Restriction map for pF1KB4546
##################################
# NO CUT ENZYMES (   45 enzymes) #
##################################

AatII       AccIII      AscI        AvrII       BbeI        
BlnI        BseAI       BsiWI       BsmBI       BspEI       
BsrBI       Bst1107I    BstZ17I     CpoI        DrdI        
EagI        EclXI       Eco52I      EcoRV       FseI        
HpaI        KasI        MluI        MroI        NarI        
NotI        NruI        PacI        PmeI        PvuI        
RsrII       SalI        SapI        SbfI        SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AclI         6076  AACGTT               AA^CGTT
AflII        8598  CTTAAG               C^TTAAG
AhdI         4648  GACTTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI         6549  ATTAAT               AT^TAAT
AsnI         6549  ATTAAT               AT^TAAT
AspEI        4648  GACTTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        6629  TGCGCA               TGC^GCA
BbvCI          60  GCTGAGG              CCTCAGC(-5/-2)
BfrI         8598  CTTAAG               C^TTAAG
BlpI         4371  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     4371  GCTCAGC              GC^T[ACGT]AGC
BsiEI        9725  CGACCG               CG[GA][CT]^CG
BstBI        6517  TTCGAA               TT^CGAA
CelII        4371  GCTCAGC              GC^T[ACGT]AGC
Eam1105I     4648  GACTTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI         6629  TGCGCA               TGC^GCA
KspI         6104  CCGCGG               CCGC^GG
NheI         3501  GCTAGC               G^CTAGC
NspV         6517  TTCGAA               TT^CGAA
PshAI       10355  GACGAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        6549  ATTAAT               AT^TAAT
Psp1406I     6076  AACGTT               AA^CGTT
SacII        6104  CCGCGG               CCGC^GG
SfuI         6517  TTCGAA               TT^CGAA
StuI          762  AGGCCT               AGG^CCT
XbaI         5559  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Acc65I       3158  GGTACC               G^GTACC
             6320  GGTACC              
AgeI         1405  ACCGGT               A^CCGGT
             9727  ACCGGT              
Aor51HI        39  AGCGCT               AGC^GCT
             8048  AGCGCT              
Asp718I      3158  GGTACC               G^GTACC
             6320  GGTACC              
BcgI         8766  CGAGGACACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4629  GCATTTGCTTCG        
BglI         1021  GCCTGCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4759  GCCCAGTGGGC         
BssHII         67  GCGCGC               G^CGCGC
               69  GCGCGC              
BstEII        361  GGTAACC              G^GT[ACGT]ACC
             9400  GGTGACC             
BstPI         361  GGTAACC              G^GT[ACGT]ACC
             9400  GGTGACC             
DraI         5521  TTTAAA               TTT^AAA
             9364  TTTAAA              
DraIII       8745  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
            10057  CACGCTGTG           
Eco47III       39  AGCGCT               AGC^GCT
             8048  AGCGCT              
EcoO65I       361  GGTAACC              G^GT[ACGT]ACC
             9400  GGTGACC             
EcoRI        2507  GAATTC               G^AATTC
             8756  GAATTC              
EcoT22I       703  ATGCAT               ATGCA^T
             7470  ATGCAT              
HindIII      2139  AAGCTT               A^AGCTT
             8475  AAGCTT              
KpnI         3158  GGTACC               GGTAC^C
             6320  GGTACC              
NaeI         6340  GCCGGC               GCC^GGC
             8065  GCCGGC              
NdeI          734  CATATG               CA^TATG
             3138  CATATG              
NgoMIV       6340  GCCGGC               G^CCGGC
             8065  GCCGGC              
NsiI          703  ATGCAT               ATGCA^T
             7470  ATGCAT              
PaeR7I       3869  CTCGAG               C^TCGAG
             9974  CTCGAG              
PinAI        1405  ACCGGT               A^CCGGT
             9727  ACCGGT              
SacI          542  GAGCTC               GAGCT^C
             7571  GAGCTC              
SspI         6946  AATATT               AAT^ATT
             7790  AATATT              
XhoI         3869  CTCGAG               C^TCGAG
             9974  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  161 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        Alw44I      AlwI        
AlwNI       AocI        ApaI        ApaLI       ApoI        
Asp700I     AspHI       AspI        AvaI        AvaII       
BalI        BamHI       BanI        BanII       BbrPI       
BbsI        BbvI        BciVI       BclI        BcnI        
BfaI        BglII       BmyI        BpmI        BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BsrI        
BssKI       BssSI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr10I      Cfr13I      ClaI        DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MfeI        
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MunI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PflMI       
PleI        PmaCI       PmlI        PpuMI       PstI        
PvuII       RcaI        RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SexAI       SfaNI       SfcI        
SmlI        SspBI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XcmI        XhoII       
XmnI        
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