts at:      0    4880    5931 
   Size:       4880    1051
 
PspOMI G'GGCC_C

Cuts at:      0      91     546     913    1749    4362    5080    5488    5931 
   Size:         91     455     367     836    2613     718     408     443

  Fragments arranged by size: 

                2613     836     718     455     443     408     367      91
            
 
PspXI vC'TCGA_Gb

Cuts at:      0     766    5931 
   Size:        766    5165
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    3571    3603    5931 
   Size:       3571      32    2328

  Fragments arranged by size: 

                3571    2328      32
 
PstI C_TGCA'G

Cuts at:      0    2501    4874    5391    5426    5931 
   Size:       2501    2373     517      35     505

  Fragments arranged by size: 

                2501    2373     517     505      35
 
PvuII CAG'CTG

Cuts at:      0     100    1317    1481    2028    5931 
   Size:        100    1217     164     547    3903

  Fragments arranged by size: 

                3903    1217     547     164     100
 
SacI G_AGCT'C

Cuts at:      0    3850    5331    5931 
   Size:       3850    1481     600

  Fragments arranged by size: 

                3850    1481     600
 
SacII CC_GC'GG

Cuts at:      0     900    2796    5931 
   Size:        900    1896    3135

  Fragments arranged by size: 

                3135    1896     900
 
SanDI GG'GwC_CC

Cuts at:      0     137     657    5931 
   Size:        137     520    5274

  Fragments arranged by size: 

                5274     520     137
 
SapI GCTCTTCn'nnn_

Cuts at:      0     739    1229    2320    3067    3998    4064    5931 
   Size:        739     490    1091     747     931      66    1867

  Fragments arranged by size: 

                1867    1091     931     747     739     490      66
 
SbfI CC_TGCA'GG

Cuts at:      0    2501    4874    5391    5931 
   Size:       2501    2373     517     540

  Fragments arranged by size: 

                2501    2373     540     517
 
ScaI AGT'ACT

Cuts at:      0    2909    2918    5931 
   Size:       2909       9    3013

  Fragments arranged by size: 

                3013    2909       9
 
SexAI A'CCwGG_T

Cuts at:      0    5351    5931 
   Size:       5351     580
 
SfcI C'TryA_G

Cuts at:      0    2497    4870    5387    5422    5853    5931 
   Size:       2497    2373     517      35     431      78

  Fragments arranged by size: 

                2497    2373     517     431      78      35
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1301    5931 
   Size:       1301    4630
 
SfoI GGC'GCC

Cuts at:      0    1842    5931 
   Size:       1842    4089
 
SmaI CCC'GGG

Cuts at:      0      90    5931 
   Size:         90    5841
 
SmlI C'TyrA_G

Cuts at:      0     430     766    1226    2956    3110    3493    5735    5931 
   Size:        430     336     460    1730     154     383    2242     196

  Fragments arranged by size: 

                2242    1730     460     430     383     336     196     154
            
 
SnaBI TAC'GTA

Cuts at:      0     396    5931 
   Size:        396    5535
 
SphI G_CATG'C

Cuts at:      0    2025    4038    5931 
   Size:       2025    2013    1893

  Fragments arranged by size: 

                2025    2013    1893
 
StuI AGG'CCT

Cuts at:      0     131    2363    3976    5931 
   Size:        131    2232    1613    1955

  Fragments arranged by size: 

                2232    1955    1613     131
 
StyI C'CwwG_G

Cuts at:      0     132     777     983    1025    1127    1272    1443    1826 
   Size:        132     645     206      42     102     145     171     383

Cuts at:   1826    1853    2306    2513    2948    3662    3837    4418    5210 
   Size:         27     453     207     435     714     175     581     792

Cuts at:   5210    5664    5931 
   Size:        454     267

  Fragments arranged by size: 

                 792     714     645     581     454     453     435     383
                 267     207     206     175     171     145     132     102
                  42      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1012    1035    1687    5164    5931 
   Size:       1012      23     652    3477     767

  Fragments arranged by size: 

                3477    1012     767     652      23
 
TatI w'GTAC_w

Cuts at:      0    1049    1483    2907    2916    5931 
   Size:       1049     434    1424       9    3015

  Fragments arranged by size: 

                3015    1424    1049     434       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2183    2215    2782    2814    5931 
   Size:       2183      32     567      32    3117

  Fragments arranged by size: 

                3117    2183     567      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3211    5931 
   Size:       3211    2720
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     375    1851    2520    2628    2745    5826    5931 
   Size:        375    1476     669     108     117    3081     105

  Fragments arranged by size: 

                3081    1476     669     375     117     108     105
 
XhoI C'TCGA_G

Cuts at:      0     766    5735    5931 
   Size:        766    4969     196

  Fragments arranged by size: 

                4969     766     196
 
XmaI C'CCGG_G

Cuts at:      0      88    5931 
   Size:         88    5843
 
XmnI GAAnn'nnTTC

Cuts at:      0     277    3391    5931 
   Size:        277    3114    2540

  Fragments arranged by size: 

                3114    2540     277
 
ZraI GAC'GTC

Cuts at:      0    1348    5931 
   Size:       1348    4583
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI   AflIII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NdeI     NsiI     NspI     PasI    PflMI     PfoI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI
    PvuII     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SmaI     SmlI    SnaBI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI    AflII     AloI     AscI     AseI    AsiSI     BaeI
     BlpI     BmtI    BsiEI    BsiWI   BssHII    BstBI     DraI     DrdI
     EagI    EcoRV     FseI    FspAI     HpaI     MfeI     MluI     NaeI
   NgoMIV     NheI     NotI     NruI     PacI     PciI     PmeI     PsiI
     PvuI    RsrII     SalI     SgfI    SgrAI     SpeI     SrfI     SspI
     SwaI     XbaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com